Man

Maria Alejandra Loyola Pedevila

Principal Investigator

Fundación Ciencia & Vida

Santiago, Chile

Líneas de Investigación


1. Analysis of the establishment of newly synthesized histones H3 and H4 modifications. 2. Investigation of the epigenetic regulation of the hepatitis B virus replication. 3. Studies on the epigenetic mechanisms of T lymphocyte differentiation.

Educación

  •  Biochemist, UNIVERSIDAD DE CHILE. Chile, 1996
  •  Biomedical Sciences, RUTGERS UNIVERSITY. Estados Unidos, 2003

Experiencia Académica

  •   Post-doctoral fellow Full Time

    INSTITUT CURIE DE PARIS

    Paris, Francia

    2003 - 2005

  •   post-doctoral fellow Full Time

    UNIV TEXAS M.D. ANDERSON CANCER CENTER

    Houston, Estados Unidos

    2006 - 2008

  •   Principal Investigator Full Time

    Fundación Ciencia & Vida

    Santiago, Chile

    2008 - A la fecha

Experiencia Profesional

  •   Post-doctoral fellow Full Time

    Institut Curie

    Paris, Francia

    2003 - 2005

  •   Post-doctoral fellow Full Time

    MD Anderson Cancer Center

    Houston, Estados Unidos

    2006 - 2008

  •   Principal Investigator Full Time

    Fundación Ciencia & Vida

    Santiago, Chile

    2008 - A la fecha

Formación de Capital Humano


UNDERGRADUATE AND GRADUATE TRAINING
(1) Francisca Álvarez Astudillo. “Análisis de la función de las modificaciones post-traduccionales en las histonas citos?licas H3/H4”. Tesis para obtener el título profesional de Bioquímico. Pontificia Universidad Católica de Chile. Mayo, 2009-Abril, 2011.
(2) Francisca Muñoz Garrido. “Función de las modificaciones post-traduccionales de las histonas en el transporte al núcleo”. Tesis para obtener el título profesional de Bioquímico. Pontificia Universidad Católica de Chile. Septiembre, 2009-Mayo, 2011.
(3) Valentina Ugalde Tagle. “Rol de la enzima que metila H3K9 SetDB1”. Tesis para obtener el título profesional de Bioquímico y Magíster. Universidad Nacional Andrés Bello. Agosto, 2009-Noviembre, 2011.
(4) Macarena Fritz Kelly, “Mecanismos epigenéticos implicados en la reprogramación de linfocitos Treg a linfocitos Th17”, Doctorado en Biotecnología, Universidad Andrés Bello. 2009-Diciembre, 2013.
(5) Francisco Saavedra Cantillana, “Caracterización de los complejos de translocación nuclear de las histonas H3 y H4”. Tesis para obtener el título profesional de Ingeniero en Biotecnología. Universidad de Chile. Agosto, 2012- Noviembre, 2014.
(6) Cristian Doñas Cuadra, “Modulación de la diferenciación de linfocitos T CD4+ mediante el uso de fármacos epigenéticos”. Tesis para obtener el título profesional de Bioquímico y Magíster. Universidad Andrés Bello. Agosto, 2011- Noviembre, 2014.
(7) Germán Púas Villegas, “Análisis de la dinámica de los complejos citosólicos de las histonas H3 y H4 al inhibir la degradación proteica”. Tesis para obtener el título profesional de Ingeniero en Biotecnología. Universidad Iberoamericana de Ciencia y Tecnología. Marzo, 2012-Noviembre, 2014.
(8) Carlos Rivera Alvarez, “Caracterización del complejo citosólico II de la histona H3 en células HeLa”. Tesis para obtener el título profesional de Bioquímico y Magíster. Universidad de Chile. Marzo, 2013-Enero 2015.
(9) Yvo Flores León, “Análisis del perfil de metilación de DNA en el genotipo F del virus de la hepatitis B”. Tesis para obtener el título profesional de Bioquímico y Magíster. Universidad Andrés Bello. Marzo, 2013-Abril, 2015.
(10) Lorena Rubio Quiroz, “Efecto del complejo metiltransferasa de la lisina 4 de la histona H3 COMPASS sobre la replicación y actividad transcripcional del virus de hepatitis B”. Magíster en Biotecnología. Universidad Andrés Bello. Marzo, 2013-Marzo 2016.
(11) Jocelyn Neira, “Efecto de GSK-J4, un inhibidor de las desmetilasas de la histona H3, en enfermedades inflamatorias intestinales”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2011-Enero 2017.
(12) Daniel Garrido Norambuena, “Caracterización de las isoformas de la proteína X (HBx) del virus de la hepatitis B (HBV)”. Tesis para obtener el título profesional de Bioquímico y Magíster. Universidad Andrés Bello. Marzo, 2015-Marzo, 2017.
(13) Sergio Hernández Tapia, “Estudio de las isoformas de la proteína x del virus de la hepatitis B y su rol en la replicación viral”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2013-Abril, 2017.
(14) Claudia Espinoza, “Estudio de la relación entre el Complejo II de las histonas citosólicas y la degradación de proteínas mal plegadas”. Tesis para obtener el título profesional de Bioquímico. Universidad de Chile. Abril, 2015-Mayo, 2017.
(15) Sebastián Marty Lombardi, “Asociación de SetDB1 a los ribosomas durante el ciclo celular y su efecto sobre H3K9me1 citosólico”. Tesis para obtener el título profesional de Ingeniería en Biotecnología Molecular. Universidad de Chile. Marzo, 2016-Junio, 2017.
(16) Cristian Doñas Cuadra, “Inhibición farmacológica del desarrollo de enfermedades autoinmunes y del rechazo al trasplante de órganos”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2013-Junio, 2017.
(17) Francisca Muñoz Garrido, “Rol de la cromatina viral en la replicación del virus de la hepatitis B”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2011-Mayo, 2018.
(18) Francisca Alvarez Astudillo, “Estudio del establecimiento de los patrones de metilación de la histona H3, su ensamblaje en el minicromosoma del virus de la hepatitis B y su función en la replicación viral”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2014-Octubre, 2018.
(19) Francisco Saavedra Castillana, “La desmetilasa de histonas JMJD1B participa en el procesamiento temprano de la histona H3 citosólica”, Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2014-Enero, 2019.
(20) Pilar Castillo, “Análisis del efecto de complejos que remodelan cromatina en la replicación y transcripción del virus de la hepatitis B”. Tesis para obtener el título profesional de Bioquímico. Universidad de Concepción. Abril, 2015-Mayo, 2019.
(21) Juan Hormazábal, “Análisis de la degradación de histonas citosólicas a través de la autofagia mediada por chaperonas”. Doctorado en Biotecnología, Universidad Andrés Bello. Marzo, 2015-Abril, 2020.
(22) Tatiana Cruces Ayala, “Caracterización del interactoma de la proteína JMJD1B”. Tesis para obtener el título profesional de Ingeniería en Biotecnología Molecular. Universidad de Chile. Septiembre, 2018-Junio, 2020.

POSTDOCTORAL TRAINING
(1) César Díaz Célis: “Methylation of histone H3K9 occurs during translation”. December 2011-December 2012.
(2) Paola Merino: “PP32 and SET proteins regulate the establishment of newly synthesized histone H4 acetylation”. March 2015-December 2015.
(3) Rodrigo Flores: “Study of the function of histone-like proteins in the regulation of gene expression of mobile genetic element encoded-genes in acidophilic bioleaching bacteria”. July-2018.
(4) Pía Vidal: Epigenetic control of transcriptional networks promoting the pathogenic behavior of CD4+ T-cells in inflammatory bowel disease”. July-2018.
(5) Francisco Saavedra: “JMJD1B, un nuevo regulador en el procesamiento de histonas H3 and H4 que asegura la estabilidad genómica”. Marzo, 2019-presente.
(6) Simon Beard: “Study of the function of histone-like proteins in the regulation of gene expression of mobile genetic element encoded-genes in acidophilic bioleaching bacteria”. Agosto 2019-present.
(7) Jose Manuel Jimenez: “Epigenetic regulation of transcriptional networks promoting the pathogenic behavior of CD4+ T-cells in inflammatory bowel disease”. January 2020-present.


Difusión y Transferencia


1) Scientific editor of the third serie of the cartoon Ursi y Magnogeek. These are short animations, called “Píldoras Científicas”, consist in 5 minutes videos, directed to 7-12 year old kids to explain basic concepts of science .The animations are made with the Eureka team, headed by Pablo Rosemblatt, at Fundación Ciencia & Vida (http://www.canal-eureka.cl/). The series covered 5 chapters that focus in genomic information, cancer, and viruses. This series is freely distributed through our web-page as well as to public schools through Red Novasur. The serie was launch on June 18th, 2015, at the Museo Interactivo Mirador. About 50 kids participated in this event.
2) Every year undergraduate and graduate students from the lab participate in Explora activities giving lectures to high school students. As an example, “Transgénicos: ¿son realmente malos?". Colegio Raimapu Tierra Florida, La Florida (13 de Octubre 2014 by Francisca Alvarez). ¿Qué es la vida?. Colegio Germina, Puerto Varas (4 de Octubre 2012). “Experiencia científica de ex-alumnos”. Colegio Santa María, Quilicura (18 Noviembre 2013 by Francisco Saavedra). “Taller de extracción de ADN” Colegio Germania (2013).
3) Every year our laboratory receives students from different schools, which came to our institution to learn about our research.
4) Cristian Doñas, Jocelyn Neira, PhD students of the lab participated in Bus Conciencia, a bus bearing a laboratory inside which aims to visit different schools to show and teach school students how scientists perform science (2012-2015).
5) Participation in Taller de Líderes de Opinión (Alejandra Loyola, 2012-2015).
6) Desafío levantemos Chile/Primera Infancia: diseño de un programa científico anual para desarrollar en 90 jardines infantiles de la V, VIII y IX región, correspondiente a 1843 pre-escolares en situación de extrema pobreza (Alejandra Loyola, Francisca Muñoz, Francisca Alvarez , 2012).
7) Desafío Levantemos Chile/Primera Infancia: capacitación a profesores de la comuna de Viña del Mar, Llay-Llay, Concepción y Temuco, acerca del programa científico anual desarrollado (Alejandra Loyola, Francisca Muñoz, Francisca Alvarez, 2013-2014).
8) Apadrinamiento del taller de ciencias del Colegio Guardiamarina Guillermo Zañartu, 2018, Santiago, Chile.
9) Entrevista “Exploradores del átomo al cosmos”, Canal 24 horas.


Premios y Distinciones

  •   Editorial Borad of Biochemistry and Cell Biology

    NRC Research Press

    Canada, 2014

    Editorial Board of the journal Biochemistry and Cell Biology


 

Article (28)

Parental nucleosome segregation and the inheritance of cellular identity
Phosphorylation of phylogenetically conserved amino acid residues confines HBx within different cell compartments of human hepatocarcinoma cells
The demethylase inhibitor GSK-J4 limits inflammatory colitis by promoting de novo synthesis of retinoic acid in dendritic cells
The Histone Variant H3.3 Regulates the Transcription of the Hepatitis B Virus
JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability
Recombinant HCV NS3 and NS5B enzymes exhibit multiple posttranslational modifications for potential regulation
Characterization of Posttranslational Modifications on Histone Variants.
PP32 and SET/TAF-I beta proteins regulate the acetylation of newly synthesized histone H4
Replication of a chronic hepatitis B virus genotype F1b construct
The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state
The histone demethylase inhibitor GSK-J4 limits inflammation through the induction of a tolerogenic phenotype on DCs
Methylation of histone H3 lysine 9 occurs during translation
Wnt signaling induces transcription, spatial proximity, and translocation of fusion gene partners in human hematopoietic cells
Histone lysine methylation and chromatin replication
Trichostatin A Promotes the Generation and Suppressive Functions of Regulatory T Cells
Sequential Establishment of Marks on Soluble Histones H3 and H4
The HP1 alpha-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin
Marking histone H3 variants: how, when and why?
PTMs on H3 Variants before Chromatin Assembly Potentiate Their Final Epigenetic State
Bromodomains in living cells participate in deciphering the histone code.
Histone chaperones, a supporting role in the limelight.
Techniques used to study transcription on chromatin templates.
Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis.
Functional analysis of the subunits of the chromatin assembly factor RSF.
Histone deposition and chromatin assembly by RSF.
The constantly changing face of chromatin.
Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription.
Purification and characterization of a human factor that assembles and remodels chromatin.

Abstract (1)

Wnt/beta-catenin signaling enhances RUNX1 expression in leukemia cells and hematopoietic progenitors.

BookSection (1)

Histone variants: Structure, function and implication in diseases

ConferencePoster (26)

Cold Spring Harbor Meeting. Epigenetics & Chromatin
42nd Congress of the Spanish Society of Biochemistry and Molecular Biology
EMBO Workshop on Histone chaperones: Structure, function and role in development and disease
H3K9 methylation on ribosomes regulates the maturation of newly synthesized histone H3
Newly synthesized histone H3 is degraded by chaperone mediated autophagy
Processing and maturation of newly synthesized histones H3 and H4
Association of SetDB1 to ribosomes during the cell cycle and its impacts on the H3K9me1 mark
Establishment of post-translational modifications on newly synthesized histones H3 and H4
Establishment of PTMs on newly synthesized histones H3 and H4
GSK-J4 a selective inhibitor of the H3K27 demethylase JMJD3/UTX limits acute colitis by the novo synthesis of retinoic acid in dendritic cells
The hepatitis B viral protein HBx is required to establish an active hepatitis B viral chromatin state
The small isoform of the HBx protein is sufficient to establish an active hepatitis B viral chromatin state
Characterization of the subcellular localization of the protein X (HBx) isoforms of hepatitis B virus (HBV)
GSK-J4 a potent and selective inhibitor of the H3K27 demethylase JMJD3/UTX limits inflammation by favoring a tolerogenic potential in dendritic cells
JMJD1B participates in the processing of cytosolic histone H3
Misfolded newly synthesized histone H3 is degraded by the ubiquitin-proteasome system
A selective H3K27 demethylase inhibitor ameliorates experimental acute colitis
PP32 and SET regulates the establishment of H4 acetylation on newly synthesized histones
SETDB1 associates to ribosomes and methylates ribosome-bound lysine 9 of histone H3
The histone demethylase LSD1 and the viral protein HBx cooperate to establish an active hepatitis B viral chromatin state
The selective jumonji H3K27 demethylase inhibitor GSK-J4 limits inflammation through dendritic cell modulation
Characterization of the ADP-Ribosylation mark on cytosolic histones H3 and H4
Cloning and replication of a chronic Hepatitis B Virus genotype F isolate
Effect of histone demethylase- and methyltransferase- inhibitors over replication and transcriptional activity of the Hepatitis B Virus
Spatial proximity of translocation partners RUNX1 and RUNX1T1 in hematopoietic stem cells: Role of Wnt/β-catenin signaling
The establishment of the cytosolic H3K9me1 modification

ConferenceProceedingWhole (3)

The processing and maturation of newly synthesized histones
JMJD1B regulates the processing of cytosolic histone H3
The establishment of the post-translational modification H3K9me1 on cytosolic histones H3

Patent (1)

The use of GSK-J4 in prevention and/or treatment of transplant rejection

Proyecto (11)

Fundacion Ciencia y Vida
UNCOVERING THE MECHANISMS OF PROCESSING OF NEWLY SYNTHESIZED HISTONES H3 AND H4
ESTABLISHMENT OF THE MODIFICATIONS ON SOLUBLE HISTONES H3 AND H4
Molecular Mechanisms of Cancer=> examining chromatin function in HBV replicacion and leukemia
Molecular mechanisms of cancer=> examining chromatin function in HBV replication and leukemia.
Epigenetic regulation of the Hepatitis B Virus replication
IN VITRO ASSEMBLY OF A FUNCTIONAL HCV REPLICATION COMPLEX, AND THE ROLE OF THE PHOSPHORYLATION OF THE VIRAL RNA POLYMERASE DURING THE INFECTIVE CYCLE.
CHARACTERIZATION OF THE NON-NUCLEOSOMAL HISTONE H3.1 AND H3.3 POST-TRANSLATIONAL MODIFICATION PATTERNS
Functional Connections between the MLL Methyltransferase and Aurora Kinase B
Epigenetic maps in Xenopus laevis development and Nuclear Reprogramming
Molecular Mechanisms of Nuclear Reprogramming
52
Maria Loyola

Principal Investigator

Fundación Ciencia & Vida

Santiago, Chile

3
Giancarlo De Ferrari

Full Professor

Biological Sciences

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

2
Alvaro Elorza

ASSOCIATE PROFESSOR

INSTITUTE OF BIOMEDICAL SCIENCES

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

2
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

1
MANUEL VARAS

Research Professor

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

1
Rodrigo Pacheco

Associate Investigator; Head of the Laboratory of Neuroimmunology

Fundación Ciencia para la Vida

Santiago, Chile

1
Ivan Alfaro

Associate Professor, Director of Innovation and Technology Development

Faculty of Medicine

Universidad del Desarrollo

Santiago, Chile

1
Pablo León

Asistente de Investigación

Ciencias Biomedicas

Universidad de Chile

Santiago, Chile

1
Carolina Prado

Investigador Joven

Fundacion Ciencia y Vida

Santiago, Chile

1
Gabriela Tejon

Investigador Postdoctoral

Biología

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Raquel Quatrini

Research Associate

Fundación Ciencia & Vida

Santiago, Chile