Man

Susana SAEZ RIVERA

Assistant Profesor

Universidad Andres Bello

Santiago, Chile

Líneas de Investigación


Characterization of proteins acting on Arabidopsis seed coat mucilage biosynthesis and modification; Biochemical analysis of plant cell wall.

Educación

  •  Biology, UNIVERSITÉ PIERRE-ET-MARIE-CURIE, PARIS VI. Francia, 2007
  •  Integrative and physiology biology, UNIVERSITÉ PIERRE-ET-MARIE-CURIE, PARIS VI. Francia, 2009
  •  Biología Vegetal, Institut Jean Pierre Bourgin, INRA (Versailles). Francia, 2012

Experiencia Profesional

  •   PhD- Characterization of an Arabidopsis accession affected in mucilage release Full Time

    INRA (Institut National de la Recherche Agronomique)

    Francia

    2009 - 2012

  •   Postdoctoral Post- Principal Investigator- Fondecyt Postdoc 3140415 Full Time

    Universidad Andres Bello

    Santiago, Chile

    2013 - 2016

  •   Principal Investigator- Fondecyt "Iniciación en Investigacion" Nº 11160787 Part Time

    Universidad Andres Bello

    Santiago, Chile

    2016 - A la fecha

  •   Principal Investigator- Fondecyt PAI 79170136 Part Time

    Universidad Andres Bello

    Santiago, Chile

    2017 - A la fecha

Formación de Capital Humano


Human capital training (last five years, 2014-2019)

Undergraduate thesis (Tutor): 1

- Ignacio Rios Muñoz (2019). Identificación de un probable inhibidor de pectin metilesterasa (PMEI3) implicado en el control de la metilesterificacion del homogalacturonano en el mucílago de semillas de Arabidopsis. Ingeniería en Biotecnología y Ciencias de la Vida, Universidad Andrés Bello.

Undergraduate thesis (scientific comittee): 2

- Sofia Muñoz (2018). Estudio del rol de los segundos mensajeros (Calcio y ROS) en la translocación al núcleo de la proteína AtbZIP60U de Arabidopsis thaliana, en condiciones de estrés osmótico. Ingeniería en Biotecnología y Ciencias de la Vida, Universidad Andrés Bello.
- Paula Sandoval (2018). Análisis de regiones diferencialmente metiladas asociadas a la acumulación de frío durante la dormancia en yemas florales de cerezo ( Prunus avium L.). Ingeniería en Biotecnología y Ciencias de la Vida, Universidad Andrés Bello.


Difusión y Transferencia


Conference presentations (2014-2019)

Celiz J, Rios I, Parra-Rojas J, Largo-Gosens A, Orellana A, Saez-Aguayo S. Identification of a new Pectin Methylesterase Inhibitor using the Arabidopsis Seed Coat Mucilage. Cell Wall Conference, Asilomar , California, USA, 18 to 21 june 2018. (Oral presentation).

Saez-Aguayo S; Sanhueza D; Ejsmentewicz T, Temple H, Reyes F, and Orellana A. Functiona characterization of Nucleotide Sugar Transporters (NSTs) by exploiting the Arabidopsis seed mucilage. Centro de Biotecnología Vegetal, UNAB. The 5th PanAmerican Plant Membrane Biology Workshop, 1st to 5th june 2015. (Oral presentation).

Saez-Aguayo S; Rautengarten C, Sanhueza D, Ejsmentewicz T, Omar Sandoval O, Mollet JC, Heazlewood J, Scheller H, Reyes F and Orellana A. UUAT1: an UDP-uronic acid transporter involved in cell wall composition.. The 26th International Conference on Arabidopsis Research (ICAR, Paris, France, 2015 (Oral presentation).

Poster presentations (2014-2019)

Rios-Muñoz I, Celiz J, Parra-Rojas JP, Sepulveda P, Orellana A, Saez-Aguayo S. Characterization of a putative pectin methylesterase PME51 using the Arabidopsis seed coat mucilage. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Parra-Rojas JP, Largo-Gosens A, Carrasco T, Celiz J, Sepúlveda P, Temple H, Sanhueza D, Reyes Francisca C, Meneses C, Saez-Aguayo S, Orellana A. Exploiting developing seeds RNA- seq to understand mucilage formation. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Celiz-Balboa J, Saez-Aguayo S, Parra-Rojas JP, Temple H, Arenas-Morales V, Ríos-Muñoz I, Largo-Gosens A, Sepúlveda P, Orellana A. Specificity and similarity of functions of UDP- Rhamnose/UDP- Galactose transporters, URGT1 and URGT2, in cell wall pectins biosynthesis by using a promotor swap approach. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Saez-Aguayo S, Parra-Rojas JP, Celiz-Balboa J, Sepúlveda P, Crépeau M-J, Largo-Gosens A, North HM, Ralet M-C, Orellana A. URGT2, URGT4 and URGT6 modulate Rhamnogalacturonan-1 length of seed coat Arabidopsis mucilage. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Celiz-Balboa J, Rios-Muñoz I, Parra-Rojas JP, Arenas- Morales V, Largo-Gosens A, Orellana A, Saez-Aguayo S. Identification of two pectin methylesterase inhibitors (PMEI) using the Arabidopsis seed coat mucilage. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Largo-Gosens A, Parra-Rojas JP, Celiz-Balboa J, Arenas-Morales V, Sepúlveda P, Rios- Muñoz I, Saez-Aguayo S, Orellana A. First steps to understand the topology of the synthesis of UDP- Arabinofuranose in plants. XIII Reunión de Biología Vegetal. Puerto Varas, Chile. December 3th- 6th, 2018.

Celiz J, Rios I, Parra-Rojas J, Largo-Gosens A, Orellana A, Saez-Aguayo S. Identification of pectin methylesterases (PME) and pectin methylesterases inhibitors (PMEI) using Arabidopsis seed coat mucilage.Centro de Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Largo-Gosens A, Saez-Aguayo S, Celiz J, Parra-Rojas J, Rios I, Orellana A. Understanding the topology of the synthesis of UDP-Arabinofuranose and its impact on the Arabidopsis seed coat mucilage biosynthesis. Centro de Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Celiz-Balboa J, Parra-Rojas J P, Orellana A, Saez-Aguayo S. PMEI13 as a new pectin methylesterase inhibitor implicated in mucilage homogalacturonan methylesterification.? Rios-Muñoz I, Centro de Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Nuñez P, Silva-Sanzana C, Saez-Aguayo S, Blanco-Herrera F. Dynamics of Homogalacturonans in aphid-plant interaction Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Celiz-Balboa J, Saez-Aguayo S, Parra-Rojas J, Temple H, Ríos-Muñoz I, Largo-Gosens A, Orellana A. Assesing UDP-Rhamnose/UDP-Galatose Transporters specific roles in Arabidopsis thaliana cell wall pectins biosynthesis by using a promotor swap approach. Centro de Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Parra-Rojas J, Saez-Aguayo S, Celiz-Balboa J, Largo-Gosens A, Rios-Muñoz I, Ralet M, North H, Orellana A. The key role from UDP-Rhamnose/Galactose Transporters in the biosynthesis of seed coat mucilage. Centro de Biotecnología Vegetal, UNAB. XII Reunión de Biología Vegetal. Villarica, Chile. December 4th- 7th, 2017.

Temple H, Celiz-Balboa J, Saez-Aguayo S, Parra J, Dupree P and Orellana A. Role of UDP- Uronic Acids Transporters in The Biosynthesis of non.-cellulosic polysaccharides in Arabidopsis thaliana. Centro de Biotecnología Vegetal, UNAB. XI Reunión de Biología Vegetal. Chillán, Chile. November 28th- December 1th, 2016.

Saez-Aguayo S; Parra JP; Sanhueza D; Ejsmentewicz T ; Temple H ; and Orellana A. Phenotipic and biochemical characterization of mutants affected in the UDP-Rhamnose/ Galactose Transporters. Centro de Biotecnología Vegetal, UNAB. X Reunión de Biología Vegetal. Valdivia, Chile. December 1st- 5th, 2015.

Silva-Sanzana C, Saez-Aguayo S, Urra C, Arraño P, Andrade D, Ahumada I, Chorbadjian R, Blanco-Herrera F. Centro de Biotecnología Vegetal, UNAB. Role of cell Wall and intercelular polymers during aphid-plant interaction. X Reunión de Biología Vegetal. Valdivia, Chile. December 1st- 5th, 2015.

Saez-Aguayo S; Sanhueza D; Ejsmentewicz T; Doñas D; Reyes F-C and Orellana A. UAAT1: A nucleotide sugar transporter (NSTs) involved in seed coat , trichome and root cell Wall composition. Centro de Biotecnología Vegetal, UNAB. IX Reunión de Biología Vegetal. La Serena, Chile. December 1st- 4th, 2014.

Outreach activities (2014-2019)

2018 – Coordinator of the first French-Chilean cell wall workshop, with the participation Marie-Christine Ralet, INRA de Nantes and Jérôme Pelloux, Universtité de Picardie, Universidad Andrés Bello, Santiago (3th -4th October 2018).

2017 – Talk “Elucidation of Pectin Biosynthesis Using the Arabidopsis Seed Coat Mucilage”, Plant cell wall and parietal polysaccharides research group (PVPP), BIA, INRA, Nantes , France (10th November 2017).



 

Article (8)

The elaborate route for UDP-arabinose delivery into the Golgi of plants.
UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage.
The inside and outside: topological issues in plant cell wall biosynthesis and the roles of nucleotide sugar transporters.
Local evolution of seed flotation in Arabidopsis.
The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter family of Arabidopsis.
Understanding polysaccharide production and properties using seed coat mutants: future perspectives for the exploitation of natural variants.
PECTIN METHYLESTERASE INHIBITOR6 promotes Arabidopsis mucilage release by limiting methylesterification of homogalacturonan in seed coat epidermal cells.
Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves.

Proyecto (3)

El mucilago de Arabidopsis, excelente modelo para el estudio de la biosíntesis y modificación de la pared cellular
Characterization of pectin methylesterases (PMEs) and pectin methylesterases inhibitors (PMEIs) acting on homogalacturonan methylesterification in Arabidopsis seed coat mucilage
Functional characterization of Nucleotide Sugar Transporters (NSTs) by exploiting the Arabidopsis seed mucilage

Article (1)

Homogalacturonan status modulation through PME/PMEI13 is a key component of Arabidopsis defense during the early stage of infestation by Myzus persicae
4
Susana SAEZ

Assistant Profesor

Centro de Biotecnología Vegetal

Universidad Andres Bello

Santiago, Chile