Participation of Campylobacter jejuni flageller epitopes in cellular adhesion Participacion de epitopes flagelares de Campylobacter jejuni en la adhesion celular

Andrews E.; Fernandez, H; Folch H.; Eller, G; Pollette M.

Keywords: electron, isolation, adhesion, purification, infections, antibody, infection, cell, ultrastructure, microscopy, humans, human, male, campylobacter, pathogenicity, epitopes, infant, article, jejuni, immunology, microbiology, monoclonal, flagella, epitope, and, Antibodies,, Microscopy,, gram, negative, flagellum


Using a flagellated (052) and an aflagellated (T-1) strains we studied the participation of flagellar epitopes of C. jejuni in the adhesion to HEp-2 cells in vitro. Strain 052 was significantly more adherent than strain T-1. When adhesion assays were carried out with antiflagella monoclonal antibodies, strain 052 showed inhibition of their adhesive capacity that varied between 64.3 and 92.9%. With an ELISA test it was demonstrated that those monoclonal antibodies were specific and directed exclusively against flagellar epitopes of strain 052 being unreactive with strain T-1. Our results show that flagellar epitopes could participate in the adhesion process suggesting that flagella could be involved in the installation of the infectious process.

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Título de la Revista: MEDICINA-BUENOS AIRES
Volumen: 56
Número: 5 I
Fecha de publicación: 1996
Página de inicio: 487
Página final: 492