Human placental ecto-enzymes: Studies on theplasma membrane anchorage and effect of inhibitors of ATP-metabolizing enzymes
Keywords: inhibition, enzyme, membrane, placenta, degradation, binding, protein, cell, caffeine, alkaline, apyrase, humans, human, nucleotides, female, lidocaine, inhibitors, article, phosphatase, 5'-nucleotidase, analysis, c, phospholipase, microvilli, fluphenazine, priority, journal, D, phosphatidylinositol, Endopeptidases, amitriptyline, Glycosylphosphatidylinositols, Diacylglycerol-Lyase
The human placental microvillar membrane contains several ectoenzymes, including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydrolase (ATP-DPH), which might be involved in the extracellular metabolism of nucleotides. The type of anchorage to the plasma membrane of the two first enzymes has been shown to be via a glycosyl-phosphatidylinositol. In the present study, using an enzymatic approach, we show that the ATP-DPH should be attached to the plasma membrane through a different type of anchorage. We were also interested in the search of compounds which could interact differentially with this enzyme to be used as a tool for studying the other two hydrolytic enzymes in the presence of ATP-DPH. Here we report several inhibitors of ecto-ATPases which seem to be a useful tool for studying these three enzymes.
|Título de la Revista:||Research Communications in Molecular Pathology and Pharmacology|
|Editorial:||P J D PUBLICATIONS LTD|
|Fecha de publicación:||1997|
|Página de inicio:||14|