SCAVENGING ACTIVITY OF DICLOFENAC. INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS

Rojo, C; Alvarez Figueroa MJ; Soto M.; Cañete Á.; Pessoa-Mahana D.; Lopez-Alarcon, C

Abstract

The scavenging activity of diclofenac was estimated by its capability to bleach ABTS radical cation, and ORAC (oxygen radical absorbance capacity) methodology. In addition, GC-MS technique was used to establish if the lactamic derivative of diclofenac was formed as final product in the interaction with peroxyl radicals. Diclofenac bleached ABTS radical cation in a concentration-dependent way. In ORAC assays, diclofenac inhibited the consumption of fluorescein and c-phycocyanin mediated by peroxyl radicals. An ORAC-fluorescein, and ORAC-c-phycocyanin value of 0.3, and 0.004 was estimated, respectively. From the downcurvature observed in Stern-Volmer-like plots, we postulated that the low protection of c-phycocyanin is related to secondary damage of c-phycocyanin by the secondary radical of diclofenac. By GC-MS studies only a 2,6-dichloroaniline derivative was detected as product afterthe reaction with peroxyl radicals. © 2009 Sociedad Chilena de Química.

Más información

Título según WOS: SCAVENGING ACTIVITY OF DICLOFENAC. INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
Título según SCOPUS: Scavenging activity of diclofenac. Interaction with ABTS radical cation and peroxyl radicals
Título según SCIELO: SCAVENGING ACTIVITY OF DICLOFENAC: INTERACTION WITH ABTS RADICAL CATION AND PEROXYL RADICALS
Título de la Revista: JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
Volumen: 54
Número: 1
Editorial: 2013
Fecha de publicación: 2009
Página de inicio: 58
Página final: 62
Idioma: English
DOI:

10.4067/S0717-97072009000100014

Notas: ISI, SCIELO, SCOPUS