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Daniel Eduardo Gonzalez Reinoso

PROFESOR ASOCIADO

UNIVERSIDAD DE TALCA

Talca, Chile

Líneas de Investigación


Fisiología, Fisiopatología y Farmacología cardiovascular. Cardiomiopatías: envejecimiento, cardiomiopatía diabética y distrofica. Equilibrio nitroso-redox cardiaco: NADPH oxidasas, óxido nítrico sintasas, S-nitrosoglutation reductasa.

Educación

  •  Ciencias Fisiologicas, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2005
  •  BIOQUIMICA, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 1998

Experiencia Académica

  •   PROFESOR ASOCIADO Full Time

    UNIVERSIDAD DE TALCA

    CIENCIAS DE LA SALUD

    TALCA, Chile

    2010 - A la fecha

  •   RESEARCH FELLOW Full Time

    JOHNS HOPKINS UNIVERSITY

    MEDICAL SCHOOL

    BALTIMORE, Estados Unidos

    2006 - 2007

  •   RESEARCH FELLOW Full Time

    UNIVERSITY OF MIAMI

    MEDICAL SCHOOL

    MIAMI, Estados Unidos

    2007 - 2010

Formación de Capital Humano


FORMACIÓN DE CAPITAL HUMANO

Tutor de los siguientes alumnos, para obtención de títulos profesionales y grados académicos:

Doctorado

Alejandra Vielma. Candidata a Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas, Pontificia Universidad Católica de Chile: Tesis: Rol de NADPH oxidasa (NOX2) en la oxidación de hemicanales de conexina 43 y su relación con la cardiomiopatía en la distrofia muscular de Duchenne”.

Adriana Victoria Treuer Moya. Candidata a Doctor en Ciencias Mención Ciencias mención Investigación y Desarrollo de Productos Bioactivos. Universidad de Talca NADPH oxidasa NOX2, como blanco para el desarrollo de inhibidores específicos-selectivos, mediante la síntesis asistida de derivados de heteroaril-acrilonitrilos .

Roberto Antonio Fuentealba Leyton. Candidato a Doctor en Ciencias Mención Ciencias mención Investigación y Desarrollo de Productos Bioactivos. Universidad de Talca. Título tesis: Acrilonitrilos como inhibidores de NADPH oxidasa cardiaca.

María José Rodríguez Becerra. Candidata a Doctor en Ciencias Mención Ciencias mención Investigación y Desarrollo de Productos Bioactivos. Universidad de Talca. Título tesis: Resolvinas (RvE1) y Maresinas (MaR1) como agentes hepatoprotectores en un modelo de fibrosis.

Magister

Karen De Los Ángeles Soto Orellana. Título tesis: Impacto de tetrahidrobopterina (BH4) sobre parámetros bioquímicos y morfológicos de cardiomiopatía diabética.

Oscar Arriagada. Título tesis: Evaluación del efecto cardioprotector de la inhibición de la S –nitrosoglutatión reductasa en modelo de isquemia reperfusión

Pregrado

Gustavo Vásquez H. Memoria para optar al título de Cirujano Dentista, Universidad de Talca “Determinación del rol de las especies reactivas de oxigeno (ROS) en la contractilidad cardiaca”. (2011)

Claudia Pareja, Practica Profesional para obtener el título de Ingeniero en Bioinformatica, Universidad de Talca: “Estudio de Interacciones Proteína-Ligando en sub-unidades citosolicas de la Familia NOX”. (2012)

Bryan Moreno, Seminario profesional para obtener el grado de Licenciado en de Tecnología Médica, Universidad de Talca: “Efecto de una dieta rica en nitratos en un modelo animal de síndrome metabólico” (2012)

Karla Valdes Adriazola. Tecnólogo Medico: “Óxido Nítrico Sintasa Endotelial en Ratones Deficientes en ApoE. (2013)

Vanessa Cavieres Alvarez. Tecnólogo Medico Universidad de Talca: “Estudio de la Reactividad Vascular en Aortas de Ratones Deficientes en ApoE” (2013)

Gonzalo Faúndez Luna, Tecnólogo Medico, Universidad de Talca: Contractilidad del miocito cardiaco: rol de NOS. (2014)

Guillermo Barrios Barrios, Tecnólogo Medico, Universidad de Talca: Efecto del Envejecimiento en el Acoplamiento Excitación-Contracción en Ratas. (2015)

Sergio Saldivia Gailard, Tecnologo Medico, Universidad de Talca. Determinación de nox2 y nox4 en el miocardio de ratas, analizando su relación con la cardiomiopatía diabética.(2015)

Diego Arauna. Influencia de niveles de BH4 en la fisiopatología de la miocardiopatía diabética. Memoria para optar al grado de licenciado en Tecnología Médica. (2015)

Claudia Sánchez. La inhibición farmacológica de la NADPH oxidasa reduce la fibrosis cardíaca en un modelo animal de distrofia de Duchenne. Memoria para optar al grado de Licenciado en Tecnología Médica. (2015)

Madelaine Núñez Terán. Análisis de apoptosis en el corazón de ratas diabéticas sometidas a entrenamiento físico regular. Memoria para optar al grado de licenciado en Tecnología Médica. (2016)

Natalia Garcia Manriquez. Evaluacion de la autofagia en el miocardio de ratones con distrofia muscular de Duchenne .Memoria para optar al grado de licenciado en Tecnología Médica. (2016)

Nikol Ponce Rojas. Tecnología Médica. Memoria: Evaluación de la expresión de proteínas del acoplamiento excitación-contracción SERCA2, fosfolamban, fosfolamban serina 16 y conexina 43, además de NADPH oxidasa en corazón de rata envejecida. (2016)


Difusión y Transferencia


COMUNICACIONES PRESENTADAS EN REUNIONES CIENTÍFICAS (SELECCIÓN)

Alejandra Z Vielma, Mauricio P Boric, and Daniel R González. NOX2 activity induces lateralization, S-nitrosylation and opening of connexin/pannexin hemichannels causing arhythmogenesis and apoptosis in dystrophic cardiomyopathy. International Society for Heart Research, Buenos Aires, Argentina, 2016.

Adriana Treuer, Margarita Gutierrez, Roberto Ebensperger, Mario Faundez, Daniel R Gonzalez. A Novel Acrylonitrile Compound as Inhibitor of NADPH Oxidase 2. Pharmacology 2015. Londres, Inglaterra .

Barrios, G.; Gonzalez, DR. The effect of aging on calcium transients in rat cardiomyocytes: impact of NOX inhibition. Biophysical Society 59th annual Meeting, Baltimore, Estados Unidos 07/02/20-11/02/2015

Gonzalez, DR.;Treuer, AV.; Dulce, RA.: NOS-1 Uncoupling in Dystrophic Cardiomyopathy. PANAM - 1st PanAmerican Congress of Physiological Sciences. Foz de Iguazu, BRASIL.

Adriana V Treuer, Raúl A Dulce, Daniel R González NOX2 Inhibition Restores Contractility, Intracellular Calcium Handling and Reduces Arrhythmogenicity in Dystrophic Cardiomyopathy British Pharmacological Society, Pharmacology, December 2013, London.

Ponencias
? Conferencia: Nitroso-redox imbalance in the cardiac myocyte. Daniel R. Gonzalez. XXXI REUNION ANUAL DE LA SOCIEDAD CHILENA DE CIENCIAS FISIOLÓGICAS realizada en el Hotel Nothofagus de la Reserva Biológica Huilo-Huilo, Región de Los Ríos, entre el 6 y 9 de Septiembre de 2016.

? Chronic exercise reduces fibrosis and hypertrophy but not oxidative stress in diabetic cardiomyopathy. BIT´s 7th Annual International Congress of Cardiology- 2015, Shanghai, China.

? El Reacoplamiento de NOS1 en la Cardiomiopatia Distrofica Restaura el manejo del Ca2+ intracelular. I Reunión de Investigación e Innovación en Salud Vascular. Universidad del Bio-Bio, Chillan, 30 de Abril 2015

? Cardiomiopatia en la enfermedad de Duchenne. Simposio "Inflamación, Transiciones en Biomedicina, de cáncer a enfermedad cardiovascular y neurobiología". 27 de Diciembre de 2013 Universidad Católica del Maule.



 

Article (55)

Effects of Phaseolus vulgaris Extract on Lipolytic Activity and Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes: A Strategy to Prevent Obesity
NOX Inhibition Improves beta-Adrenergic Stimulated Contractility and Intracellular Calcium Handling in the Aged Rat Heart
High-Intensity Exercise Reduces Cardiac Fibrosis and Hypertrophy but Does Not Restore the Nitroso-Redox Imbalance in Diabetic Cardiomyopathy
Simplified HPLC methodology for quantifying biological pterins by selective oxidation
Simplified HPLC methodology for quantifying biological pterins by selective oxidation.
Nitric Oxide Synthase 1 Modulates Basal and ?-Adrenergic-Stimulated Contractility by Rapid and Reversible Redox-Dependent S-Nitrosylation of the Heart
Nitric oxide synthase 1 modulates basal and beta adrenergic-stimulated contractility by rapid and reversible redox-dependent S-nitrosylation of the heart
Nitrate consumption and potential beneficial effect on cardiovascular health
Nitric oxide synthases, S-nitrosylation and cardiovascular health: from molecular mechanisms to therapeutic opportunities (review).
NADPH oxidase-2 inhibition restores contractility and intracellular calcium handling and reduces arrhythmogenicity in dystrophic cardiomyopathy
NADPH oxidase-2 inhibition restores contractility and intracellular calcium handling and reduces arrhythmogenicity in dystrophic cardiomyopathy.
NOS1AP modulates intracellular Ca(2+) in cardiac myocytes and is up-regulated in dystrophic cardiomyopathy.
Vascular hypercontractility and endothelial dysfunction before development of atherosclerosis in moderate dyslipidemia: role for nitric oxide and interleukin-6.
Vascular hypercontractility and endothelial dysfunction before development of atherosclerosis in moderatedyslipidemia: Role for nitric oxide and interleukin-6
Coordinated Endothelial Nitric Oxide Synthase Activation by Translocation and Phosphorylation Determines Flow-Induced Nitric Oxide Production in Resistance Vessels
Coordinated endothelial nitric oxide synthase activation by translocation and phosphorylation determines flow-induced nitric oxide production in resistance vessels.
Hydralazine and Organic Nitrates Restore Impaired Excitation-Contraction Coupling by Reducing Calcium Leak Associated with Nitroso-Redox Imbalance
Hydralazine and organic nitrates restore impaired excitation-contraction coupling by reducing calcium leak associated with nitroso-redox imbalance.
β2-Adrenergic Receptor-Coupled Phosphoinositide 3-Kinase Constrains cAMP-Dependent Increases in Cardiac Inotropy Through Phosphodiesterase 4 Activation
Activation of growth hormone releasing hormone (GHRH) receptor stimulates cardiac reverse remodeling after myocardial infarction (MI)
Activation of growth hormone releasing hormone (GHRH) receptor stimulates cardiac reverse remodeling after myocardial infarction (MI).
Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function
Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function.
Fractions of aqueous and methanolic extracts from tomato (Solanum lycopersicum L.) present platelet antiaggregant activity
Fractions of aqueous and methanolic extracts from tomato (Solanum lycopersicum L.) present platelet antiaggregant activity.
Gene expression of adipose tissue, endothelial cells and platelets in subjects with metabolic syndrome (Review).
Inhibition of human topoisomerase I and activation of caspase-3 by aza-angucyclinones and arylaminopyrimido[4,5-c]isoquinoline-7,10-quinones
Inhibition of human topoisomerase I and activation of caspase-3 by aza-angucyclinones and arylaminopyrimido[4,5-c]isoquinoline-7,10-quinones.
Elevated concentration of asymmetric dimethylarginine (ADMA) in individuals with metabolic syndrome
Elevated concentration of asymmetric dimethylarginine (ADMA) in individuals with metabolic syndrome.
Impaired S -Nitrosylation of the Ryanodine Receptor Caused by Xanthine Oxidase Activity Contributes to Calcium Leak in Heart Failure
Impaired S-nitrosylation of the ryanodine receptor caused by xanthine oxidase activity contributes to calcium leak in heart failure.
Physical activity reduces circulating TNF-alpha but not pro-thrombotic factors levels in patients with metabolic syndrome
Physical activity reduces circulating TNF-alpha but not pro-thrombotic factors levels in patients with metabolic syndrome
β2-adrenergic receptor-coupled phosphoinositide 3-kinase constrains cAMP-dependent increases in cardiac inotropy through phosphodiesterase 4 activation.
Neuronal nitric oxide synthase in heart mitochondria: a matter of life or death
Neuronal nitric oxide synthase in heart mitochondria: a matter of life or death.
S-Nitrosylation of Cardiac Ion Channels
S-Nitrosylation of cardiac ion channels.
Differential role of S-nitrosylation and the NO-cGMP-PKG pathway in cardiac contractility
Differential role of S-nitrosylation and the NO-cGMP-PKG pathway in cardiac contractility.
Deficient ryanodine receptor S-nitrosylation increases sarcoplasmic reticulum calcium leak and arrhythmogenesis in cardiomyocytes
Deficient ryanodine receptor S-nitrosylation increases sarcoplasmic reticulum calcium leak and arrhythmogenesis in cardiomyocytes.
Reduced neuronal nitric oxide synthase expression contributes to cardiac oxidative stress and nitroso-redox imbalance in ob/ob mice
Reduced neuronal nitric oxide synthase expression contributes to cardiac oxidative stress and nitroso-redox imbalance in ob/ob mice.
Leptin repletion restores depressed {beta}-adrenergic contractility in ob/ob mice independently of cardiac hypertrophy.
Leptin repletion restores depressed β-adrenergic contractility in ob/ob mice independently of cardiac hypertrophy
Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation-contraction coupling
Neuronal nitric oxide synthase negatively regulates xanthine oxidoreductase inhibition of cardiac excitation-contraction coupling.
ACh-induced endothelial NO synthase translocation, NO release and vasodilatation in the hamster microcirculation in vivo
ACh-induced endothelial NO synthase translocation, NO release and vasodilatation in the hamster microcirculation in vivo.
In vivo assessment of microvascular nitric oxide production and its relation with blood flow
In vivo assessment of microvascular nitric oxide production and its relation with blood flow.
Stimulation of NO production and of eNOS phosphorylation in the microcirculation in vivo
Stimulation of NO production and of eNOS phosphorylation in the microcirculation in vivo.

Abstract (15)

The Effect of Aging on Calcium Transients in Rat Cardiomyocytes: Impact of NOX Inhibition
NOS1AP Modulates Intracellular Ca2+ in Cardiac Myocytes and is Up-Regulated in Dystrophic Cardiomyopathy
Interaction between eNOS translocation and phosphorylation in flow -induced NO production
NOS1-dependent S-nitrosylation of cardiac calcium-handling proteins (RyR2, SERCA, and L-type Ca2+channel) modulates basal contractility and adrenergic inotropism
Beta-adrenergic stimulation causes rapid NOS1-dependent S-nitrosylation of cardiac proteins, associated with increased contractility
NOX2 Inhibition Restores Contractility, Intracellular Calcium Handling and Reduces Arrhythmogenicity in Dystrophic Cardiomyopathy
Convergence of flow and bradykinin-induced NO production in intact resistance vessels
Nitric oxide increases or decreases cardiac contractility depending on concentration and signaling pathways
Influence of gender and estradiol on microvascular nitric oxide production
Effects of PAF on subcellular eNOS distribution in the hamster microcirculation in vivo. Interactions with caveolin-1 and HSP-90.
Mechanisms of flow-induced nitric oxide production in resistance arteries: The renovascular hypertension case.
Acetylcholine induces nitric oxide release and subcellular eNOS translocation in the hamster microcirculation in vivo
Acetylcholine induces nitric oxide release and subcellular eNOS translocation preceding blood now increase in the hamster microcirculation in vivo
Effects of flow and calcium on nitric oxide release and eNOS translocation in intact rat resistance arteries
Nitric oxide production and subcellular translocation of eNOS induced by now changes in intact rat resistance arteries

Actualizacion (1)

EL CONSUMO DE FRUTAS Y HORTALIZAS AYUDA A PREVENIR EL DAÑO ENDOTELIAL

Review (3)

El consumo de nitrato y su potencial efecto benéfico sobre la salud cardiovascular
Nitric oxide synthases, S-nitrosylation and cardiovascular health: From molecular mechanisms to therapeutic opportunities
Gene expression of adipose tissue, endothelial cells and platelets in subjects with metabolic syndrome (Review)
45
Daniel Gonzalez

PROFESOR ASOCIADO

CIENCIAS BASICAS BIOMEDICAS

UNIVERSIDAD DE TALCA

Talca, Chile

7
Iván Palomo

Hematology Professor

Clinical Biochemistry and Immunohematology

UNIVERSIDAD DE TALCA

Talca, Chile

5
Rodrigo Moore

Profesor Asociado

Bioquímica Clínica e Inmunohematología

UNIVERSIDAD DE TALCA

Talca, Chile

4
Xavier Figueroa

Associate Professor

Departamento de Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Eduardo Fuentes

Profesor Asociado

Universidad de Talca

Talca, Chile

2
Marcelo Alarcón

Profesor

Bioquimica Clinica e Inmunahematologia

UNIVERSIDAD DE TALCA

Talca, Chile

1
Alejandra Vielma

Post-doctorado

Ciencias Biológicas, Ciencias Fisiológicas

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Daniel Rojas

Profesor

Universidad Diego Portales

Santiago, Chile

1
Luis Guzman

Profesor Asistente

Departamento Bioquímica Clínica e Inmunohematología

UNIVERSIDAD DE TALCA

Talca, Chile

1
Adriana Treuer

Postdoctoral Researcher

BASIC BIOMEDICAL SCIENCES

Universidad de Talca

Talca, Chile

1
Cristian Valdés

Posdoctorado

Vicerrectoría de Investigación

Universidad Católica del Maule

Talca, Chile

1
David Vásquez

Profesor Asistente

Química Farmacológica y Toxicológica

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Diego Arauna

Investigador Postdoctorante

Universidad de Talca

Talca, Chile

1
Juana Ibacache

académico

Ciencias del ambiente

Universidad de Santiago de Chile

Santiago, Chile

1
margarita gutierrez

Proffesor

UNIVERSIDAD DE TALCA

Talca, Chile

1
PABLO PEREZ

Investigador Postdoctoral

Anatomía Patológica/Oncología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
luis Urzua

DIRECTOR DE CARRERA

SALUD

Universidad Santo Tomás

Talca, Chile