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PAMELA ALEJANDRA LEAL ROJAS

Profesor Asociado

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

Líneas de Investigación


1) Caracterización molecular de tumores tracto biliar. 2) Estudio de vesículas extracelulares como fuente de marcadores moleculares. 3) Evaluación de extractos naturales en modelos preclínicos.

Educación

  •  Biología Celular y Molecular Aplicada, UNIVERSIDAD DE LA FRONTERA. Chile, 2005
  •  Ingeniero Agrónomo, UNIVERSIDAD DE LA FRONTERA. Chile, 2001
  •  Posdoctorado, JOHNS HOPKINS UNIVERSITY. Estados Unidos, 2015

Experiencia Académica

  •   PROFESOR ASOCIADO Full Time

    UNIVERSIDAD DE LA FRONTERA

    Facultad de Ciencias Agropecuarias y Medioambiente

    Temuco, Chile

    2006 - A la fecha

Experiencia Profesional

  •   Profesora Asociada Full Time

    Universidad de La Frontera

    Temuco, Chile

    2006 - A la fecha

  •   Directora de Cooperación Internacional Part Time

    Universidad de La Frontera

    Temuco, Chile

    2017 - 2019

  •   Directora de Calidad Part Time

    Universidad de La Frontera

    Temuco, Chile

    2020 - A la fecha

Formación de Capital Humano


2019
Joaquín Sepúlveda Hafemann. Efecto del inhibidor Surmanina en lineas celulares de cáncer de vesícula Biliar. Trabajo para optar al título de Bioquímico. Universidad de La Frontera. Temuco-Chile.
Profesor Guía. Terminada

2019
Ramón Silva Pezoa. Validación de potenciales blancos terapéuticos en cáncer avanzado de vesícula biliar identificados mediante proteómica cuantitativa. Tesis Doctoral. Programa de
Doctorado en Ciencias mención Biología Celular y Molecular Aplicada. Universidad de La Frontera. Terminada
Profesor Tutor.

2020
Luis Vergara Gómez. Caracterización del perfil proteómico de organoides derivados de pacientes con cáncer de vesícula biliar y su asociación con respuesta a gemcitabina
Tesis Doctoral. Programa de Doctorado en Ciencias mención Biología Celular y Molecular Aplicada. Universidad de La Frontera.
Profesor Tutor. En proceso


Difusión y Transferencia


2007
Gidekel M, Gutiérrez A, Destefano-Beltrán L, Leal P, Dinamarca J, Guerra E. Low temperature responsive nucleotide sequences and used thereof (2007). USPTO Application #: 20070118922.

2005
Gidekel M, Gutiérrez A, Leal P, Destefano-Beltrán L, Dinamarca J, Guerra E. Novel Plant promoter transgenic plants expressing a Deschampsia antarctica modified gene transcript and
uses thereof (2005). Patent N° US7273931B2.


Premios y Distinciones

  •   Artículo más citado

    UNIVERSIDAD DE LA FRONTERA

    Chile, 2021

    McCall MN, Kim MS, Adil M, Patil AH, Lu Y, Mitchell CJ, Leal-Rojas P, Xu J, Kumar M, Dawson VL, Dawson TM, Baras AS, Rosenberg AZ, Arking DE, Burns KH, Pandey A, Halushka MK. Toward the human cellular microRNAome. Genome Res. 2017 Oct;27(10):1769-1781. doi: 10.1101/gr.222067.117. Epub 2017 Sep 6.

  •   Artículo más citado

    UNIVERSIDAD DE LA FRONTERA

    Chile, 2016

    Min-Sik Kim, Sneha M. Pinto, Derese Getnet, Raja Sekhar Nirujogi, Srikanth S. Manda, Raghothama Chaerkady, Anil K. Madugundu, Dhanashree S. Kelkar, Ruth Isserlin, Shobhit Jain, Joji K. Thomas, Babylakshmi Muthusamy, Pamela Leal-Rojas, Praveen Kumar, Nandini A. Sahasrabuddhe et al. A draft of the human proteome. Nature, May 2014, 509(7509): 575–581. (FI: 42.351)


 

Article (42)

Controlled Release of Caffeic Acid and Pinocembrin by Use of nPSi-beta CD Composites Improves Their Antiangiogenic Activity
Exosomes and cystic Echinococcosis. Systematic review
CRISPR/Cas13-Based Platforms for a Potential Next-Generation Diagnosis of Colorectal Cancer through Exosomes Micro-RNA Detection: A Review
Anthocyanin-Rich Extracts of Calafate (Berberis microphylla G. Forst.) Fruits Decrease In Vitro Viability and Migration of Human Gastric and Gallbladder Cancer Cell Lines
Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
Hippo-YAP1 Is a Prognosis Marker and Potentially Targetable Pathway in Advanced Gallbladder Cancer
The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties
Mucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma
PIM1 kinase promotes gallbladder cancer cell proliferation via inhibition of proline-rich Akt substrate of 40 kDa (PRAS40).
Effects of clopidogrel on inflammatory cytokines and adhesion molecules in human endothelial cells=> Role of nitric oxide mediating pleiotropic effects.
Small molecule inhibitor screening identifified HSP90 inhibitor 17-AAG as potential therapeutic agent for gallbladder cancer
The ERK/MAPK pathway is overexpressed and activated in gallbladder cancer.
The non-receptor tyrosine kinase TNK2/ACK1 is a novel therapeutic target in triple negative breast cancer
Toward the human cellular microRNAome
miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in gastric cancer through regulation of the PI3K/AKT/mTOR pathway
The Gene Expression Status of the PI3K/AKT/mTOR Pathway in Gastric Cancer Tissues and Cell Lines
Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
Global phosphotyrosine survey in triple-negative breast cancer reveals activation of multiple tyrosine kinase signaling pathways
Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer
Quantitative phosphoproteomic analysis of IL-33-mediated signaling
Rapamycin and WYE-354 suppress human gallbladder cancer xenografts in mice
A draft map of the human proteome.
Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
Genome-wide methylation profiling reveals Zinc finger protein 516 (ZNF516) and FK-506binding protein 6 (FKBP6) promoters frequently methylated in cervical neoplasia, associated with HPV status and ethnicity in a Chilean population
Identification of Prosaposin and transgelin as potential biomarkers for gallbladder cancer using quantitative proteomics
Immunohistochemical expression of vascular endothelial growth factor A in advanced gallbladder carcinoma.
The PI3K/AKT/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance.
AKT/mTOR substrate P70S6K is frequently phosphorylated in gallbladder cancer tissue and cell lines
Cellular FLICE-like Inhibitory Protein Long Form (c-FLIPL) Overexpression is Related to Cervical Cancer Progression
Connective Tissue Growth Factor Immunohistochemical Expression Is Associated With Gallbladder Cancer Progression
Immunohistochemical Expression of Phospho-mTOR Is Associated With Poor Prognosis in Patients With Gallbladder Adenocarcinoma
In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells
Inhibition of connective tissue growth factor (CTGF/CCN2) in gallbladder cancer cells leads to decreased growth in vitro
Clinical and public health research using methylated DNA Immunoprecipitation (MeDIP): A comparison of commercially available kits to examine differential DNA methylation across the genome
Genotyping of Human Papillomavirus in Cervical Intraepithelial Neoplasia in a High-Risk Population
Pericytes: New Approaches in Regenerative Therapy, Cerebrovascular Pathology and Cancer
[Promoter hypermethylation gene patterns in gynecological tumors].
Detection and genotyping of human papillomavirus in biopsies of uterine cervical adenocarcinoma
Melanoma Hyperpigmentation Is Strongly Associated With KIT Alterations
The Axl receptor tyrosine kinase confers an adverse prognostic influence in pancreatic cancer and represents a new therapeutic target
Mesothelin is a specific biomarker of invasive cancer in the Barrett-associated adenocarcinoma progression model: translational implications for diagnosis and therapy
Identification and characterization of three novel cold acclimation-responsive genes from the extremophile hair grass Deschampsia antarctica Desv.

Abstract (5)

HSP90 Expression and Antitumoral Activity of Geldanamicin and Its Analog in Gallbladder Carcinoma
mTOR/P70S6K signaling pathway as a potential target for advanced gallbladder cancer therapy
Expression Analysis of AKT/mTOR Signaling Pathway in Primary Tumors and Cell Lines Derived from Gallbladder Cancer
Level of miR-125B and miR-451 and Deregulated Expression of PI3K/AKT/mTOR Pathway in Gastric Cancer
Heat Shock Protein (HSP)-90 Is Overexpressed in Gallbladder Carcinoma (GBC)

Proyecto (17)

Accessing untapped sources of natural products: An innovative transdisciplinary multi-omics approach for bioactive metabolites discovery in Actinobacteria and Cyanobacteria from extreme environments
Connecting molecular subtypes of colorectal cancer with extracellular vesicles heterogeneity in patient-derived organoids
Setting up de una plataforma de producción de anticuerpos monoclonales recombinantes, utilizando el biomarcador CDLN18 de cáncer de vesícula biliar
Circulating exoDNA as a next generation biospecimen for liquid biopsy of pancreatic-biliary cancers
CARACTERIZACIÓN FENÓLICA DE EXTRACTOS DE FRUTOS DE CALAFATE Y SU POTENCIAL USO COMO AGENTE HIPOGLICEMIANTE y ANTICANCERíGENO
Efecto del clopidogrel sobre mediadores de inflamación y adhesión celular en células endoteliales humanas
Identificación de componentes diferenciales del microbioma intestinal entre individuos hipercolesterolémicos y controles.
Proteomic signature of gallbladder cancer secreted exosomes=> a dynamic approach to delineate their potential role during malignant progression and their clinical relevance as novel reservoir of circulating biomarkers
Identification and validation of early diagnostic gallbladder cancer biomarkers in Chilean patients by quantitative proteomics. A phase 1 and 2 Biomarker validation trial.
Using a quantitative proteomic as a useful tool to identify potencial biomarkers in gallbladder cancer.
Improving the production of phenylpropanoids with antioxidant activity in Highbush blueberry (Vaccinium corymbosum L.) using Methyl Jasmonate and UV-­B radiation and its effect on gastric cancer chemoprevention.
Rol de la activación de la vía AKT/mTOR en la patogénesis y progresión del cáncer de vesícula biliar=> estudio inmunohistoquímico en matrices de tejido (TMAs).
Nanopartículas modificadas con cobre en el tratamiento del cáncer=> Evaluación in vitro, toxicología y biocompatibilidad.
Validation of MTOR metabolic pathway inactivation for gallbladder cancer targeted therapy.
Rol de las proteínas SKP2 y FBW7, componentes del complejo SCF E3 ligasa, en cáncer de la vesícula biliar.
Centro de Excelencia en Biociencias de la Salud. PBCT–World Bank.
Deschampsia antarctica DESV.=> Prospección de genes y agentes fotoprotectores ultravioleta para su uso en la industria farmacéutica

Review (1)

Targeting specific molecular pathways holds promise for advanced gallbladder cancer therapy
39
PAMELA LEAL

Profesor Asociado

Departamento de Ciencias Agronómicas y Recursos Naturales

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

25
Juan Roa

Chair

Pathology

Pontificia Universidad Catolica de Chile

santiago, Chile

14
Patricia García

Profesor Asistente

ANATOMIA PATOLOGICA

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

10
Helga Weber

Investigador

Vicerrectoría de Investigación y Post Grado

Universidad de la Frontera

Temuco, Chile

8
Priscilla Brebi

Profesor Asociado

Departamento de Anatomía Patológica.

Universidad de La Frontera

Temuco, Chile

7
Carmen Ili

Academico Asociado

Scientific and technological Bioresource Nucleus (BIOREN)

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

7
Carolina Bizama

Assistant professor

Department of Pathology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

7
Ismael Riquelme

Académico - Investigador

Ciencias de la Salud

UNIVERSIDAD AUTÓNOMA DE CHILE

Temuco, Chile

4
Jaime Lopez

Asistente de Investigación

Anatomía Patologica

Universidad de La Frontera

Temuco, Chile

4
Pablo Letelier

Docente Planta Permanente (Instructor)

UNIVERSIDAD CATOLICA DE TEMUCO

temuco, Chile

4
Bruno Nervi

Associated Professor

Hematology - Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

3
Juan Araya

Pathologist

Anatomía Patológica

Hospital Dr. Hernan Henríquez Aravena

Temuco, Chile

3
Lorena Rosa

PhD

Departamento de Patología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Luis Salazar

Profesor Titular

Ciencias Básicas

Universidad de La Frontera

Temuco, Chile

2
Pablo Guzmán

Pathologist

Hospital Regional Dr. Hernán Henríquez Araven

Temuco, Chile

2
Kurt Buchegger

Investigador Postdoctoral

Ciencias Básicas

Universidad de La Frontera

Temuco, Chile

2
Sergio Vargas

Investigador

Hematología-Oncología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Fernando Romero

Profesor JC, asociado

Ciencias Preclínicas

Universidad de La Frontera

Temuco, Chile

1
Alexis Kalergis

Full Professor

Microbiología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE. FACULTAD DE CIENCIAS BIOLÓGICAS

Santiago, Chile

1
Manuel Gidekel

GERENTE OPERACIONES Y PRESIDENTE

CONSORCIO DE TECNOLOGIA E INNOVACION PARA LA SALUD

SANTIAGO, Chile

1
Enrique Montiel

Profesor Asistente

Ciencias Básicas

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

1
jose valbuena

Associate Professor

Anatomic Pathology

PONTIFICIA UNIVERSIDAD CATOLICA. FACULTAD DE MEDICINA/ESCUELA DE MEDICINA/ DEPARTAMENTO DE ANATOMÍA PATOLÓGICA

santiago, Chile

1
Jennie Risopatrón

Académico y Director del CEBIOR

Ciencias Básicas

Universidad de La Frontera

Temuco, Chile

1
Claudio Toro

EXECUTIVE DIRECTOR

CENTRO DE INVESTIGACIÓN DE POLÍMEROS AVANZADOS

CONCEPCIÓN, Chile

1
Leon Bravo

Profesor Titular

Ciencias Agronómicas y Recursos Naturales

UNIVERSIDAD DE LA FRONTERA

Temuco, Chile

1
Sergio Munoz

Profesor Titular

Salud Publica

Universidad de La Frontera

Temuco, Chile

1
Juan Calderon

Investigador

Facultad de Medicina

Universidad del Desarrollo

Santiago, Chile

1
Paulina Araya

COORDINADORA ACADEMICA

Facultad de Medicina

Universidad del Desarrollo

SANTIAGO, Chile

1
Javiera Obreque

Investigadora Postdoctoral

CECAN - PUC

Santiago, Chile

1
Lida Fuentes

Researcher

Centro Regional de Estudios en Alimentos Saludables

Valparaiso, Chile

1
Marely Cuba

Titular professor

Ciencia y Tecnología Vegetal

UNIVERSIDAD DE CONCEPCIÓN, CAMPUS LOS ÁNGELES

Los Ángeles, Chile

1
Dina Guzman

Profesor Asistente

Universidad San Sebastián

Concepción , Chile