Man

Yessia Hidalgo Fadic

Flow cytometry unit manager / Researcher

Corporación IMPACT / Universidad de Los Andes

Santiago, Chile

Líneas de Investigación


Autoimmunity, aging and inflammation process, and how inducing functional changes in the immune cells. Mitochondria, intracellular and extracellular in autoimmunity. Extracellular vesicles. Flow Cytometry expert.

Educación

  •  Doctorado en Ciencias con mención en Biología Molecular, Celular y Neurociencias, UNIVERSIDAD DE CHILE. Chile, 2018
  •  Ingeniero en Biotecnología Molecular, UNIVERSIDAD DE CHILE. Chile, 2012
  •  Licenciado en Biotecnología Molecular, UNIVERSIDAD DE CHILE. Chile, 2011

Experiencia Profesional

  •   PhD student Full Time

    Universidad de Chile

    Santiago, Chile

    2013 - 2017

  •   Research Assistant Full Time

    Universidad de Chile

    Santiago, Chile

    2011 - 2012

  •   Undergraduate student Full Time

    Universidad de Chile

    Santiago, Chile

    2010 - 2011

  •   Research Assistant Full Time

    Fundacion Ciencia y Vida

    Santiago, Chile

    2017 - 2018

  •   Insternship Other

    Curie Institute

    Paris, Francia

    2015 - 2015

  •   Postdoctoral researcher Part Time

    Cells for Cells

    Santiago, Chile

    2018 - 2020

  •   Flow facility manager Part Time

    Consorcio Regenero

    Santiago, Chile

    2019 - 2021

  •   Young researcher Part Time

    Cells for Cells

    Santiago, Chile

    2021 - 2020

  •   Researcher Part Time

    Universidad de Los Andes

    Santiago, Chile

    2022 - At present

  •   Flow Cytometry Facility Manager Part Time

    Corporación IMPACT

    Santiago, Chile

    2022 - At present

  •   Investigador principal Fondecyt Iniciación 11221017 Part Time

    Universidad de Los Andes

    Chile

    2022 - 2025

Formación de Capital Humano


Flow Cytometry expert.
During my researcher career, I acquired strong knowledge in the flow cytometry technique, mainly in the Maria Rosa Bono's lab. This knowledge allowed me to found work as Flow Cytometry Facility Manager of Cells for Cells/Regenero institution. Currently, this experience help me in the design, performing and analysis of the experiments. Currently I work as Flow Cytometry Facility Manager of Centro de Investigación e Innovación Biomédica (CIIB), Universidad de Los Andes, Santiago, Chile.

Supervision undergraduate students.
I had a student in charge. This student was María José Fuenzalida, an undergraduate student of the engineer in molecular biotechnology Career of University of Chile. During the years 2014 to 2016, She developed an internship and her Title Seminar in the laboratory. I trained her in terms related to design the experimental proposal and taught her the experimental techniques to perform her proposal. The work that developed María José Fuenzalida is titled “Estudio de las subpoblaciones de linfocitos B y T presentes en el timo de ratones que desarrollan lupus”

Currently, I am a tutor of Lucas Cereceda, that he performs its undergraduate thesis to Biotechnology career from Universidad Mayor. Lucas characterizes circulating mitochondria in plasma physically and functionally in healthy donors and lupus patients. He will finish his thesis in the next two months.

Also I have a student Gabriela Maita from Biology Career of Pontificia Universidad Católica de Santiago. She performs her professional practice to finish his career.

Teaching activities.
I perform classroom lessons in the field of immunology. At the University of Chile, Faculty of Sciences is taught the course the Basic Immunology to the Engineer of Molecular Biotechnology career, led by Ph.D. Maria Rosa Bono. In this course, the main foundations of immunology are taught. I am part of teaching the item how the immune system behaves when it responds in an exaggerated manner, such as in hypersensitivity reactions: allergies and autoimmunity. I have taught this knowledge since 2014.

I perform class lessons in the Doctoral Biomedicine Program of Universidad de Los Andes, Santiago, Chile. These classes are related to the flow cytometry technique and its applications. Also I perform practical activities in flow cytometry with the students.


Premios y Distinciones

  •   Yessia Hidalgo

    UNIVERSIDAD DE CHILE

    Chile, 2013

    CONICYT Doctoral Scholarship Beca Doctorado Nacional CONICYT # 21130598


 

Article (22)

Clinical-grade extracellular vesicles derived from umbilical cord mesenchymal stromal cells: preclinical development and first-in-human intra-articular validation as therapeutics for knee osteoarthritis

Georges-Calderon, Nicolas; Tobar, Hugo E.; Cuenca, Jimena; Varela, Cristobal; Vernal, Rolando; Nardocci, Gino; Adasme-Vidal, Catalina; Kunze-Kullmer, Maximiliano; Hidalgo, Yessia

Article ISI
JOURNAL OF NANOBIOTECHNOLOGY  (2025)

Mitochondrial Antiviral Signaling Protein Activation by Retinoic Acid-Inducible Gene I Agonist Triggers Potent Antiviral Defense in Umbilical Cord Mesenchymal Stromal Cells Without Compromising Mitochondrial Function

Castillo-Galan, Sebastian; Grunenwald, Felipe; Hidalgo, Yessia; Cardenas, J. Cesar; Cadiz, Maria Ignacia; Alcayaga-Miranda, Francisca; Khoury, Maroun; Cuenca, Jimena

Article ISI
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES  (2025)

Safety and efficacy of mesenchymal stromal cells mitochondria transplantation as a cell-free therapy for osteoarthritis

Garcia-Guerrero, Cynthia; Yanten-Fuentes, Liliana; Jeraldo, Nicole; Aros, Constanza; Troncoso, Francisca; Almarza, Gonzalo; Toledo, Jorge; Oyarce, Karina; Vernal, Rolando; Hidalgo, Yessia

Article ISI
JOURNAL OF TRANSLATIONAL MEDICINE  (2025)

A Phase I Dose-Escalation Clinical Trial to Assess the Safety and Efficacy of Umbilical Cord-Derived Mesenchymal Stromal Cells in Knee Osteoarthritis

Vernal, Rolando; Hidalgo, Yessia; Cuenca, Jimena

Article ISI
STEM CELLS TRANSLATIONAL MEDICINE  (2024)

Impact of platelet-derived mitochondria transfer in the metabolic profiling and progression of metastatic MDA-MB-231 human triple-negative breast cancer cells

Cereceda, Lucas; Hidalgo, Yessia

Article ISI
Frontiers in Cell and Developmental Biology  (2024)

Isolation of Intestinal Macrophage Subpopulations for High-Quality Total RNA Purification in Zebrafish

Del Rio-Jay, Yalen; Duperray, Christophe; Hidalgo, Yessia

Article ISI
METHODS AND PROTOCOLS  (2024)

Levels of Small Extracellular Vesicles Containing hERG-1 and Hsp47 as Potential Biomarkers for Cardiovascular Diseases

Osorio, Luis A.; Moreno-Hidalgo, Viviana; Arevalo-Gil, Angely; Ramirez-Balaguera, Angie; Hevia, Daniel; Cifuentes, Jorge; Hidalgo, Yessia; Urquidi, Cinthya; Iturriaga, A; Rivera-Palma, Alejandra; et al.

Article
 (2024)

Mitochondrial division inhibitor (mdivi-1) induces extracellular matrix (ECM)-detachment of viable breast cancer cells by a DRP1-independent mechanism

Morgado-Caceres, Pablo; Kangme-Encalada, Matias; de Arbina, Amaia Lopez; Hidalgo, Yessia

Article ISI
SCIENTIFIC REPORTS  (2024)

Lactate: an alternative pathway for the immunosuppressive properties of mesenchymal stem/stromal cells

Oyarce, Karina; Bustos, Andres; Lagos, Raul; Abba Moussa, Daouda; Hidalgo-Fadic, Yessia

Article ISI
STEM CELL RESEARCH & THERAPY  (2023)

Safety and efficacy of clinical-grade, cryopreserved menstrual blood mesenchymal stromal cells in experimental acute respiratory distress syndrome

Silva, Johnatas Dutra; da Silva, Luisa Helena Andrade; Cruz, Fernanda Ferreira; Utreras, Yildy; Hidalgo, Yessia; Cadiz, Maria Ignacia; Limonchi, Rafael Tapia; Cuenca, Jimena

Article ISI
Frontiers in Cell and Developmental Biology  (2023)

Mesenchymal stem cell-mediated transfer of mitochondria: mechanisms and functional impact

Hidalgo, Yessia

Article ISI
CELLULAR AND MOLECULAR LIFE SCIENCES  (2022)

A Chemically Defined, Xeno- and Blood-Free Culture Medium Sustains Increased Production of Small Extracellular Vesicles From Mesenchymal Stem Cells

Hidalgo, Yessia

Article ISI
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY  (2021)

The Multifaceted Roles of B Cells in the Thymus: From Immune Tolerance to Autoimmunity

Castaneda, Justine; Hidalgo, Yessia; Sauma, Daniela; Rosemblatt, Mario; Bono, Maria Rosa

Article ISI
FRONTIERS IN IMMUNOLOGY  (2021)

Cells from discarded dressings differentiate chronic from acute wounds in patients with Epidermolysis Bullosa

Fuentes, Ignacia; Guttmann-Gruber, Christina; Tockner, Birgit; Diem, Anja; Cofre-Araneda, Glenda; Figuera, Olga; Hidalgo, Yessia; Palisson, Francis; Joao Yubero, Maria; Cho, Raymond J.; et al.

Article ISI
SCIENTIFIC REPORTS  (2020)

Thymic B Cells Promote Germinal Center-Like Structures and the Expansion of Follicular Helper T Cells in Lupus-Prone Mice

Hidalgo, Yessia; Jose Fuenzalidal, Maria; Martinez, Victor; Rosemblatt, Mario; Sauma, Daniela

Article ISI
FRONTIERS IN IMMUNOLOGY  (2020)

Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response

Sauma, Daniela; Hidalgo, Yessia; Rosemblatt, Mario

Article ISI SCOPUS
IMMUNOLOGIC RESEARCH  (2017)

Purinergic Signaling as a Regulator of Th17 Cell Plasticity

Dominique Fernandez, Felipe Flores-Santibañez; Jocelyn Neira, Francisco Osorio-Barrios; Gabriela Tejón, Sarah Núñez; Yessia Hidalgo, María José Fuenzalida; Daniel Meza, Gonzalo Ureta; Alvaro Lladser, Rodrigo Pacheco ; Claudio Acuña-Castillo, Victoria Guixé; Francisco J. Quintana, María Rosa Bono

Article
Plos One  (2016)

The human thymus perivascular space is a functional niche for viral-specific plasma cells

Moore, Carolina; Hidalgo, Yessia

Article ISI
SCIENCE IMMUNOLOGY  (2016)

Alloreactive Regulatory T Cells Allow the Generation of Mixed Chimerism and Transplant Tolerance

Ruiz, Paulina; Maldonado, Paula; Hidalgo, Yessia; Sauma, Daniela; Rosemblatt, Mario; Bono, Maria Rosa

Article ISI SCOPUS
Frontiers in immunology  (2015)

Alloreactive regulatory T cells generated with retinoic acid prevent skin allograft rejection

Moore, C; Tejon, G.; Fuentes, C; Hidalgo, Y; Bono, MR; Maldonado P.; Fernández, R; Wood K.J.; Fierro J.A.; Rosemblatt, M; et al.

Article ISI SCOPUS
EUROPEAN JOURNAL OF IMMUNOLOGY  (2015)

Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation

Tejn, Gabriela; Manrquez, Valeria; De Calisto, Jaime; Flores-Santibez, Felipe; Hidalgo, Yessia; Crisstomo, Natalia; Fernndez, Dominique; Sauma, Daniela; Mora, J. Rodrigo; Bono, Mara R.; et al.

Article ISI SCOPUS
BioMed Research International  (2015)

T helper type 17 cells contribute to anti-tumour immunity and promote the recruitment of T helper type 1 cells to the tumour

Nuñez, S; Saez, JJ; Fernández, D; Flores-Santibanez, F; Alvarez, K; Tejon, G.; Ruiz, P; Maldonado P.; Hidalgo, Y; Manriquez, V.; et al.

Article ISI SCOPUS
Immunology  (2013)

Abstract (4)

CHARACTERIZATION OF MESENCHYMAL STROMAL CELLS AND THEIR MITOCHONDRIA IN RESPONSE TO RIG-I AGONIST

Grunenwald, F.; Castillo-Galan, S; Cardenas, C; Hidalgo, Y; Munoz, D; Alcayaga-Miranda, F; Khoury, M; Cuenca J.

Abstract ISI
CYTOTHERAPY  (2024)

EFFECTS OF MITOCHONDRIAL TRANSPLANTATION ON INTRACELLULAR ANTIVIRAL PATHWAYS, RESTORATIVE ACTIONS, AND IMMUNOMODULATORY RESPONSES IN RIG-I AGONIST-ACTIVATED LUNG CELLS

Castillo-Galan, S; Grunenwald, F.; Cardenas, C; Hidalgo, Y; Rodriguez, F.; Inostroza T.; Alcayaga-Miranda, F; Khoury, M; Cuenca J.

Abstract ISI
CYTOTHERAPY  (2024)

Accumulation of autoreactive antibody-producing cells in the thymus of lupus-prone BWF1 mice

Hidalgo, Y

Abstract ISI
EUROPEAN JOURNAL OF IMMUNOLOGY  (2019)

Allogeneic inducible regulatory T cells allow the generation of mixed chimerism via nonmyeloablative conditioning in mice models

Ruiz, P; Maldonado P.; Hidalgo, Y; Silva, C; Ramírez, V; Rosemblatt, M; Bono, M.

Abstract ISI
JOURNAL OF IMMUNOLOGY  (2012)

BookSection (1)

A Spontaneous Mouse Model of Lupus: Physiology and Therapy

Hidalgo, Yessia; Bono, Maria Rosa; Rosemblatt, Mario

BookSection
 (2020)

Proyecto (3)

REVEALING THE CONTRIBUTION OF MELANOMA-DERIVED CIRCULATING CELL-FREE MITOCHONDRIA IN THE FORMATION OF PREMETASTATIC NICHE IN LYMPH NODES AND THEIR IMPACT IN THE EARLY DEVELOPMENT OF METASTASIS

Alcayaga, Francisca

Proyecto
 (2023)

INVESTIGATING THE IMMUNOREGULATORY EFFECT OF CIRCULATING CELL-FREE MITOCHONDRIA FROM HEALTHY DONORS AND SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS ON MEMORY CD4+ T CELLS.

Hidalgo, Yessia

Proyecto
 (2022)

INVESTIGATING THE EFFECT OF MAVS-ENRICHED MITOCHONDRIAL TRANSPLANTATION ON INTRACELLULAR ANTIVIRAL IMMUNITY AND THEIR RESTORATIVE AND IMMUNOMODULATORY EFFECTS IN A VIRAL-INDUCED ACUTE LUNG INJURY MODEL.

Cuenca, Jimena; Hidalgo, Yessia

Proyecto
 (2021)

Review (1)

Transplant Tolerance: New Insights and Strategies for Long-Term Allograft Acceptance

Ruiz, P; Maldonado P.; Hidalgo, Y; gleisner a; Sauma, D; Silva, C; Saez, JJ; Nuñez, S; Rosemblatt, M; Bono, AR

Review ISI SCOPUS
Clinical and Developmental Immunology  (2013)

10
Maroun Khoury

Professor

Faculty of Medicine

Universidad de Los Andes

santiago, Chile

9
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Ricardo Fernández

Associate Professor

Salud

Universidad de Los Lagos

Osorno, Chile

1
Claudia Altamirano

Profesor Titular

Facultad de Ingeniería

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

Valparaíso, Chile

2
Rolando Vernal

Profesor Titular

Departamento de Odontología Conservadora

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Jimena Cuenca

Investigador/profesor

Facultad de Medicina

Universidad de Los Andes

Santiago, Chile

1
Carmen Feijóo

Profesor asociado

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Alejandro Bruhn

Profesor Titular. Director de Investigación y Postgrado, Facultad de Medicina

Departamento de Medicina Intensiva

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

3
Francisca Alcayaga

Profesor Asociado

Facultad de Medicina

UNIVERSIDAD DE LOS ANDES

Santiago, Chile

1
Hugo Tobar

GLP Unit Manager

IMPACT

IMPACT Center, Universidad de los Andes

Santiago, Chile

3
Julio Cardenas

Associated Professor

Center for Integrative Biology

Universidad Mayor de Chile

Santiago, Chile

6
Daniela Sauma

Academic and researcher

Biologia

Universidad de Chile

Santiago, Chile

1
Andrea del Campo

Profesor Asistente, Investigador Principal

Departamento de Farmacia

Pontificia Universidad Católica de Chile

Santiago, Chile

8
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

3
Gabriela Tejon

Investigador Postdoctoral

Biología

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

5
Patricia Luz

Profesor asistente

Universidad de los Andes

Santiago , Chile

4
Paulina Ruiz

Profesor Asistente

UNIVERSIDAD DE CHILE

Santiago, Chile

5
Sarah Nuñez

Académico Investigador

Facultad de Ciencias de la Salud

Universidad San Sebastián

Santiago, Chile

1
Jorge Toledo

Investigador

Centro de Investigacion Clinica Avanzada CICA

Universidad de Chile, Hospital Clinico Universidad de Chile

Santiago, Chile

2
Karina Oyarce

Profesora asistente - Investigadora

Facultad de Medicina y Ciencia

Universidad San Sebastián, sede Tres Pascualas

Concepción, Chile

1
Roberto Elizondo

Profesor Asistente

Departamento de Biología Celular

Universidad de Concepción

CONCEPCION, Chile

1
Boris Rebolledo

Assistant Professor

Center for Genetics and Genomics

Universidad del Desarrollo

Santiago, Chile

2
María Gleisner

INVESTIGADORA

ICBM

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Ricardo Fernandez

Profesor Asociado A

Salud

Universidad de Los Lagos

Osorno, Chile

1
Jaime De Calisto

Director Docente

Escuela de Biotecnología

Universidad Mayor

Santiago, Chile

2
Aliosha Figueroa

Asistente de Investigación Senior

Centro de Medicina Intervencional de Precisión y Terapia Celular Avanzada (IMPACT)

Santiago, Chile

30
Yessia Hidalgo

Flow cytometry unit manager / Researcher

Centro de Investigación e Innovación Biomédica

Corporación IMPACT / Universidad de Los Andes

Santiago, Chile

1
Charlotte Hill

Postdoctoral Fellow

CIIB

Universidad de Los Andes

Santiago, Chile

2
RADMILA VELARDE

PostDoc

Escuela de Medicina

Universidad de Los Andes

Santiago, Chile

1
Sebastian Castillo

Postdoc

Medicina

Universidad de Los Andes

Santiago, Chile

2
Camila Fuentes

Investigador Postdoctoral

Facultad de Medicina

Universidad de Los Andes

Santiago, Chile

1
Nicole Jeraldo

1
Felipe Galvez

Estudiante Doctorado

Universidad de La Frontera

Temuco, Chile