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Claudia Renate Stange Klein

Titular Professor

UNIVERSIDAD DE CHILE

Santiago, Chile

Líneas de Investigación


Carotenoid biosynthesis regulation in Daucus carota L. (carrot). Improving nutritional value in apples by increasing carotenoid levels. Role of carotenoid in Abiotic stress tolerance in plants

Educación

  •  Plant Molecular Biology, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2004
  •  Biochemist, UNIVERSIDAD DE CHILE. Chile, 1995

Experiencia Académica

  •   Associate Professor Full Time

    UNIVERSIDAD DE CHILE

    Faculty of Science

    Santiago, Chile

    2014 - 2020

  •   Assitant Professor Full Time

    UNIVERSIDAD DE CHILE

    Faculty of Science

    Santiago, Chile

    2007 - 2014

  •   Postdoctoral Full Time

    UNIVERSIDAD DE CHILE

    Faculty of Science

    Santiago, Chile

    2005 - 2007

  •   Docente Full Time

    Universidad del Desarrollo

    of Medicine

    Santiago, Chile

    2004 - 2005

  •   Profesor Titular Full Time

    UNIVERSIDAD DE CHILE

    Facultad de Ciencias

    Santiago, Chile

    2020 - A la fecha

Experiencia Profesional

  •   Associate Professor Full Time

    Universidad de Chile

    Santiago, Chile

    2014 - 2020

  •   post doc Full Time

    Universidad de Chile

    Santiago, Chile

    2005 - 2007

  •   Assistant Professor Full Time

    Universidad de Chile

    Santiago, Chile

    2007 - 2014

  •   Titular Professor Full Time

    Universidad de Chile

    Santiago, Chile

    2020 - A la fecha

Formación de Capital Humano


Post doctoral Supervision

1. Dr. Paulina Fuentes. Fondecyt 1180747 GROWING IN THE DARK: THE ROLE OF LIGHT-INDUCIBLE GENES IN CAROTENOID BIOSYSNTHESYS REGULATION IN CARROT STORAGE ROOTS. 2019-
2. Dr. Carlos Flores. 17CONTEC-8361183611 "Generación de nuevos genotipos de manzano con propiedades funcionales especialmente adaptadas para el consumo fresco y procesado” 2018- until today
3. Dra. Francisca Godoy. Corfo prestación de servicios y 17CONTEC-8361183611 "Generación de nuevos genotipos de manzano con propiedades funcionales especialmente adaptadas para el consumo fresco y procesado” 2018- until today
4. Dra. (c) Anita Arenas. Proyecto Fondef D10I1022 " Uso de Ingeniería Metabólica para el desarrollo de plántulas de manzano que poseen genes para la síntesis de vitamina a y antioxidantes en los frutos”. Enero 2014 -enero 2015.
5. Dr. Daniel Laporte. Proyecto Fondef D10I1022 " Uso de Ingeniería Metabólica para el desarrollo de plántulas de manzano que poseen genes para la síntesis de vitamina a y antioxidantes en los frutos”. Noviembre 2011-Noviembre 2013 .

Doctoral Thesis supervision
1. Luis Felipe Quiroz. Programa Doctorado en Biotecnología Molecular, Facultad de Ciencias, Universidad de Chile. starting thesis: 2015, finishing may 2019.
2. Daniela Arias. Programa Doctorado en Biotecnología Molecular. Facultad de Ciencias, Universidad de Chile. starting thesis: 2015, finishing may 2019
3. Rocío Quian. Programa Doctorado en Biotecnología Molecular. Facultad de Ciencias, Universidad de Chile. starting thesis: 2019

Master thesis supervision 2012 until to day
1. Jonathan Canales, 2017-2019. Caracterización funcional del gen DcPIF3 en el modelo Arabidopsis thaliana. Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile. In progress
2.Angélica Ortega, 2017-2019. Caracterización funcional del gen DcPAR1 en el modelo Arabidopsis thaliana.Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile. in progress.
3 Daniela Arias, 2016. Expresión de genes de síntesis de carotenoides en Solanum lycopersicum. Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.Entregado.
4. Hita Barraza, Septiembre 2015 Evaluación de la tolerancia a estrés abiótico en plantas de tabaco que expresan genes de síntesis de carotenoides. Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.
5. Kevin Simpson, Septiembre 2015. “Identificación de factores de transcripción capaces de unirse y regular al promotor del gen PSY2 de D. carota.” Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.
6. Ariel Cerda, Julio 2015. Caracterización fisiológica y molecular de plantas de Nicotiana tabacum que expresan el gen Lcyb1 de Daucus carota.” Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.
7. Carolina Rosas, Junio 2015, "Estudio de la funcionalidad del gen Lcyb2 de Daucus carota mediante el uso de modelos bacteriano y vegetal" Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.
8. Orlando Acevedo, Marzo 2014, Evaluación de la Funcionalidad del gen fitoeno sintasa 2 (Psy2) de Daucus carota y su participación en la tolerancia al estrés salino en el modelo vegetal Nicotiana tabacum" Magíster en Ciencias Biológicas, Facultad de Ciencias, Universidad de Chile.
9. Juan Camilo Moreno, Marzo 2012, "Caracterización del gen licopeno ?-ciclasa (Lcyb1) de Daucus carota (zanahoria) mediante sobre expresión en modelos vegetales (N. tabacum y D. carota)” Magister en Ciencias Biológicas, Facultad de Ciencias Universidad de Chile.

Undergraduate thesis supervised from 2012 until today:
1. Gerardo Correa 2019 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile. Writing in progress.
2. Felipe Durán 2019. Título de Bioquímico, Universidad de Chile. Writing in progress.
3. María Jesús Gutierrez 2019 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile. Writing in progress.
4. Christian Gonzalez 2018. Título Bioquímico, Universidad de Santiago
5. Agustín Aguilera, 2018. Título de Bioquímico, Universidad de Chile.
6. Lisette Colomer, 2017. Título de Bioquímico, Universidad de Chile.
7. Leonardo Molineros, Junio 2017 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
8. Arturo Lamilla, 2016 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
9. Luis Felipe Quiroz, 2014. Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
10. Daniel Acosta, 2014. Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
11 Magaly Alvarez, 2013. Título de Biotecnóloga de la Universidad de Sta María, Arequipa, Perú.
12 Kevin Simpson, 2013 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
13. Ariel Cerda, 2013 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
14. Gonzalo Diaz, 2013 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.
15. Clio Peirano, 2013 Título de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, U. de Chile.


Difusión y Transferencia


Dissemination and Transfer since 2012 until today.
Participation and interview in Radio Universidad de Chile in Program "I want to be Scientific" directed by Hortensia Morales. March 2012 to 2018.
Visits to the Center of Plant Molecular Biology of teachers and students of different schools once every two months (2012 to 2018) where the students see the work that is done.
Talk about transgenic plants and in vitro culture of plants. Leonardo da Vinci College, Talagante. October 2012
Interview on plants and transgenic foods, Channel Comunal TV, la Pintana.2012
INNOVA Project 07CN13PBD19 and FONDEF D10I1022 on metabolic engineering in apples to increase vitamins and sweetness: Interview Diario Financiero (July 2012), Interview TVN (July 2012), Entrevista Portalfrutícola (August 2012)
Participation in activity 170 "Schools - Academic and Academics" carried out by the University of Chile through its Vice-Rectory of Extension. The talk "Vitamin consumption in the diet" was presented to students of the 5th basic year of the Sol Naciente School, Conchalí. November 2013.
Interview on Project FONDEF D10I1022 and INNOVA 07CN13PBD19 for the German magazine CAMCHAL.DE. August 2013
Interview on Plants and transgenic foods. UCVTV TV channel. Report in section of the news.enero 2013
Talk "The fascinating world of Vegetal genetics" to Liceo 7 (October 2014) and in the Cultural Center of San Antonio to 250 students of 3rd and 4th Middle Schools of San Antonio. Organized by I. Municipality of San Antonio and Faculty of Sciences, U. Chile (March 2014).
International Workshop "Patenting Plant Biology added value to knowledge", November 11-14, 2014. Targeting all citizens
"Scientist Comment" on RedCiencia http://youtu.be/OEX1fUY8Mkw (January 2015)
Interview Magazine "Revista El sabado" from the Mercurio daily on prospecting of biotechnology careers in Chile. Nov 2015
A talk to students of the school Manuel de Salas on "Genetically modified food", April 2016 and june 2018.
Interview on Biotechnology, in El Mercurio, http://www.edicionesespeciales.elmercurio.com/destacadas/detalle/index.asp?idnoticia=201611242381868&idcuerpo= November 2016.
Entrevista sobre alimentos y plantas transgénicas, pagina U de Chile. http://www.uchile.cl/noticias/135227/investigadora-descarta-peligros-de-alimentos-transgenicos (20 de julio 2017)
-Entrevista sobre ingeniería genética y mejoramiento genético de plantas por Chilebio https://drive.google.com/file/d/0BzJnZExRzaSCa3ltOE1yQWoxZU0/view?invite=COK-z7MG&ts=59a5c3ca , agosto 2017
-Entrevista sobre "composición de los alimentos procesados y si tienen compuestos de origen transgénico", Patricio Saavedra Guerin, magister en Prensa Escrita de "El Mercurio" con la PUC (Septiembre , 2017)
-Entrevista sobre transgénicos con periodista Francisca Collao, La Fábrica Imaginaria, cuenta de Chile Global Biotec. (Octubre 2017).  http://www.chileglobalbiotec.cl/2017/11/07/dra-claudia-stange-los-movimientos-anti-transgenia-se-basan-en-el-desconocimiento-y-la-naturaleza-humana-de-tener-miedo-a-lo-desconocido/
-Visita al laboratorio de alumnos de colegio para orientación vocacional.
Entrevista actividad científica Mujeres científicas. Profesora Katina Onetto y dos alumnas del colegio Simón Bolivar de Las Condes  https://www.youtube.com/watch?v=RZJ1fLeGgRw)
-Entrevista Portal Universidad de Chile por proyectos Fondecyt adjudicados (enero 2018) http://www.uchile.cl/noticias/140417/u-de-chile-adjudico-mas-de-80-proyectos-en-fondecyt-regular-2018


Premios y Distinciones

  •   Distinction to have pulished in a ISI journal of the top 10 in the research area

    UNIVERSIDAD DE CHILE

    Chile, 2013

    Fuentes, P, Pizarro L, Moreno, J.C, Handford M, Rodriguez-Concepción M and Stange, C. “Light-dependent changes in plastid differentiation influence carotenoid gene expression and accumulation in carrot roots”. Plant Molecular Biology, 2012, 79 (1), 47-59.

  •   Distinction to the outstanding scientific labor

    UNIVERSIDAD DE CHILE

    Chile, 2009

    Reconocimiento a la destacada labor científica, Facultad de Ciencias, Universidad de Chile, 2009.

  •   Award to the best undergraduate thesis

    SOCIEDAD DE BIOLOGIA DE CHILE

    Chile, 1996

    Premio a la mejor tesis de pregrado otorgado por la Sociedad de Biología Celular de Chile, 1996.

  •   Award to the best undergraduate thesis

    SOCIEDAD DE BIOLOGIA DE CHILE

    Chile, 1996

    Premio a la mejor tesis de pregrado otorgado por la Sociedad de Biología Celular de Chile, 1996.


 

ConferencePoster (33)

Strategy to increase the sweetness in fruits through the expression of sweet proteins thaumatin and brazzein
Abscisic acid and DcPSY2 modulation during salt stress tolerance in D. carota
Functional characterization of the transcriptional co-factor PAR1 by over-expression in carrot
Functional genomics and molecular studies of non-carotenogenic genes involved in synthesis and accumulation of carotenoids in Daucus carota under dark/light conditions
Light affects plastid differentiation, carotenoid composition and gene profile in carrot roots
The two paralogs for Phytoene Synthase (DcPSY1 & DcPSY2) of Daucus carota produce increments in carotenoids with morphological changes and salts stress tolerance in transgenic carrots
Carrot AREB/ABF and Alfin-like transcription factors bind to Daucus carota phytoene synthase 2 (PSY2) promoter
Characterization of Daucus carota phytoene synthase 2 (PSY2) promoter and identification of AREB/ABF transcription factors that bind to this promoter.
Effect of long-term salinity stress on carotenoid biosynthesis in Solanum lycopersicum fruits
Expression of sorbitol dehydrogenase from grapevine (Vitis vinifera) in Nicotiana tabacum and Arabidopsis thaliana
Heterologous expression of carrot DcPsy1 and DcPsy2 in N. tabacum produce an increase in carotenoids and improved tolerance to salt stress
Over-expression of Daucus carota lycopene β-cyclase gene (DcLCYB1) enhances abiotic stress tolerance by a positive feedback in carotenoid, gibberellin and chlorophyll pathways in Nicotiana tabacum plants
A functional Phytoene synthase 2 gene (DcPSY2) of Daucus carota produces orange transgenic carrot seedlings and promotes salt stress tolerance when expressed in Nicotiana tabacum
Daucus carota lycopene b-cyclase (LCYB1) promotes an increment in plant fitness through positive regulation of carotenoid, gibberellin and chorolophyll genes
Improving β-carotene content in fruits: overexpression of a carotenoid mini pathway in tomato
Light and its role in plastid differentiation and carotenoid accumulation in carrot
Phytoene synthase 2 (DcPSY2) of Daucus carota induces morphological and developmental changes in transgenic carrots
Uso de ingeniería metabólica para el desarrollo de manzanas con altos contenidos de Vitamina A y de antioxidantes: Estandarización de protocolos de regeneración y transformación
Uso de ingeniería metabólica para el desarrollo de s. lycopersicum y m. domestica con elevado contenido de vitamina A y de antioxidantes
DcLcyb2 codes for a functional lycopene b-cyclase involved in the biosynthetic carotenoid pathway of Daucus carota
DcLcyb2 of Daucus carota (carrot) as a metabolic engineering tool to confer high carotenoid content and salt tolerance in transgenic tobacco plants
DcLcyb2 of Daucus carota (carrot) is a biotechnological tool to obtain high carotenoid content in tobacco (Nicotiana tabacum) and kiwi (Actinidia deliciosa)
Metabolic Engineering of carotenoid biosynthesis to improve the nutritional value of fruits
Phytoene synthase genes of Malus domestica codify for functional enzymes that are expressed differentially during fruit ripening
Standardization of in vitro culture of Actinidia deliciosa (kiwi) and its transformation overexpressing a carotenogenic gene of Daucus carota (carrot)
The phytoene synthase 2 gene (DcPSY2) of Daucus carota is regulated by ABA and light and enhances tolerance to NaCl stress when overexpressed in Nicotiana tabacum
Carotenoid composition and carotenogenic gene expression in fuji apple (Malus x domestica cv. Fuji)
Design and functional analysis of vectors to increase the carotenoid content in fruits
Estudio Funcional de los genes DcLCYB1 y DcLCYB2 de Daucus carota
Identification of genes that encode for the enzyme lycopene β-cyclase in Malus domestica and its evaluation by heterologous expression in Escherichia coli
Lycopene β-Cyclase 2 gene silencing alters the biosynthetic carotenoid pathway in Daucus carota (carrot)
Metabolic engineering in carrot (Daucus carota) by overexpression of genes involved in the synthesis of carotenoid metabolic precursors
Phytoene synthase 2 gene (DcPSY2) of Daucus carota is functional in planta and enhances tolerance to NaCl stress when overexpressed in Nicotiana tabacum

Article (29)

Carotenoid Biosynthesis and Plastid Development in Plants: The Role of Light
A de novo transcriptome analysis revealed that photomorphogenic genes are required for carotenoid synthesis in the dark-grown carrot taproot
Agrobacterium tumefaciens-Mediated Stable Transformation of Daucus carota
Boosting carotenoid content in Malus domestica var. Fuji by expressing AtDXR through an Agrobacterium-mediated transformation method
Differential role of the two ?-carotene desaturase paralogs in carrot (Daucus carota): ZDS1 is a functional gene essential for plant development and carotenoid synthesis
Editorial: Illuminating Carotenoid Synthesis and Plastid Transition in Plants
Functional characterisation and in silico modelling of MdPSY2 variants and MdPSY5 phytoene synthases from Malus domestica
Salinity impairs photosynthetic capacity and enhances carotenoid-related gene expression and biosynthesis in tomato (Solanum lycopersicum L. cv. Micro-Tom)
Solanum lycopersicum (tomato) possesses mitochondrial and plastidial lipoyl synthases capable of increasing lipoylation levels when expressed in bacteria
AtA6PR1 and AtA6PR2 encode putative aldose 6-phosphate reductases that are cytosolically localized and respond differentially to cold and salt stress in Arabidopsis thaliana
Identification of Lycopene epsilon cyclase (LCYE) gene mutants to potentially increase ?-carotene content in durum wheat (Triticum turgidum L.ssp. Durum) through TILLING
Unraveling the induction of phytoene synthase 2 expression by salt stress and abscisic acid in Daucus carota
Illuminating colors: regulation of carotenoid biosynthesis and accumulation by light
Metabolic engineering of carotenoid content in Brassica napus L. through seed-specific overexpression of an endogenous PSY gene A
Apocarotenoids: A new carotenoid-derived pathway
Carotenoid biosynthesis in daucus carota
Differential Contribution of the First Two Enzymes of the MEP Pathway to the Supply of Metabolic Precursors for Carotenoid and Chlorophyll Biosynthesis in Carrot (Daucus carota)
Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene ?-cyclase (Dclcyb1) expression
Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling
Biosynthesis of carotenoids in carrot: An underground story comes to light
Levels of Lycopene beta-Cyclase 1 Modulate Carotenoid Gene Expression and Accumulation in Daucus carota
Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana
Light-dependent changes in plastid differentiation influence carotenoid gene expression and accumulation in carrot roots
Superoxide dismutase is a critical enzyme to alleviate oxidative stress in Aloe vera (L.) Burm. plants subjected to water deficit
Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
The N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants
Tobamovirus coat protein CPCg induces an HR-like response in sensitive tobacco plants
Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants
Regeneration of whole plants from apical meristems of Pinus radiata

Proyecto (11)

Generación de nuevos genotipos de manzano con propiedades funcionales especialmente adaptadas para el consumo fresco y procesado”
Growing in the dark: The role of light-inducible genes in carotenoid biosynthesis regulation in carrot storage roots
Unravelling the role of plant lipoyl synthases in partitioning of carbon and tolerance to salt stress.
Characterization of fruit development and identification of potential bioactives compounds in plums, peach and sweet cherry through conventional breading and molecular techniques
Plántulas de kiwi para la producción de un endulzante natural y frutos más dulces bajos en calorías
Sorbitol synthesis and its role in abiotic stress tolerance in non-Rosaceae species
Determination of The Role of Abscisic Acid on The Changes of Polysaccharide Structure Induced By Water Deficit In Plants of Aloe barbadensis Miller (Aloe Vera)
Functional characterization of Daucus carota phytoene synthase genes DcPSY1 and DcPSY2
Evaluación de la ruta de biosintesis de carotenoides en Daucus carota mediante sobreexpresión de los genes dxs, dxr y hdr involucrados en la generación del sustrato inicial ipp (isopentenil pirofosfato)
IBERCAROT, Red Iberoamericana para el estudio de nuevos carotenoides bioactivos como ingredientes de alimentos
Uso de Ingeniería Metabólica para el desarrollo de plántulas de manzano que poseen genes para la síntesis de vitamina a y antioxidantes en los frutos

BookSection (4)

Biosíntesis de Carotenoides en Daucus carota
Functional fruits through metabolic engineering
Biosynthesis of Carotenoids in Plants: Enzymes and Color
“Carotenoids and Photosynthesis - Regulation of Carotenoid Biosyntesis by Photoreceptors

Patent (3)

BINARY VECTOR, GENETIC CONSTRUCTION BASED ON WHOM THAT INCLUDES OPTIMIZED GENES OF THE SYNTHESIS OF CAROTENOID, TRANSFORMED CELLS AND PLANTS
GENETIC CONSTRUCTIONS AND METHOD TO TRANSFORM EFFICIENT PLANTS GENETICALLY SO THAT THEY HAVE BETTER YIELD UNDER CERTAIN CONDITIONS OF ABIOTIC STRESS
Metodo de obtencion de conjugado Acido Abscisico (ABA)-Albumina de Suero Bovino (BSA) sin dialisis, en un tiempo menor que los metodos convencionales y a temperatura ambiente​

BookWhole (1)

Carotenoids in Nature: Biosynthesis, Regulation and Function

Review (1)

Light-dependent regulation of carotenoid biosynthesis in plants
82
Claudia Stange

Titular Professor

Department of Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

8
Michael Handford

Associate Professor

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE BIOLOGÍA

Santiago, Chile

3
Lorena Pizarro

Academino

Escuela de Agronomía y Veterinaria

Universidad de O'Higgins

San Fernando, Israel

2
Ariel Cerda

Estudiante de Doctorado

Departamento de Genética Molecular y Microbiología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Ricardo Cabrera

Assitant Pofessor

Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Rodrigo Contreras

Postdoc

Departamento de Biología

USACH

Santiago, Chile

1
Sergey Kuleshov

Full professor

Departamento de Ciencias Físicas

Universidad Andrés Bello

Santiago, Chile

1
Andrés Schwember

Profesor Asociado

Ciencias Vegetales

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Tomas Perez-Acle

Full Professor

Computational Biology Lab

Fundación Ciencia para la Vida

Santiago, Chile

1
Víctor Cifuentes

Prof. Titular

Departamento de Ciencias Ecológicas

FACULTAD DE CIENCIAS, UNIVERSIDAD DE CHILE.

Santiago, Chile

1
Alejandro Undurraga

Asistente de investigacion

Klein Biotecnologia limitada

Puente Alto, Chile

1
Liliana Cardemil

Profesor Titular

Departamento de Biología

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Jorge Araya

Investigador

Personal

Colina, Chile

1
Alvaro Elorza

ASSOCIATE PROFESSOR

INSTITUTE OF BIOMEDICAL SCIENCES

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
JONATHAN MALDONADO

Postdoc CONICYT

Dpto. Genética Molecular y Microbiología

Pontificia Universidad Católica de Chile

Santiago, Chile