Man

Barbara Anne Andrews Farrow

FULL PROFESSOR

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

Líneas de Investigación


Protein Engineering. Metabolic Engineering. Extremophiles.

Educación

  •  BIOCHEMISTRY, UNIVERSITY OF LONDON. Reino Unido, 1974
  •  BIOCHEMICAL ENGINEERING, UNIVERSITY OF LONDON. Chile, 1985

Experiencia Académica

  •   RESEARCH ASSOCIATE Full Time

    COLUMBIA UNIVERSITY

    NEW YORK, Estados Unidos

    1982 - 1986

  •   SENIOR RESEARCH ASSOCIATE Full Time

    UNIVERSITY OF READING

    Reino Unido

    1987 - 1994

  •   ASSOCIATE PROFESSOR Full Time

    UNIVERSIDAD DE CHILE

    PHYSICAL AND MATHEMATICAL SCIENCES

    Chile

    1996 - 2004

  •   FULL PROFESSOR Full Time

    UNIVERSIDAD DE CHILE

    PHYSICAL AND MATHEMATICAL SCIENCES

    Chile

    2004 - A la fecha

Formación de Capital Humano


In the past 3 years the thesis directed are:
6 Undergraduate: Adriana López (2010), Gabriela Sandoval (2011), Daniela Vaisman (2011), Mauricio Azocar (2011), Alessandra Baldecchi (2013), Camila Bay (2013)
4 Magíster: Gabriela Sandoval (2011), Daniela Vaisman (2011), Camila Wilkens (2011), Mauricio Azocar (2011)
2 PhD: Juan Matias Saavedra (2011), María Paz Merino (2012)


Difusión y Transferencia


Patentes
Ortogados
US Patent: “Protein and Nucleic Acid Sequence Encoding a Krill-Derived Cold Adapted Trypsin-Like Activity Enzyme”, US 7,202,074 B2, Apr. 10, 2007. Inventors: Asenjo J.A., Andrews, B.A.,Reyes, F., Salamanca M., Burzio L.
US Patent: “Materials and Methods for Regulating Process Formation in Cell Culture”, US 7,323,333 B2, Jan. 29, 2008, Inventors: Caviedes, P., Caviedes, R., Freeman, T.B., Asenjo J.A., Andrews, B.A., Sepulveda D., Arriagada,C., Rivera, J.S.
US Patent: “Methods for Cell Therapy”, US 8,252,279 B2, Aug. 28, 2012, Inventors: Caviedes, P., Caviedes, R., Freeman, T.B., Asenjo J.A., Andrews, B.A., Sepulveda D., Arriagada,C., Rivera, J.S.

Solicitados
US Patent: “Protein and DNA Sequence Encoding a Cold Adapted Subtilisin-Like Activity”, US Provisional Application 60/954/198, August 6, 2007. Inventors: Asenjo, J.A., Andrews, B.A.,Reyes, Acevedo, J.P., Burzio L.

US Patent: “Protein and DNA Sequence Encoding a Cold Adapted Xylanase”, US Provisional Application 61/150, 545, February 6, 2009. Inventors: Asenjo, J.A., Andrews, B.A., Acevedo, J.P., Parra, L. Burzio L.



 

Article (107)

Heterologous expression and biochemical characterization of a novel cold-active alpha-amylase from the Antarctic bacteria Pseudoalteromonas sp. 2-3
The Polyextreme Ecosystem, Salar de Huasco at the Chilean Altiplano of the Atacama Desert Houses Diverse Streptomyces spp. with Promising Pharmaceutical Potentials
Use of genome-scale models to get new insights into the marine actinomycete genus Salinispora
Amycolatopsis vastitatis sp nov., an isolate from a high altitude subsurface soil on Cerro Chajnantor, northern Chile
Analysis of metabolic networks of Streptomyces leeuwenhoekii C34 by means of a genome scale model: Prediction of modifications that enhance the production of specialized metabolites
Blastococcus atacamensis sp nov., a novel strain adapted to life in the Yungay core region of the Atacama Desert
Geodermatophilus chilensis sp nov., from soil of the Yungay core-region of the Atacama Desert, Chile
High altitude, hyper-arid soils of the Central-Andes harbor mega-diverse communities of actinobacteria
Metabolic modelling and flux analysis of microorganisms from the Atacama Desert used in biotechnological processes
Relevance of Local Flexibility Near the Active Site for Enzymatic Catalysis: Biochemical Characterization and Engineering of Cellulase Cel5A From Bacillus agaradherans
AAV Gene Therapy for Alcoholism: Inhibition of Mitochondrial Aldehyde Dehydrogenase Enzyme Expression in Hepatoma Cells
Purification of adenoviral vector serotype 5 for gene therapy against alcoholism using anion exchange chromatography
Streptomyces asenjonii sp nov., isolated from hyper-arid Atacama Desert soils and emended description of Streptomyces viridosporus Pridham et al. 1958
Acidithiobacillus ferrooxidans's comprehensive model driven analysis of the electron transfer metabolism and synthetic strain design for biomining applications
Characterization of Ferroplasma acidiphilum growing in pure and mixed culture with Leptospirilum ferriphilum
Analyzing redox balance in a synthetic yeast platform to improve utilization of brown macroalgae as feedstock
Genome-scale reconstruction of Salinispora tropica CNB-440 metabolism to study strain-specific adaptation
Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii
Identification of lipase encoding genes from Antarctic seawater bacteria using degenerate primers: Expression of a cold-active lipase with high specific activity
Production of Cell-Penetrating Peptides in Escherichia coli Using an Intein-Mediated System
Stoichiometric Model and Flux Balance Analysis for a Mixed Culture of Leptospirillum ferriphilum and Ferroplasma acidiphilum
The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid pSLE1 and linear plasmid pSLE2
Design and implementation of a high yield production system for recombinant expression of peptides
Generation of an atlas for commodity chemical production in Escherichia coli and a novel pathway prediction algorithm, GEM-Path
Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease
Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease.
Cold Adaptation, Ca2+ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications
Cold Adaptation, Ca2+ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications
Streptomyces bullii sp nov., isolated from a hyper-arid Atacama Desert soil
Streptomyces bullii sp. nov., isolated from a hyper-arid Atacama Desert soil.
Aqueous two-phase systems for protein separation: Phase separation and applications
Aqueous two-phase systems for protein separation=> phase separation and applications.
Continuous modeling of metabolic networks with gene regulation in yeast and in vivo determination of rate parameters
Continuous modeling of metabolic networks with gene regulation in yeast and in vivo determination of rate parameters.
Elution relationships to model affinity chromatography using a general rate model
Elution relationships to model affinity chromatography using a general rate model.
Hydrophobic interaction chromatography for purification of monoPEGylated RNase A
Hydrophobic interaction chromatography for purification of monoPEGylated RNase A.
Streptomyces atacamensis sp nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Chile
Streptomyces atacamensis sp. nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Chile.
Streptomyces deserti sp nov., isolated from hyper-arid Atacama Desert soil
Streptomyces deserti sp. nov., isolated from hyper-arid Atacama Desert soil.
Aqueous two-phase systems for protein separation=> a perspective.
Chaxamycins A-D, bioactive ansamycins from a hyper-arid desert Streptomyces sp.
Chaxamycins A-D, Bioactive Ansamycins from a Hyper-arid Desert Streptomyces sp.
Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment
Diverse metabolic profiles of a Streptomyces strain isolated from a hyper-arid environment.
Identification of Transcription Factors Perturbed by the Synthesis of High Levels of a Foreign Protein in Yeast Saccharomyces cerevisiae
Identification of transcription factors perturbed by the synthesis of high levels of a foreign protein in yeast Saccharomyces cerevisiae.
Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to predict structure-function related mutations in proteins
Mutagenesis Objective Search and Selection Tool (MOSST)=> an algorithm to predict structure-function related mutations in proteins.
Extension of the selection of protein chromatography and the rate model to affinity chromatography
Metabolic Flux Analysis of Embryonic Stem Cells Using Three Distinct Differentiation Protocols and Comparison to Gene Expression Patterns
Stoichiometric Model and Metabolic Flux Analysis for Leptospirillum ferrooxidans
Stoichiometric model and metabolic flux analysis for Leptospirillum ferrooxidans.
Theoretical and Experimental Evaluation of Hydrophobicity of Proteins to Predict their Partitioning Behavior in Aqueous Two Phase Systems: A Review
Viral vectors for the treatment of alcoholism: Use of metabolic flux analysis for cell cultivation and vector production
Viral vectors for the treatment of alcoholism=> Use of metabolic flux analysis for cell cultivation and vector production
Viral vectors for the treatment of alcoholism=> use of metabolic flux analysis for cell cultivation and vector production.
A Stoichiometric Model of Acidithiobacillus ferrooxidans ATCC 23270 for Metabolic Flux Analysis
A stoichiometric model of Acidithiobacillus ferrooxidans ATCC 23270 for metabolic flux analysis.
Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile
Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile.
Global gene expression in recombinant and non-recombinant yeast Saccharomyces cerevisiae in three different metabolic states
Global gene expression in recombinant and non-recombinant yeast Saccharomyces cerevisiae in three different metabolic states
Modeling heterocyst pattern formation in cyanobacteria
Protein purification using chromatography: selection of type, modelling and optimization of operating conditions
Cloning and fusion expression of a cold-active lipase from marine Antarctic origin
Cloning of complete genes for novel hydrolytic enzymes from Antarctic sea water bacteria by use of an improved genome walking technique
Cloning of complete genes for novel hydrolytic enzymes from Antarctic sea water bacteria by use of an improved genome walking technique.
Comparative Transcriptional Analysis of Embryoid Body Versus Two-Dimensional Differentiation of Murine Embryonic Stem Cells
Comparative transcriptional analysis of embryoid body versus two-dimensional differentiation of murine embryonic stem cells.
Prediction of the partitioning behaviour of proteins in aqueous two-phase systems using only their amino acid composition
Prediction of the partitioning behaviour of proteins in aqueous two-phase systems using only their amino acid composition.
A discrete mathematical model applied to genetic regulation and metabolic networks
Mathematical modeling of elution curves for a protein mixture in ion exchange chromatography and for the optimal selection of operational conditions
Correlation for the partition behavior of proteins in aqueous two-phase systems: Effect of surface hydrophobicity and charge
A mixed mechanistic-electrostatic model to explain pH dependence of glycosyl hydrolase enzyme activity
EFFECT OF ELECTROSTATIC ENERGY ON PARTITIONING OF PROTEINS IN AQUEOUS TWO-PHASE SYSTEMS
Optimal process synthesis for the production of multiple recombinant proteins
Metabolic analysis of the synthesis of high levels of intracellular human SOD in Saccharomyces cerevisiae rhSOD 2060 411 SGA122
Study of recombinant micro-organism populations characterized by their plasmid content per cell using a segregated model
Kinetic model of BiP- and PDI-mediated protein folding and assembly
Phase separation rates of aqueous two-phase systems: Correlation with system properties
Refractive index, density, and viscosity in the NaNO 3 + H 2O + poly(ethylene glycol) system at various temperatures
Influence of molecular weight of the polymer on the liquid-liquid equilibrium of the poly(ethylene glycol) + NaNo 3 + H 2O system at 298.15 K
Isolation, purification and preliminary characterization of cryophilic proteases of marine origin
Liquid-liquid and liquid-liquid-solid equilibrium in Na2CO3-PEG-H2O
Liquid-liquid and solid-liquid equilibria of the poly(ethylene glycol) plus sodium sulfate plus water system at 298.15 K
Metabolic control analysis of monoclonal antibody synthesis
Drowning-out crystallisation of sodium sulphate using aqueous two-phase systems
Effect of the growth conditions on the synthesis of a recombinant beta-1,4-endoglucanase in continuous and fed-batch culture
Model for the partition of metal ions in aqueous two-phase systems
Fedbatch fermentations of Bacillus subtilis ToC46 (pPFF1) for the synthesis of a recombinant ?-1,3-glucanase: Experimental study and modelling
Aqueous two-phase systems for protein separation studies on phase inversion
On the kinetics of phase separation in aqueous two-phase systems
Yeast cell permeabilizing ?-1,3-glucanases: A tool for the integration of downstream processes and metabolic engineering applications to yeast
Correlations for the partition behavior of proteins in aqueous two-phase systems: Effect of overall protein concentration
Hydrophobic partitioning of proteins in aqueous two-phase systems
Mathematical modeling of the synthesis of a cloned lytic ?-1,4-endoglucanase in Bacillus subtilis
Partitioning and purification of monoclonal antibodies in aqueous two-phase systems
Protein partitioning equilibrium between the aqueous poly(ethylene glycol) and salt phases and the solid protein phase in poly(ethylene glycol)-salt two-phase systems
Extension of the selection of protein chromatography and the rate model to affinity chromatography.
Metabolic flux analysis of embryonic stem cells using three distinct differentiation protocols and comparison to gene expression patterns.
Metabolic flux analysis of embryonic stem cells using three distinct differentiation protocols and comparison to gene expression patterns.
Mutagenesis Objective Search and Selectio Tool (MOSST)
Protein purification using chromatography=> selection of type, modelling and optimization of operating conditions.

ConferencePaper (1)

Optimization and design considerations of two-phase continuous protein separation

Patent (7)

Methods for cell therapy
Protein and DNA sequence encoding a cold adapted xylanase
Protein and DNA sequence encoding a cold adapted-subtilisin-like activity
Protein and DNA Sequence Encoding a Cold Adapted Subtilysin-like Activity
Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
Materials and Methods for Regulating Process Formation in Cell Culture
Method of Preparing a Yeast-cell lytic Enzyme System

Review (3)

The gifted actinomycete Streptomyces leeuwenhoekii
Aqueous two-phase systems for protein separation: A perspective
Challenges and trends in bioseparations
98
Barbara Andrews

FULL PROFESSOR

CHEMICAL ENGINEERING AND BIOTECHNOLOGY

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

68
Juan Asenjo

Professor

CHEMICAL AND BIOTECHNOLOGY ENGINEERING

University of Chile

SANTIAGO, Chile

9
Carolina Shene

TITULAR PROFESSOR

CHEMICAL ENGINEERING

UNIVERSIDAD DE LA FRONTERA

TEMUCO, Chile

9
Jean Castro

Curador

Banco de Recursos Genéticos Microbiano

Instituto de Investigaciones Agropecuarias

Chillán, Chile

6
Valeria Razmilic

Investigadora postdoctoral

Centro de Biotecnología y Bioingeniería

Universidad de Chile

Santiago, Chile

6
Vida Rodríguez

Investigadora Postdoctoral

Universidad de Chile

Santiago, Chile

6
Maria Salazar

Profesora Asociada

UNIVERSIDAD DE CHILE, FAC. DE CIENCIAS FÍSICAS Y MATEMATICAS DEPTO. INGENIERÍA QUÍMICA

Santiago, Chile

4
loreto parra

Assitant Professor

Chemical and Bioprocesses Engineering

Pontificia Universidad Católica de Chile

Santiago, Chile

4
Alvaro Olivera

Principal Investigator and Assistant Professor

Chemical Engineering and Biotechnology

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Ivan Rapaport

Associate Professor

Mathematical Engineering

Universidad de Chile

Santiago, Chile

3
Teófilo Graber

Académico. Profesor Titular

Ingeniería Química

UNIVERSIDAD DE ANTOFAGASTA

Antofagasta, Chile

3
Maria Taboada

Profesor Titular

Facultad de Ingeniería

UNIVERSIDAD DE ANTOFAGASTA

Antofagasta, Chile

2
Anamaria Sanchez

Investigadora

Centre for Biotechnology and Bioengineering

Santiago, Chile

2
Ziomara Gerdtzen

Profesora Asociada

Departamento de Ingeniería Química, Biotecnología y Materiales

Universidad de Chile

Santiago, Chile

2
Pablo Caviedes

Full professor

ICBM

PROGRAMA DE FARMACOLOGÍA MOLECULAR Y CLÍNICA, ICBM, FAC. DE MEDICINA, U. DE CHILE

Santiago, Chile

2
Gabriela Sandoval

Jefe de carrera

Departamento de Ingeniería Química

Universidad Tecnológica Metropolitana

Santiago, Chile

2
Pablo Moisset de Espanés

Investigador asociado

FCFM

CeBiB, Universidad de Chile

Santiago, Chile

2
Daniela Vaisman

Ingeniera de Proyectos

Centro de Biotecnología y Bioingeniería

Santiago, Chile

1
Alejandro Buschmann

FULL PROFESSOR

UNIVERSIDAD DE LOS LAGOS

PUERTO MONTT, Chile

1
Eric Goles

Full Professor

Facultad de Ingeniería y Ciencias

Universidad Adolfo Ibáñez

Santiago, Chile

1
Julio Aracena

Full Professor

Ingeniería Martemática

Universidad de Concepción

Concepción, Chile

1
Alicia Lucero

Académico e Investigador

Facultad de Ingeniería, Ciencia y Tecnología

Universidad Bernardo O'Higgins

Santiago, Chile

1
Angela Cintolesi Makuc

Postdoctorado

Centro de Modelamiento Matemático

Universidad de Chile

Santiago, Chile

1
José Salgado

Assistant Professor

Chemical Engineering, Biotechnology and Materials

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Maria Lienqueo

ASSOCIATE PROFESSOR

OF CHEMICAL AND BIOTECHNOLOGY ENGINEERING

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Giannina Espina

Research Scientist

Fundacion Biociencia

Santiago, Chile

1
Axel Osses

Profesor Titular

Departamento de Ingeniería Matemática

Universidad de Chile

Santiago, Chile

1
CESAR RAMIREZ

Assistant Professor

Institute for Biological and Medical Engineering

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
Cristina Dorador

PROFESSOR

Biotechnology

UNIVERSIDAD DE ANTOFAGASTA

ANTOFAGASTA, Chile

1
Carlos Cortés

Research assistant

University of Antofagasta

Antofagasta, Chile