Man

Nicole Farfán

Asistente de investigación

Universidad del Desarrollo

Santiago, Chile

Líneas de Investigación


Detección de mutaciones fundadoras en Cáncer de mama. RNAs no codificantes y mi RNAs en cáncer

Educación

  •  Biología Molecular, Universidad Andrés Bello. Chile, 2018
  •  Ingeniero en Biotecnologia Molecular, UNIVERSIDAD DE CHILE. Chile, 2010

Experiencia Académica

  •   Profesor adjunto Part Time

    UNIVERSIDAD ANDRES BELLO

    Ciencias de la vida

    Santiago, Chile

    2019 - A la fecha

  •   Profesor adjunto Part Time

    UNIVERSIDAD DE LAS AMERICAS

    Salud y ciencias sociales

    Chile

    2022 - A la fecha

Experiencia Profesional

  •   Postdoc Part Time

    Universidad Católica de Chile

    Chile

    2019 - 2020

  •   Postdoc Full Time

    Universidad de Chile

    Chile

    2018 - 2019

  •   PhD (c) Full Time

    Andes Biotecnologies

    Chile

    2012 - 2018

  •   asistente de investigacion Full Time

    universidad del desarrollo

    santiago, Chile

    2021 - 2022


 

Article (5)

Inverse Modulation of Aurora Kinase A and Topoisomerase IIα in Normal and Tumor Breast Cells upon Knockdown of Mitochondrial ASncmtRNA

boland an; Montecinos, Martin; Burzio L.O.; Burzio V

Article
Noncoding RNA  (2023)

Prostaglandin E2 Exposure Disrupts E-Cadherin/Caveolin-1-Mediated Tumor Suppression to Favor Caveolin-1-Enhanced Migration, Invasion, and Metastasis in Melanoma Models

Leyton, Lisette

Article Caveolin-1 (CAV1) is a membrane-bound protein that suppresses tumor development yet also promotes metastasis. E-cadherin is important in CAV1-dependent tumor suppression and prevents CAV1-enhanced lung metastasis. Here, we used murine B16F10 and human A375 melanoma cells with low levels of endogenous CAV1 and E-cadherin to unravel how co-expression of E-cadherin modulates CAV1 function in vitro and in vivo in WT C57BL/6 or Rag−/− immunodeficient mice and how a pro-inflammatory environment generated by treating cells with prostaglandin E2 (PGE2) alters CAV1 function in the presence of E-cadherin. CAV1 expression augmented migration, invasion, and metastasis of melanoma cells, and these effects were abolished via transient co-expression of E-cadherin. Importantly, exposure of cells to PGE2 reverted the effects of E-cadherin expression and increased CAV1 phosphorylation on tyrosine-14 and metastasis. Moreover, PGE2 administration blocked the ability of the CAV1/E-cadherin complex to prevent tumor formation. Therefore, our results support the notion that PGE2 can override the tumor suppressor potential of the E-cadherin/CAV1 complex and that CAV1 released from the complex is phosphorylated on tyrosine-14 and promotes migration/invasion/metastasis. These observations provide direct evidence showing how a pro-inflammatory environment caused here via PGE2 administration can convert a potent tumor suppressor complex into a promoter of malignant cell behavior.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  (2023)

Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro

Nicole Farfán; Nicole Sanhueza; Macarena Briones; Luis O. Burzio

Article SCIELO
BIOLOGICAL RESEARCH  (2021)

Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors

Bendek, Maximiliano F.; Silva, Valeria A.; Nardocci, Gino; Montecino, Martin; Boland, Anne; Deleuze, Jean-Francois; Burzio, Luis O.

Article ISI SCOPUS
CELL DEATH & DISEASE  (2019)

In vivo knockdown of antisense non-coding mitochondrial RNAs by a lentiviral-encoded shRNA inhibits melanoma tumor growth and lung colonization



Article ISI SCOPUS
PIGMENT CELL & MELANOMA RESEARCH  (2018)

1
Alvaro Lladser

Professor, Principal Investigator

FUNDACIÓN CIENCIA PARA LA VIDA

Santiago, Chile

1
Claudio Villota

Profesor/investigador Asociado

Facultad de Salud

Universidad Bernardo O'Higgins

Santiago, Chile

1
Jaime Villegas

Profesor Asociado

Ciencias Biológicas

Universidad Andrés Bello

Santiago, Chile

1
Gino Nardocci

Investigador / Profesor Asistente

Universidad de Los Andes

Santiago, Chile

1
Martín Montecino

Director

Institute of Biomedical Sciences

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
MANUEL VARAS

Research Professor

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

1
Julio Tapia

Profesor Asociado

Oncologia Basico Clinica

Universidad de Chile

Santiago, Chile

1
Christopher Fitzpatrick

Postoctoral Researcher

Non coding RNAs

Fundación Ciencia y Vida

Santiago, Chile

1
Maximiliano Bendek

ninguno

ninguna

Santiago, Chile

5
Nicole Farfán

Asistente de investigación

instituto de ciencias biomedicas

Universidad del Desarrollo

Santiago, Chile