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Eduardo Felipe Fuentes Lemus

Líneas de Investigación


Protein oxidation; Protein glycation; Protein cross-linking; Reactive oxygen species; LC-MS proteomics; Kinetics and mechanisms of oxidative damage to proteins in crowded environments

Educación

  •  Doctor en Química, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2018
  •  Químico Farmacéutico, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2014

Experiencia Académica

  •   Postdoc Full Time

    UNIVERSIDAD DE SANTIAGO DE CHILE

    Facultad de Química y Biología

    Chile

    2019 - 2020

  •   Postdoc Full Time

    University of Copenhagen

    Faculty of Health and Medical Sciences

    Copenhagen, Dinamarca

    2020 - At present

Premios y Distinciones

  •   PREMIO A LA EXCELENCIA EN TESIS DOCTORAL - VRI

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Chile, 2018

    Este premio tiene por objetivo reconocer y estimular la investigación desarrollada por alumnos/as de los distintos Programas de Doctorado de la Universidad, cuyas tesis doctorales representen un aporte a la proyección de las ciencias, humanidades, artes y tecnologías de nuestro país. Area Premio: Ciencias Químicas, Físicas y Matemáticas.

  •   SFRR-EUROPE CATHERINE PASQUIER MEMORIAL AWARD

    Society for Free Radical Research Europe

    Dinamarca, 2024

    The Catherine Pasquier Memorial Award Lecture is an annual award honouring the life of Catherine Pasquier of INSERM, Paris, and President of SFRR Europe from 2001-2002. The eligibility criteria have been selected to reflect what we know were Professor Catherine Pasquier’s priorities during her prolific career. The eligible person must be an Early Career Scientist: 1) Beyond formal training but not yet fully established. 2) From any country in Europe and an active member of SFRR Europe. 3) With career accomplishments and therefore also with future promise. 4) With achievements in the broad area of free radical research. This may include work at the basic, clinical or epidemiological level. This award is, thus, not intended for well-established investigators or as recognition for a single project, but for overall accomplishments and future promise. The required material are the candidate's full CV with an up-to-date publications list and a nominator's letter of recommendation. This letter is very important and should clearly lay out the importance of the candidate's accomplishments to date and be convincing in its prediction of future research promise. An SFRR Europe Catherine Pasquier Scientific Committee, comprised of the the SFRR Europe Executive and chaired by the President-Elect, selects the Award Lecture based on the received nominations with the awardee fulfilling the eligibility criteria, in particular their research excellence and promise and without regard to nationality.

  •   MSCA-IF-2019 - Individual Fellowships

    European Commission

    Chile, 2020

    EXCELLENT SCIENCE - Marie Sk?odowska-Curie Actions


 

Article (33)

Oxidation of the active site cysteine residue of glyceraldehyde-3-phosphate dehydrogenase to the hyper-oxidized sulfonic acid form is favored under crowded conditions
Complexation of AAPH (2,2'-azobis(2-methylpropionamidine) dihydrochloride) with cucurbit[7]uril enhances the yield of AAPH-derived radicals
Effect of crowding, compartmentalization and nanodomains on protein modification and redox signaling-current state and future challenges
From workout to molecular switches: How does skeletal muscle produce, sense, and transduce subcellular redox signals?
Oxidant-modified amylin fibrils and aggregates alter the inflammatory profile of multiple myeloid cell types, but are non-toxic to islet β cells
Oxygen Exposure and Tolerance Shapes the Cell Wall-Associated Lipids of the Skin Commensal Cutibacterium acnes
Peroxyl radicals modify 6-phosphogluconolactonase from Escherichia coli via oxidation of specific amino acids and aggregation which inhibits enzyme activity
The cysteine residue in beta-lactoglobulin reacts with oxidized tyrosine residues in beta-casein to give casein-lactoglobulin dimers
Crowding modulates the glycation of plasma proteins:<i> In</i><i> vitro</i> analysis of structural modifications to albumin and transferrin and identification of sites of modification
Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway
Oxidant-mediated modification and cross-linking of beta-2-microglobulin
Reaction of cysteine residues with oxidized tyrosine residues mediates cross-linking of photo-oxidized casein proteins
Role of amino acid oxidation and protein unfolding in peroxyl radical and peroxynitrite-induced inactivation of glucose-6-phosphate dehydrogenase from <i>Leuconostoc mesenteroides</i>
Effect of macromolecular crowding on protein oxidation: Consequences on the rate, extent and oxidation pathways
High concentrations of casein proteins exacerbate radical chain reactions and increase the extent of oxidative damage
M. jannaschii FtsZ, a key protein in bacterial cell division, is inactivated by peroxyl radical-mediated methionine oxidation
Oxidation of lysozyme induced by peroxyl radicals involves amino acid modifications, loss of activity, and formation of specific crosslinks
Azocompounds as generators of defined radical species: Contributions and challenges for free radical research
Formation and characterization of crosslinks, including Tyr-Trp species, on one electron oxidation of free Tyr and Trp residues by carbonate radical anion
Free radicals derived from ?-radiolysis of water and AAPH thermolysis mediate oxidative crosslinking of eGFP involving Tyr-Tyr and Tyr-Cys bonds: the fluorescence of the protein is conserved only towards peroxyl radicals
Photo-induced protein oxidation: mechanisms, consequences and medical applications
Photo-oxidation of lysozyme triggered by riboflavin is O2-dependent, occurs via mixed type 1 and type 2 pathways, and results in inactivation, site-specific damage and intra- and inter-molecular crosslinks
Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times
Binding of rose bengal to lysozyme modulates photooxidation and cross-linking reactions involving tyrosine and tryptophan
Oxidation of myofibrillar proteins induced by peroxyl radicals: Role of oxidizable amino acids
Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching
Riboflavin-induced Type 1 photo-oxidation of tryptophan using a high intensity 365 nm light emitting diode
Aggregation of alpha- and beta- caseins induced by peroxyl radicals involves secondary reactions of carbonyl compounds as well as di-tyrosine and di-tryptophan formation
alpha- and beta-casein aggregation induced by riboflavin-sensitized photo-oxidation occurs via di-tyrosine cross-links and is oxygen concentration dependent
Use of the Oxygen Radical Absorbance Capacity (ORAC) Assay to Predict the Capacity of Mango (Mangifera indica L.) By-Products to Inhibit Meat Protein Oxidation
Oxidation of free, peptide and protein tryptophan residues mediated by AAPH-derived free radicals: Role of alkoxyl and peroxyl radicals
Use of the Oxygen Radical Absorbance Capacity (ORAC) Assay to Predict the Capacity of Mango (Mangifera indica L.) By-Products to Inhibit Meat Protein Oxidation
The ORAC (oxygen radical absorbance capacity) index does not reflect the capacity of antioxidants to trap peroxyl radicals

Proyecto (4)

Estudio del rol de las mezclas de caseínas sobre el grado y mecanismo de oxidación de estas proteínas mediado por los radicales peroxilo
Oxidative modifications induced by carbonate radical, nitrogen dioxide, and peroxyl radicals on two enzymes of the pentose phosphate pathway=> consequences on NADPH release and reduced glutathione recycling
Role of Tryptophan residues in the aggregation and fragmentation of peptides and proteins induced by peroxyl and carbon-centered radicals.
Extending the use of Pyrogallol Red as probe in methodologies aimed to estimate the scavenging activity of beverages and foods towards Nitrite, Hypochlorous acid, Chloramines and Peroxyl radicals.

Review (2)

The enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species consequences for NADPH production
Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
12
Camilo López

prof. Asociado

Química Física

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

4
ALEXIS ASPEE

Profesor Titular

Ciencias del Ambiente

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

3
Pablo Barrías

Profesor por hora

Ciencias del Ambiente

Universidad de Santiago de Chile

Santiago, Chile

3
HERNAN SPEISKY

PROFESOR TITULAR

UNIVERSITY OF CHILE

SANTIAGO, Chile

3
EVA DORTA

Investigador Asociado

Química-Física

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Javiera Cortés

Estudiante de Doctorado

Instituto de Ingeniería Biológica y Médica

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Francisco Mura

Investigador postdoctoral

Química y Biología

Universidad de Santiago de Chile

Santiago, Chile

1
Alejandra Herrera

PROFESORA ASISTENTE

QUÍMICA Y FISICA

Universidad Autónoma de Chile

TALCA, Chile

1
Angelica Fierro

Associate

Chemistry

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Víctor Castro

Profesor Asistente

Departamento de Biología

UNIVERSIDAD DE CHILE

Ñuñoa, Chile

1
ANA ZÁRATE

investigadora

Químca-Física

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Denis Fuentealba

PROFESOR ASOCIADO

QUIMICA FISICA

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Felipe Avila

Profesor Asociado

UNIVERSIDAD DE TALCA

Talca, Chile

1
David Contreras

Full professor

Analitycal and Inorganic Chemistry

UNIVERSIDAD DE CONCEPCION

Concepción, Chile

1
Mario Faúndez

Profesor Asistente

Farmacia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Ricardo Zamora

Researcher Assistant

Universidad de Talca

Talca, Chile

1
Margarita Aliaga

Associate Professor

Química Física

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Eduardo Pino

Acádemico

Ciencias del Ambiente

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

1
María Rodríguez

Profesora Asociada

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Raquel Bridi

Profesor Asociado

Departamento de Química Farmacológica y Toxicológica

Universidad de Chile

Santiago, Chile

1
Andrea Rivas

Investigadora

Universidad San Sebastián

Santiago, Chile

1
Elias Atala

Head of Science & Technology

R & D

Q-VANT Bioscience

Santiago, Chile

1
Carlos Henríquez

Professor Asistente

Kinesiologia

Universidad Finis Terrae

Santiago, Chile