Foto_raul_sanchez.png_thumb90

Raul Sanchez Gutierrez

DIRECTOR DE CENTRO DE EXCELENCIA

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

Líneas de Investigación


Fisiopatologia de los procesos inflamatorios que afectan el aparato reproductor y su efecto sobre la funcion espermatica Criopreservacion de espermatozoides y efecto sobre parametros reproductivos

Educación

  •  Medico cirujano, UNIVERSIDAD DE CHILE. Chile, 1981
  •  Ginecologia y Obstetricia, UNIVERSIDAD DE LA FRONTERA. Chile, 1984
  •  Andrologia, JUSTUS-LIEBIG UNIVERSITAT GIESSEN. Alemania, 1993
  •  Medicina Reproductiva, Conacem. Chile, 2017

Experiencia Académica

  •   PROFESOR TITULAR Full Time

    UNIVERSIDAD DE LA FRONTERA

    MEDICINA

    TEMUCO, Chile

    1981 - A la fecha

Experiencia Profesional

  •   VICERRECTOR DE INVESTIGACION Y POSTGRADO Full Time

    UNIVERSIDAD DE LA FRONTERA

    TEMUCO, Chile

    2006 - 2014

  •   Director de Centro de Excelencia Part Time

    UNIVERSIDAD DE LA FRONTERA

    Chile

    2016 - A la fecha

  •   Becario/Investigador Full Time

    Alexander Von Humboldt

    Munich, Alemania

    1987 - 1989

Formación de Capital Humano


1. Patricia Navarrete Gómez, Tesis: “Acción moduladora del oviducto y sus secreciones sobre el efecto de la aracnotoxina de latrodectus mactans en la función espermática”. Doctorado en Ciencias, mención Biología celular y molecular aplicada, Universidad de la Frontera, 2011.
2. Washington Efraín Aguagüiña González, Tesis: “Efecto de antioxidantes sobre la producción de ero y función espermática en la vitrificación aséptica en humano”, Magíster en Ciencias, mención Biología de la reproducción, Universidad de la Frontera, 2010.
3. Olga Teresa Berríos Calvo, Tesis; “Efectos de la vitrificación sobre la función espermática en donantes normozoospérmicos”. Magíster en ciencias mención Biología de la reproducción, Universidad de la Frontera, 2008.
4. Maria Elena Arias Cea, tesis: “Optimización de la técnica de inyección intracitoplasmatica de espermatozoides (icsi) en bovinos y evaluación de la calidad de los embriones generados”. Doctorado en Ciencias, mención Biología celular y molecular aplicada, Universidad de la Frontera, 2013.
5. Carolina Cheuqueman Arevalo, Tesis: “Modulación del estrés oxidativo sobre la producción de embriones in vitro: efecto de melatonina e inducción de tolerancia a estrés oxidativo en ovocitos de bovino”. Doctorado en Ciencias, mención Biología celular y molecular aplicada, Universidad de la Frontera, 2014.
6. Pia Loren Reyes. Tesis: “Exposición de complejos cúmulo–ovocitos bovinos a inductores de estrés oxidativo y nitrosativo: inducción de tolerancia y su efecto sobre la sobrevivencia de embriones producidos in vitro post–criopreservación”. Doctorado en Ciencias, mención Biología celular y molecular aplicada, Universidad de la Frontera, 2020.
7. Mabel Schulz Rubilar. Tesis: “Efecto del daño espermático inducido por criopresevación y bacterias urogenitales sobre la producción de trampas extracelulares de leucocitos (ETs) durante su interacción con espermatozoides humanos”. Doctorado en Ciencias Morfológicas, Universidad de la Frontera, 2020.
8. Carla Rubilar Fincheira. Tesis: "Efecto de la suplementación con Calafate (Berberis microphylla) sobre marcadores metabólicos y alimentarios en ratas con obesidad inducida por la dieta". Doctorado de Ciencias Medicas, Universidad de La Frontera, 2020.


Premios y Distinciones

  •   MIEMBRO CORRESPONDIENTE DE LA ACADEMIA DE MEDICINA DE CHILE

    ACADEMIA CHILENA DE MEDICINA

    Chile, 2015

    Miembro Correspondiente Nacional

  •   MIEMBRO CORRESPONDIENTE DE LA ACADEMIA NACIONAL DE MEDICINA DE BRASIL

    ACADEMIA NACIONAL DE MEDICINA DE BRASIL

    Brasil, 2016

    Miembro Correspondiente Internacional


 

Article (153)

Antioxidant effects of penicillamine against in vitro-induced oxidative stress in human spermatozoa
Human sperm vitrification: A scientific report
Increase of leukocyte-derived extracellular traps (ETs) in semen samples from acute epididymitis patients– a pilot study
Induction of oxidative stress does not increase the cryotolerance of vitrified embryos
Multiparameter flow cytometry assay for analysis of nitrosative stress status in human spermatozoa
Protective effect of the superoxide dismutase mimetic MnTBAP during sperm vitrification process
Rest days and storage of boar semen at 17 degrees C: Effect on motility and sperm concentration
Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo) in infertile patients-A pilot study
Effect of high-fat and vitamin D deficient diet on rat sperm quality and fertility
LED-based red light photostimulation improves short-term response of cooled boar semen exposed to thermal stress at 37 degrees C
Monocyte-derived extracellular trap (MET) formation induces aggregation and affects motility of human spermatozoa in vitro
Positive effect of butylated hydroxytoluene (BHT) on the quality of cryopreserved cat spermatozoa
Changes in sperm function and structure after freezing in domestic cat spermatozoa
Concentrations of non-permeable cryoprotectants and equilibration temperatures are key factors for stallion sperm vitrification success
Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis
Chapter 6 Technology of Aseptic Cryoprotectant-Free Vitrification of Human ICSI Spermatozoa.
Decrease of bovine in vitro embryo production (IVP) efficiency during winter season in a warm-summer Mediterranean climate.
Effect of incubation temperature after devitrification on quality parameters in human sperm cells
Effect of sperm pretreatment with glutathione and membrane destabilizing agents Lysolecithin and Triton X-100, on the efficiency of bovine intracytoplasmic sperm injection (ICSI).
Effect of the age of broodstock males on sperm function during cold storage in the trout ( Oncorhynchus mykiss )
Melatonin Scavenger Properties against Oxidative and Nitrosative Stress=> Impact on Gamete Handling and In Vitro Embryo Production in Humans and Other Mammals
Nitrosative stress by peroxynitrite impairs ATP production in human spermatozoa
Technologies of cryoprotectant-free vitrification of human spermatozoa=> asepticity as criterion of effectiveness
Trehalose sustains a higher post-thaw sperm motility than sucrose in vitrified human sperm.
Determination of fatty acid profile in ram spermatozoa and seminal plasma.
Short-term storage of salmonids semen in a sodium alginate-based extender.
Effect of anisomycin, a protein synthesis inhibitor, on the in vitro developmental potential, ploidy and embryo quality of bovine ICSI embryos.
Effect of short-term exposure of cumulus-oocyte complex to 3-morpholinosydnonimine on in vitro embryo development and gene expression in cattle.
Improved preimplantation development of bovine ICSI embryos generated with spermatozoa pretreated with membrane-destabilizing agents lysolecithin and Triton X-100.
Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility.
Modulación del Estado de Óxido-Reducción por Peróxido de Hidrógeno en la Etapa de Maduración Ovocitaria=> Efecto sobre el Desarrollo Embrionario en Bovinos
Short-term storage of salmonids semen in a sodium alginate-based extender
Thiol oxidation by nitrosative stress: Cellular localization in human spermatozoa
Use of the fluorescent dye TMRM for mitochondrial membrane potential assessment in human spermatozoa
Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants.
Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification.
Effects of short-term exposure of mature oocytes to sodium nitroprusside on in vitro embryo production and gene expression in bovine.
High temperature is essential for preserved human sperm function during the devitrification process.
Infecciones de transmisión sexual silentes: la muestra de orina permite una adecuada detección.
Intracytoplasmic sperm injection affects embryo developmental potential and gene expression in cattle
N-acetyl-L-cysteine pre-treatment protects cryopreserved bovine spermatozoa from reactive oxygen species without compromising the in vitro developmental potential of intracytoplasmic sperm injection embryos
Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa.
Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique.
Addition of superoxide dismutase mimics during cooling process prevents oxidative stress and improves semen quality parameters in frozen/thawed ram spermatozoa.
Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry.
Effect of sperm pretreatment with NaOH and DTT on the efficiency of bovine intracytoplasmic sperm injection.
Human Papilloma Virus Frequency in non-Gynecological Tumors in the Araucania Region, Chile
Identification of fatty acids in canine seminal plasma.
Quality of human spermatozoa: relationship between high-magnification sperm morphology and DNA integrity.
Sperm Selection in Frozen/Thawed Semen of Equine: Evaluation of Plasma, Acrosome Membranes and Mitochondrial Membrane Potential
Supplementation of IVF medium with melatonin: effect on sperm functionality and in vitro produced bovine embryos.
Validation of SYBR-14 and 6-CFDA to Evaluate the Viability and Plasma Membrane Integrity in Sperm of Chihuahua Canine Breed
Stability of reference genes for normalization of reverse transcription quantitative real-time PCR (RT-qPCR) data in bovine blastocysts produced by IVF, ICSI and SCNT
[Detection of human papilloma virus infection in men].
[Frequency of Chlamydia trachomatis infection in a group of women from Region of Araucanía, Chile].
Assessment of sperm function parameters and DNA fragmentation in ejaculated alpaca sperm (Lama pacos) by flow cytometry.
Criopreservación de Espermatozoides Caninos a - 80C
Diagnóstico de la infección por virus papiloma humano en el hombre
Effect of the addition of two superide dismutase anologues (Tempo and Tempol) to alpaca semen extender for cryopreservation.
Frecuencia de la infección por Chlamydia trachomatis en un grupo de mujeres de la Región de la Araucanía, Chile
Infecciones de Transmisión Sexual en Semen. El Hombre como Vector de Transmisión
Infección por el virus del papiloma humano en el hombre y su relación con el cáncer: estado actual y prospectivas
Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Stability of reference genes for normalization of reverse transcription quantitative real-time PCR (RT-qPCR) data in bovine blastocysts produced by IVF, ICSI and SCNT.
Vitrification sperm: An alternative to intracytoplasmic sperm injection in oligoasthenozoospermic patient
Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa=> first report.
Criopreservación de semen canino con diferentes fracciones proteicas del fluido seminal
Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report.
Effect of aracnotoxin from Latrodectus mactans on bovine sperm function: modulatory action of bovine oviduct cells and their secretions.
Effect of tubal explants and their secretions on bovine spermatozoa: modulation of ROS production and DNA damage
Effects of seminal fluid fraction on plasma and acrosome membrane integrity and mitochondrial membrane potential determined by flow cytometry in chilled canine spermatozoa.
Evaluación de la integridad de ADN mediante citometría de flujo en espermatozoides de alpaca criopreservados con análosos de superóxido dismutasa
Evaluation of different culture systems with low oxygen tension on the development, quality and oxidative stress-related genes of bovine embryos produced in vitro.
Human spermatozoa vitrified in the absence of permeable cryoprotectants: birth of two healthy babies.
Live Birth After Intrauterine Insemination With Spermatozoa From an Oligoasthenozoospermic Patient Vitrified Without Permeable Cryoprotectants
Neural differentiation of rat aorta pericyte cells.
Sperm membrane functionality in the dog assessed by flow cytometry.
Vitrification of Human ICSI/IVF Spermatozoa Without Cryoprotectants: New Capillary Technology
Vitrified sperm banks: the new aseptic technique for human spermatozoa allows cryopreservation at -86 °C.
Evaluation of different culture systems with low oxygen tension on the development, quality and oxidative stress-related genes of bovine embryos produced in vitro.
Canine sperm vitrification with sucrose: effect on sperm function.
Congelación Ultra Rápida de Espermatozoides Humanos: Efecto sobre la Función Espermática y Producción de Especies Reactiva de Oxígeno
Cryoprotectant-Free Vitrification of Human Spermatozoa in Large (to 0.5 mL) Volume=> Novel Technology.
Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: stability of mitochondrion as criterion of effectiveness.
Los Pericitos: Nuevos Enfoques en la Terapia Regenerativa, Patología Cerebrovascular y Cáncer
Molecular characterization, electrophysiological and contraceptive effect of chilean latrodectus venom
Participation of the sperm proteasome during in vitro fertilisation and the acrosome reaction in cattle.
Proteasome activity and proteasome subunit transcripts in human spermatozoa separated by a discontinuous Percoll gradient.
Effect of E. coli or of its soluble factors on mitochondrial membrane potential, phosphatidylserine translocation, viability and motility in human spermatozoa.
Human sperm chemotaxis depends on critical levels of reactive oxygen species
Progesterone sperm chemoattraction may be modulated by its corticosteroid-binding globulin carrier protein.
Tetraethylammonium-sensitive K(+) current in the bovine spermatozoa and its blocking by the venom of the Chilean Latrodectus mactans.
Trypsin and chymotrypsin are involved in the progesterone-induced acrosome reaction in canine spermatozoa.
Venom of the Chilean Latrodectus mactans alters bovine spermatozoa calcium and function by blocking the TEA-sensitive K(+) current.
Epiblast Embryo Stem Cells Give Origin to Adult Pluripotent Cell Populations: Primordial Germ Cell, Pericytic and Haematopoyetic Stem Cells. A Review
Integrity of mitochondrial membrane potential reflects human sperm quality
Molecular mechanism for human sperm chemotaxis mediated by progesterone
Protective effect of Fallopian tubal fluid against activated leukocyte-induced sperm DNA fragmentation: preliminary results
Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose.
Chemotactic response of frozen-thawed bovine spermatozoa towards follicular fluid.
Effect of Protease Inhibitors on the Acrosome Reaction and Sperm-Zona Pellucida Binding in Bovine Sperm
Potassium Current in Mature Bovine Spermatozoa
Effects of arachnotoxin on intracellular pH and calcium in human spermatozoa
A new technique to evaluate the ability of cryoprotectors to prevent premature acrosome reaction in human spermatozoa
Progesterone at the picomolar range is a chemoattractant for mammalian spermatozoa.
Bacteria induce expression of apoptosis in human spermatozoa
Effect of different concentrations of heparin on canine sperm capacitation in vitro
Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders
Influence of reactive oxygen species produced by activated leukocytes at the level of apoptosis in mature human spermatozoa
Alpha-glucosidase in the human epididymis: topographic distribution and clinical application.
Detection of a CBG-like protein in human Fallopian tube tissue
DISPOSITIVOS INTRAUTERINOS CON LEVONORGESTREL: UNA NUEVA ALTERNATIVA TERAPÉUTICA EN EL SANGRADO UTERINO ANORMAL DE ORIGEN ORGÁNICO
Effect of lysophosphatidyicholine on acrosome reaction in canine spermatozoa
Effect of temperature incubation and glycerol addition on the acrosome preservation in human spermatozoa
Update on the impact of Chlamydia trachomatis infection on male fertility
Determinación de células peroxidasa positivas en líquido seminal: ¿es un parámetro confiable para el diagnóstico de infección genital asintomática?
Lack of species-specificity in mammalian sperm chemotaxis
Reactive oxygen species induce reversible capacitation in human spermatozoa
Urogenital inflammation: changes of leucocytes and ROS
Effect of albumin and polyvinyl alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa incubated in vitro.
Endometrio con reacción decidual idiopática en una mujer postmenopáusica: evaluación inmunocitoquímica de receptores de estrógeno, de progesterona y de su proteína transportadora (CBG)
Indirect immunofluorescence using monoclonal antibodies for the detection of leukocytospermia: comparison with peroxidase staining
Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
Evaluation of the acrosome reaction in human spermatozoa: comparison of cytochemical and fluorescence techniques.
Chromatin condensation and seminal alpha glucosidase variability and their relationship to spermiogram.
Localización inmunocitoquímica de la serpina CBG-símil en el sistema reproductor de mamíferos hembra
Use of Midodrine and frozen/thawed semen to treat semen transport disturbances. Report of two cases
Evidence fot the synthesis and secretion of a CBG-like serpin by human cumulus oophorus and fallopian tubes.
Production and characterization of monoclonal antibodies to the major protein of boar outer dense fibers
Differentiation of ejaculates showing reactive oxygen species production by spermatozoa o leukocytes.
Glass wool filtration reduces reactive oxygen species in oligozoospermic patients with leukocytospermia.
Migration /sedimentation sperm selection method used in bovine IVF: comparison with washing/ centrifugation.
Sperm selection methods for intracytoplasmic sperm injection (ICSI) in andrological patients.
Comparison of two methods to obtain hemizona pellucida for sperm function tests
Evaluation of the acrosome in oligozoospermic patients by simplified triple stain technique
Can anesthesia influence sperm motility in gamete intrafallopian transfer (GIFT)?
Evaluation of the acrosomal membrane in bovine spermatozoa: effects of proteinase inhibitors.
Pregnancy and birth after intracytoplasmatic sperm injection with spermatozoa from a patient with tail stump syndrome.
Evaluation of nuclear maturity in human spermatozoa obtained by sperm preparation method.
Selección de espermatozoides de bovino desde semen congelado-descongelado: comparación de dos métodos
Demonstration of acrosomal membranes using the hypoosmotic swelling test.
Acrosome reaction induced by low temperature increases fusion rate in zona-free hamster ova.
Influence of incubation time/temperature on acrosome reaction/sperm penetration assay.
Examen de fusión gamética y su aplicación en la clínica andrológica
Induction of the acrosome reaction in sperm by exposure to low temperature increases their rate of fusion with zona-free hamster oocytes.
The in vitro interactions of eutherian ova and spermatozoa and their significance for orientation and binding of the gametes.
A new method for evaluation of the acrosome reaction in viable human spermatozoa.
Ultrastructural analysis of the attachment sites of Escherichia coli to the human spermatozoon after in vitro migration through estrogenic cervical mucus.
Inhibition of stress-induced hyperglycemia by tail pinching or intraventricular enkephalin administration in the rat.
[Mollicutes in male infertility: is antibiotic therapy indicated?].

BookSection (6)

Sperm Chemotaxis in Mammals
Infección e Infertilidad, un enfoque fisiopatológico
Vitrification Technique – New Possibilities for Male Gamete Low-Temperature Storage
Vitrificacion de Espermatozoides
Cryppreservation of spermatozoa: Old routine and new perspectives
Cryprotectant-free vitriification of spermatozoa

Patent (3)

Method for vitrification human semen and portable kit for aplication of said method
Method for cryopreservation of human spermatozoa free from seminal plasma using a fast and simple aseptic vitrification- devitrification process: portable kit for carrying out the method; and use of the same for treatment of disorders related to reproductive failures
Péptidos derivados del veneno de la araña latrodectus mirabilis, secuencias nucleotídicas para transformar un microorganismo capaz de producir los péptidos; métodos para obtener los péptidos; composiciones farmacéuticas que los contienen y usos de los mismos

Proyecto (11)

Development of a boar semen cryopreservation medium by modulating oxidative/nitrosative stress and neutrophil extracellular traps (NETs)=> Impact on sperm function andreproductive outcome
Evaluation of the antioxidant FeTPPS on human spermatozoa undergoing nitrosative stress in vitro
Capacitation of stallion sperm=> Effects on the in vitro production of embryos generated by conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI)
Efecto de las alteraciones metabólicas en la reproducción masculina=> Estudio Clínico y Experimental
Detection Kit silent sexually transmitted infections in urine samples. A noninvasive diagnostic tool and inexpensive.
INDUCTION AND MODULATION OF OXIDATIVE STRESS IN BOVINE EMBRYOS AT THE OOCYTE STAGE IMPROVES BLASTOCIST RATE=> EXPRESSION OF GENES INVOLVED IN THE REGULATION OF CELL REDOX
CARACTERIZACION DE LOS GENES INTS1 Y ZNF516 COMO ONCOGENES Y POTENCIALES BIOMARCADORES PARA LA DETECCION TEMPRANA DEL CANCER CERVICOUTERINO
INTRACYTOPLASMIC SPERM INJECTION IN BOVINE=> EFFECTS OF SPERM TREATMENT AND OOCYTE ACTIVATION ON SUBSEQUENT IN VITRO AND IN VIVO EMBRYO DEVELOPMENT.
OXIDATIVE STRESS IN CRYOPRESERVED BOVINE SPERMATOZOA=> EFFECT ON GENE EXPRESSION AND QUALITY OF EMBRYOS PRODUCED IN VITRO
Efecto de inhibidores de caspasas y proteínas del plasma seminal en la mantención de la función espermática en el proceso de crioprenservación en semen de caninos
Kit de detección de infecciones de transmisión sexual silente en muestras de orina. Una herramienta de diagnóstico no invasiva y de bajo costo

Review (2)

Cryoprotectant-free vitrification of spermatozoa: Fish as a model of human
Preservation of boar semen: An update
31
Raul Sanchez

DIRECTOR DE CENTRO DE EXCELENCIA

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

7
Fabiola Zambrano

Profesor Invitado

UNIVERSIDAD DE LA FRONTERA

TEMUCO, Chile

5
Jennie Risopatrón

Académico y Director del CEBIOR

Ciencias Básicas

Universidad de La Frontera

Temuco, Chile

5
PAMELA URIBE

Docente

Centro de Excelencia en Medicina Traslacional

UNIVERSIDAD DE LA FRONTERA

TEMUCO, Chile

3
felipe pezo

Investigador

Centro de Medicina Traslacional

Universidad de La Frontera

Temuco, Chile

3
Mabel Schulz

Profesor Asistente

Ciencias Preclínicas

Universidad de La Frontera

Temuco, Chile

3
Osvaldo Merino

Investigador

Universidad de La Frontera

Temuco, Chile

1
Pablo Navarro

Docente- Investigador

Departamento Odontología Integral Adulto

Universidad de La Frontera

Temuco, Chile

1
Elías Figueroa

Associate Professor.

Department of Biological and Chemical Sciences

Catholic University of Temuco

Temuco, Chile

1
Alfredo Ramírez

Profesor Asociado

Instituto de Ciencia Animal

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
RICARDO FELMER

Profesor titular

Ciencias Agronomicas y Recursos Naturales

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

1
Jorge Farias

Associate Professor A

Chemical Engineering

Universidad de La Frontera

Temuco, Chile