Man

Victoria Cristina Guixé Leguía

Titular Profesor

UNIVERSIDAD DE CHILE

Santiago, Chile

Líneas de Investigación


Evolutionary trajectories of molecular mechanisms underlying enzyme adaptations to extreme environments. Unveiling the allosteric regulation of central carbon metabolism in Archaea through kinetic, structural, and evolutive studies of key enzymes.

Educación

  •  Biological Science, UNIVERSIDAD DE CHILE. Chile, 1985
  •  Bachelors degree in Biology, UNIVERSIDAD DE CHILE. Chile, 1978

Experiencia Académica

  •   Profesor Titular Full Time

    UNIVERSIDAD DE CHILE

    Ciencias

    Santiago, Chile

    1986 - At present

  •   Instructor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Ciencias Biológicas

    Santiago, Chile

    1985 - 1986

Experiencia Profesional

  •   Profesor Titular Full Time

    Universidad de Chile

    Santiago, Chile

    1986 - At present

Formación de Capital Humano


Posdoctorado
• Consuelo Badilla Posdoctorado Conicyt, Historia evolutiva de la especificidad de unión de oxianiones de arsénico en la subfamilia de proteínas periplasmáticas mediante la resurrección de proteínas ancestrales (en curso).
• Alejandra Herrera Morandé Caracterización funcional y estructural de la glucoquinasa humana dependiente de ADP Posdoctorado Conicyt (2019)

Doctorado
• Ricardo Cabrera, Doctor en Ciencias, Programa de Biología Molecular, Celular y Neurociencia, Facultad de Ciencias, Universidad de Chile. Control alostérico de la oligomerización y la inhibición de la actividad catalítica en la fosfofructoquinasa-2 de E. coli. Consecuencias estructurales de la unión de ligandos. (2004).
• Mauricio Báez, Doctorado en Bioquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile. Importancia de la interfaz monómero-monómero y dímero-dímero en la estabilidad y regulación alostérica de la fosfofructoquinasa-2 de Escherichia coli. Codirectora (2008).
• Jaime Andrés Rivas, Doctorado en Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Cambios conformacionales, estabilidad mecánica y determinantes moleculares de la especificidad por ligando de la glucoquinasa dependiente de ADP de Thermococcus litoralis. (enero 2012).
• Diego Quiroga Roger, Doctorado en Bioquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile. Catálisis y regulación de la riboquinasa humana. Papel del motivo NXXE en la unión de PO4-3 y Mg?2 (2014).
• César Ramírez, Doctorado en Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Importancia de la topología de plegamiento en la estabilidad conformacional y las propiedades de la interfaz de asociación en miembros de la superfamilia riboquinasa. (2013).
• Lorena Pizarro, Doctorado en Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Identificación y Estudio Funcional de Factores de Transcripción que Regulan la Expresión de Genes del Sistema de Endomembranas en Arabidopsis thaliana, Codirectora (2015).
• Víctor Castro, Doctorado en Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Resurrección de enzimas ancestrales: historia evolutiva de la especificidad por sustratos en la familia de quinasas de azúcares dependientes de ADP de arqueas (2015).
• Alejandra Herrera-Morandé, Programa de Doctorado en Biotecnología, mención Genómica, Proteómica y Bioinformática, Universidad de Barcelona, Biología estructural de enzimas relacionadas con el metabolismo de glucosa y bases púricas. Codirectora en conjunto con la Dra. Vega (2015).
• Ricardo Zamora, Doctorado en Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile Bases moleculares de la adaptación al frío en enzimas: Estudio comparativo entre homólogos extremófilos de la familia de quinasas de azúcares dependientes de ADP de arqueas (2016).
• Gabriel Vallejos Doctorado en Biología Molecular, Celular y Neurociencias. Mecanismo y evolución de la regulación alostérica de quinasas de azúcares dependientes de ADP de Arqueas. (2020).
• Felipe González-Ordenes Doctorado en Biología Molecular, Celular y Neurociencias. Regulación alostérica por AMP en enzimas del metabolismo central de arqueas. (en curso).
• Claudia Mella Hernández, Co-Directora de Tesis de Doctorado en Biología Molecular, Celular y Neurociencias. "Estudio de la historia evolutiva de inserciones y/o deleciones en enzimas pertenecientes a la familia Histidina Ácido Fosfatasas y su impacto sobre la flexibilidad y especificidad por sustrato".

Magíster
• Gabriel Campos, Tesis para optar al grado de Magister en Ciencias Biológicas con Mención en Bioquímica. Facultad de Ciencias, Universidad de Chile. Mecanismo cinético de fosfofructoquinasas tipo 2 de Escherichia coli. (1984).
• Fernando Olate Tesis para optar al título de Bioquímico y grado de Magíster en Bioquímica, Universidad Andrés bello. Análisis funcional y estructural de los sitios de unión de ligandos en quinasas dependiente de ADP del dominio Archaea (Thermococcus litoralis y Pyrococcus furiosus) (2009).
• César Ramírez, Magíster Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Determinantes estructurales del sitio activo de la piridoxal quinasa humana. Actividad catalítica e interacción con cationes (2010).
• Felipe Merino, Magíster Biología Molecular, Celular y Neurociencias, Facultad de Ciencias, Universidad de Chile. Evolución de la especificidad por nucleótidos en la superfamilia riboquinasa: Influencia de la temperatura en la discriminación ATP/ADP en quinasas dependientes de ADP (2011).
• Magaly León, Tesis para optar al título de Bioquímico y grado de Magíster en Bioquímica, Universidad Andres Bello. Determinantes estructurales del sitio de unión a nucleótido en riboquinasa de Homo sapiens (2012).
• Felipe González Tesis para optar al título de Bioquímico y grado de Magíster en Bioquímica, Universidad de Chile. Evolución de la halofilicidad en fosfofructoquinasas dependientes de ADP del orden Methanosarcinales de arqueas (2017).
• Amparo Nuñez. Tesis para optar al título de Bioquímico y grado de Magíster en Bioquímica, Universidad de Chile Determinación de la estrategia de haloadaptación de una quinasa dependiente de ADP de Nanohaloarquea (2020).
• Sebastián Muñoz Magister en Ciencias Biológicas, Facultad de Ciencias, U de Chile. Estudios estructurales de la hemocianina de Concholepas concholepas (2023).
• Nicolás Fuentes, Co-Directora Tesis Magíster en Ciencias Biológicas. HISTORIA EVOLUTIVA DE LA ACTIVIDAD 4-AMINO-5-HIDROXIMETIL-2-METILPIRIMIDINA FOSFATO QUINASA DE BACTERIAS (2023).
• Charlotte Palominos Magister en Ciencias Biológicas, Facultad de Ciencias, U de Chile. La disfunción mitocondrial producida por inhibición farmacológica de la 2-oxoglutarato deshidrogenasa (OGDH) induce muerte celular selectiva de células madre tumorales de pulmón bajo condiciones hipóxicas (2023).
• Ana Belén Tenorio Ricca Magister en Ciencias Biológicas, Facultad de Ciencias, U de Chile. La toxina Asp49-svPLA2 de la serpiente Bothrops pictus (Tschudi, 1845) produce disfunción mitocondrial, reduciendo la viabilidad y migración de la línea celular de cáncer de mama triple negativo MDA-MB-231 (en curso).

Pregrado
• Andrés Caniuguir, Bioquímico, Universidad Austral de Chile. Análisis del rol estructural y funcional de la cisteína 238 de la fosfofructoquinasa-2 de E. coli por mutagénesis sitio dirigida. (2004)
• Rafael Parducci, Ingeniería en Biotecnología Molecular, Universidad de Chile. Importancia de los residuos Aspártico 256 y Glutámico 190 en la catálisis y unión de los sustratos en Pfk-2 de E. coli. (2005)
• Freddy Navarro, Bioquímico, Universidad de Chile. Análisis estructural, evolutivo y funcional de la piridoxal quinasa de humano. (2010)
• Ivonne García, Ingeniería en Biotecnología Molecular, Universidad de Chile. Efecto de cationes metálicos divalentes sobre la catálisis de la glucoquinasa ADP-dependiente de Pyrococcus furiosus. Papel del motivo conservado NXXE. (2011)
• Felipe Merino León, Biólogo con mención en medio ambiente, Universidad de Chile Adaptaciones de arqueas hipertermófilas a ambientes de altas temperaturas: estudios de estabilidad térmica y mecanismo cinético en quinasas dependientes de ADP. (2011).
• Felipe Bravo Moraga, Ingeniería en Bioinformática, U de Talca. Historia evolutiva de la actividad bifuncional hidroximetilpirimidina quinasa / piridoxal quinasa (2013).
• Evelin González, Ingeniería en Bioinformática, U de Talca. Historia evolutiva de la actividad piridoxal quinasa en el grupo de las hidroximetil/pirimidina quinasas de bacterias (2015).
• Macarena Kern M. Ingeniería en Biotecnología Molecular, Universidad de Chile. Estudio de la flexibilidad de las ADP-PFK/GK de M. burtonii y M. maripaludis como mecanismo de adaptación al frío en proteínas (2016).
• Pablo Cea Ingeniería en Biotecnología Molecular, Facultad de Ciencias. Caracterización bioquímica de 4-amino-5-hidroximetil-2-metilpirimidina quinasa de Salmonella typhimurium y Thermus thermophilus (2017).
• Nicolás Fuentes Ingeniería en Biotecnología Molecular, Facultad de Ciencias. Caracterización de la glucosa-6-fosfato deshidrogenasa de la haloarquea Haloferax volcanii para el diseño de un ensayo acoplado de la actividad glucoquinasa de enzimas halófilas (2020).
• Sebastián Muñoz Ingeniería en Biotecnología Molecular, Facultad de Ciencias. Estructura cristalina del complejo ternario de una fosfofructoquinasa dependiente de ADP ancestral y mutagénesis de su sitio activo (2020).


Difusión y Transferencia


Participación en iniciativa Explora, Conicyt.
Curso electivo para estudiantes de 2 ° medio del Liceo Manuel de Salas.
Difusión del quehacer científico a través de Instagram y Facebook (Laboratorio de Bioquímica y Biología Molecular de Chile)


Premios y Distinciones

  •   Premio mejor docente

    UNIVERSIDAD DE CHILE

    Chile, 2005

    Premio mejor docente 2005

  •   Premio Mujer Generación Siglo XXI

    UNIVERSIDAD DE CHILE

    Chile, 2009

    Premio Mujer Generación Siglo XXI 2009


 

Article (103)

Structural insights into a functional unit from an immunogenic mollusk hemocyanin
Kinetic characterization and phylogenetic analysis of human ADP-dependent glucokinase reveal new insights into its regulatory properties
Antarctic Polyester Hydrolases Degrade Aliphatic and Aromatic Polyesters at Moderate Temperatures
Characterisation of kinetics, substrate inhibition and product activation by AMP of bifunctional ADP-dependent glucokinase/phosphofructokinase from Methanococcus maripaludis
The Emergence of New Catalytic Abilities in an Endoxylanase from Family GH10 by Removing an Intrinsically Disordered Region
Crystal structure and molecular dynamics simulations of a promiscuous ancestor reveal residues and an epistatic interaction involved in substrate binding and catalysis in the ATP-dependent vitamin kinase family members
Structure of an ancestral ADP-dependent kinase with fructose-6P reveals key residues for binding, catalysis, and ligand-induced conformational changes
An Evolutionary Marker of the Ribokinase Superfamily Is Responsible for Zinc-Mediated Regulation of Human Pyridoxal Kinase
Characterization of hydroxymethylpyrimidine phosphate kinase from mesophilic and thermophilic bacteria and structural insights into their differential thermal stability
Free radicals derived from γ-radiolysis of water and AAPH thermolysis mediate oxidative crosslinking of eGFP involving Tyr-Tyr and Tyr-Cys bonds: the fluorescence of the protein is conserved only towards peroxyl radicals
Tuning of Conformational Dynamics Through Evolution-Based Design Modulates the Catalytic Adaptability of an Extremophilic Kinase
ADP-Dependent Kinases From the Archaeal Order Methanosarcinales Adapt to Salt by a Non-canonical Evolutionarily Conserved Strategy
ADP-dependent kinases from the archaeal order Methanosarcinales adapt to salt by a non-canonical evolutionary conserved strategy
Protein topology determines substrate-binding mechanism in homologous enzymes
ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activity
ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activity
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases (vol 292, pg 15598, 2017)
Purinergic Signaling as a Regulator of Th17 Cell Plasticity
Purinergic Signaling as a Regulator of Th17 Cell Plasticity
Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency
Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.
CD73-mediated adenosine production promotes stem cell-like properties in mouse Tc17 cells.
Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase from Thermococcus litoralis
Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase from Thermococcus litoralis
Identifying Sequential Substrate Binding at the Single-Molecule Level by Enzyme Mechanical Stabilization
Identifying Sequential Substrate Binding at the Single-Molecule Level by Enzyme Mechanical Stabilization
Role of monovalent and divalent metal cations in human ribokinase catalysis and regulation
Role of monovalent and divalent metal cations in human ribokinase catalysis and regulation
The Folding Unit of Phosphofructokinase-2 as Defined by the Biophysical Properties of a Monomeric Mutant
The Folding Unit of Phosphofructokinase-2 as Defined by the Biophysical Properties of a Monomeric Mutant
Bifunctional ADP-dependent phosphofructokinase/ glucokinase activity in the order Methanococcales biochemical characterization of the mesophilic enzyme from Methanococcus maripaludis
Bifunctional ADP-dependent Phosphofructokinase/Glucokinase activity in the order Methanococcales: Biochemical characterization of the mesophilic enzyme from Methanococcus maripaludis
Emergence of pyridoxal phosphorylation through a promiscuous ancestor during the evolution of hydroxymethyl pyrimidine kinases
Emergence of pyridoxal phosphorylation through a promiscuous ancestor during the evolution of hydroxymethyl pyrimidine kinases
Emergence of pyridoxal phosphorylation through a promiscuous ancestor during the evolution of hydroxymethyl pyrimidine kinases.
A Ribokinase Family Conserved Monovalent Cation Binding Site Enhances the MgATP-induced Inhibition in E. coli Phosphofructokinase-2
A ribokinase family conserved monovalent cation binding site enhances the MgATP-induced inhibition in E. coli phosphofructokinase-2.
Catalytic and regulatory roles of species involved in metal-nucleotide equilibriums in human pyridoxal kinase
Catalytic and regulatory roles of species involved in metal-nucleotide equilibriums in human pyridoxal kinase
Crystal structure, SAXS and kinetic mechanism of hyperthermophilic ADP-dependent glucokinase from Thermococcus litoralis reveal a conserved mechanism for catalysis
Crystal Structure, SAXS and Kinetic Mechanism of Hyperthermophilic ADP-Dependent Glucokinase from Thermococcus litoralis Reveal a Conserved Mechanism for Catalysis
Observation of Solvent Penetration during Cold Denaturation of E. coli Phosphofructokinase-2
Observation of solvent penetration during cold denaturation of E. coli phosphofructokinase-2
Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaea
Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaea
Expanded Monomeric Intermediate upon Cold and Heat Unfolding of Phosphofructokinase-2 from Escherichia coli
Expanded Monomeric Intermediate upon Cold and Heat Unfolding of Phosphofructokinase-2 from Escherichia coli.
Divalent metal cation requirements of phosphofructokinase-2 from E. coli. Evidence for a high affinity binding site for Mn 2+
Divalent metal cation requirements of phosphofructokinase-2 from E. coli. Evidence for a high affinity binding site for Mn2+
Divalent metal cation requirements of phosphofructokinase-2 from E. coli. Evidence for a high affinity binding site for Mn2+
Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coli
Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coli
ADP-dependent 6-Phosphofructokinase from Pyrococcus horikoshii OT3 STRUCTURE DETERMINATION AND BIOCHEMICAL CHARACTERIZATION OF PH1645
ADP-dependent 6-Phosphofructokinase from Pyrococcus horikoshii OT3 STRUCTURE DETERMINATION AND BIOCHEMICAL CHARACTERIZATION OF PH1645
Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability
The ADP-dependent Sugar Kinase Family: Kinetic and Evolutionary Aspects
Crystallographic Structure of Phosphofructokinase-2 from Escherichia coli in Complex with Two ATP Molecules. Implications for Substrate Inhibition
Crystallographic Structure of Phosphofructokinase-2 from Escherichia coli in Complex with Two ATP Molecules. Implications for Substrate Inhibition
Specificity evolution of the ADP-dependent sugar kinase family - in silico studies of the glucokinase/phosphofructokinase bifunctional enzyme from Methanocaldococcus jannaschii
Specificity evolution of the ADP-dependent sugar kinase family - in silico studies of the glucokinase/phosphofructokinase bifunctional enzyme from Methanocaldococcus jannaschii
Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli
Unfolding pathway of the dimeric and tetrameric forms of phosphofructokinase-2 from Escherichia coli.
Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase family
Crystallization and preliminary crystallographic analysis of the tetrameric form of phosphofructokinase-2 from Escherichia coli, a member of the ribokinase family.
Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: Regulatory properties of a ribokinase family member
Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase family member.
Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli
Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli.
Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle X-ray scattering and homology modeling
Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle x-ray scattering and homology modeling.
Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2
Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2.
Ligand-dependent structural changes and limited proteolysis of Escherichia coli phosphofructokinase-2
Ligand-dependent structural changes and limited proteolysis of Escherichia coli phosphofructokinase-2.
Chemical modification of SH groups of E-coli phosphofructokinase-2 induces subunit dissociation: Monomers are inactive but preserve ligand binding properties
Chemical modification of SH groups of E. coli phosphofructokinase-2 induces subunit dissociation: monomers are inactive but preserve ligand binding properties.
Physiological consequences of a non-regulated mutant phosphofructokinase in Escherichia coli
In vivo operation of the pentose phosphate pathway in frog oocytes is limited by NADP+ availability
In vivo operation of the pentose phosphate pathway in frog oocytes is limited by NADP+ availability.
Ligand-induced conformational transitions in Escherichia coli phosphofructokinase 2: Evidence for an allosteric site for MgATP2-
Ligand-induced conformational transitions in Escherichia coli phosphofructokinase 2: evidence for an allosteric site for MgATP2-.
A mutant phosphofructokinase produces a futile cycle during gluconeogenesis in Escherichia coli
A mutant phosphofructokinase produces a futile cycle during gluconeogenesis in Escherichia coli.
Regulatory role of fructose-2,6-bisP on glucose metabolism in frog oocytes: In vivo inhibition of glycogen synthesis
Regulatory role of fructose-2,6-bisP on glucose metabolism in frog oocytes: in vivo inhibition of glycogen synthesis.
RHCil'latory rolf of frucrosfc-2.6-blsphosphate in THF invivo ilucose metabolism in frog oocytfs
Glycogen synthesis in amphibian oocytes: Evidence for an indirect pathway
Glycogen synthesis in amphibian oocytes: evidence for an indirect pathway.
A new method of assessing rates of the futile cycle during glycolytic and gluconeogenic metabolism.
Fructose-1,6-bisphosphatase in stage VI frog oocytes: evidence for an active enzyme in vivo.
Glycolysis is operative in amphibian oocytes.
The separation and identification of picomole amounts of intermediates of glucose metabolism by high performance liquid chromatography on pellicular resins.
Influence of ligands on the aggregation of the normal and mutant forms of phosphofructokinase 2 of Escherichia coli.
A phosphofructokinase mutant of Escherichia coli altered in its allosteric properties impairs gluconeogenic growth.
Effect of ATP on phosphofructokinase-2 from Escherichia coli. A mutant enzyme altered in the allosteric site for MgATP.
Kinetic mechanism of phosphofructokinase-2 from Escherichia coli. A mutant enzyme with a different mechanism.
Fructose bisphosphatase from Escherichia coli. Purification and characterization.
An alteration in phosphofructokinase 2 of Escherichia coli which impairs gluconeogenic growth and improves growth on sugars.
Comparative studies on glucose phosphorylating isozymes of vertebrates. VII. Mammalian hexokinases
Glucose utilization in vertebrates as a molecular probe for the study of evolution.
Phylogenetic and ontogenetic studies of glucose phosphorylating isozymes of vertebrates.

Abstract (6)

ADP-Dependent Kinases from the Archaeal Order Methanosarcinales Adapt to Salt by a Non-Canonical Evolutionary Conserved Strategy
Direct Observation Of Solvent Penetration During Cold Denaturation Of Pfk-2 By Amide Hydrogen/Deuterium (H/H-2) Exchange Mass Spectrometry
Experimental paleogenetics and paleobiochemistry to study the evolutionary history of substrate specificity in archaeal ADP-dependent sugar kinases family
Role of the conserved N187 residue of E-coli Pfk-2 in catalysis and allosteric regulation
On the catalytic role of conserved active site residues D256 and E190 of Escherichia coli Phosphofructokinase-2, a member of the ribokinase family
Allosteric control of the oligomerization of phosphofructokinase-2 from E-coli.

Proyecto (16)

Unveiling the allosteric regulation of central carbon metabolism in Archaea through kinetic, structural, and evolutive studies of key enzymes.
Spreading the “resolution revolution” across Latin American through cryo-EM studies of a mollusk hemocyanin, with substantial biomedical impact.
Evolutionary trajectories of molecular mechanisms underlying enzyme adaptations to extreme environments.
Unveiling the PET degradation activity of antarctic cutinases via protein engineering
"ANÁLISIS FUNCIONAL Y ESTRUCTURAL DE LA GLUCOQUINASA HUMANA DEPENDIENTE DE ADP."
Temperature and substrate dependent conformational changes of bifunctional ADP-dependent sugar kinases from Archaea.
Tracing the evolution of the ADP-dependent kinase family=> Structural determinants for catalytic properties and thermal adaptations
Degradation of titin at a single molecule level en colaboración con el Dr. Julio Fernández
Large structural changes induced by ligand binding and oligomerization in enzymes from the ribokinase superfamily
Folding of proteins assisted by DNA binding=> role of electrostatic interactions in the domain swapping mechanism of the FOXP and CSP families
Evolution of ribokinase superfamily enzymes=> structure-function relationships that determine substrate specificity, metal assisted mechanism and protein stability
A comprehensive model for the mechanism of allosteric regulation of E. coli phosphofructokinase-2. Integration of kinetic, structural, dynamic and mechanic aspects.
Structural studies in glucokinase of Thermococcus litoralis. Closure domains trigged by ligands and transition state analogs
Filogenia, mecanismo catalítico y especificidad de sustrato en enzimas de la Superfamilia Riboquinasa. Implicaciones para la evolución de la Superfamilia.
Formación e interconversión de estructuras cuaternarias de enzimas=> importancia de las interfaces de Pfk-2 de E. coli para la estabilidad, la actividad catalítica y la regulación alostérica
Análisis estructural y funcional de los sitios de unión de ligandos y control alostérico de la oligomerización de fosfofructoquinasa-2 de E. coli.

Review (1)

The ADP-dependent Sugar Kinase Family: Kinetic and Evolutionary Aspects
69
Victoria Guixé

Titular Profesor

Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

17
Jorge Babul

UNIVERSIDAD DE CHILE

Santiago, Chile

10
CESAR RAMIREZ

Assistant Professor

Institute for Biological and Medical Engineering

Pontificia Universidad Catolica de Chile

Santiago, Chile

10
Víctor Castro

Profesor Asistente

Departamento de Biología

UNIVERSIDAD DE CHILE

Ñuñoa, Chile

10
Mauricio Baez

Assistant Professor

Bioquímica y Biología Molecular

UNIVERSIDAD DE CHILE

Santiago, Chile

8
Ricardo Cabrera

Assitant Pofessor

Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

6
Ricardo Zamora

Researcher Assistant

Universidad de Talca

Talca, Chile

5
Jaime Rivas

Profesor Asociado

Facultad de Ciencias

Universidad Mayor

Santiago, Chile

4
Christian Wilson

Associate professor

Bioquimica y Biologia Molecular

Universidad de Chile

Santiago, Chile

4
Alejandra Herrera

PROFESORA ASISTENTE

QUÍMICA Y FISICA

Universidad Autónoma de Chile

TALCA, Chile

2
Pablo Villalobos

Investigador postdoctoral

Biología

Universidad de Chile

Santiago, Chile

1
Rodrigo Pacheco

Associate Investigator; Head of the Laboratory of Neuroimmunology

Fundación Ciencia para la Vida

Santiago, Chile

1
Gabriel Vallejos

Investigador postdoctoral

Departamento de Biología

Universidad de Chile

Santiago, Chile

1
Mauricio Gonzalez

Profesor Titular

INTA

UNIVERSIDAD DE CHILE - INSTITUTO DE NUTRICION Y TECNOLOGIA DE ALIMENTOS

Santiago, Chile

1
Alvaro Lladser

Professor, Principal Investigator

FUNDACIÓN CIENCIA PARA LA VIDA

Santiago, Chile

1
Jans Alzate

Associate Professor

Departamento de Bioinformática

UNIVERSIDAD DE TALCA

Talca, Chile

1
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

1
Beatriz Diez

PROFESOR ASOCIADO

GENÉTICA MOLECULAR Y MICROBIOLOGIA

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Inmaculada Vaca

Associate Professor

Chemistry Department

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Diego Quiroga

Investigation assistant at Biochemistry Lab (Chem and Pharm Sciences Fac, Dr. Christian AM Wilson)

Universidad de Chile

Santiago, Chile

1
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Jocelyn Neira

Especialista Profesional

Universidad Andrés Bello

Santiago, Chile

1
Sarah Nuñez

Académico Investigador

Facultad de Ciencias de la Salud

Universidad San Sebastián

Santiago, Chile

1
Gabriela Tejon

Investigador Postdoctoral

Biología

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Daniela Sauma

Academic and researcher

Biologia

Universidad de Chile

Santiago, Chile

1
Gonzalo Ureta

Investigador post doctoral

Fundación Ciencia y Vida

Santiago, Chile

1
Gloria Levican

Académica Profesora Titular

Departamento de Biología

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

1
Romina Sepúlveda

Profesor Investigador

Facultad de Ciencias Biológicas

Universidad Andrés Bello

Santiago, Chile

1
Felipe Engelberger

PhD(c)

University of Leipzig

Leipzig, Alemania

1
Fernando Gonzalez

Titular Professor

Facultad de Ciencias de la Vida

Universidad Andres Bello

Santiago, Chile

1
Carlos Gil

Coordinador del area de Biotecnología

Spora SPA

Santiago de Chile, Chile