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Alejandro Andres Hidalgo Cea

Associate Professor

Universidad Andres Bello

Santiago, Chile

Líneas de Investigación


Study essential genes, impact of pseudogenes and how metabolism affects antibiotics resistance in Salmonella. 2 vaccine prototypes with programed death are under testing. We use Classic microbiology, genetics, biochemical and cell biology techniques.

Educación

  •  Ph.D. Biochemistry, State University Of New York, Buffalo. Estados Unidos, 2012
  •  Título de Bioquímico, UNIVERSIDAD DE CHILE. Chile, 2002
  •  Licenciado en Bioquímica, UNIVERSIDAD DE CHILE. Chile, 2001
  •  Postdoc, Cancer therapeutics, ROSWELL PARK CANCER INSTITUTE. Estados Unidos, 2013
  •  Postdoc, Bacterial Genetics, Laboratory of Molecular Pathogensis and anti-microbials, UNAB. Chile, 2016

Experiencia Académica

  •   Postdoc Full Time

    ROSWELL PARK CANCER INSTITUTE

    Buffalo, NY, Estados Unidos

    2012 - 2013

  •   Predoctoral researcher Full Time

    State University of New York - University at Buffalo

    Buffalo, NY, Estados Unidos

    2007 - 2012

  •   Researche assistant Full Time

    Roswell Park Cancer Institute

    Buffalo, NY, Estados Unidos

    2004 - 2006

  •   Associate Professor Full Time

    UNIVERSIDAD ANDRES BELLO

    Medicina

    Santiago, Chile

    2019 - A la fecha

  •   Profesor Asistente Adjunto Full Time

    UNIVERSIDAD ANDRES BELLO

    Medicina

    Santiago, RM, Chile

    2016 - 2019

  •   Profesor Asistente Adjunto Part Time

    UNIVERSIDAD SAN ANDRES

    Ciencias Biológicas

    Santiago, RM, Chile

    2013 - 2016

Experiencia Profesional

  •   Profesor Other

    Universidad Andrés Bello

    Santiago, Chile

    2013 - A la fecha

Formación de Capital Humano


THESES MENTORING

DOCTORAL DEGREE:
Ana R. Millanao, Ph.D. in Pharmaceutical Sciences, Universidad de Chile (Apr 2020)

MASTER DEGREE
Bruno Garces, Master in Biochemistry, Universidad Andrés Bello (Mar 2021)
Nuria C. Gutierrez, Master in Biochemistry, Universidad Andrés Bello (Ene 2019)
Mara B. Hidalgo, Master in Biochemistry, Universidad Andrés Bello (Aug 2017)

UNDERGRADUATED DEGREE
María José Peña, Program of Pharmacy, Universidad Andrés Bello (Jun 2021)
Fabián E. Pérez, Program of Pharmacy, Universidad Andrés Bello (Jany 2017)

THESES CO-MENTORING
Paloma Manosalva, Program of Pharmacy, Universidad Andrés Bello (jan 2020)
Aracely Y. Mora, Master in Biochemistry, Universidad Andrés Bello (oct 2018)
Zvonimir I. Mihovilovic, Program of Pharmacy, Universidad Andrés Bello (May 2017)
Víctor Canales, Master in Biotech. Engineering, Universidad Andrés Bello (sep 2017)

THESES EVALUATION COMMITTEE
José Silva, Program of Pharmacy, Universidad Andrés Bello (jan 2021)
Diego Lorca, undergraduate program in Biochemistry, Universidad Andrés Bello (Jun 2021)
Andrés Silva, Magíster en Biotecnología, Universidad Andrés Bello (may 2020)
Cesar Valenzuela, Master in Biotech. Engineering, Universidad Andrés Bello (may 2020)
Nicolas Opazo, Program of Pharmacy, Universidad Andrés Bello (jan 2020)
Ezequiel Rivas, program of Pharmacy (May 2017), Universidad Andrés Bello
Mauricio Galaz, MS. Biochemistry, Universidad Andrés Bello (Jun 2018)
Cynthia Navarrete, program of Pharmacy (Mayo 2017), Universidad Andrés Bello
Jan Neverman, MS. Biochemistry (September 2016), Universidad Andrés Bello
Silvana Urra, MS. Biochemistry (Mar 2016), Universidad Andrés Bello
Alan Briones, MS. Biochemistry (Mar 2016), Universidad Andrés Bello
Ana inostroza, MS. Biochemistry (Jan 2016), Universidad Andrés Bello
Catalina Díaz, MS. Biochemistry (Sep 2015), Universidad Andrés Bello
Carolina Cabezas, MS. Biochemistry (Apr 2015), Universidad Andrés Bello
Mackarena Contreras, MS. Biochemistry (Jun 2014), Universidad Andrés Bello

TECHING EXPERIENCE

General Microbiology I, BIOL250 for the Program in Pharmacy (03/2016-present)
School of Medicine, Universidad Andrés Bello

General Biochemistry I, BIOL260 for the Program in Pharmacy (03/2019-present)
School of Medicine, Universidad Andrés Bello

Laboratory of Microbiology I, BIOL251 for the Program in Pharmacy (during 2016)
School of Medicine, Universidad Andrés Bello

Invited Professor, Bio396 mechanisms of gene expression in prokaryotic organisms (07/2015-present) Class covering topics of transcriptional termination, School of Biological Sciences, Universidad Andrés Bello

Invited Professor, Molecular Microbiology of Intracellular pathogenic bacteria (2014-present)
Class: Interaction of Salmonella with the host cell
Course for graduate students, Program of Microbiology, School of Medicine, University of Chile

Laboratory of Microbiology II, Bio351 (2013-present)
Theoretical/practical course on topics of bacterial genetics, for students of Biochemistry in their 4th Year School of Biological Sciences, University Andrés Bello


Difusión y Transferencia


MEETING ATTENDANCE ( * indicates oral presentation, first or last author only, from over 40 presentations, last 5 years)

Mora A, Jerez S, Millanao A, Villagra A, Hidalgo AA. “Engineered Salmonella strains, with tetracycline-dependent growth features, to be used as vaccines and delivery vectors.” XLI Congreso SOMICH, november 2019, Puerto Varas, Chile
Hidalgo AA, Fuentes JA, Arias AJ, Mora GC, Villagra NA. “Nueva Aproximación para disminuir la resistencia antimicrobiana, mediada por bombas de expulsión, en patógenos de importancia médica.” XLI Congreso SOMICH, november 2019, Puerto Varas, Chile
Mora AY, Villagra NA, Mora GC, Hidalgo AA. La pseudogenización del gen tsr, un sensor del aceptor alternativo de electrones nitrato, modula la patogenicidad de Salmonella enterica serovar Typhi. XXIV Congreso ALAM, Noviembre 2018, Santiago, Chile
Gutiérrez NC, Hidalgo MB, Mora AY, Hidalgo AA. YqgF regulates the expression of genes involved in the pathogenicity of S. Typhimurium. XXIV Congreso ALAM, Noviembre 2018, Santiago, Chile
Mora AY, Villagra NA, Mora GC, Hidalgo AA. “The loss of genes involved in sensing alternative electron acceptors modulates the pathogenicity of Salmonella enterica serovar Typhi. ”XXXIX Congreso SOMICH, Noviembre 2017, La Serena, Chile
Gutierrez NC, Hidalgo MB, Mora AY, Hidalgo AA. “Transcriptional changes of genes regulated by yqgF gene product in Salmonella Typhimurium.” XXXIX Congreso SOMICH, Noviembre 2017, La Serena, Chile
Hidalgo MB, Villagra NA y Hidalgo AA. “Regulación de la expresión del gen yqgF usando un sistema inducible en Salmonella.” XXXVIII Congreso SOMICH, December 2016 , Valdivia, Chile
Pérez FE, Rodríguez M, Villagra NA y Hidalgo AA. El lipopolisacárido como determinante de la resistencia a extractos vegetales con actividad antimicrobiana. XXXVIII Congreso SOMICH, December 2016, Valdivia, Chile
*Hidalgo AA. “A conditionally lethal mutant of Salmonella Typhimurium induces strong humoral-immune response in mice.” XXXVII Congreso SOMICH, Decembre 2015, La Serena , Chile


Premios y Distinciones

  •   CONICYT Fellowship for doctoral studies in foreign countries

    CONICYT gestión propia

    Estados Unidos, 2008

    Included stipend and living costs

  •   Predoctoral traineeships-DOD, Department of Defense, United States

    Department of Defence - USA

    Estados Unidos, 2007

    Funding for stipend and laboratory supplies

  •   Achievement Award, Recruitment and Retention Funding, SUNY at Buffalo

    STATE UNIVERSITY OF NEY YORK AT BUFFALO

    Chile, 2017

    Price for academic achievements during first year of doctoral studies

  •   IGPBS Fellowship, SUNY at Buffalo

    STATE UNIVERSITY OF NEY YORK AT BUFFALO

    Chile, 2006

    Fellowship to cover stipend during the first year at the “Interdisciplinary Graduate Program in Biomedical Sciences”


 

Article (27)

Inactivation of Glutamine Synthetase-Coding Gene glnA Increases Susceptibility to Quinolones Through Increasing Outer Membrane Protein F in Salmonella enterica Serovar Typhi
Cysteine auxotrophy drives reduced susceptibility to quinolones and paraquat by inducing the expression of efflux-pump systems and detoxifying enzymes in S. Typhimurium
The Transcription Factor ArcA Modulates Salmonella's Metabolism in Response to Neutrophil Hypochlorous Acid-Mediated Stress
The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium
The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite
Xylose Improves Antibiotic Activity of Chloramphenicol and Tetracycline against K. pneumoniae and A. baumannii in a Murine Model of Skin Infection
A conditionally lethal mutant of Salmonella Typhimurium induces a protective response in mice
Lose to win: marT pseudogenization in Salmonella enterica serovar Typhi contributed to the surV-dependent survival to H2O2, and inside human macrophage-like cells
Lose to win=> marT pseudogenization in Salmonella enterica serovar Typhi contributed to the surV-dependent survival to HO, and inside human macrophage-like cells.
SPI-9 of Salmonella enterica serovar Typhi is constituted by an operon positively regulated by RpoS and contributes to adherence to epithelial cells in culture
SPI-9 of Salmonella enterica serovar Typhi is constituted by an operon positively regulated by RpoS and contributes to adherence to epithelial cells in culture.
Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi
RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression
RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression.
Salmonella Typhi shdA: Pseudogene or allelic variant?
Inecalcitol, an analog of 1,25D(3), displays enhanced antitumor activity through the induction of apoptosis in a squamous cell carcinoma model system
Inecalcitol, an analog of 1,25D3, displays enhanced antitumor activity through the induction of apoptosis in a squamous cell carcinoma model system
The carbon source influences the efflux pump-mediated antimicrobial resistance in clinically important Gram-negative bacteria
Biochemical characterization of nuclear receptors for vitamin D-3 and glucocorticoids in prostate stroma cell microenvironment
Dexamethasone Enhances 1 alpha,25-Dihydroxyvitamin D-3 Effects by Increasing Vitamin D Receptor Transcription
Dexamethasone enhances 1,25-dihydroxyvitamin D3 effects by increasing vitamin D receptor transcription
Glucocorticoid regulation of the vitamin D receptor
SmvA, and not AcrB, is the major efflux pump for acriflavine and related compounds in Salmonella enterica serovar Typhimurium
Altered VDR-mediated transcriptional activity in prostate cancer stroma
Hypoxia increases androgen receptor activity in prostate cancer cells
Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi
Global regulation of the Salmonella enterica serovar Typhimurium major porin, OmpD

Proyecto (6)

IDENTIFICATION OF GENES REGULATED BY YQGF, A NOVEL ANTI-RHO FACTOR THAT DIFFERENTIALLY CONTROLS THE FITNESS OF SALMONELLA IN VITRO AND IN THE INTRACELLULAR MICROENVIRONMENT
LOSS OF KEY FUNCTIONS DRIVES SALMONELLA TYPHI ADAPTIVE PATHOGENESIS TO HUMANS
Diseño de una formulación para uso farmacéutico efectiva contra bacterias multirresistentes de importancia médica
MUTANTES CONDICIONALES DE SALMONELLA PARA SER USADAS COMO VECTORES
Molecular Mechanisms of Differential Effects of Calcitriol on Endothelial Cells Isolated from Prostate Tumor and Normal Microenvironment
CARACTERIZACION GENETICA DE LOS MECANISMOS MOLECULARES DE LA PATOGENICIDAD DE SALMONELLA TYPHI=> INVACION A CELULAS EPITELIALES Y ESPECIFICIDAD DE HOSPEDERO.
24
Alejandro Hidalgo

Associate Professor

Escuela de Química y Farmacia

Universidad Andres Bello

Santiago, Chile

9
Guido Mora

Académico/Investigador

Facultad Ciencias de la Salud

Universidad SEK

Santiago, Chile

7
Claudia Saavedra

Full Professor

Departmento de Ciencias de la Vida

UNIVERSIDAD ANDRÉS BELLO

Santiago, Chile

7
Juan Fuentes

Académico

Ciencias Biológicas

Universidad Andrés Bello

Santiago, Chile

4
Nicolás Villagra

Profesor Asistente

Tecnología Médica

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

3
Alan Briones

Bioquímico Clínico

Hospital San Juan de Dios

Santiago, Chile

3
Coral Pardo

Investigador postdoctoral

Universidad Andrés Bello

Santiago, Chile

3
Juan Castro

Post-Doctorante

Universidad Católica del Norte

Antofagasta, Chile

2
Viviana Montecinos

Assistant Professor

Hematology Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

2
Eduardo Castro

Professor

Departamento de Microbiología

Universidad de Talca

Talca, Chile

2
Claudio Meneses

Profesor Asociado

Facultad de Agronomía e Ingeniería Forestal/ Facultad de Ciencias Biológicas

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Sergio Onate

Associate Professor

Medical Specialties

UNIVERSIDAD DE CONCEPCION, DEPARTAMENTO FISIOPATOLOGIA

concepcion, Chile

1
Patricia García

Jefe de Laboratorio de Microbiologia

Escuela de Medicina

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE, LABORATORIO DE MICROBIOLOGÍA

Santiago, Chile

1
Juan Castro

Researcher

Universidad de Chile

Santiago, Chile

1
IVAN CALDERON

Associate Professor / Academic Coordinator / Principal Investigator

Biological Sciences

UNIVERSIDAD ANDRÉS BELLO

SANTIAGO, Chile

1
Maria Otero

Profesor Asociado

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Fernando Gil

Associate Professor

Biological Sciences

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Maria Opazo

Profesor honorarios

Ciencias Biologicas

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Daniel Paredes

Associate Professor

Department of Biological Sciences

Universidad Andrés Bello

Santiago, Chile

1
Ignacio Poblete

Professor

Universidad Andres Bello

santiago, Chile

1
Gabriel Krüger

Investigador Doctoral

Universidad Andrés Bello

Santiago Centro, Chile

1
Rodrigo Pacheco

Associate Investigator; Head of the Laboratory of Neuroimmunology

Fundación Ciencia para la Vida

Santiago, Chile