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Daniel Gonzalo Paredes Sabja

Associate Professor

Universidad Andrés Bello

Santiago, Chile

Líneas de Investigación


The main research questions that drive our research are:1) How pathogenic C. difficile interacts with the intestinal mucosa, innate and adaptive immune system. 2) How the intestinal microbiota modulates intestinal homeostasis

Educación

  •  Ingeniero en Alimentos, Universidad Austral de Chile. Chile, 2003
  •  Molecular Microbiology, Oregon State University. Estados Unidos, 2009

Experiencia Académica

  •   Profesor Asistente Full Time

    Universidad Andrés Bello

    Facultad de Ciencias Biológicas

    Santiago, Chile

    2011 - 2014

  •   Profesor Asociado Full Time

    Universidad Andrés Bello

    Facultad de Ciencias Biológicas

    Santiago, Chile

    2014 - At present

Experiencia Profesional

  •   Jefe de Procesos de Ahumado Full Time

    Ventisqueros

    Hornopirén, Chile

    2002 - 2004

  •   Assistant Professor Full Time

    Universidad Andres Bello

    Santiago, Chile

    2011 - 2014

  •   Associate Professor Full Time

    Universidad Andres Bello

    Santiago, Chile

    2014 - At present

Formación de Capital Humano


Doctoral students trained:
Christian Brito Ph.D. in Molecular Biosciences. 2012-to date
Marjorie Pizarro Ph.D. in Biotechnology. 2013-to date
Paulina Calderón Ph.D. in Molecular Biosciences. 2014-to date
Angela Plaza Ph.D. in Molecular Biosciences. 2015-to date
Pablo Castro Ph.D. in Biotechnology. 2016-to date
Rodrigo Reyes Ph.D. in Molecular Biosciences. 2017-to date

Master students trained:
Enzo Guerrero Master in Biotechnology. 2016-to date.
Francisca Cid Master in Biotechnology. 2016-to date.
Rodrigo Reyes Master in Biochemistry. 2016-to date.
Paola Mora Master in Biotechnology. 2015-to date.
Macarena Otto Master in Biotechnology. 2015-to date.
Juan Muñoz Master in Biotechnology. 2015-to date.
Valentina Camilli Master in Biotechnology. 2015-to date.
Constanza Galilea Master in Biotechnology. 2015-2017.
Rodrigo Reyes Master in Biochemistry. 2015-2016.
Juan Pablo Peñaloza Master in Molecular Biosciences. 2015-2017
Jaime Pizarro. Master in Biochemistry. 2014-2016.
Fernando Díaz. Master in Biotechnology. 2014-2016
Pablo Castro. Master in Biotechnology. 2014-2016
Catalina Rosales Master in Biotechnology. 2014-2015
Rodrigo Pérez Master in Molecular Biosciences. 2013-2015
Glenda Cofré. Master in Biochemistry. 2012-2014.
Mauro Milano. Master in Biochemistry. 2012-2014.
Camila Miranda. Master in Biochemistry. 2012-2014.

Advisor of Research Units of undergraduate students:
Guillermo Carrasco: 2011
Glenda Cofré-Araneda: 2011-2012
Valeria Olguin: 2011-2012
Camila Miranda-Cárdenas: 2012
Angela Plaza-Garrido: 2012
Jaime Pizarro: 2012-2014
Catalina Díaz: 2012-2013.
Flavia Dorogeschi: 2013-2013

Undergraduate students:
Jonathan Barra. Veterinary Medicine. 2012.


Premios y Distinciones

  •   Outstanding Doctoral Student Savery Award

    OREGON STATE UNIVERSITY

    Estados Unidos, 2009

    Awarded to the most outstanding doctoral student (research productivity and GPA) in the College of Agricultural Sciences at the Oregon State University

  •   Oregon Lottery Scholarship

    OREGON STATE UNIVERSITY

    Chile, 2009

    Awarded to graduate students at OSU with academic merit in research productivity and course work

  •   Oregon Lottery Scholarship

    OREGON STATE UNIVERSITY

    Chile, 2008

    Awarded to graduate students at OSU with academic merit in research productivity and course work

  •   Graduate Student Research Award

    OREGON STATE UNIVERSITY

    Chile, 2008

    Awarded to the graduate student with the highest research productivity of the College of Veterinary Medicine at OSU.

  •   Chilean Presidential Fellowship.

    Ministerio de Planificación

    Chile, 2004

    Sponsors the formation of outstanding scientists covering graduate tuition and a partial stipend to pursue a Ph.D. degree.

  •   Academic Efficiency Award.

    UNIVERSIDAD AUSTRAL DE CHILE

    Chile, 2003

    Sponsored by “Levaduras Collico, Chile” Consisting in the highest coefficient of GPA divided in the number of semesters remained in the Universidad Austral de Chile (UACH), of students from the same promotion.

  •   Premio al Científico Joven

    SOCIEDAD DE BIOLOGIA DE CHILE

    Chile, 2015

    Premio al científico joven más destacado.

  •   Premio al Científico Joven más destacado

    UNIVERSIDAD ANDRES BELLO

    Chile, 2016

    Premio al profesor joven más destacado en cuanto a publicaciones e investigación.


 

Article (86)

Survival of Clostridium difficile spores at low water activity
Updates on the biology of Clostridium difficile spore.
A feed-forward loop between SroC and MgrR small RNAs modulates the expression of eptB and the susceptibility to polymyxin B in Salmonella Thyphimurium
Acyldepsipeptide antibiotics as a potential therapeutic agent against Clostridium difficile recurrent infections
Characterization of germinants and their receptors for spores of non-food-borne Clostridium perfringens strain F4969
Clostridium perfringens sporulation and sporulation-associated toxin production.
Genome Sequence of Clostridium paraputrificum 373-A1 Isolated in Chile from a Patient Infected with Clostridium difficile
Lose to win: marT pseudogenization in Salmonella enterica serovar Typhi contributed to the surV-dependent survival to H2O2, and inside human macrophage-like cells
Predominance of Clostridium difficile ribotypes 012, 027 and 046 in a university hospital in Chile, 2012
Salmonella Typhimurium exhibits fluoroquinolone resistance mediated by the accumulation of the antioxidant molecule H2S in a CysK-dependent manner
The integrity of the exosporium layer affects tropism of Clostridium difficile epidemic R20291 spores towards components of the intestinal mucosa
The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion
Ultrastructural variability of the exosporium layer of Clostridium difficile spores from sporulating culture and biofilms.
Ultratuctural variability of the exosporium layer of Clostridium difficile spores.
Analysis of Vibrio vulnificus Infection Risk When Consuming Depurated Raw Oysters
CysB-dependent upregulation of the Salmonella Typhimurium cysJIH operon in response to antimicrobial compounds that induce oxidative stress
Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores
Motility modulation by the small non-coding RNA SroC in Salmonella Typhimurium
Outcome of relapsing Clostridium difficile infections do not correlate with virulence-, spore- and vegetative cell-associated phenotypes
Participation of S. Typhimurium cysJIH operon in the H2S-mediated ciprofloxacin resistance in presence of sulfate as sulfur source.
Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores
Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi
The inhibitory effects of sorbate and benzoate against Clostridium perfringens type A isolates
Updates on the sporulation process in Clostridium species
Characterization of the collagen-like exosporium protein, BclA1, of Clostridium difficile spores
Clostridium difficile spores and its relevance in the persistence and transmission of the infection
Clostridium difficile spores: a major threat to the hospital environment
New amino acid germinants for spores of the enterotoxigenic Clostridium perfringens type A isolates
Performance of prognostic index in severe Clostridium difficile-associated infection. Retrospective analysis in a university hospital
Recent advances in germination of Clostridium spores
Atypical presentation of pseudomembranous colitis localized in adenomatous polyps
High hydrostatic pressure induced inactivation of bacterial spores
Inactivation strategy for Clostridium perfringens spores adhered to food contact surfaces.
Prospective comparison of a commercial multiplex real-time PCR and an enzyme immunoassay with toxigenic culture in the diagnosis of Clostridium difficile-associated infections
Prospective comparison of a commercial multiplex real-time polymerase chain reaction and an enzyme immunoassay with toxigenic culture in the diagnosis of Clostridium difficile-associated infections
Proteases and sonication specifically remove the exosporium layer of spores of Clostridium difficile strain
Proteases and sonication specifically remove the exosporium layer of spores of Clostridium difficile strain 630
The Clostridium difficile Exosporium Cysteine (CdeC)-Rich Protein Is Required for Exosporium Morphogenesis and Coat Assembly
The Clostridium perfringens Germinant Receptor Protein GerKC Is Located in the Spore Inner Membrane and Is Crucial for Spore Germination
Unique Regulatory Mechanism of Sporulation and Enterotoxin Production in Clostridium perfringens
Adherence of Clostridium difficile spores to Caco-2 cells in culture
Clostridium difficile associated infections: an updated view
Clostridium difficile Spore-Macrophage Interactions: Spore Survival
Effects of wet heat treatment on the germination of individual spores of Clostridium perfringens
Infecciones asociadas a Clostridium difficile: una visión actualizada.
Inhibitory Effects of Nisin Against Clostridium perfringens Food Poisoning and Nonfood-Borne Isolates
Interactions between Clostridium perfringens spores and Raw 264.7 macrophages
The inhibitory effect of nisin on enterotoxigenic Clostridium perfringens Type A isolates
Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity
Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity.
Clostridium perfringens tpeL is expressed during sporulation
Clostridium perfringens tpeL is expressed during sporulation.
Germination of spores of Bacillales and Clostridiales: mechanisms and proteins involved. T
Germination response of spores of the pathogenic bacterium Clostridium perfringens and Clostridium difficile to cultured human epithelial cells.
Host serum factor triggers germination of Clostridium perfringens spores lacking the cortex hydrolysis machinery
Host serum factor triggers germination of Clostridium perfringens spores lacking the cortex lytic hydrolysis machinery.
Effect of a cortex-lytic enzyme, SleC, from a non-food borne Clostridium perfringens on the germination properties of SleC-lacking spores of food poisoning isolate.
Modeling of the Germination of Spores from Clostridium perfringens food poisoning isolates.
Further characterization of Clostridium perfringens small acid soluble protein-4 (Ssp4) properties and expression.
Clostridium perfringens sporulation, and its relevance to pathogenesis
Clostridium perfringens sporulation, and its relevance to pathogenesis.
Further characterization of Clostridium perfringens small acid soluble protein-4 (Ssp4) properties and expression
GerO, a putative Na+/K+-H+ antiporter, is essential for normal germination of Clostridium perfringens spores
GerO, a putative Na+/K+-H+ antiporter, is essential for normal germination of Clostridium perfringens spores.
Inorganic phosphate and sodium ion as co-germinants for spores of Clostridium perfringens type A food poisoning isolates
Inorganic phosphate and sodium ion as co-germinants for spores of Clostridium perfringens type A food poisoning isolates.
SleC is essential for cortex peptidoglycan hydrolysis during germination of spores of the pathogenic bacterium Clostridium perfringens.
Strategy to inactivate Clostridium perfringens spores in meats
Strategy to inactivate Clostridium perfringens spores in meats.
The protease CspB is essential for initiation of cortex hydrolysis and DPA release during spore germination of Clostridium perfringens type A food poisoning isolates
The protease CspB is essential for initiation of cortex hydrolysis and DPA release during spore germination of Clostridium perfringens type A food poisoning isolates.
Characterization of Clostridium perfringens spores that lack SpoVA proteins and dipicolinic acid.
Clostridium perfringens spore germination: characterization of germinants and their receptors.
Germination of Spores of Clostridium difficile Isolates from a Hospital Outbreak of Clostridium difficile-associated disease (CDAD).
Inhibitory effects of polyphosphates on Clostridium perfringens growth, sporulation and spore outgrowth.
Role of Small, Acid-soluble Spore Proteins in the Resistance of Clostridium perfringens Spores to Chemicals.
Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals and UV radiation.
Clostridium perfringens: Sporulation, Spore Resistance and Germination.
Combined effects of Hydrostatic Pressure, Temperature and pH on the Inactivation of Spores of Clostridium perfringens type A and Clostridium sporogenes in buffer solutions.
Microbial growth in refrigerated meat products.
Diagnostic accuracy of a multiplex real-time PCR product to predict Clostridium difficile ribotype 027
Germination of spores of Bacillales and Clostridiales species: mechanisms and proteins involved.
Hurdle Approach to Increase the Microbial Inactivation by High Pressur Processing: Effects of Essential Oils.
Role of GerKB in germination and outgrowth of Clostridium perfringens spores.
SleC is essential for cortex peptidoglycan hydrolysis during germination of spores of the pathogenic bacterium Clostridium perfringens
Survival of Clostridium difficile Spores at Low Temperatures

Letter (2)

Diagnostic accuracy of a multiplex real-time PCR to predict Clostridium difficile ribotype 027
Epidemic Clostridium difficile Ribotype 027 in Chile

Review (5)

Clostridium difficile recurrent infection: possible implication of TA systems
High hydrostatic pressure-induced inactivation of bacterial spores
Clostridium difficile spore biology: sporulation, germination, and spore structural proteins
Hurdle Approach to Increase the Microbial Inactivation by High Pressure Processing: Effect of Essential Oils
Molecular basis of early stages of Clostridium difficile infection: germination and colonization
96
Daniel Paredes

Associate Professor

Department of Biological Sciences

Universidad Andrés Bello

Santiago, Chile

9
Manuel Alvarez

Gastroenterologist and Chief of Institute of Medicine

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

7
IVAN CALDERON

Associate Professor / Principal Investigator/ Master in Biotechnology & Life Sciences Director

Biological Sciences

UNIVERSIDAD ANDRÉS BELLO

SANTIAGO, Chile

7
Juan Fuentes

Académico

Ciencias Biológicas

Universidad Andrés Bello

Santiago, Chile

5
Fernando Gil

Associate Professor

Biological Sciences

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

5
Valeria Olguín

Lab Manager

Universidad del Desarrollo

Santiago, Chile

5
Marjorie Pizarro

Asistente de Investigacion

Ciencias Biológicas

Universidad Andrés Bello

Santiago, Chile

4
Angela Plaza

Postodoctorado

Ingeniería Civile en Obras Civil

Universidad de Santiago de Chile

Estación Central, Chile

4
Paula Rodas

Scientific affairs

Agencia Chilena para la Inocuidad Alimentaria (ACHIPIA)

Santiago, Chile

2
Guido Mora

Académico/Investigador

Facultad Ciencias de la Salud

Universidad SEK

Santiago, Chile

2
Christian Brito

Postdoc

Universidad Andrés Bello

Santiago, Chile

2
Ítalo Urrutia

Profesor Asistente

Universidad Autónoma de Chile

Santiago, Chile

2
Cristian Hernández

Assistant Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

1
José Zoroquiain

PONTIFICIA UNIVERSIDAD CATÓLICA

SANTIAGO, Chile

1
Eduardo Castro

Professor

Departamento de Microbiología

Universidad de Talca

Talca, Chile

1
Paulina Calderón

Investigador Principal

Biome Therapeutics

Santiago, Chile

1
Enzo Guerrero

PhD Student

Universidad Andrés Bello

Santiago, Chile

1
Alejandro Hidalgo

Associate Professor

Escuela de Química y Farmacia

Universidad Andres Bello

Santiago, Chile

1
Sebastián Naour

Gestor Operacional de Transferencia Tecnológica

Vicerrectoría de Transferencia Tecnológica y Extensión

Universidad Tecnológica Metropolitana

Santiago, Chile

1
Claudia Saavedra

Full Professor

Departmento de Ciencias de la Vida

UNIVERSIDAD ANDRÉS BELLO

Santiago, Chile

1
Ana Guzman

MÉDICO JEFE LABORATORIO

LABORATORIO CLÍNICO

PONTIFICIA UNIVERSIDAD CATÓLICA

SANTAIGO, Chile

1
Sandro Valenzuela

Bioinformático

Bioinformática

Multiplex SpA

Santiago, Chile

1
Susan Bueno

Profesor Asociado

Genética Molecular y Microbiología

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Patricio Ibañez

Asistant professor

Gastroenterology

PONTIFICIA UNIVERSIDAD CATOLICA

Santiago, Chile