Man

Roberto Aquiles Zúñiga Olate

Investigador Asociado a Proyectos

Universidad de Chile/ Facultad de Medicina

Santiago, Chile

Líneas de Investigación


Desarrollo de Anticuerpos Terapéuticos para uso en cancer y enfermedades autoinmunes. Desarrollo de promotores de la transcripción para mejorar la expresión y producción de anticuerpos monoclonales recombinantes en células CHO.

Educación

  •  Biologia Molecular, UNIVERSIDAD DE CHILE. Chile, 2003

 

Article (17)

Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells

Zuniga, Roberto A.; Hernan Sotelo, Pablo

Article ISI SCOPUS
JOURNAL OF BIOLOGICAL ENGINEERING  (2019)

Optimization of culture conditions for the expression of three different insoluble proteins in Escherichia coli

Tello, Samantha; Perez-Etcheverry, Diana; Lorenzo-Ferreiro, Carmen

Article ISI SCOPUS
Scientific Reports  (2019)

Transcription factor engineering in CHO cells for recombinant protein production



Article ISI SCOPUS
CRITICAL REVIEWS IN BIOTECHNOLOGY  (2019)

Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNF alpha production in CHO cells

Torres, Mauro

Article ISI SCOPUS
PloS one  (2018)

STAT3 inhibition by STA21 increases cell surface expression of MICB and the release of soluble MICB by gastric adenocarcinoma cells

Garrido-Tapia, Macarena; Hernández, Carolina J.; Ascui, Gabriel; Kramm, Karina; Morales, Marcela; Gárate, Valentina

Article ISI SCOPUS
IMMUNOBIOLOGY  (2017)

Clinical significance of tumor expression of major histocompatibility complex class I-related chains A and B (MICA/B) in gastric cancer patients

Kramm, K.; Galvez-Jiron, F; Pola, V.; Bustamante M.; Contreras, HR; Sabag A.; Garrido-Tapia, M; Hernandez, CJ; Zúñiga R.; Collazo, N.; et al.

Article ISI SCOPUS
ONCOLOGY REPORTS  (2016)

An efficient method for variable region assembly in the construction of scFv phage display libraries using independent strand amplification

Collazo, N.; Zúñiga R.; Gutierrez-Gonzalez M.; Catalan, D; Ribeiro, C.H.; Aguillon, J.C.; Molina, M. C.

Article ISI SCOPUS
mAbs  (2012)

Weak CD4+ T-cell responses to citrullinated vimentin in rheumatoid arthritis patients carrying HLA-DR9 alleles

Catalan, D; Aravena, O; Zúñiga R.; Silva N.; Escobar A.; Sabugo, F; Wurmann, P; Soto L.; Gonzalez R.; Alfaro J.; et al.

Article ISI SCOPUS
Rheumatology International  (2012)

Interleukin 10 decreases MICA expression on melanoma cell surface

Serrano A.E.; Menares-Castillo, E; Garrido-Tapia, M; Ribeiro, C.H.; Gatica-Andrades, M; Valenzuela-Diaz R; Ziga R.; López, M N.; SALAZAR ONFRAY F; Aguillon, J.C.; et al.

Article ISI SCOPUS
Immunology and Cell Biology  (2011)

Modulation of established murine collagen-induced arthritis by a single inoculation of short-term lipopolysaccharide-stimulated dendritic cells

Salazar, L; Aravena, O; Abello P; Escobar A.; Contreras-Levicoy, J; Rojas-Colonelli, N; Catalan, D; Aguirre A.; Zúñiga R.; Pesce B,; et al.

Article ISI SCOPUS
ANNALS OF THE RHEUMATIC DISEASES  (2008)

Short-term lipopolysaccharide stimulation indeces differentiation of murine bone marrow-derived dentritic cells into tolerogenic phenotype

Salazar, L; Aravena, O; Contreras-Levicoy, J; Pesce B,; Catalan, D; Zúñiga R.; Iruretagoyena M.

Article ISI SCOPUS
European Cytokine Network  (2007)

Yeast system as a model to study Moloney murine leukemia virus integrase: expression, mutagenesis and search for eukaryotic partners

Vera J.; Parissi, V; Garcia A.; Zúñiga R.; Andreola, ML; Caumont-Sarcos, A; Tarrago-Litvak, L; Leon, O.

Article ISI SCOPUS
JOURNAL OF GENERAL VIROLOGY  (2005)

Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity

Zúñiga R.; Sengupta, S; Snyder, C.; Leon, O.; Roth, MJ

Article ISI SCOPUS
PROTEIN ENGINEERING DESIGN SELECTION  (2004)

Lysine directed cross-linking of viral DNA-RNA : DNA hybrid substrate to the isolated RNase H domain of HIV-1 reverse transcriptase

Guaitiao, JP; Zuniga, RA; Roth, MJ; Leon, O.

Article ISI SCOPUS
BIOCHEMISTRY  (2004)

A dispensable peptide from Acidithiobacillus ferrooxidans tryptophanyl-tRNA synthetase affects tRNA binding



Article ISI
FEBS LETTERS  (2002)

A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans

Raczniak, G; Becker, H; Soll, D

Article ISI
FEBS LETTERS  (2001)

Conserved amino acids near the carboxy terminus of bacterial tyrosyl-tRNA synthetase are involved in tRNA and Tyr-AMP binding

Lefimil, C; Soll, D

Article ISI
FEBS LETTERS  (2001)

Patent (1)

Promoter with a region rich in cytosine-guanine dinucleotides, vectors, cellular lines, method for producing recombinant protein



Patent
 (2018)

11
MARIA MOLINA

Director

Instituto de Ciencias Biomédicas

Universidad de Chile

INDEPENDENCIA, Chile

1
Lilian Soto

Rheumatologist

Medicine

CLINICAL HOSPITAL OF UNIVERSIDAD DE CHILE

Santiago, Chile

1
Hector Contreras

Academico

Oncologia Basico Clinica

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, INSTITUTO DE CIENCIAS BIOMÉDICAS, PROGRAMA DE FISIOLOGÍA Y BIOFÍSICA

Santiago, Chile

2
Lorena Salazar

Profesora Asistente

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Lampa, Chile

8
JUAN AGUILLON

PROFESSOR

IMMUNOLOGY PROGRAM

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

3
OCTAVIO ARAVENA

Gestión I+D

I+D+i

Comisión Chilena de Energía Nuclear

Santiago, Chile

1
Maria Sabugo

Medico Reumatologo

Medicina

UNIVERSIDAD DE CHILE HOSPITAL CLÍNICO

Santiago, Chile

3
Claudia Altamirano

Profesor Titular

Facultad de Ingeniería

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

Valparaíso, Chile

2
Carolina Ribeiro

Assistant Professor

Immunology Program

UNIVERSIDAD DE CHILE - FACULTAD DE MEDICINA

Santiago, Chile

2
Alejandro Escobar

assistant professor

Institute for Research in Dental Sciences

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Julio Berrios

Académico Full-time

Facultad de Ingeniería

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

1
Antonio Serrano

UNIVERSIDAD CATÓLICA DEL NORTE

Antofagasta, Chile

1
Mercedes López

Profesora Asociada

Instituto de Ciencias Biomédicas

Universidad de chile

Santiago, Chile

1
Marcela Gatica

Académica

Facultad de Ciencias de la Salud

Universidad del Alba

La Serena, Chile

2
Lorena Salazar

Academico

Instituto Cs Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

1
Paula Abello

Docente Adjunto

Ciencias Biológicas

Universidad San Sebastián

Santiago, Chile

1
Jorge Vera

Docente de planta

Universidad Santo Tomás

Puerto Montt, Chile

1
Pamela Wurmann

reumatologa

medicina

universidad de chile

santiago, Chile

4
Norberto Collazo

Business Developer

Center for Sustainable Biotechnology

Fraunhofer Chile Research

Santiago, Chile

5
Matias Gutiérrez

Investigador

Programa Disciplinario de Inmunología

Universidad de Chile

Santiago, Chile

1
LUIS MERCADO

Profesor Titular

Instituto de Biología

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

1
Rodrigo Valenzuela

Bioquímico

Hematologia

Hospital del Salvador

Santiago, Chile

1
Yesenia Latorre

Investigador

Laboratorio cultivo células animales

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

18
Roberto Zúñiga

Investigador Asociado a Proyectos

Programa Disciplinario de Inmunologia

Universidad de Chile/ Facultad de Medicina

Santiago, Chile

1
Mirentxu Iruretagoyena

Jefe Seccion Inmunologia

Laboratorio Clinico

Clinica Alemana de Santiago

Santiago, Chile

6
Diego Catalán

Associate Professor

Programa Disciplinario de Inmunología

Universidad de Chile

Santiago, Chile

2
Bárbara Pesce

Profesora Asistente

ICBM

Universidad de Chile

Santiago, Chile

1
Felipe Galvez

Estudiante Doctorado

Universidad de La Frontera

Temuco, Chile