Man

Maria-Isabel Yuseff Sepulveda

Associate Professor

Pontificia Universidad Católica de Chile

Santiago, Chile

Líneas de Investigación


Antigen processing and presentation by B Lymphocytes Immune synapse Cell polarity and membrane trafficking Cytoskeleton Dynamics Autoimmunity

Educación

  •  BSc, Universidad de Concepción. Chile, 1998
  •  PhD, Universidad de Chile. Chile, 2004

Experiencia Académica

  •   Associate Professor Full Time

    P. Universidad Católica de Chile

    Sciences

    Santiago, Chile

    2013 - At present

Experiencia Profesional

  •   Post Doctoral Fellow

    Curie Institute

    Paris, Francia

    2006 - 2013

  •   Post Doctoral Fellow

    P. Universidad Católica de Chile

    Santiago, Chile

    2004 - 2006

Formación de Capital Humano


1-Training of Students

• Thesis co-director conducing to the degree of PhD in Cellular and Molecular Biology and Neurosciences. Juan José Sáez, PhD program of Cellular and Molecular Biology and Neurosciences, Faculty of Sciences, Universidad de Chile. Thesis project entitled: “Exo70 regula la eficiente fusión de lisosomas en la sinapsis inmune”. 2014-2018.

• Thesis director conducing to the degree PhD in Cellular and Molecular Biology. Jorge Andres Ibañez Vega, PhD program of Cellular and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile. Thesis entitled: "Rol del proteosoma en la formacion y funcion de la sinapsis inmune en linfocitos B”. 2016-2019.

• Thesis director conducing to the degree PhD in Cellular and Molecular Biology. Danitza Natalia Fuentes Peña, PhD program of Cellular and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile. Thesis entitled: "Rol del receptor de KDEL en la formación y función de la Sinapsis inmune en Linfocitos B”. 2017-Present.

• Thesis director conducing to the degree PhD in Medical Sciences. Jonathan Alexander Lagos Orellana, PhD program of Medical Sciences, Faculty of Medicine, Pontificia Universidad Católica de Chile. Thesis entitled: "Regulación de la autofagia por señales inflamatorias y su impacto en la presentación de antígenos en linfocitos B”. 2018-Present.

• Thesis director conducing to the degree of Biochemist. Fernanda Estefanía Cabrera Reyes. Thesis entitled: "Caracterización de la proteína SNX5 durante la activación de linfocitos B". 2018 - Present.
• Thesis director conducing to the degree of Biochemist. Martina Alamo Rollandi. Thesis entitled: "Título “SNAP23 regula la extracción y presentación de antígeno promoviendo la secreción de lisosomas hacia la sinapsis inmune". 2016 - 2018.

• Thesis director conducing to the degree of Biologist. Christopher Rivas Needham. Thesis entitled: "Dinámica Nuclear en Linfocitos B". 2019 - Present.
• Thesis director conducing to the degree of Biologist. Felipe del Valle Batalla. Thesis entitled: "Rol del proteosoma y su relación con el centrosoma en la sinapsis immunológica de Linfocitos B". 2016.
• Thesis director conducing to the degree of Biologist. Juan Pablo Bozo Olea. Thesis entitled: "Caracterización del papel del complejo exocisto en la formación de la sinapsis inmunológica de linfocitos B". 2015.

2007-2013: Trained and supervised the research of two graduate student from Université Paris VII Diderot-Paris during their masters’ internship (M2) and PhD thesis .
1997: Teachers assistant in the course of Microbiology in the University of Lleida (INTERCAMPUS AL.E.97) as part of an exchange program (1997).
1995: Teachers assistant in courses of Organic Chemistry (1995) Department of Chemistry. University of Concepcion, Concepcion, Chile.

2-Courses:
-Graduate Course in Cell biology: Cytoskeleton and Cell Polarity. June 17-20th, 2014
-Summer School Cell biology Course: Processing and Presentation of Antigens by B cells. Jan 20th, 2014

3-Symposiums:
Symposium on Immune Regulation. “From Basic Science to Novel Immuno therapeutic Strategies”. Nov 7th, 2013. Facultad de Medicina Universidad de Chile. Invited Speaker.


Premios y Distinciones

  •   Best Oral Presentation in Young Researchers in Life Sciences Meeting Paris, France.

    INSTITUT CURIE DE PARIS

    Francia, 2008

    Awarded first prize for best oral presentation in Young Researchers in Life Sciences Meeting Paris, France.

  •   Embo Global Investigator

    European Union

    Chile, 2019

    Funding by Embo is given to early-career scientists based on their scientific merit, where researchers must demonstrate that they have successfully established themselves as independent researchers by presenting their projects and having published articles as a last/corresponding author. The enables scientists to establish collaborations with scientists from the Embo community and enhance training opportunities for myself and students from my group. Type


 

Article (27)

Clinical and pulmonary function analysis in long-COVID revealed that long-term pulmonary dysfunction is associated with vascular inflammation pathways and metabolic syndrome
Medium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization
Autophagy Induced by Toll-like Receptor Ligands Regulates Antigen Extraction and Presentation by B Cells
B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction
Alterations in Lysosome Homeostasis in Lipid-Related Disorders: Impact on Metabolic Tissues and Immune Cells
Alterations in Lysosome Homeostasis in Lipid-Related Disorders: Impact on Metabolic Tissues and Immune Cells
Dopaminergic stimulation leads B-cell infiltration into the central nervous system upon autoimmunity
Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse
KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover
Proteasome Dependent Actin Remodeling Facilitates Antigen Extraction at the Immune Synapse of B Cells
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells
Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse
Tuning B cell responses to antigens by cell polarity and membrane trafficking
Vamp-7-dependent secretion at the immune synapse regulates antigen extraction and presentation in B-lymphocytes
Actin nucleation at the centrosome controls lymphocyte polarity
Polarity protein Par3 controls B-cell receptor dynamics and antigen extraction at the immune synapse
How B cells capture, process and present antigens: a crucial role for cell polarity
Polarized Secretion of Lysosomes at the B Cell Synapse Couples Antigen Extraction to Processing and Presentation
Dynamics of membrane trafficking downstream of B and T cell receptor engagement: impact on immune synapses
Regulation of Dendritic Cell Migration by CD74, the MHC Class II-Associated Invariant Chain
A cytoplasmic PPPSP motif determines Megalin's phosphorylation and regulates receptor's recycling and surface expression
Antibody to AP1B adaptor blocks biosynthetic and recycling routes of basolateral proteins at recycling endosomes
Syk-dependent actin dynamics regulate endocytic trafficking and processing of antigens internalized through the B-cell receptor
Differential distribution of low-density lipoprotein-receptor-related protein (LRP) and megalin in polarized epithelial cells is determined by their cytoplasmic domains
Identification and characterization of GONST1, a Golgi-localized GDP-mannose transporter in Arabidopsis
Cooper (III) Dithiocarbamate Complexes From Thiuram disulfides and Cooper (I) Halides

BookSection (1)

Studying MHC II Presentation of Immobilzed Antigen by B Lymphocytes

Proyecto (2)

Shaping the Immune Synapse by Centrosome-Associated Proteins=> Impact on B Cell Responses
REGULATION OF IMMUNE SYNAPSES THROUGH CELL POLARITY=> IMPACT ON B CELL RESPONSES

Review (2)

Lysosome motility and distribution: Relevance in health and disease
B cells use conserved polarity cues to regulate their antigen processing and presentation functions
34
Maria-Isabel Yuseff

Associate Professor

Cellular and Molecular Biology

Pontificia Universidad Católica de Chile

Santiago, Chile

4
Jheimmy Diaz

Investigador Adjunto

Biología Celular y Molecular

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Andrea Soza

Profesor Asociado

Medicina y Ciencia

Universidad San Sebastián

Santiago, Chile

3
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Fanny Guzman

Investigador Senior

Núcleo de Biotecnología Curauma

Pontificia Universidad Católica de Valparaiso

Valparaiso, Chile

2
Maria Paz Marzolo

Profesor Titular

Departamento de Biología Celular y Molecular

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE, FAC. CIENCIAS BIOLOGICAS, DEPTO. BIOLOGIA

Santiago, Chile

2
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Claudia Oyanadel

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Fabián Segovia

Associate professor

Department of Cell Biology

Universidad de Concepción

Concepción, Chile

1
Alejandra Alvarez

Full professor

Cell and Molecular Biology

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE, FACULTAD DE CIENCIAS BIOLÓGICAS

Santiago, Chile

1
Steffen Härtel

Full Professor

Instituto de Ciencias Biomédicas

University of Chile

Santiago, Chile

1
Juan Oyarzún

Investigador postdoctoral

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Viviana Cavieres

Postdoctoral Researcher

Medicina y Ciencias

Universidad San Sebastián

Santiago , Chile

1
Jorge Cancino

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

1
Diego Tapia

Asistente de Investigación

Universidad San Sebastián

Santiago, Chile

1
Jorge Jara

Investigador Posdoctoral

ICBM, Facultad de Medicina

Universidad de Chile

Santiago, Chile

1
Gonzalo Labarca

Assistant Profesor in Medicine

Medicina interna

Pontificia Universidad Católica de Chile

Santiago, Chile

1
PATRICIA BURGOS

Associate Professor Programa de Doctorado en Biología Celular y Biomedicina CEBICEM

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

1
GONZALO MARDONES

Associate Professor

Medicine

Universidad San Sebastián

Valdivia, Chile

1
Gloria Arriagada

Profesor Asociado-Investigador Principal

Instituto de Ciencias Biomédicas

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

1
Michael Handford

Full Professor

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE BIOLOGÍA

Santiago, Chile

1
Mario Henríquez

Investigador

CIBERES

CIBER

Lleida / Lérida, España

1
Fernando Ezquer

Full Professor

Center for Regenerative Medicine

Universidad del Desarrollo

Santiago, Chile

1
alejandro amoroso

Profesor Asistente

Deoartamento de Ciencias Biológicas y Químicas

Universidad San Sebastián

santiago, Chile

1
Mabel Vidal

Directora Magíster en Data Science

Universidad San Sebastián

Concepcion, Chile

1
Luis Quinones

PROFESOR TITULAR

ONCOLOGIA BASICO-CLINICA

UNIVERSIDAD DE CHILE

Santiago, Chile

1
PATRICIO VARGAS

Profesor Titular

FISICA

USM

Valparaiso, Chile

1
Luis González

Full Professor

School of Medicine

Universidad San Sebastián

Santiago, Chile

1
sergio sanhueza

Investigador

Biquímica Clínica e Inmunología

Universidad de Concepción

Concepcion, Chile

1
Ariel Orellana

Profesor Titular

Centro de Biotecnologia Vegetal

UNIVERSIDAD ANDRES BELLO

Santiago, Chile