Evaluación de los efectos tempranos inducidos por 11-desoxicorticosterona, a través de receptor de glucocorticoide y mineralocorticoide, sobre la respuesta a estrés asociada a osmorregulación y metabolismo en juveniles de trucha arcoíris (O. mykiss).

Co-dirección de Camila Fernanda Garrido Pino para Título de Ingeniero en Biotecnología (UNAB). “Efectos del cortisol sobre la dinámica entre la accesibilidad de la cromatina y la expresión génica en tejido muscular de trucha arcoíris (Oncorhynchus mykiss)”. Junio, 2024. Director: Dr. Juan Antonio Valdés.

PRESENTACION DE TRABAJOS EN CONGRESOS INTERNACIONALES

31st Conference of European Comparative Endocrinologists. Ancona, Italy, September 2024.
• Oral: “Transcriptomic and epigenomic responses to cortisol in rainbow trout (Oncorhynchus mykiss) skeletal muscle”. D Aravena-Canales, R Zuloaga, K Llanos-Azócar, V Valenzuela, C Gallardo-Escarate, A Molina, JA Valdés.

Latin american & caribbean aquaculture. Ciudad de Panamá, Panamá, April 2023.
• Oral: “Non-coding RNA regulation of thermal stress response in liver and gills on red cusk-eel Genypterus chilensis”. P Dettleff, A Palacios, R Zuloaga, J Aedo, JM Estrada, A Oliva, A Molina, JA Valdés.

9th International Symposium on Aquatic Animal Health. Santiago, Chile, September 2022.
• Oral: “Early regulation of immune-related genes mediated by cortisol in rainbow trout (Oncorhynchus mykiss) gills”. J Aedo, D Aravena-Canales, R Zuloaga, J Valdés, A Molina.
• Oral: “Role of mineralocorticoid and glucocorticoid receptors in teleost somatic growth and stress”. R Zuloaga, J Aedo, D Aravena, A Molina, JA Valdés.
• Oral: “Evaluating high temperature effects on red cusk-eel (Genypterus chilensis) trough gill de novo transcriptome assembly”. P Dettleff, R Zuloaga, M Fuentes, J Aedo, JM Estrada, A Molina, JA Valdés.

14th International congress on the biology of fish. Montpellier, France, July 2022.
• Oral: “Early regulation of corticosteroid receptors and osmoregulation-related genes in rainbow trout gills mediated by membrane-initiated cortisol signaling”. J Aedo, D Aravena-Canales, R Zuloaga, J Valdes, A Molina.
• Oral: “RNA-seq analysis of thermal stress response in black cusk-eel larvae (Genypterus maculatus)”. P Dettleff, R Zuloaga, C Godoy, M Fuentes, A Molina, JA Valdes.
• Poster: “Comparative transcriptomic analysis reveals contrasting features mediated by glucocorticoid and mineralocorticoid receptors in rainbow trout.”. J Valdes, J Aedo, R Zuloaga, D Aravena, Alfredo Molina.

International society for animal genetics. Virtual conference, July 2021.
• Oral: “Thermal stress generates oxidative damage in liver and gills of red cusk-eel (Genypterus chilensis) juvenile”. P Dettleff, R Zuloaga, P Gonzales, M Fuentes, J Aedo, M Estada, A Molina, JA Valdés.

54th Annual conference physiology and pathology of reproduction and simultaneously 46th Joint congress of veterinary and human medicine. Zurich, Switzerland, February 2021.
• Poster: “Thermal stress generate oxidative damage in black cusk-eel embryos (Genypterus maculatus)”. P Dettleff, R Zuloaga, C Godoy, J Palomino, A Molina, JA Valdés.

International plant & animal genome XXVIII. San Diego, USA, January 2020.
• Poster: “Transcriptomic response of rainbow trout skeletal muscle challenged with Piscirickettsia salmonis under stress”. P Dettleff, R Zuloaga, JA Valdés, A Molina.

Latin american & caribbean aquaculture. San Jose, Costa Rica, November 2019.
• Oral: “Daño oxidativo y atrofia en músculo de juveniles de Congrio colorado (Genypterus chilensis) en respuesta a estrés térmico”. Dettleff, R Zuloaga, M Fuentes, P Gonzalez, J Aedo, A Molina, JA Valdés.
• Oral: “Efecto del estrés térmico en hígado de juveniles de Congrio negro (Genypterus maculatus) usando ensamblaje de novo”. Dettleff, R Zuloaga, M Fuentes, P Gonzalez, C Meneses, A Molina, JA Valdés.
• Poster: “Cortisol modify the transcriptomic response to Piscirickettsia salmonis infection in rainbow trout (Oncorhynchus mykiss) myotubes”. P Dettleff, R Zuloaga, M Fuentes, C Aros, C Altamirano, M Bastias-Molina, C Meneses, JA Valdés, A Molina .

Asian-pacific aquaculture. Chennai, India, June 2019.
• Oral: “Effect of thermal stress in gills and muscle of black cusk-eel Genypterus maculatus juvenile”. P Dettleff, M Fuentes, R Zuloaga, J Aedo, A Molina, JA Valdés.
• Oral: “Evaluation of mRNA and thermal stress in unfertilized eggs of red cusk-eel Genypterus chilensis”. P Dettleff, M Fuentes, R Zuloaga, J Aedo, A Molina, JA Valdés.

13th international congress on the biology of fish. Calgary, Alberta, Canada, July 2018.
• Oral: “Role of cortisol-mediated genomic and non-genomic signaling into skeletal muscle growth in teleost fishes”. J Aedo, C Valenzuela, R Zuloaga, S Boltaña, A Molina, JA Valdés.

VI Binational meeting of ecology. Puerto Iguazú, Misiones, Argentina, September 2016.
• Oral: “La surgencia costera induce cambios en el balance energético, expresión génica y vías de señalización involucradas en el crecimiento muscular del pez intermareal Girella laevifrons”. J Pulgar, M Waldisperg, R Zuloaga, K Mendez, JA Valdés, A Molina.

8th International symposium on fish endocrinology. Gothenburg, Sweden, July 2016.
• Oral: “RNA-seq reveals involvement of oxidative stress in the hepatic fibrosis and skeletal muscle atrophy in response to handling stress in the red cusk-eel (Genypterus chilensis)”. J Aedo, F Pozo, V Aballai, B Espinoza, S Naour, R Zuloaga, C Valenzuela, A Molina, JA Valdés.
• Poster: “Evidence for a possible interplay between IL-1? and autophagy in the fine flounder skeletal muscle”. C Valenzuela, R Zuloaga, T Vera, L Mercado, R Avendaño-Herreraa, IE Einarsdottir, BT Björnsson, JA Valdés, A Molina.
• Poster: “Transcriptional response to confinement stress in skeletal muscle of fine flounder (Paralichthys adpersus) determined by RNA-seq”. J Aedo, R Zuloaga, C Valenzuela, P Vizoso, K Mendez, C Meneses, M Bastias-Molina, IE Einarsdottir, BT Björnsson, JA Valdés, A Molina.

17th International conference on deseases of fish and shellfish. Las Palmas, Spain, September 2015.
• Poster: “Effect of chronic stress over the pattern recognition receptors (PRRs) expression in the skeletal muscle of the fine flounder”. C Valenzuela, R Zuloaga, JA Valdés, A Molina.

27th Conference of european comparative endocrinologists. Rennes, France, August 2014.
• Poster: “Skeletal muscle plasticity induced by seasonal acclimatization in carp involves IGF1 signaling implications on ribosomal biogenesis and protein synthesis”. EN Fuentes, R Zuloaga, JA Valdés, A Molina, M Alvarez.
• Poster: “Parallel evolution shaped by contrasting oceanographic conditions in the intertidal fish Girella laevifrons involves intraspeci?c variation in the expression of igf1 and genes related with muscle atrophy and hypertrophy”. EN Fuentes, R Zuloaga, K Méndez, JA Valdés, A Molina, Pulgar.

16th International congress of the world muscle society. Almancil, Algarve, Portugal, October 2011.
• Poster: “IGF-1 induces myostatin expression through differential activation of the signaling pathways PI3K/Akt, Calcineurin/NFAT, ERK/CREB, CaMK-MEF, Smad2/3 during myoblast proliferation and differentiation”. S Flores, R Zuloaga, A Retamales, C Andrade, K Pino, A Molina. JA Valdés.

Gordon conferences in calcium signalling. Waterville, ME, USA. June 2011.
• Poster: “Myostatin inhibits calcium release induced by IGF-1 in skeletal muscle cells”. S Flores, R Zuloaga, A Retamales, K Pino, A Molina, JA Valdés.

PRESENTACIONES EN CONGRESOS NACIONALES

IX Congreso nacional de acuicultura. Coquimbo, Chile, November 2024.
• Oral: “Efectos tempranos de la 11-deoxicorticosterona sobre hepatocitos y células de epitelio branquial de trucha arcoíris (Oncorhynchus mykiss)”. R Zuloaga, J Aedo, A Molina, JA Valdés.

XLIII Congreso de ciencias del mar. Concepción, Chile, May 2024.
• Oral: “11-deoxicorticosterona (DOC) induce cambios transcriptómicos y fisiológicos en branquias e hígado de trucha arcoíris (Oncorhynchus mykiss)”. R Zuloaga, J Aedo, L Ahumada, A Molina, JA Valdés.
• Poster: “Las condiciones de surgencia regulan el transcriptoma del músculo esquelético de un pez intermareal en la Corriente de Humboldt”. K Llanos-Azócar, R Zuloaga, P Dettleff, VM Pulgar, O Varas, C Duarte, M Aldana, A Molina, JA Valdés, J Pulgar.

XVI Reunión anual de ictiología – ICTIO23. Viña del Mar, Chile, July 2023.
• Oral: “Efecto de la surgencia costera sobre la expresión global de genes en el músculo esquelético de Girella laevifrons (Tschudi, 1846)”. K Llanos-Azócar, R Zuloaga, P Dettleff, O Varas, M Aldana, C Duarte, A Molina, JA Valdés, J Pulgar.

Chilean society for cell biology - XXXIV annual meeting. Puerto Varas, Chile, December 2022.
• Poster: “Potential signaling pathways activated by membrane-initiated actions on rainbow trout (Oncorhynchus mykiss) gills”. J Aedo, D Aravena-Canales, R Zuloaga, D Alegría, JA Valdés, A Molina.
• Poster: “Identifications of long noncoding RNAs modulated by Piscirickettsia salmonis in skeletal muscle of rainbow trout (Oncorhynchus mykiss)”. L Ahumada, R Zuloaga, A Molina, and JA Valdés.
• Poster: “Membrane-initiated cortisol action differentially regulate osmoregulation-related genes in gills and skeletal muscle of rainbow trout (Oncorhynchus mykiss)”. R Zuloaga, J Aedo, D Aravena-Canales, A Molina, JA Valdés.

Chilean society for cell biology - XXXIII annual meeting. Puerto Varas, Chile, November 2019.
• Poster: “Effect of thermal stress on larval and juvenile stages of red sea urchin (Loxechinus albus)”. J González, P Dettleff, R Zuloaga, M Fuentes, P González, JM Estrada, A Molina, JA Valdés.
• Poster: “Effect of cortisol on growth-related genes of rainbow trout (Oncorhynchus mykiss) myotubes challenged with Piscirickettsia salmonis”. L Frez-Muñoz, R Zuloaga, P Dettleff, C Altamirano, C Aros, M Fuentes, M Bastías-Molina, C Meneses, JA Valdés, A Molina.

XL Society for biochemistry and molecular biology of chile annual meeting. Puerto Varas, Chile, September 2017.
• Poster: “Transcriptomic modulation of long non-coding RNAs associated with stress response and growth in the skeletal muscle of the fine flounder (Paralichthys adspersus)”. R Farlora, R Zuloaga, JP Donoso, G Nuñez-Acuña, D Valenzuela-Miranda, C Gallardo-Escarate, JE Aedo, C Meneses, JA Valdés, A Molina.
• Oral: “Membrane-initiated cortisol signaling modulates the early expression of metabolism-related genes in the skeletal muscle of rainbow trout (Oncorhynchus mykiss)”. JE Aedo, R Zuloaga, C Meneses, S Boltaña, A Molina, JA Valdés.

Workshop on functional genomics in aquaculture. Concepción, Chile, March 2017.
• Poster: “Transcriptional response to high stocking density stress in the fine flounder (Paralichthys adspersus)”. J Aedo, R Zuloaga, C Valenzuela, JA Valdés, A Molina.

XXXIX Society for biochemistry and molecular biology of chile annual meeting. Puerto Varas, Chile, September 2016.
• Poster: “Handling stress stimulates teleost mitochondrial biogenesis specifically in skeletal muscle”. R Zuloaga, F Pozo, A Molina, JA Valdés.
• Poster: “Differential expression of metabolism-related genes in liver and skeletal muscle of fine flounder (Paralichthys adspersus) under chronic stress”. J Aedo, R Zuloaga, C Valenzuela, JA Valdés, A Molina.
• Poster: “Activation of muscular atrophy signaling pathways in the fine flounder (Paralichthys adspersus) after a V. ordalii infection”. O Almarza, C Valenzuela, R Zuloaga, R Avendaño-Herrera, JA Valdés, A Molina.
• Poster: “Transcriptional dynamics in the Chilean flounder Paralichthys adspersus under fasting and refeeding process”. D Varas, R Zuloaga, K Mendez, M Salazar, A Molina, M Alvarez.
• Poster: “Autophagy activation in the head kidney of the fine flounder (Paralichthys adspersus) under confinement stress”. T Vera, C Valenzuela, R Zuloaga, S Escobar, JA Valdés, A Molina.
• Poster: “Effect of glucocorticoid-induced stress on the growth pathway and muscle atrophy in the fine flounder (Paralichthys adspersus)”. M Fuentes, C Valenzuela, R Zuloaga, JA Valdés, A Molina.

XXXVIII Society for biochemistry and molecular biology of chile annual meeting. Puerto Varas, Chile, September 2015.
• Poster: “Immune response of the skeletal muscle of fine flounder (Paralichthys adspersus, Steindachner 1867)”. C Valenzuela, R Zuloaga, K Cruz, R Avendaño-Herrera, M Poblete-Morales, R Irgang, JA Valdés, A Molina.
• Poster: “Effects of handling stress on the expression of genes involved in energetic metabolism in red-cusk eel (Genypterus chilensis)”. F Pozo, R Zuloaga, A Molina, A Valdés.
• Poster: “Analysis of confinement-stress transcriptomic response on the skeletal muscle of the fine flounder (Paralichthys adspersus)”. J Aedo, K Mendez, P Vizoso, M Bastias, C Meneses, R Zuloaga, C Valenzuela, JA Valdés, A Molina.
• Poster: “Analysis of the transcriptional expression in response to an abiotic natural stress (salinity) in a freshwater fish (Cyprinus carpio)”. J Sepulveda, C Amodeo, I Ramos, M Salazar, R Zuloaga, A Molina, M Alvarez.
• Poster: “Contrasting oceanographic conditions regulate the expression of muscle atrophy and hypertrophy related genes in intertidal fish Girella laevifrons”. R Zuloaga, K Méndez, JA Valdés, J Pulgar, A Molina.

XXXVII Society for biochemistry and molecular biology of chile annual meeting. Puerto Varas, Chile, October 2014.
• Poster: “Effect of Chronic Stress on Growth Hormone (GH)/Insulin like-Growth Factor 1 (IGF1) system in fish skeletal muscle growth”. R Zuloaga, C Valenzuela, EN Fuentes, JM Estrada, JA Valdes, A Molina.
• Poster: “Chronic stress induces atrophy and immune response related gene expression changes in the skeletal muscle of the fine flounder under farming condition”. C Valenzuela, R Zuloaga, EN Fuentes, JM Estrada, JA Valdes, A Molina.
• Poster: “De novo assembly and characterization of fine flounder (Paralichthys adspersus) transcriptome in different nutritional status using next generation sequencing”. K Méndez, R Zuloaga, C Valenzuela, EN Fuentes, JA Valdés, A Orellana, C Meneses, P Vizoso, A Molina..
• Poster: “Skeletal muscle plasticity induced by seasonal acclimatization in carp involves differential expression of rRNA and molecules that epigenetically regulate its synthesis”. EN Fuentes, I Ramos, R Zuloaga, G Nardocci, C Fernandez De La Reguera, N. Simonet, R Fumeron, JA Valdés, A Molina, M Alvarez.

Chilean society for cell biology – XXVII annual meeting. Puerto Varas, Chile, October 2013.
• Poster: “Differential gene expression of the components of the IGF system, myosins, and ribosomal RNAs in skeletal muscle during seasonal acclimatization of carp (Cyprinus carpio)”. R Zuloaga, EN Fuentes, M Álvarez, JA Valdés, A Molina.
• Poster: “De novo assembly and characterization of red conger eel (Genypterus chilensis) transcriptome using next generation sequencing”. J Aedo, J Maldonado, H Silva, R Zuloaga, EN Fuentes, A Molina, JA Valdés.

Chilean society for cell biology - XXVI annual meeting. Puerto Varas, Chile, October 2012.
• Poster: “The transcription factor CREB is activated by IGF-1 to regulate myostatin expression during myoblast differentiation”. R Zuloaga, J Aedo, A Molina, JA Valdés.

Chilean society for cell biology - XXV annual meeting. Puerto Varas, Chile, November 2011.
• Poster: “The transcription factors NFAT, CREB and SMAD2/3 are differentially regulated by Myostatin/IGF-1 during myoblast differentiation”. S Flores, R Zuloaga, A Retamales, A Molina, JA Valdés.