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Bendek, Maximiliano.; Fitzpatrick, Christopher; Jeldes, Emanuel; boland an; Deleuze, Jean-Francois; Farfan, Nicole; Villegas, Jaime; Montecinos, Martin; Burzio L.O.; Burzio V
Article
Noncoding RNA
(2023)
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Lobos-Gonzalez, L; Oróstica L.; Diaz-Valdivia N.; Rojas-Celis, Victoria; Campos, America; Duran-Jara, Eduardo; Farfan, Nicole; Leyton, Lisette; Quest, Andrew F G
Article
Caveolin-1 (CAV1) is a membrane-bound protein that suppresses tumor development yet also promotes metastasis. E-cadherin is important in CAV1-dependent tumor suppression and prevents CAV1-enhanced lung metastasis. Here, we used murine B16F10 and human A375 melanoma cells with low levels of endogenous CAV1 and E-cadherin to unravel how co-expression of E-cadherin modulates CAV1 function in vitro and in vivo in WT C57BL/6 or Rag−/− immunodeficient mice and how a pro-inflammatory environment generated by treating cells with prostaglandin E2 (PGE2) alters CAV1 function in the presence of E-cadherin. CAV1 expression augmented migration, invasion, and metastasis of melanoma cells, and these effects were abolished via transient co-expression of E-cadherin. Importantly, exposure of cells to PGE2 reverted the effects of E-cadherin expression and increased CAV1 phosphorylation on tyrosine-14 and metastasis. Moreover, PGE2 administration blocked the ability of the CAV1/E-cadherin complex to prevent tumor formation. Therefore, our results support the notion that PGE2 can override the tumor suppressor potential of the E-cadherin/CAV1 complex and that CAV1 released from the complex is phosphorylated on tyrosine-14 and promotes migration/invasion/metastasis. These observations provide direct evidence showing how a pro-inflammatory environment caused here via PGE2 administration can convert a potent tumor suppressor complex into a promoter of malignant cell behavior.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
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Nicole Farfán; Nicole Sanhueza; Macarena Briones; Luis O. Burzio; Verónica A. Burzio
Article
SCIELO
BIOLOGICAL RESEARCH
(2021)
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Fitzpatrick, Christopher; Bendek, Maximiliano F.; Briones, Macarena; Farfan, Nicole; Silva, Valeria A.; Nardocci, Gino; Montecino, Martin; Boland, Anne; Deleuze, Jean-Francois; Villegas, Jaime; Villota, Claudio; Silva, Veronica; Lobos-Gonzalez, Lorena; Borgna, Vincenzo; Barrey, Eric; Burzio, Luis O.; Burzio, Veronica A.
Article
ISI
SCOPUS
CELL DEATH & DISEASE
(2019)
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Varas-Godoy, M; Lladser, A; Farfan, N; Villota, C; Villegas, J; Tapia, JC; Burzio, LO; Burzio, VA; Valenzuela, PDT
Article
ISI
SCOPUS
PIGMENT CELL & MELANOMA RESEARCH
(2018)
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