S200_denisse.valladares.jpg_thumb90

Denisse Mayara Valladares Ide

Assistant Professor

Universidad de O'Higgins

Rancagua, Chile

Líneas de Investigación


Skeletal Muscle Physiology Pathologies associated with skeletal muscle Nutrition and Exercise as non-pharmacological therapy

Educación

  •  Degree in biochemistry, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 2006
  •  Biomedical Science, UNIVERSIDAD DE CHILE. Chile, 2014
  •  Biochemestry, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 2007

Experiencia Académica

  •   Assistant Profesor Full Time

    UNIVERSIDAD FINIS TERRAE

    Medicina

    Santiago, Chile

    2016 - 2020

  •   Adjunct Assitance Professor Other

    UNIVERSIDAD PEDRO DE VALDIVIA

    Medicina

    Santiago, Chile

    2009 - 2014

  •   Auxiliary Professor Other

    UNIVERSIDAD DE CHILE

    Odontología

    Santiago, Chile

    2011 - 2012

  •   Assistant Profesor Full Time

    Universidad de O'Higgins

    Instituto de Ciencias de la Salud

    Rancagua, Chile

    2020 - A la fecha

  •   Postdoctoral fellowship Full Time

    UNIVERSIDAD DE CHILE

    Facultad de Ciencias Químicas y Farmacéuticas

    Santiago, Chile

    2014 - 2016

Experiencia Profesional

  •   LABORATORY ASSISTANT Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Chile

    2007 - 2008

  •   Adjunct Assitance Professor Other

    Universidad Pedro de Valdivia

    Chile

    2009 - 2014

  •   LABORATORY ASSISTANT Part Time

    Universidad de Chile Facultad de Medicina

    Chile

    2008 - 2012

  •   Postdoctoral Fellowship Full Time

    Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile

    Santiago, Chile

    2014 - 2016

Formación de Capital Humano


THESIS DIRECTOR

MASTER
2019 Master in Health Science, Student: Jonathan Ríos, Title: “Efecto de 6 semanas de ayuno intermitente con entrenamiento aeróbico hiit sobre la composición corporal y perfil lipídico en sujetos sobrepeso u obesos: estudio piloto”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2019 Master in Health Science, Student: Mauro Tuñón, Title: “Activación de la autofagia durante diferentes intensidades de ejercicio aeróbico en jóvenes y adultos mayores ”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2019 Master in Health Science, Student: Jonathan Ríos, Title: “Efecto de 6 semanas de ayuno intermitente con entrenamiento aeróbico sobre la composición corporal y perfil lípidico en sujetos obesos u obesos: estudio piloto.”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2019 Master in Clinical Physiology of Exercise, Student: Juan Pablo Quezada, Title: “Efecto del entrenamieno sobre la funcionalidad y calidad de vida de pacientes con transplante hepático: Revisión sistemática”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2019 Master in Health Science, Student: Alexander Pizarro, Title: “Respuesta de la hormona del crecimiento al entrenamiento de oclusión vascular”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2018 Master in Health Science, Student: David Cahmi, Title: “Efectos del programa de entrenamiento escolar en el control de la obesidad en adolescentes de primer año de educación media”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2018 Master in Health Science, Student: Francisco Salfate, Title: “Efecto Del Rodillo Automasajeador Sobre La Glicemia En Sujetos Obesos Y Pacientes Diabéticos Tipo 2”, Escuela de Kinesiología, Facultad de Medicina, Universidad Finis Terrae.

2017 Master in Health Science, Student: Rosemary Arenas, Title: “Efecto del entrenamiento de resistencia sobre la inflamación sistémica, resistencia a la insulina y vías de señalización de TLR4 en sujetos normopeso y obesos”, Escuela de Kinesiología, Universidad Finis Terrae.

2017 Master in Health Science, Student: Mario Elgueta, Título: “Efectos del ejercicio aeróbico sobre la disminución de los niveles de il-6 en plasma sanguíneo y mejora de la sensibilidad a la insulina en personas obesas”, Escuela de Kinesiología, Universidad Finis Terrae

2017 Master in Clinical Physiology of Exercise, Student: Manuel Vasquez: “Efecto del ejercicio físico en el control glicémico del paciente diabéticos tipo 1”, Escuela de Kinesiología, Universidad Finis Terrae

UNDERGRATUATE
2014 Medical and Clinical Laboratory Technologist, Student: Giomar Itriago, Título: “Caracterización de complejos multiproteicos e enfluencia de Nifedipino sobre la apoptosis en fibras musculares de ratones Mdx”, Escuela de Tecnología Médica, Universidad de Chile.


Difusión y Transferencia


2012- 2016 PAR-EXPLORA RM Activities along with ACCDIS-FONDAP and CEMC_FONDAP

2020-2021 PAR-EXPLORA O'HIGGINS: Academic Advisor

2020-2021 POLITICAL COMMITTEE ON EQUITY AND GENDER

2019-2021 Chilean Society Physiological Sciences Directory and Treasurer

PRESENTATIONS AT NATIONAL AND INTERNATIONAL CONGRESSES:
2020
- Denisse Valladares Maria José Bravo, Ana Carvajal, Oscar Araneda, Marcelo Tuesta, Alvaro Reyes, Luis Peñailillo
Effect of Eccentric and Concentric Cycling Changes over pulmonary and plasma Oxidative Stress and Inflammation in Stable COPD
XXXV Reunión Anual de la Sociedad Chilena de Ciencias
Fisiológicas, Virtual, Chile.
- Jonathan Ríos, Matías Monsalves Álvarez Carlos Puebla, Denisse Valladares
Effects of intermittent fasting with high-intensity interval training (HIIT) over body composition,
lipid profile, and food consumption on obese subjects: A Pilot Study.
XXXV Reunión Anual de la Sociedad Chilena de Ciencias
Fisiológicas, Virtual, Chile.
2019
- Tuñón M, Zbinden H, Valladares D. Impact of different intensity of aerobic exercise in autophagy in
young health adults. XXXIV Reunión Anual de la Sociedad Chilena de Ciencias Fisiológicas, Santa
Cruz, Chile.
- Valladares D, Tuñón M, Flores M, Zbinden-Foncea H. NOX2 activity regulates autophagy in an agedependent
manner in human myotubes. XXXIV Reunión Anual de la Sociedad Chilena de Ciencias
Fisiológicas, Santa Cruz, Chile.
2018
- Hermann Zbinden-Foncea, Marcelo Flores-Opazo, Denisse Valladares Ide, Cristóbal Muñoz-
Medina, Luis Peñailillo. Effects of human conditioned serum and electric pulse stimulation on TLR4
signaling on human primary myotubes. XXXII Annual Meeting of the Sociedad de Biología Celular de
Chile, CHILE, Puerto Varas.
2017
- Symposium Titled: Skeletal Muscle Function: Focus on Physiological Signaling for Autophagy,
Mitochondrial Ca2+ Control and GLUT4 Trafficking. Speackers: Dr. Denisse Valladares “IP3
receptor modulates autophagy, mitochondrial dynamics and mitophagy in skeletal muscle: New
insights muscular dystrophy treatment”. XXXII Reunión Anual de la Sociedad Chilena de Ciencias
Fisiológicas, Pucón, Chile.
- Denisse Valladares, Luis Peñailillo, , Ariel Contreras-Ferrat, Herman Zbinden-Foncea Activation of
hypertrophy and inflammatory signalling pathways after repeated bouts of eccentric cycling in
humans.. Cell Symposia: Exercise Metabolism 2017, Gothenburg, Sweden.
2016
- Valladares, Y. Utreras-Mendoza, C. Morales, C. Campos, F. Westermeier, S. Lavandero, E. Jaimovich.
IP3 Receptor Determines Increased Basal Mitochondrial Calcium and Autophagy Regulation in Mdx
Skeletal Muscle. Experimental Biology 2016: ASBMB, USA, San Diego.
- A.E. Contreras-Ferrat, A. Díaz-Vegas, C. Campos, Y. Utreras, D. Valladares, G. Rosales, P. Llanos, M.
Arias, E. Jaimovich. Insulin-Dependent Glucose Uptake Is Regulated by Mitochondrial Ca2+ Handling
in Skeletal Muscle Fibers. Experimental Biology 2016: ASBMB, USA, San Diego.
2015
- C. Henriquez-Olguin, D. Valladares, A. Diaz-Vegas, Y. Utreras-Mendoza, C. Campos, P. Llanos, A.
Contreras-Ferrat, A. Espinosa, F. Altamirano, M. Arias Calderón and E. Jaimovich. NOX2-Dependent
ROS Production Is Essential for the Response to Endurance Exercise in Skeletal Muscle.
Experimental Biology 2015: ASBMB, USA, Boston.
- C. Henriquez-Olguín, H. Cerda, A. Díaz-Vegas, Y. Utreras, M. Arias, A. Contreras, P. Llanos, F.
Altamirano, E. Jaimovich, D. Valladares. NOX2 inhibition alters adaptive signaling induced by
endurance exercise in skeletal muscle. Cell Symposia: Exercise Metabolism 2015, Holanda,
Ámsterdam.
2014
- Denisse Valladares, Francisco Altamirano, Carlos Henríquez-Olguín, Alexis Díaz-Vegas, Giomar
Intriago, Ariel Contreras-Ferrat, Sonja Buvinic, José R. López, Paul Allen, Enrique Jaimovich. ATP
Signaling Complex Is Altered In Muscular Dystrophy And Was Partly Recovered After Nifedipine
Treatment. Experimental Biology 2013: ASBMB, USA, San Diego.
2013
- Valladares D, Altamirano F, Henríquez C, López JF, Allen PD, Jaimovich E. Nifedipine Treatment
Reduces ATP Release, Cell Death And Damage In Dystrophic Mdx Muscles. XII Meeting de
PanAmerican Bochemistry & Molecular Biology Societies XLIX Annual Meeting of the Argentinean
Society of Biochemistry and Molecular Biology (SAIB), XXXVI the Annual Meeting of the Society of
Biochemistry and Molecular Biology of Chile, the 4th Meeting of the Latin L American Protein
Society and the LVI Annual Meeting of the Society of Biology of Chile, Puerto Varas.
2012
- Valladares D., Almarza G., Jaimovich E., Muscle Fiber Degeneration Induced By Atp Stimulation In
A Dystrophic Mouse Model, XXVI Annual Meeting of the Sociedad de Biología Celular de Chile,
CHILE, Puerto Varas.
- Valladares D., Almarza G, Jaimovich E., ATP release is altered in a mouse model for Duchenne
muscular dystrophy and signals for proteins that promote cell death, Experimental Biology 2012:
ASBMB, ESTADOS UNIDOS DE AMERICA, San Diego.
2011
- Valladares D., Almarza G., Jaimovich E., Differential Gene Expression Between Normal And Mdx
Mouse Fibers Induced By ATP Signaling, XXV Annual Meeting of the Sociedad de Biología Celular de
Chile, CHILE, Puerto Varas.
2010
- Valladares D., Figueroa R., Buvinic S., Jaimovich E., Differences In ATP Signalling Induce By
Electrical Stimulation Betewen Normal And Mdx Mouse Fibers, Society For Cell Biology 2010 Annual
Meeting, Chile, Pucón
2009
- Valladares D., Buvinic S., Sobrevia L. and Jaimovich E., Adenosine Stimulates Intracellular Calcium
Release In Skeletal Muscle Cells., Gordon Research Conference on Muscle: Excitation / Contraction
Coupling, ESTADOS UNIDOS DE AMERICA, New Hapshire.
- Valladares D, Buvinic, S, Figueroa, R and Jaimovich, E., Immunolocalization Of Purinergic Receptors
In Adult Mouse Fibers: Receptor Activation Produces Calcium Transients, LII Reunión Anual
Sociedad De Biología De Chile, CHILE, Pucón


Premios y Distinciones

  •   Course atended and travel award

    INSTITUT PASTEUR

    Francia, 2011

    Beca para asistir al curso de Biología Molecular y Celular, durante un mes en el Instituto Pasteur

  •   Travel and assistance scholarship

    UNIVERSIDAD DE LA REPUBLICA URUGUAY

    Uruguay, 2009

    Curso y mini-simposio internacional: "Physiology of membrane Ion Transport"

  •   Experimental Biology 2014 travel award

    ASBMB society

    Estados Unidos, 2014

    Asistencia a congreso Experimental Biology

  •   Experimental Biology 2012 travel award

    ASBMB society

    Chile, 2012

    Asistencia a congreso Experimental Biology

  •   Best Poster Presentation

    Sociedad de Biología Celular de Chile

    Chile, 2011

    Presentación de mejor Poster de Doctorado en el congreso XXV Annual Meeting Sociedad de Biología Celular de Chile

  •   Tesis Scholarship

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 2011

    Apoyo monetario para la realización de la tesis de doctorado

  •   PhD Grant

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 2008

    Beca para la realización de doctorado en la Universidad de Chile


 

Article (16)

Changes in pulmonary and plasma oxidative stress and inflammation following eccentric and concentric cycling in stable COPD patients
High extracellular ATP levels released through pannexin-1 channels mediate inflammation and insulin resistance in skeletal muscle fibres of diet-induced obese mice
NLRP3 inflammasome participation in obesity related low-grade inflammation and insulin resistance in skeletal muscle
Moderate Exercise in Spontaneously Hypertensive Rats Is Unable to Activate the Expression of Genes Linked to Mitochondrial Dynamics and Biogenesis in Cardiomyocytes
Activation of protein synthesis, regeneration, and MAPK signaling pathways following repeated bouts of eccentric cycling
Changes in oxidative stress, inflammation and muscle damage markers following eccentric versus concentric cycling in older adults
Defective fasting-induced PKA activation impairs adipose tissue glycogen degradation in obese Zucker rats
IP3 receptor blockade restores autophagy and mitochondrial function in skeletal muscle fibers of dystrophic mice
Mitochondrial Calcium Increase Induced by RyR1 and IP3R Channel Activation After Membrane Depolarization Regulates Skeletal Muscle Metabolism
Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle
GSK-3 beta/NFAT Signaling Is Involved in Testosterone-Induced Cardiac Myocyte Hypertrophy
NOX2 inhibition impairs early muscle gene expression induced by a single exercise bout
Altered ROS production, NF-kappa B activation and interleukin-6 gene expression induced by electrical stimulation in dystrophic mdx skeletal muscle cells
Electrical Stimuli Are Anti-Apoptotic in Skeletal Muscle via Extracellular ATP. Alteration of This Signal in Mdx Mice Is a Likely Cause of Dystrophy
Nifedipine Treatment Reduces Resting Calcium Concentration, Oxidative and Apoptotic Gene Expression, and Improves Muscle Function in Dystrophic mdx Mice
Adenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli

Abstract (4)

ATP signaling complex is altered in muscular dystrophy and was partly recovered after nifedipine treatment
Cav1.1 controls ATP release to elicit gene expression in adult muscle fibers
Nifedipine Treatment Improves Muscle Function in Mdx Mice
ATP release is altered in a mouse model for Duchenne muscular dystrophy and signals for proteins that promote cell death

BookSection (1)

Altered Gene Expression Pathways in Duchenne Muscular Dystrophy

Proyecto (6)

Exercise stimulates autophagy by downregulation of NOX2 activity improving young and aged human skeletal muscle function
Modulación de la autofagia por el receptor de inositol trifosfato (IP3R) en distrofia muscular de Duchenne
BIOENERGETIC PATHWAYS ACTIVATED BY INTRACELLULAR CALCIUM SIGNALS IN CARDIAC HYPERTROPHY INDUCED BY ANDROGENS
A NEW ROLE FOR DYSTROPHIN IN EXCITATION-TRANSCRIPTION COUPLING IN SKELETAL MUSCLE
ALTERACIONES DE LA FUNCION CELULAR EN EL GLOMERULO RENAL INDUCIDAS POR ADENOSINA=> IMPLICANCIAS EN LA PATOGENESIS Y EL TRATAMIENTO DE LA NEFROPATIA DIABETICA.
"RELACION DEL SINDROME DIABETICO CON LA EXPRESION Y LOCALIZACION CELULAR DE GLICOGENO SINTASA, FOSFOENOLPIRUVATO CARBOXIQUINASA Y FRUCTOSA-1, 6-BISFOSTASA, ENZIMAS CLAVES EN LA HOMEOSTASIS DE LA GLUCOSA"

Review (1)

Do adenosine receptors offer new therapeutic options for diabetic nephropathy?
23
Denisse Valladares

Assistant Professor

Universidad de O'Higgins

Rancagua, Chile

9
Enrique Jaimovich

Full Professor

ICBM

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

6
Rody San Martín

Professor

Sciences Faculty

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

5
Alejandro Yanez

Full professor

Faculty of Science

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

5
Mariana Casas

Assistant Professor

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

3
Hermann Zbinden

Associate Professor

Kinesiology

UNIVERSIDAD FINIS TERRAE

Santiago, Chile

3
Sonja Buvinic

Associate Professor

Institute for Research in Dental Sciences

Universidad de Chile

Santiago, Chile

2
YILDY UTRERAS

Postdoctoral researcher

Universidad de Los Andes

santiago, Chile

2
Luis Penailillo

Profesor Asociado

Facultad de Ciencias de la Rehabilitación

Universidad Andrés Bello

Santiago, Chile

2
Cristian Campos

Entrenamiento Post Doctorado

Fisiología

Universidad de Chile

Santiago, Chile

1
LUIS SOBREVIA

Professor

Division of Obstetrics and Gynaecology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

1
Claudia Quezada

Full Professor

Institute of Biochemistry and Microbiology

Universidad Austral de Chile

Valdivia, Chile

1
Alexis Diaz

PhD stundent

Fisiología

universidad de Chile

Santiago, Chile

1
Cesar Oyarce Diaz

Posdtoc

MMB

UMCG

Groningen, Holanda

1
Ariel Contreras

assistance professor

Institute for Research in Dental Science

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Carla Basualto

Jefe de Departamento - Académico

Departamento de Ciencias de la Salud

Universidad de Aysén

Coyhaique, Chile

1
MARCELO FLORES

Académico Investigador

Kinesiología

Universidad Finis Terrae

Santiago, Chile

1
Hugo Marambio

JEFE

TRAUMATOLOGIA

CLINICA SANTA MARIA

SANTIAGO, Chile

1
Paola Llanos

Profesor Asistente

Instituto de Investigacion en Ciencias Biomédicas

FACULTAD DE ODONTOLOGIA. UNIVERSIDAD DE CHILE

Santiago, Chile

1
Gonzalo Jorquera

Profesor Auxiliar

Instituto de Fisiología

Universidad de Valparaíso

Valparaíso, Chile