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Igor Alejandro Pacheco Cruz

Profesor Asistente

INTA - Universidad de Chile

Santiago, Chile

Líneas de Investigación


- Genética Cuantitativa en Plantas de Fruto. - Alimentos Funcionales (contenido de fenoles en fruta) - Genómica Funcional y Biología Molecular en Plantas. - Fitopatología (Relación fruto - hongo patógeno)

Educación

  •  Plant Biology and Crop Production, UNIVERSITA DEGLI STUDI DI MILANO. Italia, 2010
  •  Ingeniería en Biotecnología Molecular, UNIVERSIDAD DE CHILE. Chile, 2006
  •  Licenciatura en Biotecnología Molecular, UNIVERSIDAD DE CHILE. Chile, 2003

Experiencia Académica

  •   Tesista Pregrado Other

    UNIVERSIDAD DE CHILE

    INTA

    Santiago, Chile

    2003 - 2004

  •   Estudiante de Doctorado Full Time

    UNIVERSITA DEGLI STUDI DI MILANO

    Facoltà di Scienze Agrarie

    Milán, Italia

    2007 - 2010

Experiencia Profesional

  •   Investigador Full Time

    BioSigma SA

    Colina, Chile

    2004 - 2007

Formación de Capital Humano


- April 2018 to date: undergraduate thesis of Diego Valderrama "QTL analysis for anthocyanin compound content in a plum F1 family". Course: Engineering in Molecular Biotechnology. Faculty of Sciences, University of Chile. Chile.

- April 2018 to date: undergraduate thesis of Wladimir Vega "Genetic analysis for low molecular weight phenol content in plum tissues". Course: Engineering in Biotechnology, Faculty of Natural Sciences, Mathematics and Environment, Metropolitan Technology University. Chile.

- April 2017 to date. Tutor Magister Thesis by Ailynne Sepúlveda González. Title of the Thesis Project: "IN VITRO ESTIMATION OF BIOACCESIBILITY OF COMPOUNDS WITH FUNCTIONAL POTENTIAL IN CHILEAN EXPORT FRUIT". Master in Nutrition and Food, Mention Healthy Food. INTA, University of Chile.

- November 2016 - January 2017. Tutor of the Research Unit of Ailynne Sepúlveda González. Title of the Report: "Development of spectrophotometric techniques for the detection of different families of phenols in Japanese plum fruits (Prunus salicina L.)". Master in Nutrition and Food, Mention Healthy Food. INTA, University of Chile.

- November 2016 - February 2017. Tutor of Student Investigation Unit Eduardo Pacheco. Title of the Report: "Bibliographic search and testing of microsatellite markers in commercial varieties of Japanese plum (Prunus salicina L.) Biotechnology Engineering Degree, Metropolitan Technological University (UTEM).

- April 2014 - Co-tutor of the first cycle degree memory (3 year career) of Michela Panzeri. Title of the report: "VALUTAZIONE DELLA SUSCETTIBILITÀ A Monilinia laxa IN SEMENZALI DA INCROCI CONTROLLATI DI PESCO, ATTRAVERSO METODI DI INOCULAZIONE ARTIFICIALE IN CONDIZIONI CONTROLLATE" (Evaluation of the susceptibility to Monilinia laxa in individuals from controlled crosses, through its reaction to artificial inoculum under controlled conditions). Production and plant protection career. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- November 2013 - Co-tutor of second cycle degree memory (5 year career) by Michele Pesenti. Title of the report: "RESISTENZA AL MARCIUME BRUNO IN FRUTTI DI PESCO: INDAGINI ANATOMICHE" (Resistance to brown rot in peach fruits: anatomical studies). Master's degree in Sciences of production and protection of vegetable crops. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- March 2013. Co-tutor of second cycle degree memory (5 year career) of Angelo Ciaciulli. Title of the report: "STUDI SUL CONTROLLO GENETICO DELLA RESISTENZA AL MARCIUME BRUNO (Monilinia laxa [Aderh. And Ruhl.] Honey) IN FRUTTI DI PESCO (Prunus persica [L.] Batsch)" (Studies on the genetic control of resistance to the brown rot in fruits of the peach tree). Career in Plant, Food and Agro-environmental Biotechnology. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- October 2013. Co-tutor of the first cycle degree memory (3 year career) of Agnese Manchia. Title of the report: "VALUTAZIONE DELLA SUSCETTIBILITA A MONILINIA LAXA IN PESCO CON UN NUOVO METODO DI FENOTIPIZZAZIONE" (Evaluation of the susceptibility to Monilinia laxa in peach with a new method of phenotyping). Career in Sciences and agronomic technologies. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- November 2012. Co-tutor of the first cycle degree memory (3-year career) of Marco Lasala. Title of the report: "VALUTAZIONE DELLO STADIO DI MATURAZIONE IN FRUTTI DI PESCO WITH CON UN NUOVO METODO NON DISTRUTTIVO" (Evaluation of the maturation stage in peach fruits with a non-destructive method). Career in Sciences and agronomic technologies. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- November 2012. Co-tutor of the first cycle degree memory (3 year career) of Chiara Perini. Title of the report: "VALUTAZIONE IN CAMPO DELLA SUSCETTIBILITA" A Monilinia spp. IN PESCO: MESSA TO PUNTO DI A METODO DI INOCULAZIONE ARTIFICIALE ". (Field evaluation of the susceptibility to Monilinia spp. In peach: development of a new method of artificial inoculation). Career in Sciences and agronomic technologies. Department of Agrarian and Environmental Sciences (DISAA), University of Milan
- January 2012. Co-tutor of the first cycle degree memory (3 year career) of Andrea Masini. Title of the report: "VALUTAZIONE DELLA MATURAZIONE ASSOCIATA ALLA SUSCETTIBILITA'AL MARCIUME BRUNO IN FRUTTI DI PESCO". (Evaluation of maturation associated with susceptibility to brown rot in peach fruits). Career in Sciences and agronomic technologies. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.

- July 2011. Co-tutor memory of the first cycle degree (3-year career) of Michele Pesenti. Title of the report: "VALUTAZIONE DELLA RESISTENZA AL MARCIUME BRUNO NEI FRUTTI DI PESCO". (Evaluation of the resistance to brown rot in peach fruits). Production and plant protection career. Department of Agrarian and Environmental Sciences (DISAA), University of Milan.


Difusión y Transferencia


PARTICIPATION IN SCIENTIFIC CONFERENCES:

• Genotyping by Sequencing and QTL Analysis for Phenolic Compound Content in Japanese Plum (Prunus salicina Lindl.). Pacheco I. International Plant and Animal Genome XXVI. January 13-17, 2018, San Diego, USA. ORAL PRESENTATION.

• Genetic and Polyphenol Profile Diversity in a Japanese Plum (Prunus salicina L.) Small Cultivate Collection: Towards Genetic Dissection of Functional Compound Content Trait. Pacheco I, Salazar J, Silva C, Sepulveda A, Shinya P, Morales H, Peña A, Infante R. International Plant and Animal Genome XXV. January 14 - 18, 2017, San Diego, USA. POSTER.

• "Polyphenol profiling in a Japanese plum (Prunus salicina L.) small cultivar collection: towards genetic dissection of functional compound content trait". Pacheco, I. (1), Salazar, J. (2), Silva, C., Shinya, P., Morales, H., Peña, A., Rodrigo Infante, R. XI Annual Meeting of the Chilean Association of Biology Vegetal, December 2016, Chillán, Chile. ORAR PRESENTATION

• GENETIC DISSECTION OF COMPLEX FRUIT QUANTITATIVE TRAITS IN PEACH PROGENIES. Igor Pacheco, Angelo Ciacciulli, Cassia Da Silva-Linge, Stefano Gattolin, Cinzia Colombi, Stefano Foschi, Martina Lama, Laura Rossini and Daniele Bassi. International Plant and Animal Genome XXIII. January 10 - 14, 2015, San Diego, USA. POSTER.

• ASSESSING BROWN ROT RESISTANCE IN PEACH FRUIT. Pacheco I., Quilot-Turion B, Bassi D. XIV EUCARPIA FRUIT BREEDING AND GENETICS SYMPOSIUM. Bologna 14-18 June 2015.
• GENETIC VARIABILITY AND POPULATION STRUCTURE OF PEACH ACCESSIONS FROM MAS.PES GERMPLASM BANK. Da C Silva Linge, G Chietera, I Pacheco, S Biffani, S Foschi, M Lama, L Rossini, D Bassi. In: VIII International Peach Symposium, Matera, Italy. June 17th-20th, 2013. POSTER.

• PEACH FRUIT QUALITY TRAITS INFLUENCE ON THE SUSCEPTIBILITY TO BROWN ROT (Monilinia laxa [Adehr. & Ruhl.] Honey): PRELIMINARY STUDIES IN A F1 PROGENY. I Pacheco, A Ciacciulli and D Bassi. In: VIII International Peach Symposium, Matera, Italy. June 17th-20th, 2013. POSTER.

• VALIDATION OF DAMETER DEVICE FOR PRECISE DETERMINATION OF HARVEST TIME ON MELTING-FLESH PEACHES. I Pacheco, M Lasala and D Bassi. In: In: VIII International Peach Symposium, Matera, Italy. June 17th-20th, 2013. POSTER.

• TOWARDS FASTER PHENOTYPING METHODS FOR BROWN ROT SUSCEPTIBILITY: USE OF ARTIFICIAL INOCULUM IN THE ORCHARD. I Pacheco, CM Perini, M Lama, S Foschi and D Bassi. In: In: VIII International Peach Symposium, Matera, Italy. June 17th-20th, 2013. ORAL COMMUNICATION.

• Genetic dissection of peach quality traits. R. Pirona, Pacheco I., Chietera G., Eduardo I., Da Silva C., Troggio M., Banchi e., Dondini L., Tartarini S., Pozzi C., Vecchietti A., Rossini L. - In : 6th International Rosaceae Genomics Conference, Trento, Italy. September 30th - October 4th, 2012. Oral presentation.

• ANALYSIS OF GENETIC DIVERSITY OF A PEACH (PRUNUS PERSICA) COLLECTION USING AFLP MARKERS. C. SILVA LINGE, I. PACHECO CRUZ, A. FRICANO, L. ROSSINI, D. BASSI - In: Proceedings of the 54th Italian Society of Agricultural Genetics Annual Congress [sl]: Società Italiana di Genetica Agraria, 2010 Sep. - ISBN 9788890457005. - pp. Poster Communication Abstract - 1.52.

• Peach volatile compounds QTL analysis and candidate gene co-localization. G. Chietera, I. Eduardo, I. Pacheco, R. Pirona, D. Bassi, A. Spinardi, L. Rossini, C. Pozzi, A. Vecchietti - In: Proceedings of the 54th Italian Society of Agricultural Genetics Annual Congress [ sl]: Società Italiana di Genetica Agraria, 2010 Sep. - ISBN 9788890457005. - pp. poster 7.22.

• PEACH VOLATILE COMPOUNDS QTL AND CANDIDATE GENE COLOCATION. Eduardo, I., Chietera, G., Pacheco, I., Bassi, D., Pirona, R., Rossini, L., Vecchietti, A. and Pozzi, C. - In: 5th International Rosaceae Genomics Conference, Cape Town , South Africa November 14-17, 2010. Poster No. 13.

• RESEQUENCING OF A PEACH GENOTYPE AND SNP DEVELOPMENT FOR MAPPING AND GENETIC DIVERSITY ANALYSIS. Tartarini, S., Dondini, L., Parla, R., Venturi, S., Sansavini, S., Marasco, E., Mantovani, V., Pirona, R., Rossini, L., Cattonaro, F., Pacheco Cruz, I. Bassi, D. and Verde, I. - In: 5th International Rosaceae Genomics Conference, Cape Town, South Africa. November 14-17, 2010. Poster No. 45.

• "Gene Expression And QTL Mapping Of Aroma And Brown Rot Disease In Peach". Vecchietti, A., Eduardo, I., Pirona, R., Pacheco, I., Chietera, G., Bassi, D., Rossini, L. Plant & Animal Genomes XVII Conference. San Diego, CA. January 2009

• "QTL mapping of fruit quality traits and volatile compounds in peach and candidate gene collocation". Eduardo, I., Pacheco, I., Chietera, G., Bassi, D., Rossini, L., Vecchietti, A. VII International Peach Symposium. Lleyda, Spain. June 2009

• "QTL Mapping for Peach (Prunus persica L. Batsch) Resistance to Powdery Mildew and Brown Rot". Pacheco, I., Eduardo, I., Rossini, L., Vecchietti, A., Bassi, D. LIII Annual Congress of the Italian Society of Agricultural Genetics (SIGA). Turin, Italy. September 2009.

• “Monitoring of bio-mining bacterial community present in mineral ore samples through quantitative real-time PCR”. Pacheco, I., Ehrenfeld, N., Aravena, A., Maass, A., Parada, P.and Badilla, R. XXVIII Latin-American Microbiology congress. Pucón Chile. October of 2006.

• “Optimisation of macro-arrays: Examining the performance of nylon membrane hybridations”. González-Agüero, M., Pacheco, I., González M., and Cambiazo, V. XXI Congress of the Chilean Society of Cellular Biology, Pucón, Chile. October of 2003.

OUTREACH AND EXTENSION:
• Talk "Genetics of 'difficult' characters applied to the varietal development in the genus Prunus." Dictated in the Center for Advanced Studies in Fruit Production (CEAF, VI Region, Chile) on April 4, 2017, the main advances in the characterization of complex phenotypes, of interest for Chilean fruit growing, were presented to the researchers and students of the center, such as the resistance to monilysis and the content of phenolic substances in stone fruits.

• Talk "Enhancement of research and teaching in genetics and nutrition: study of agrifood varieties for their improvement in terms of nutraceutical value", in the context of the weekly Scientific Meetings of INTA. Dictated on May 3, 2017 at the INTA. The advances in research of the insertion project in the academy were presented.

• Talk "Plant genetic improvement and nutrition: towards an increase in the functional capacities of foods of vegetable origin". In the context of the academic day "Functional Genomics: the Challenge of Understanding Biological Systems". Dictated on September 9, 2016 at the Institute of Entomology of the Metropolitan University of Education Sciences (UMCE).

• Article "Genetic Improvement: how can it improve our health?" Indualimentos Magazine. April 2016, pp 10-14.
• Interview "Transgenic and genetically improved foods" in Radio La Cooperativa's "La Justa Medida" Program. Broadcast on December 6, 2015. Podcast available at http://programas.cooperativa.cl/justamedida/

• October 2015 - Scientific Fair, Nobel Prize School of Parral. Plenary conference "Genetics in your home", dictated for third and fourth year high school students of 5 high schools of Parral, Región del Maule, Chile.

• Article "Nutri-breeding, development of plant varieties with greater nutraceutical value for the Chilean market". Nutrition and Life Magazine, May - June 2015 edition, pp. 26-31.

• Scientifi Meeting "Strumenti Genomici per il Miglioramento di Piante da Frutto: Sequenziamento del Genoma and Applicazioni di Selezione Assistita all'uso dei Marcatori". Held at the Congress Center Cesena Fiera on May 4, 2012. Organized by the CRPV and the Regional Government of Emilia-Romagna. Title of the Conference: "Resistenza al marciume bruno e all'oidio in pesco: primi strumenti per la selezione assistita". Outreach activity addressed to the community of fruit growers in northern Italy and to private financing entities (banking foundations of the Emilia-Romagna region).

• Seminar of diffusion "Genome-assisted breeding for peach [Prunus persica (L) Batsch] fruit quality and disease resistance: new traits and phenotyping challenges". Held at the CEAF-INIA Rayentué on August 26, 2011, in the context of the center's periodic seminars, and organized by Dr. Manuel Pinto. Directed to researchers of the center and fruit producers in the area.

• Assistant instructor in the summer course "Molecular Biology and Biotechnology for Middle School students". January 2003. Faculty of Sciences, University of Chile, CBB Instituto Milenio and Explora-CONICYT. Initiative coordinated by Professors Ariel Orellana and Lee Meisel.


Premios y Distinciones

  •   Mejor Poster

    PARCO TECNOLOGICO PADANO - LODI

    Italia, 2008

    Premio al mejor póster en el workshop “Metodologie di genotipizzazione e diagnostica molecolare per l’agroalimentare”. Parco Tecnologico Padano, Lodi, Italia. 22 de Octubre de 2008. Título del póster: “SSR genotyping in Peach (Prunus persica (L.) Batsch) using Multiplex-ready strategy”.

  •   Doctoral Fellowship

    MIUR: Ministry of Education, University and Research

    Italia, 2007

    Fellowship for the development of doctoral thesis: Brown rot resistance in "Prunus persica" fruits, a genomics approach.

  •   Doctoral Fellowship "Gestión Propia"

    CONSEJO NACIONAL DE CIENCIA Y TECNOLOGIA

    Chile, 2008

    Fellowships to Chileans and / or foreigners with permanent residence in Chile, to initiate or continue studies leading to the attainment of the academic degree of Doctor, in academic and / or research institutions of excellence in foreign countries, so that return apply their knowledge to contribute to the scientific, academic, economic and social development of the country.

  •   Post-doctoral fellowship “Beca Chile de Post-Doctorado en el Extranjero”,

    CONSEJO NACIONAL DE CIENCIA Y TECNOLOGIA

    Chile, 2013

    Promote the productivity and future scientific positioning of researchers with the academic degree of Doctor, in the academic and productive field, through their incorporation into prestigious and consolidated research groups abroad, in such a way that, upon their return to the country contribute to the consolidation of national universities and scientific centers.


 

Article (15)

Development and applicability of GBS approach for genomic studies in Japanese plum (Prunus salicina Lindl.)
Deletion of the miR172 target site in a TOE-type gene is a strong candidate variant for dominant double-flower trait in Rosaceae
Linkage and association mapping for the slow softening (SwS) trait in peach (P. persica L. Batsch) fruit
Genome-enabled predictions for fruit weight and quality from repeated records in European peach progenies
Genotyping by Sequencing for SNP-Based Linkage Analysis and Identification of QTLs Linked to Fruit Quality Traits in Japanese Plum (Prunus salicina Lindl.)
Preliminary results on effectiveness of marker-assisted seedling selection applied to Mendelian traits in peach
Identifying SNP markers tightly associated with six major genes in peach [Prunus persica (L.) Batsch] using a high-density SNP array with an objective of marker-assisted selection (MAS)
Genetic dissection of fruit weight and size in an F-2 peach (Prunus persica (L.) Batsch) progeny
Whole-genome analysis of diversity and SNP-major gene association in peach germplasm
A Unique Mutation in a MYB Gene Cosegregates with the Nectarine Phenotype in Peach
QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny
Fine mapping and identification of a candidate gene for a major locus controlling maturity date in peach
Genetic dissection of aroma volatile compounds from the essential oil of peach fruit: QTL analysis and identification of candidate genes using dense SNP maps
QTL analysis of fruit quality traits in two peach intraspecific populations and importance of maturity date pleiotropic effect
Identification of woolliness response genes in peach fruit after post-harvest treatments

ConferencePaper (7)

Fruit breeding for enhanced healthy-food properties: plums as a model to study genetics and genomics of phenolic compounds
Functional Genomics As a Tool to Unveil Bioactive Compounds Accumulation in Commercial and Native Plants from Chile
Genomics of Fruit Quality, Nutritional Composition and Phytochemical Content of Cherries and Plums
Genotyping by Sequencing and QTL Analysis for Phenolic Compound Content in Japanese Plum (Prunus salicina Lindl.)
Diversity of phenolic compound behavior after harvest in Japanese plum: towards breeding fruits with enhanced functional food quality
Exploring and exploiting phenotypic and genetic diversity in peach: Identification of major genes and QTLs by GWAS
Polyphenol profiling in a Japanese plum (Prunus salicina L.) small cultivar collection: towards genetic dissection of functional compound content trait

ConferencePoster (7)

GENETIC ANALYSES FOR ANTHOCYANIN CONTENT IN A JAPANESE PLUM F1 PROGENY
Genetic and Polyphenol Profile Diversity in a Japanese Plum (Prunus salicina L.) Small Cultivate Collection: Towards Genetic Dissection of Functional Compound Content Trait.
POSTHARVEST MONITORING IN Prunus salicina FOR MARKER TRAIT ASSOCIATIONS
RNA-SEQ ANALYSIS OF POSTHARVEST BEHAVIOR IN KIWIFRUIT Actinidia deliciosa AFTER 1-MCP AND ETHREL APPLICATIONS
SNP AND INDEL VARIANT CALLING USING GBS AND WHOLE GENOME SEQUENCING IN PARENTS AND F1 POPULATION FROM A JAPANESE PLUM (Prunus salicina L.) BIPARENTAL CROSS.
QTL IDENTIFICATION FOR AGRONOMIC TRAITS IN PRUNUS SALICINA THROUGH GENOTYPE BY SEQUENCING (GBS)
GENETIC DISSECTION OF COMPLEX FRUIT QUANTITATIVE TRAITS IN PEACH PROGENIES

Proyecto (5)

"Sweet Pekeetah": A technological-commercial model for a new Chilean variety of fruit
Development of a core facility for food analysis based on the incorporation of physiological phenomena, such as digestion and fermentation, with TWINSHIME technology
Enhancement of research and teaching in genetics and nutrition: study of agri-food varieties for their improvement in terms of nutraceutical value
Genetic studies for the improvement of stone fruit nutraceutical quality
Packaging and start-up of Redoxcell, a service to evaluate the antioxidant impact of food matrices in living cells

Article (3)

Assessing genetic diversity in peach by AFLP and SSR markers
Susceptibility to Brown Rot (Monilinia spp.) in Peach (Prunus persica L. Batsch): influence of the Maturity Date in an F1 Progeny
TOWARDS FASTER PHENOTYPING METHODS FOR BROWN ROT SUSCEPTIBILITY: USE OF ARTIFICIAL INOCULUM IN THE ORCHARD

Patent (1)

Method of identification and quantification of microorganisms useful in the process of bio-leaching

Review (1)

Brown Rot Strikes Prunus Fruit: An Ancient Fight Almost Always Lost
36
Igor Pacheco

Profesor Asistente

Laboratorio de Bioinformática y Expresión Génica

INTA - Universidad de Chile

Santiago, Chile

2
RODRIGO INFANTE

Profesor Titular

Producción Agrícola

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Lee Meisel

Associate Professor

Institute of Nutrition and Food Technology (INTA), University of Chile

Macul, Chile

1
JUAN SALAZAR

Investigador

Producción Agrícola

UNIVERSIDAD DE CHILE

Santiago de Chile, Chile

1
Veronica Cambiazo

Associate Professor

Unidad de Nutrición Básica

UNIVERSIDAD DE CHILE - INTA

Santiago, Chile

1
Ariel Orellana

Profesor Titular

Centro de Biotecnologia Vegetal

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Mauricio Gonzalez

Profesor Titular

INTA

UNIVERSIDAD DE CHILE - INSTITUTO DE NUTRICION Y TECNOLOGIA DE ALIMENTOS

Santiago, Chile

1
Leonardo Pavez

Profesor Asociado

Instituto de Ciencias Naturales

Universidad de Las Américas

Santiago, Chile