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Brian Alexander Effer Roldán

Postdoctorado

Universidad de La Frontera

Temuco, Chile

Líneas de Investigación


Producción de anticuerpos monoclonales de uso biotecnológico Bibliotecas génicas Fago display Ingeniería genética Bioquímica de proteínas Expresión y edición de proteínas recombinantes

Educación

  •  Biología Celular y Molecular Aplicada, UNIVERSIDAD DE LA FRONTERA. Chile, 2019
  •  Tecnología Bioquímica Farmacéutica, UNIVERSIDADE DE SAO PAULO. Brasil, 2019

Experiencia Académica

  •   Docente de Biotecnología Aplicada Part Time

    UNIVERSIDAD DE LA FRONTERA

    Facultad de Ingeniería y Ciencias

    Temuco, Chile

    2020 - At present

  •   Docente de Biomedicina y Diagnostico Molecular Part Time

    UNIVERSIDAD DE LA FRONTERA

    Facultad de Ciencias Agropecuarias y del Medio Ambiente

    Temuco, Chile

    2020 - At present

Experiencia Profesional

  •   Investigador Full Time

    Proyecto FONDEF D10I1064

    TEMUCO, Chile

    2012 - 2014

  •   Investigador Full Time

    Proyecto FONDECYT 1120705

    Temuco, Chile

    2012 - 2014

  •   Tesista Full Time

    Proyecto FAPESP 3/08617-7

    Sao Paulo, Brasil

    2015 - 2019

  •   Investigador Principal Full Time

    Proyecto FONDECYT de postdoctorado 3210142

    Temuco, Chile

    2021 - At present


 

Article (20)

Construction of a Human Immune Library from Gallbladder Cancer Patients for the Single-Chain Fragment Variable (<i>scFv</i>) Antibody Selection against Claudin 18.2 via Phage Display
Cathepsin B Is Not an Intrinsic Factor Related to Asparaginase Resistance of the Acute Lymphoblastic Leukemia REH Cell Line
Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems
Downstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic cancer cells and the bone marrow tumor microenvironment
Therapeutic Targets of Monoclonal Antibodies Used in the Treatment of Cancer: Current and Emerging
A low-cost system for the study of proteins used in salmonid diets, use of proteolysis to determine the quality
Increased glycosylated L-asparaginase production through selection of Pichia pastoris plataform and oxygen-methanol control in fed-batches
Increased glycosylated Lasparaginase production through selection of pichia pastoris plataform and oxygenmethanol control in fed-batches
Structure of the Mycobacterium tuberculosis cPknF and conformational changes induced in Forkhead-Associated regulatory domains
Glycosylation of Erwinase results in active protein less recognized by antibodies
Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis
A structural in silico analysis of the immunogenicity of L-asparaginase from Escherichia coli and Erwinia carotovora
L-Asparaginase from E. chrysanthemi expressed in glycoswitch: effect of His-Tag fusion on the extracellular expression
Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
Short-term cold storage of the semen of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) incorporating DMSO in the sperm diluent. Effects on motility and fertilizing capacity
Morphometric of blastomeres in Salmo salar
Gamete quality in fish: Evaluation parameters and determining factors
Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility
Study of the first blastomeres in Coho salmon (Oncorhynchus kisutch)
Morphometric characterization of the first blastomeres of rainbow trout (Oncorhynchus mykiss)

Proyecto (6)

A versatile platform to produce monoclonal antibodies (mAbs) against biomarkers overexpressed in the most lethal cancers: Towards the national sustainable production of proteins with biopharmaceutical and biotechnological purpose
Librerías génicas: Una plataforma versátil para obtener anticuerpos contra la tuberculosis en humanos.
Setting up de una plataforma de Phage – ribosome display para la obtención de anticuerpos monoclonales para cáncer de vesícula biliar: Nueva fuente de aplicación diagnóstica y de terapia
Produção de L-asparaginase extracelular=> da bioprospecção à engenharia de um biofármaco antileucêmico
Optimización del manejo artificial de la reproducción de salmonidos utilizando la criobiología como herramienta biotecnológica
Study of the physiological and genetic aspects related with gamete aging in salmonids.

Review (1)

From Synthesis to Characterization of Site-Selective PEGylated Proteins
24
Brian Effer

Postdoctorado

Centro de Excelencia en Medicina Translacional

Universidad de La Frontera

Temuco, Chile

6
Nemesio Valdebenito

PROFESOR TITULAR

Recursos Naturales

UNIVERSIDAD CATÓLICA DE TEMUCO

Temuco, Chile

4
Jorge Farias

Full Professor

Chemical Engineering

Universidad de La Frontera

Temuco, Chile

3
Elías Figueroa

Associate Professor.

Department of Biological and Chemical Sciences

Catholic University of Temuco

Temuco, Chile

2
Lisandra Herrera

Docente de planta

Universidad Santo Tomás

Temuco, Chile

1
Jennie Risopatrón

Académico y Director del CEBIOR

Ciencias Básicas

Universidad de La Frontera

Temuco, Chile

1
Osvaldo Merino

Investigador

Universidad de La Frontera

Temuco, Chile

1
Sindy Cabarca

Investigador Postdoctoral

Centro de Excelencia en Medicina Traslacional

Universidad de La Frontera

Temuco, Chile

1
Isabel Iturrieta

DOCENTE E INVESTIGADOR

CIENCIAS PRECLÍNICAS

Universidad de La Frontera

TEMUCO, Chile