Biochemestry of Vitamin C Molecular and Structural biology of transports Antioxidants, vitamin C, oxidative stress

TEACHING Experience.
I. University of Concepción 2014.

Undergraduate
212600 Thesis Project Formulation (Biochemestry)
240405 Research Unit Biology,
240502 Proyect title seminar (Biología)
242554 Proyect title seminar, (Ing Marine Biotechnology and Aquaculture)
250307 Workshop V: Science Soc and Tec.ecnología y Sociedad (Bioengineering)
250401 Pathophysiology Topic Molecular Cancer Gene Therapy (Bioengineering)
250503 Advanced Cell Biology Topics, (Bioengineering)
250508 Thesis Bioengineering,
251121 Basic Cell Biology (Nursing)
251501 Bioq III Research Unit and Seminar Bibliographical, Special Biochemistry, (Biochemestry)
253205 Pathophysiology (Biochemistry)
253205 Physiology (Biochemistry)
253217 Physiology and Pathophysiology I
254202 Pathophysiology (Nursing)
254203 Pathophysiology (Obstetrics)
254210 Pathophysiology Fonoaudiología (Fonoaudiología)
254301 Pathophysiology Biochemistry (Biochemistry)
254302 Pathophysiology (Dentistry)
254303 Pathophysiology (Chemistry and Pharmacy)
254309 Cell culture Applications (Bioengineering, Biochemistry, Biol)
254311 Biosafety in Laboratory (Biochemistry)
251121 Basic Cell Biology (Nursing)
251501 Bioq III Research Unit and Seminar Bibliographical, Special Biochemistry (Biochemistry)
256206 Introduction to Biomedical Sciences II (Medicine)
256218 Introduction to Biomedical Sciences IV (Medicine)

733352 Práctica Traducción Inglés-Español III, Traducción / Interpretación en Idiomas Extranjeros

Postgrade Teaching

PhD Program in Biological Science, Cellular and Molecular Biology
4235991 Thesis proyect Formulation
4235991 Professor Thesis Committe Thesis proyect
406894 Research Unit I
406834 Research Unit II
406895 Bibliographic Seminar I
406831 Bibliographic Seminar II
406834 Unidad de Investigación II

Master in Biochemistry and Bioinformatics.
4115005 Research unit I
4115006 Research unit II
4115007 Bibliographic Seminar I
4115008 Bibliographic Seminar II
4115012 Thesis project formulation
4115013 Candidate

Master in Physiology .
401425 Topics on cancer pathophysiology
401427 Cell biology and pathology
4111003 Research Unit II
4111006 Topics on cancer pathophysiology
4111007 Seminar II
4111991 Thesis project formulation

Master program in Human Physiology

4111011 Introduction to Molecular Pathology
4111993 Thesis Dissertation
4111991 Seminar I

Obstetrics, Specialization in Gynecology
500603 Specialization in Gynecology for Obstetricians

Obstetrics, Specialization in en Perinatology
500554 Specialization in Perinatology for Obstetricians

Direction of grade Thesis

Direction of undergrade Thesis
1. Student: Felipe Ignacio Paredes Díaz. Career: Biochemestry, University of Concepción. Thesis: Molecular analysis of regulation of the expression of Vitamin C transporter in human cells CaCo2. Finalized 2010.

2. Student: David Andrés Escobar Flores. Career: Biochemestry, University of Concepcion. Thesis: Development of a method bioinformatic for the identification of amino acids with functional - structural importance of membrane proteins. Finalized 2010.

3. Student: Maria de Los Angeles Sepúlveda Parada. Career: Biochemestry, University of Concepcion. Thesis: Analysis of the functional importance of residues aminoacídicos of the domain of exofacial union of the carrier of acid ascórbico SVCT1. Finalized 2012.

4. Student: Karen Susan Sweet Alvarado. Career: Biochemestry, University of Concepcion. Thesis: Identification and functional characterization of cysteine residues with the potential regulatory in the ascorbic acid transporter SVCT2. Finalized 2012.

5. Student: Ibeth Alejandra González Galdames. Career: Biología, University of Concepcion. Thesis Seminar: Modulation of the transcriptional activity of rat promoter of SVCT1 mediated by exogenous oxidative stress in rat hepatoma cells H4IIE. Finalized 2012.

6. Student: Carolina Muñoz Flores. Career: Bioengineering, University of Concepcion. Thesis: Molecular tools directed the metabolism of vitamin C in cancerous cells by modifying SVCT2 transporter expression. Finalized Marzo 2013.

7. Student: Natalia Cristhy Belmar Sazón. Career: Engineering in Marine Biotechnology and Aquaculture, Faculty of Natural Sciences and Oceanography, University of Concepcion. Seminar Title: Development of molecular tools for the analysis of the systems of vitamin C transport in fishs. In Execution.

Professor Evaluation Committee of Undergraduate Thesis
1. Student: Francisco Javier Roa Cartes. Career: Biochemestry, University of Concepcion. Thesis Regulating the expression of Vitamin C transporter in response to oxidative stress in breast cancer cells. Finalized 2011.

2. Student: Jorge Luis Vera Becerra. Career: Biochemestry, Universidad Austral de Chile. Thesis: characterization of residues involved in exofacial junction of substrates in the ascorbic acid transporter ascórbico SVCT2. Finalized 2011.

4. Student: Marisel Dominique Medina Nuñez. Career: Biólogo, University of Concepcion. Seminario de Título: TrxR1 Participation in the accumulation and recycling of vitamin C on prostate cancer. Finalized 2011.

5. Student: Esteban Francisco Caamaño Molina. Career: Licenciatura en Biología, University of Concepcion. Reserch Unit: Standardization of an in vitro model of hepatic oxidative stress induced by carbon tetrachloride.. Finalized 2012.

6. Student: Paulina Andrea Saavedra Sieyes. Career: Licenciatura en Biología, University of Concepcion. Thesis: Expression of corepresores SMRT and NCOR in prostate cancer. Finalized 2012.

7. Student: Cristina Angélica Padilla Ruiz. Career: Biochemestry, University of Concepcion. Thesis: Cloning, expression and functional characterization of SVCT3, a new member of the family of ascorbic acid transporters. Finalized 2012.

8. Student: Esteban Francisco Caamaño Molina. Career: Biología, University of Concepcion. Thesis Seminar Biologist: Protective effect of glutathione in an in vitro model of hepatic oxidative stress induced by carbon tetrachloride. Finalized March 2013.

Direction of postgrade Thesis

Direction of Master Thesis
1. Student: Carolina Muñoz Flores. Career: Bioengineering, University of Concepcion. Thesis: Molecular alteration and pharmacology of metabolism of vitamin C in human cells. Thesis.

2. Student: Karen Susan Sweet Alvarado. Career: Biochemestry, University of Concepcion. Thesis: Saturating mutagenesis of the pathway translocation substrate in transporter ascorbic acid SVCT2.. Thesis.

Professor Committee Magister Thesis Evaluation
1. Student: Perla Kathanina Salgado Mejias. Programa: Master of Clinical Biochemistry and Immunology, University of Concepcion. Thesis Title: Protective effect of cellular GSH in the defense to oxidative stress by exposure to snuff smoke in human endofaciales cells. Finalized 2010.

2. Student: Claudia Solange Jaña Martínez. Programa: Master in Biochemistry and Bioinformatics, University of Concepcion. Título Thesis: Cloning, expression and characterization of dehydroascorbate reductase in human tumor cells. Finalized 2011.

3. Student: Pablo Antonio Binder Saldaña. Programa: Master in Biochemistry and Bioinformatics, University of Concepcion. Título Thesis: Functional characterization of the promoter region of reductase dehydroascorbic acid 1 glutarredoxina. Finalized 2011.

4. Student: Eduardo Andrés Peña Alarcón. Programa: Master in Science mention Physiology, University of Concepcion. Título Thesis: Differential expression of Vitamin C transporters in breast cancer cells negative for the estrogen receptor. Finalized 2011.

5. Student: Marilyn Denisse Vergara Valenzuela. Programa: Master of Clinical Biochemistry and Immunology, University of Concepcion. Thesis Title: Molecular analysis of the expression of Vitamin C transporters in colon cancer. Finalized 2012

Direction of Phd Thesis

1. Alexis Salas. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Development of robust methods for predicting 3D structures of membrane proteins and their application to the ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. Finalized 2011.

2. Student: María Elizabeth Escobar Sagredo. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mutational analysis of a domain-binding EF-hand calcium vitamin C transporter SVCT1. PhD Scholarship CONICYT. Finalized 2012.

6. Student: Henny Marianne Haensgen Sáez. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Structural determinants translocation pathway substrate in ascorbic acid transporter SVCT1. PhD Scholarship CONICYT. In Execution.

7. Student: Carlos Francisco Aylwin Ramírez. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Construction of a transporter without cysteine (Cys-less) and structural and functional characterization of the migration path substrates ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. In Execution.

8. Student: Paula Angélica Guzmán González. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Transcriptional regulation of vitamin C transporters SVCT1 and SVCT2 during differentiation and response to oxidative stress in human intestinal cells CaCo-2. PhD Scholarship CONICYT. In Execution.

9. Student: Alejandra Andrea Muñoz Vega. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Characterization of the promoter region of the gene for vitamin C transporter rat SVCT2. PhD Scholarship CONICYT. In Execution.

10. Student: Marcell Alejandra Gatica Miranda. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Quaternary structure of transporter vitamin C SVCT2. PhD Scholarship CONICYT. In Execution.

11. Student: Mafalda Eliana Maldonado Villagrán. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Early regulation and molecular determinants of vitamin C metabolism post-partial hepatectomy in rats. In Execution.

12. Student: Pamela Mendoza. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mutational analysis saturating pathway translocation substrate ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. In Execution.

13. Student: Mauricio Alejandro Gonzalez Oyarzún. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mechanistic aspects and structural determinants of transport cycle ascorbic acid transporters plasma membrane and intracellular. PhD Scholarship MECESUP. In Execution.

14. Student: Francisco Javier Roa Cartes: Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Role of transporter vitamin C SVCT2 in mitochondria in human cells. PhD Scholarship CONICYT. In Execution.

EXTENSION

Transfer to community impact.

2010 Biomedicine Workshop 2.0. Designing a Science Company linking strategy, TABLE III: Workshop of Cluster BíoBío Health, Genetic Engineering Workshop for Leaders of Bio-Bio Region. The workshop was attended by representatives of companies, government agencies Chile earmarked to financing R & D and regional print media. Antioxidants Laboratory, School of Biological Sciences, University of Concepción, January 27, 2010.

2011 Symposium “New Pharmacological Therapies in Cancer. Molecular basis of antitumor therapy”. XXXIII Annual Congress of Chile Pharmacology Society, Olmué, Chile, 16-19 November 2011. Journal of Pharmacology, Chile 4(1): 29. Organizer.

Popular articles in magazines, newspapers and other media.

2010 Scientists for a Day: Genetic Engineering Workshop: Southern Journal, Sunday January 31, 2010.

2010 Interview Program Conceptions of TVU, "Research in Cancer" broadcast on Thursday 9th December at 20:0 pm and rebroadcast on Sunday 12th at 20:40 hours.

2012 UdeC Scientists create transgenic mice with novel technique.
Panorama UdeC, 26 de July.