Man

Eduardo Karahanian Vartevanian

Académico Investigador

Universidad Autónoma de Chile

Santiago, Chile

Líneas de Investigación


Alcoholism; Gene Therapy; Pharmacology; Alcoholism, animal models and interventions in humans; Mechanism of alcohol actions; Gene delivery and phage display libraries; Farmacologia (Neurofarmacologia); Neuroquimica; Neurociencias; Gene Therapy of Alcoholism; Mechanisms of Alcohol Addiction; Vectors for Gene Therapy; Alcohol intake; Drug Dependence

Educación

  •  Biología, UNIVERSIDAD DE CHILE. Chile, 2000
  •  Biología, Universidad de Buenos Aires. Argentina, 1994

Experiencia Académica

  •   Académico Investigador/Prodesor Asociado Full Time

    UNIVERSIDAD AUTONOMA DE CHILE

    Facultad de Ciencias de la Salud

    Santiago, Chile

    2013 - A la fecha

  •   Académico Investigador/Prodesor Asociado Full Time

    UNIVERSIDAD DIEGO PORTALES

    Facultad de Medicina

    Santiago, Chile

    2002 - 2013

  •   Instructor Part Time

    UNIVERSIDAD DE CHILE

    Facultad de Ciencias Químicas y Farmacéuticas

    Santiago, Chile

    2000 - 2002

  •   Investigador Postdoctoral Full Time

    UNIVERSIDAD DE CHILE

    Facultad de Ciencias Químicas y Farmacéuticas

    Santiago, Chile

    1999 - 2001

Formación de Capital Humano


Undergraduate thesis direction:
- Development of a multiplex PCR to detect different genes of metallo beta-lactamase of Pseudomonas aeruginosa. Alexis Luengo Yañez. Universidad Tecnológica Metropolitana, Facultad de Ciencias naturales matemáticas y del medio ambiente. 2014.
- Design and evaluation of a new system for gene silencing therapy for alcoholism. Dagoberto Sepúlveda Araneda. Facultad de Ciencias Básicas y Matemáticas, Pontificia Universidad Católica de Valparaíso. 2008.

PhD thesis direction:
- Evaluation of fenofibrate as a new drug for alcoholism treatment. Daniel Muñoz Uribe. Universidad Autónoma de Chile. 2020.
- Relationship between the melanocortin system and alcohol consumption in adolescence. Osvaldo Flores Bastías. Universidad Autónoma de Chile. Writing in preparation.

PhD committee:
- 2020, PhD thesis. Francisca Villavicencio, PhD program in Biomedical Sciences, thesis title:"LA ACTIVACIÓN DE LA VÍA ANTIOXIDANTE NRF-2 PREVIENE LA DISFUNCIÓN MITOCONDRIAL Y LA FALLA COGNITIVA INDUCIDA POR LA PRESENCIA DE FORMAS PATOLÓGICAS DE LA PROTEÍNA TAU EN LA ENFERMEDAD DE ALZHEIMER"
- 2017, PhD thesis, Hugo Díaz, PhD program in Biomedical Sciences, thesis title: “EFECTO DE ÁCIDO TAUROURSODEOXICÓLICO SOBRE LOS DESÓRDENES CARDIORRESPIRATORIOS EN INSUFICIENCIA CARDIACA” Universidad Autónoma, Santiago, Chile.
- 2016, PhD thesis, Alequis Pavón, PhD program in Biomedical Sciences, thesis title: “EFECTO PROAPOPTÓTICO Y ANTIMETASTÁSICO EN LÍNEAS TUMORALES HUMANAS COLORRECTALES DE UNA PROTEÍNA SECRETADA POR LA BACTERIA RIZOSFÉRICA ANTÁRTICA BACILLUS sp. K2I17”, Universidad Autónoma, Santiago, Chile.
- 2016, PhD thesis, Claudia Jara, PhD program in Biomedical Sciences, thesis title: "EFECTOS DE LA PROTEINA TAU SOBRE LA DISFUNCION MITOCONDRIAL Y EL DETERIORO COGNITIVO EN EL ENVEJECIMIENTO." Universidad Autónoma, Santiago, Chile.

PhD Courses
- "Advanced Molecular Biology" PhD program in Biomedical Sciences, Universidad Autónoma de Chile. 2014-current.
- "Molecular Biology for Biomedicine" PhD program in Biomedical Sciences, Universidad de Chile. 2015-current.
- "Advanced Molecular Pharmacology" PhD program in in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile. 1999-2014.
- "Molecular Biology and Advanced Genomics" Graduate Course # 555. Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile. 1999-present date.
- "Advanced Course in Human Genetics" PhD program in Biomedical Sciences, Universidad de Chile. 2000-2005


Difusión y Transferencia


International Meetings since 2010:

"Role of ethanol of metabolites in alcohol abuse and dependence” E. Karahanian. IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA). Córdoba, Argentina. 7-9 de noviembre, 2019.

“Adolescent binge alcohol consumption affects neuronal communication through the impairment of mitochondrial function in the adulthood” R. A. Quintanilla, C. Tapia-Rojas, W. Cerpa, J. A. Orellana, E. Karahanian, C. Osorio-Fuentealba. 41st Annual Research Society on Alcoholism (RSA) Scientific Meeting. San Diego, CA, USA. 16-20 de junio de 2018.

“Role of ethanol metabolites in alcohol abuse and dependence”. Rivera-Meza, M; Berríos-Cárcamo, P; Quintanilla, ME; Karahanian, E; Herrera-Marschitz, M; Israel, Y. LASBRA International Satellite: Neurobiological basis of alcoholism: from molecules to behavior. Puerto Varas, Chile. 20-22 de noviembre de 2017.

“Alcohol intake: from initiation to binge-drinking. mechanisms and translational opportunities.” Yedy Israel, María Elena Quintanilla, Paola Morales, Marcelo Ezquer, Eduardo Karahanian, Mario Herrera-Marschitz, Fernando Ezquer. LASBRA International Satellite: Neurobiological basis of alcoholism: from molecules to behavior. Puerto Varas, Chile. 20-22 de noviembre de 2017.

“Fenofibrate-induced reduction of voluntary ethanol intake in UChB rats: evidence of peripheral and central effects” Rivera-Meza M, Jerez E, Muñoz D, Quintanilla ME, Karahanian E, Israel Y. 16th European Society for Biomedical Research on Alcoholism Congress. Crete, Greece. 8-11 octubre, 2017.

“Acetaldehyde and fenofibrate: a new old drug that markedly inhibits ethanol consumption” Karahanian E, Rivera-Meza M, Quintanilla ME, Muñoz D, Israel Y. ISBRA ESBRA-World Congress on Alcohol an Alcoholism. Berlin, Germany. 2-5 septiembre, 2016.

“The liver-brain axis: brain acetaldehyde and alcohol addiction” Israel Y, Quintanilla ME, Karahanian E, Rivera-Meza M, Morales P, Berrios-Carcamo P, Salinas-Luypaert C, Herrera-Marschitz M. ISBRA-ESBRA World Congress on Alcohol an Alcoholism. Berlin, Germany. 2-5 septiembre, 2016.

“Reinforcement by acetaldehyde-dopamine products leading to early ethanol intake shift to glutamate-related mechanisms to perpetuate intake. A new therapeutic option” Quintanilla ME, Israel Y, Karahanian E, Rivera-Meza M, Morales P, Herrera-Marschitz M. ISBRA-ESBRA World Congress on Alcohol an Alcoholism. Berlin, Germany. 2-5 septiembre, 2016.

“Fenofibrate –a lipid-lowering drug- increases blood acetaldehyde levels and reduces voluntary alcohol drinking in rats” Karahanian E, Rivera-Meza M, Quintanilla ME and Israel Y. 15th European Society for Biomedical Research on Alcoholism Congress. Valencia, Spain. 12-15 de septiembre de 2015.

“A clear paradox: aversive effects of liver-generated acetaldehyde but rewarding/reinforcing effects of acetaldehyde generated in the brain” Y. Israel, M.E. Quintanilla, E. Karahanian, M. Rivera-Meza, M. Herrera-Marschitz. 37th Annual Research Society on Alcoholism (RSA) Scientific Meeting. Bellevue, WA, USA. 21-25 de junio de 2014.

“Salsolinol, free of isosalsolinol, exerts ethanol-like motivational/sensitization effects leading to increases in ethanol intake” M.E. Quintanilla, M. Rivera-Meza, P. Berrios-Carcamo, D. Bustamante, M. Buscaglia, P. Morales, E. Karahanian, M. Herrera-Marschitz, Y. Israel. 37th Annual Research Society on Alcoholism (RSA) Scientific Meeting. Bellevue, WA, USA. 21-25 de junio de 2014.

“Gene therapy approaches in alcoholism: preclinical studies” Israel, Y; Quintanilla, ME; Karahanian, E; Tampier, L; Rivera-Meza, M; Morales, P; Herrera-Marschitz, M. Motivated Behaviors: Stress and Addiction: 2nd International Workshop, Valparaiso, Chile 15 al 17 de Noviembre, 2012.

“Combined inhibition of reward and conditioning prevents relapse in an animal model of alcohol dependence.” E. Karahanian, M.E. Quintanilla, L. Tampier, M. Rivera-Meza, M. Herrera-Marschitz, Y. Israel. 35th Annual Research Society on Alcoholism (RSA) Scientific Meeting. San Francisco, CA, USA. 23-27 de junio de 2012.

“Full inhibition of alcohol drinking in animals bred for high consumption” Yedy Israel, María Elena Quintanilla, Lutske Tampier, Eduardo Karahanian, Mario Rivera-Meza, Mario Herrera-Marschitz. The 6th International Symposium on ALPD and Cirrhosis. Fukuoka, Japan, October 20 and 21, 2011.

“Reward and conditioning: full dissociation by gene administration in an animal model of alcoholism.” ME Quintanilla, L. Tampier, E Karahanian, M Rivera, M Herrera-Marschitz, Y Israel. Meeting of the Latin-American Society for Biomedical Research on Alcoholism. Sao Paulo, Brazil. 31 marzo- 1 abril de 2011.

“Microinjection of an anti-catalase shRNA-coding lentiviral vector into brain ventral tegmental area (VTA) virtually abolishes ethanol intake by rats bred as high alcohol drinkers.” E. Karahanian; M.E. Quintanilla; L. Tampier; D. Bustamante; V. Gonzalez-Lira; P. Morales ; M. Herrera-Marschitz; Y. Israel
ISBRA World Congress: Current Topics and Innovations in Alcohol Research. 13-16 de septiembre de 2010, París, France.

National Meetings since 2010:

“Activation of melanocortin-4 receptor by a synthetic agonist inhibits ethanol-induced neuroinflammation in rats.” Flores-Bastías O., Gómez G. I., Orellana J. A., Karahanian E.” XL Congreso Anual de la Sociedad de Farmacología de Chile. Santa Cruz, November 13-16, 2018.

“Efectos perifericos y centrales en la reduccion del consumo de etanol inducida por fenofibrato” Rivera-Meza, M, Jerez, E, Muñoz, D, Quintanilla, ME, Salinas-Luypaert, C, Fernández, K, Karahanian, E. XXXIX Congreso Anual de la Sociedad de Farmacología de Chile. Puerto Varas, 22-25 de noviembre de 2017.

“Adquisición y mantenimiento de la ingesta de etanol: dos mecanismos” Quintanilla, M.E., Rivera-Meza, M., Karahanian, E., Berríos-Cárcamo, P., Salinas-Luypaert, C., Herrera? Marschitz, M; Israel, Y. XXXVIII Congreso Anual de la Sociedad de Farmacología de Chile. Castro, 26-29 de noviembre de 2016.

“Evaluación de fenofibrato como nuevo farmaco para el tratamiento del alcoholismo” Muñoz, D., Rivera-Meza, M., Quintanilla, M.E., Karahanian, E. XXXVIII Congreso Anual de la Sociedad de Farmacología de Chile. Castro, 26-29 de noviembre de 2016.

“El tratamiento con fenofibrato evita la preferencia de lugar condicionado por etanol y reduce el efecto de privación en ratas bebedoras UChB” Salinas-Luypaert, C., Jerez, E., Gallardo-Castillo, E.P., Rivera-Meza, M., Karahanian, E. XXXVIII Congreso Anual de la Sociedad de Farmacología de Chile. Castro, 26-29 de noviembre de 2016.

“Fenofibrato: un nuevo (y antiguo) farmaco que inhibe el consumo de alcohol” Karahanian, E., Rivera-Meza, M., Jerez, E., Muñoz, D., Quintanilla, M.E. LIX Reunion Anual de la Sociedad de Biología de Chile. Concón, 8-10 de noviembre de 2016.

“Fenofibrato -una droga hipolipemiante- incrementa los niveles sanguíneos de acetaldehído reduciendo la ingesta voluntaria de alcohol en ratas” Muñoz, D., Rivera-Meza, M., Quintanilla, M.E., Karahanian, E. LVIII Reunión Anual Sociedad de Biología de Chile. Puerto Varas, 23-25 de noviembre de 2015.

“Fenofibrato - agente reductor de lípidos que inhibe el consumo de alcohol” Karahanian, E. Simposio satélite sobre el alcoholismo, ASH, NASH y pancreatitis. Red Hospital Clínico de la Universidad de Chile. Santiago, 28 de septiembre de 2015

“La administración de salsolinol, libre de isosalsolinol, produce efectos motivacionales similares a etanol." Quintanilla, M.E.; Rivera?Meza, M.; Berrios, P; Bustamante, D; Buscaglia, M; Morales, P; Karahanian, E.; Herrera? Marschitz, M; Israel, Y. XXXVI Congreso Anual de la Sociedad de Farmacología de Chile. Chillán, 5-7 de noviembre de 2014.

“Alcohol: disociacion entre fenomeno de recompensa y de condicionamiento que mantiene su consumo” Israel, Y; Tampier, L; Quintanilla, M.E.; Karahanian, E.; Rivera?Meza, M.; Herrera? Marschitz, M. XXXIII Congreso Anual de la Sociedad de Farmacología de Chile. Olmué, 16-19 de noviembre de 2011.

“La inyeccion cerebral de un vector lentiviral anticatalasa a ratas bebedoras de alcohol suprime su consumo de etanol” Karahanian, E., Quintanilla, M.E., Tampier, L., Bustamante, D., Gonzalez-Lira V., Merino, V., Henríquez, A., Herrera-Marschitz, M., Israel, Y. XXXII Congreso Anual de la Sociedad de Farmacología de Chile. Valdivia, 3-6 de noviembre de 2010


Premios y Distinciones

  •   Beca de Doctorado

    CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS

    Chile, 1995

    PhD scholarship (CONICYT)


 

Article (27)

Fenofibrate (a PPAR-a Agonist) Administered During Ethanol Withdrawal Reverts Ethanol-Induced Astrogliosis and Restores the Levels of Glutamate Transporter in Ethanol-Administered Adolescent Rats
Use of short-chain fatty acids for the recovery of the intestinal epithelial barrier affected by bacterial toxins
Fenofibrate -a PPAR alpha agonist- increases alcohol dehydrogenase levels in the liver: implications for its possible use as an ethanol-aversive drug
Activation of Melanocortin-4 Receptor by a Synthetic Agonist Inhibits Ethanol-induced Neuroinflammation in Rats
Gene and cell therapy on the acquisition and relapse-like binge drinking in a model of alcoholism=> translational options.
Neuroinflammation produced by heavy alcohol intake is due to loops of interactions between Toll-like 4 and TNF receptors, peroxisome proliferator-activated receptors and the central melanocortin system=> A novel hypothesis and new therapeutic avenues.
Acquisition, Maintenance and Relapse-Like Alcohol Drinking=> Lessons from the UChB Rat Line.
Fenofibrate Administration Reduces Alcohol and Saccharin Intake in Rats=> Possible Effects at Peripheral and Central Levels
New Implications for the Melanocortin System in Alcohol Drinking Behavior in Adolescents=> The Glial Dysfunction Hypothesis.
Draft Genome sequence of a copper resistant marine bacterium, Pantoea agglomerans strain LMAE-2, a bacterial strain with potential use in bioremediation
Long-term inhibition of ethanol intake by the administration of an aldehyde dehydrogenase-2 (ALDH2)-coding lentiviral vector into the ventral tegmental area of rats
The "first hit" toward alcohol reinforcement=> role of ethanol metabolites.
Fenofibrate - A lipid-lowering drug - Reduces voluntary alcohol drinking in rats
Salsolinol, free of isosalsolinol, exerts ethanol-like motivational/sensitization effects leading to increases in ethanol intake
Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation
The Alcohol Deprivation Effect: Marked Inhibition by Anticatalase Gene Administration into the Ventral Tegmental Area in Rats
Reward and Relapse: Complete Gene-Induced Dissociation in an Animal Model of Alcohol Dependence
Ethanol as a Prodrug: Brain Metabolism of Ethanol Mediates its Reinforcing Effects
Purification and characterization of the antimicrobial peptide microcin N
Gene therapy reduces ethanol intake in an animal model of alcohol dependence
Aldehyde dehydrogenase (ALDH2) activity in hepatoma cells is reduced by an adenoviral vector coding for an ALDH2 antisense mRNA
Use of an acetaldehyde clamp" in the determination of low-K-M aldehyde dehydrogenase activity in H4-II-E-C3 rat hepatoma cells"
Gene and antisense delivery in alcoholism research
Cloning and molecular analysis of a cDNA and the Cs-mnp1 gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvermispora.
Structure and expression of a laccase gene from the ligninolytic basidiomycete Ceriporiopsis subvermispora.
Lip-like Genes in Phanerochaete Sordida and Ceriporiopsis Subvermispora, White Rot Fungi With No Detectable Lignin Peroxidase Activity
Isolation and partial purification of mitochondrial and cytosolic rhodanese from liver of normal and p-dimethylaminoazobenzene treated mice.

Letter (1)

PPAR? agonists reduce alcohol drinking: Do they act in the brain or in the liver?

Proyecto (11)

Disminución de la neuroinflamación por administración de un fármaco hipolipemiante en un modelo animal de consumo de alcohol.
Efectos protectores a largo plazo de un fármaco hipolipemiante sobre un modelo de consumo de alcohol tipo recaída
RESEARCH CENTER FOR THE STUDY OF ALCOHOL DRINKING BEHAVIOR IN ADOLESCENTS=> FROM MOLECULE TO THE BRAIN FUNCTION AND PERIPHERAL METABOLISM
A NEW PHARMACOLOGIC STRATEGY FOR THE TREATMENT OF ALCOHOLISM=> STUDIES IN ANIMAL MODELS
REWARDING MOLECULES GENERATED FROM ETHANOL IN THE BRAIN
Un nuevo tratamiento para el alcoholismo=> estudios en modelos animales
En busca de una terapia génica de por vida contra el alcoholismo
ETHANOL AS A PRODRUG
Instituto Milenio de Dinámica Celular y Biotecnología ICDB
Desarrollo de vectores adenovirales de última generación para una terapia génica contra el alcoholismo
MECANISMOS GENETICOS DE AVERSION AL ETANOL=> MODELOS CELULARES Y ANIMALES.

Review (2)

Activation of Melanocortin-4 Receptor Inhibits Both Neuroinflammation Induced by Early Exposure to Ethanol and Subsequent Voluntary Alcohol Intake in Adulthood in Animal Models: Is BDNF the Key Mediator?
Gene specific modifications unravel ethanol and acetaldehyde actions
25
Eduardo Karahanian

Académico Investigador

Facultad de Ciencias de la Salud

Universidad Autónoma de Chile

Santiago, Chile

13
Eduardo Karahanian

Professor

INSTITUTO DE CIENCIAS BIOMEDICAS

UNIVERSIDAD AUTONOMA DE CHILE

Santiago, Chile

9
Yedy Israel

Profesor Titular

Farmacologia Molecular y Clinica

UNIVERSIDAD DE CHILE, FACULTAD DE CS QUIMICAS Y FARMACÉUTICAS, LAB. FARMACOTERAPIA GÉNICA

Santiago, Chile

7
Maria Quintanilla

PROFESOR ASOCIADO

FARMACOLOGÍA MOLECULAR Y CLÍNICA

UNIVERSIDAD DE CHILE, FACULLTAD DE MEDICINA, INSTITUTO DE CIENCIAS BIOMÉDICAS, PROGRAMA DE FARMACOLOGÍA MOLECULAR Y CLÍNICA

Santiago, Chile

5
Mario Herrera-Marschitz

Profesor titular

Mol. & Clin. Pharmacology

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, PROGRAMA FARMACOLOGIA MOLECULAR & CLINICA, ICBM

Santiago, Chile

3
Paola Morales

Profesor Asociado

Programa Farmacología Molecular & Clínica, ICBM,

Universidad de Chile

Santiago, Chile

3
Katia Fernández

Académico - Profesor Asociado

Facultad de Salud y Odontología

Universidad Diego Portales

Santiago, Chile

2
Alvaro Lladser

Professor, Principal Investigator

FUNDACIÓN CIENCIA PARA LA VIDA

Santiago, Chile

1
Gino Corsini

Director Instituto Ciencias Biomédicas

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

1
Mario Tello

Académico

Biología

Universidad de Santiago de Chile

Santiago, Chile

1
Alonso Ferrer

Profesor Asistente

Universidad Mayor de Chile

Santiago, Chile

1
Marianne Buscaglia

Estudiante de Doctorado en Ciencias Biológicas, mención Genética Molecular y Microbiología

Genética Molecular y Microbiología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Gonzalo Encina

Investigador

Family Gen

Santiago, Chile

1
Juan Orellana

Associate Professor

Departamento de Neurología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Gonzalo Gomez

Profesor Asistente

Instituto de Ciencias de la Saluda

Universidad Autónoma de Chile

Santiago, Chile

1
Pablo Berríos

Asistente de investigación

Universidad del Desarrollo

Santiago, Chile