Man

Alejandro Mauricio Reyes Pinto

Profesor Titular

Universidad Austral de Chile

Valdivia, Chile

Líneas de Investigación


Structure - functional biology of membrane transporters, including glucose and vitamin C transporters Protein engineering

Educación

  •  Biology, Universidad de Chile. Chile, 1982
  •  Doctor in Sciences, Universidad de Chile. Chile, 1990

Experiencia Académica

  •   Ayudante Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Ciencias

    Valdivia, Chile

    1983 - 1987

  •   Instructor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Ciencias

    Valdivia, Chile

    1987 - 1990

  •   Auxiliar Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Ciencias

    Valdivia, Chile

    1990 - 1996

  •   Associate Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Ciencias

    Valdivia, Chile

    1996 - 1999

  •   Full Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Ciencias

    Valdivia, Chile

    1999 - At present

  •   Faculty research Investigator Full Time

    University of Missouri - Kansas City

    Biological Sciences

    Kansas City, MO, Estados Unidos

    1990 - 1993

  •   Visiting Research Investigator Full Time

    Memorial Sloan Kettering Cancer Center

    Nueva York, Estados Unidos

    1995 - 1995

  •   Visiting Research Investigator Full Time

    Memorial Sloan Kettering Cancer Center

    Nueva York, Estados Unidos

    1998 - 1998

Experiencia Profesional

  •   Principal Investigator FONDECYT 1981018

    Universidad Austral de Chile

    Valdivia, Chile

    1998 - 2001

  •   Principal Investigator FONDECYT 1020908

    Universidad Austral de Chile

    Valdivia, Chile

    2002 - 2006

  •   Principal Investigator FONDECYT 1060198

    Universidad Austral de Chile

    Valdivia, Chile

    2006 - 2010

  •   Director FONDEF D07I1117

    Universidad Austral de Chile

    Valdivia, Chile

    2009 - 2013

  •   Coinvestigator FONDEF CA12I10022

    Universidad Austral de Chile

    Valdivia, Chile

    2013 - 2015

Formación de Capital Humano


I have directed 8 undergraduate Thesis and 1 Doctoral thesis in the last 3 years. I also participates in teaching of undergraduate students in regular courses of General Biochemistry for students of the schools of Biochemistry, Medicine and Medical Technology. I am Responsible Professor of the course of Introduction to Protein Engineering and Design, for Biochemistry students. I am also Responsible of the course Genimocs and Proteomics for undergraduates students of the school of Biochemistry. I am Responsible Professor of the course of Protein Structure and Proteomics for graduate students on the PhD Program of the Faculty of Sciences. I also participates in the experimental training of at least 2 undergraduate Biochemistry or Licentiate of Sciences students per semester; each one develops an individual research project in the laboratory. I also participates in expreimental training of graduate students that rotations in my laboratory.


Difusión y Transferencia


I have participated on a regular basis on program of diffusion of basic and applied sciences in schools and colleges of primary and secondary educational level, in Valdivia and Concepción. I also present and discuss the results of our project at least once a year as conferences in the Programa Explora “1000 científicos, 1000 aulas” or in talks to students of the school of our Faculty of sciences. On these activities, I actively promotes the participation of students from the secondary education level or undergraduate students in sciences and in intramural research project. In the last three years I have also participated as evaluator of the project presented for students in the Regional Explora congress.


Premios y Distinciones

  •   Doctoral Research Fellowships

    FUNDACION ANDES

    Chile, 1988

    Fellowships to develop a 3-month international research training in an advanced field in basic sciences as part of a graduate program. The training was develop in protein chemistry in the University of Missouri-Kansas City, MO, USA.

  •   PostDoctoral Fellowship

    University of Missouri-Kansas City

    Estados Unidos, 1990

    3-years Postdoctoral Training in protein chemistry and folding

  •   Visiting Research Investigator

    MEMORIAL SLOAN-KETTERING CANCER CENTER

    Estados Unidos, 1995

    2 month training in cell biology and experimental methods to monitor substrate cellular uptake

  •   Visiting Research Investigator

    MEMORIAL SLOAN-KETTERING CANCER CENTER

    Chile, 1998

    4 month training in cellular and molecular physiology of vitamin C


 

Article (22)

Cis-regulatory elements involved in species-specific transcriptional regulation of the SVCT1 gene in rat and human hepatoma cells

Muñoz, Alejandra; Villagrán, Marcelo; Guzmán, Paula; Solíz, Carlos; Gatica, Marcell; Aylwin, Carlos; Sweet, Karen; Maldonado, Mafalda; Escobar, Elizabeth; Reyes, Alejandro M.; Toledo, Jorge R.; Sánchez Oliberto; Oñate, Sergio A.; Carlos Vera, Juan; RIVAS, Coralia I.

Article ISI SCOPUS
Free Radical Biology and Medicine  (2015)

Mitochondrial ascorbic acid transport is mediated by a low-affinity form of the sodium-coupled ascorbic acid transporter-2

Muñoz-Montesino C, Roa FJ, Peña E, González M, Sotomayor K, Inostroza E, Muñoz CA, González I, Maldonado M, Soliz C, Reyes AM, Vera JC, Rivas CI

Article ISI SCOPUS
FREE RADICAL BIOLOGY AND MEDICINE  (2014)

Resolution of the direct interaction with and inhibition of the human GLUT1 hexose transporter by resveratrol from its effect on glucose accumulation

Salas, M; Obando, P; Ojeda L.; Ojeda P.; Pérez, A.; Vargas-Uribe, M; Rivas, CI; Vera, JC; Reyes, AM

Article ISI SCOPUS
American Journal of Physiology - Cell Physiology  (2013)

Caracterización de enzimas hidrolíticas de Aspergillus ficuum producidas en fermentación sólida sobre torta de canola

Costa, Marcia; Torres, Marcelo; Reyes, Alejandro

Article
Revista Colombiana de Biotecnología  (2012)

Essential role of intracellular glutathione in controlling ascorbic acid transporter expression and function in rat hepatocytes and hepatoma cells

Mardones L.; Zuñiga F.A.; Villagrán M.; Sotomayor, K; Mendoza, P; Escobar, D.; Gonzalez, M.; Ormazabal, V; Maldonado, M; Onate, G; Angulo, C; Concha I.I.; Reyes A.M.; Cárcamo J.G.; Barra V; Vera, J. C.; Rivas C.I.

Article ISI SCOPUS
Free Radical Biology and Medicine  (2012)

Noncompetitive blocking of human GLUT1 hexose transporter by methylxanthines reveals an exofacial regulatory binding site

Ojeda P.; Pérez, A.; Ojeda L.; Vargas-Uribe, M; Rivas C.I.; Salas, M; Vera, J. C.; Reyes A.M.

Article ISI SCOPUS
American Journal of Physiology - Cell Physiology  (2012)

Hexose Transporter GLUT1 Harbors Several Distinct Regulatory Binding Sites for Flavones and Tyrphostins

Pérez, A.; Ojeda P.; Ojeda L.; Salas, M; Rivas, CI; Vera, JC; Reyes, AM

Article ISI SCOPUS
BIOCHEMISTRY  (2011)

Histidine Residues in the Na+-coupled Ascorbic Acid Transporter-2 (SVCT2) Are Central Regulators of SVCT2 Function, Modulating pH Sensitivity, Transporter Kinetics, Na+ Cooperativity, Conformational Stability, and Subcellular Localization

Ormazabal, V; Zuniga, FA; Escobar, E; Aylwin, C; Salas Burgos A.; Godoy A.; Reyes, AM; Vera, JC; Rivas, CI

Article ISI SCOPUS
Journal of Biological Chemistry  (2010)

Producción y purificación parcial de enzimas hidrolíticas de Aspergillus ficuum en fermentación sólida sobre residuos agroindustriales

Costa, Marcia; Torres, Marcelo; Magariños, Haroldo; Alejandro Reyes

Article
Revista Colombiana de Biotecnología  (2010)

Endofacial competitive inhibition of the glucose transporter 1 activity by gossypol

Pérez, A.; Ojeda P.; Valenzuela, X; Ortega M.; Sánchez C; Ojeda L.; Castro M.; Carcamo, JG; Rauch MC; Concha, II; Rivas, CI; Vera, JC; Reyes, AM

Article ISI SCOPUS
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY  (2009)

Mechanistic insights and functional determinants of the transport cycle of the ascorbic acid transporter SVCT2

Godoy A.; Ormazabal, V; Moraga-Cid, G; Zuniga, FA; Sotomayor P.; Barra V; Vásquez O.; Montecinos, V.; Mardones L.; Guzmán C; Villagrán M.; Aguayo, LG; Onate, SA; Reyes, AM; Carcamo, JG; Rivas, CI; Vera, JC

Article ISI SCOPUS
JOURNAL OF BIOLOGICAL CHEMISTRY  (2007)

Redefining the facilitated transport of mannose in human cells: Absence of a glucose-insensitive, high-affinity facilitated mannose transport system

Rodríguez, P.; Rivas, CI; Godoy A.; Villanueva, M.; Fischbarg, J; Vera, JC; Reyes, AM

Article ISI SCOPUS
BIOCHEMISTRY  (2005)

Nativelike intermediate on the unfolding pathway of pig kidney fructose-1,6-bisphosphatase

Reyes, AM; Ludwig, HC; Yanez, AJ; Rodriguez, PH; Slebe, JC

Article ISI SCOPUS
BIOCHEMISTRY  (2003)

Nicotinamide is not a substrate of the facilitative hexose transporter GLUT1

Reyes A.M.; Bustamante F.; Ortega M.; Rivas C.I.; Vera, J. C.; Donnet, C; Rossi J.P.; Fischbarg, J

Article
BIOCHEMISTRY  (2002)

Direct inhibition of the hexose transporter GLUT1 by tyrosine kinase inhibitors

Vera, JC; Reyes, AM; Velasquez, FV; Rivas, CI; Zhang, RH; Strobel P.; Slebe, JC; Nunez-Alarcon, J; Golde, DW

Article ISI SCOPUS
BIOCHEMISTRY  (2001)

The C1-C2 interface residue lysine 50 of pig kidney fructose-1,6-bisphosphatase has a crucial role in the cooperative signal transmission of the AMP inhibition

Carcamo, JG; Yanez, AJ; Ludwig, HC; Leon, O.; Pinto, RO; Reyes, AM; Slebe, JC

Article ISI SCOPUS
EUROPEAN JOURNAL OF BIOCHEMISTRY  (2000)

Suppression of kinetic AMP cooperativity of fructose-1,6-bisphosphatase by carbamoylation of lysine 50

Ludwig H.C.; Herrera R.; Reyes A.M.; Hubert E.; Slebe J.C

Article
Journal of Protein Chemistry  (1999)

Suppression of kinetic AMP cooperativity of fructose1,6-bisphosphatase by carbamoylation of lysine 50

Ludwig H.C.; Siebe J.C.; Herrera R.; Reyes A.M.; Hubert E.

Article
PROTEIN JOURNAL  (1999)

Hexose transporter expression and function in mammalian spermatozoa: Cellular localization and transport of hexoses and vitamin C

Angulo, C; Rauch M.C.; Droppelmann A.; Reyes A.M.; Slebe J.C; Concha I.I.; Delgado-Lopez, F; Guaiquil V.H.; Vera, J. C.

Article
JOURNAL OF CELLULAR BIOCHEMISTRY  (1998)

Human erythrocytes express GLUT5 and transport fructose

Concha I.I.; Velasquez F.V.; Martínez J.M.; Angulo, C; Droppelmann A.; Reyes A.M.; Slebe J.C; Vera, J. C.; Golde, D. W.

Article
BLOOD  (1997)

Genistein is a natural inhibitor of hexose and dehydroascorbic acid transport through the glucose transporter, GLUT1

Vera, J. C.; Velasquez F.V.; Rivas C.I.; Zhang R.H.; Golde, D. W.; Scher H.I.; Reyes A.M.; Cárcamo J.G.; Strobel P.; Iribarren R.; Siebe J.C.

Article
JOURNAL OF BIOLOGICAL CHEMISTRY  (1996)

NICOTINARNIDE IS NOT A SUBSTRATE OF THE FACILITATIVE HEXOSE TRANSPORTER GLUT1

Reyes, Alejandro; Bustamante, Fernando; Rivas, Coralia; Ortega, Marcela; Donnet, Claudia; Rossi, Juan; Fischbarg, Jorge

Article
BIOCHEMISTRY  

Abstract (3)

New insights into the functional characteristic of GLUT12: Na+/Glucose cotransport and glucose induced outward chloride currents

Pujol-Gimenez, J; Pérez, A.; Reyes A.; Lostao, MP

Abstract ISI
FEBS JOURNAL  (2012)

Electrogenic characteristics of the facilitative glucose transporter GLUT12

Lostao, MP; Pérez, A.; Reyes A.; Pujol, J

Abstract ISI
FASEB JOURNAL  (2011)

Identification of regulatory binding sites on the Glut1 glucose transporter

Reyes A.; Ojeda P.; Pérez, A.; Valenzuela, X; Vera, JC

Abstract ISI
FASEB JOURNAL  (2010)

Proyecto (3)

Development of a phytase enzyme preparation active at low temperatures (10-16 ° C) to supplement "in vivo" fish diets with high levels of plant proteins

Costa, Marcia; Reyes, Alejandro M.

Proyecto
 (0)

Identification, development and validation of new inhibitory molecules with selectivity by glucose transporters for their application in the control of hyperglycemia characteristics of diabetes mellitus, obesity and metabolic syndrome

Reyes, Alejandro M.; Vera, Juan C.

Proyecto
 (0)

Structure-functional biology of GLUT1 and GLUT2 human hexose transporters

Reyes, Alejandro M.

Proyecto
 (0)

34
Alejandro Reyes

Profesor Titular

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

26
CORALIA RIVAS

Associated Professor

Pathophysiology

UNIVERSIDAD DE CONCEPCION

CONCEPCION, Chile

16
Juan Vera

Full professor

Pathophysiology

University of Concepcion

Concepción, Chile

6
Juan Carcamo

Profesor Titular

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

5
Paola Ojeda

Profesor Adjunto

Ciencias Pre-clinicas

Universidad Católica del Maule

Talca, Chile

3
Alejandro Godoy

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

3
MARCELO VILLAGRAN

Profesor asociado

Departamento de Ciencias Básicas

Universidad Catolica de la Santísima Concepción

Concepcion, Chile

3
Felipe Zuñiga

Profesor Asistente

Bioquimica Clinica e Inmunologia

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

3
Valeska Ormazabal

Profesor asistente

Farmacología

Universidad de Concepción

Concepción, Chile

3
Alexis Salas

Assistant Professor

Pharmacology

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

2
Lorena Mardones

ASSOCIATED PROFESSOR

BASIC SCIENCES AND MORPHOLOGY

UNIVERSIDAD CATOLICA DE LA SANTISIMA CONCEPCION

CONCEPCION, Chile

2
Ilona Concha

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

2
mauricio gonzalez

phd

fisiopatologia

universidad de concepción

concepcion, Chile

2
Ximena Valenzuela

Académico

Campus Patagonia

Universidad Austral de Chile

Coyhaique, Chile

2
Maria Escobar

Postdoctorado

Biología Molecular Vegetal

Universidad de Chile

Santiago , Chile

2
Sergio Onate

Associate Professor

Medical Specialties

UNIVERSIDAD DE CONCEPCION, DEPARTAMENTO FISIOPATOLOGIA

concepcion, Chile

2
Viviana Montecinos

Assistant Professor

Hematology Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

2
Mauricio Vargas

Head of R&D

R&D

Kura Biotech

Puerto Varas, Chile

2
Luis Aguayo

Full Professor

Department of Physiology

Universidad de Concepcion

Concepcion, Chile

2
Alejandro Yanez

Full professor

Faculty of Science

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Paula Sotomayor

Investigador

Medicina

Pontificia Universidad Católica de Chile

Santiago, Chile

1
PAULA GUZMÁN

Profesional Bioquímico

Laboratorio Clínico

Hospital Clínico Regional de Concepción Guillermo Grant Benavente

Concepción, Chile

1
Jorge Toledo

Director de Departamento

Fisiopatología

Universidad de Concepción

Concepcion, Chile

1
Oliberto Sanchez

Associate Professor

Pharmacology

University of Concepción

Concepción, Chile

1
Fernando Delgado

Associate Professor

Division of Medicine

Universidad Catolica del Maule

Talca, Chile

1
Pablo Strobel

Prrofesional de laboratorio

Facultad Ciencias Veterinarias

Universidad Austral de Chile

Valdivia, Chile

1
Marcell Gatica

Profesora asistente

Bioquímica y Biología Molecular

Universidad de Concepción

Concepción, Chile

1
Gustavo Moraga

Profesor Asistente

Departamento de Fisiología

Universidad De Concepción

Concepción, Chile

1
María Rauch

ACADEMICO

INSTITUTO DE BIOQUÍMICA Y MICROBIOLOGIA

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
MAFALDA MALDONADO

PROFESOR ASOCIADO

Fisopatología

UNIVERSIDAD DE CONCEPCION

Concepción, Chile

1
Carolina Muñoz

Colaboradora Docente, Investigadora

Fisiopatologçia

Universidad de Concepción

Concepción, Chile

1
Carlos Soliz

Coordinador del área de Fisiología Sede Concepción

Ciencias Biológicas

Universidad Andrés Bello

Talcahuano, Chile