Neuropharmacology; Neurochemistry; Neuroplasticity; PathoPhysiology of Perinatal Asphyxia; Neuropharmacology of basal ganglia; Neurognesis postnatal; Plasticidad morfofuncional del sistema nervioso frente a injurias cerebrales; Muerte celular/Neuroprotección

Postdoctoral Student
Nancy Farfán Troncoso Mesenchymal stem cells-secretome to the rescue of mitochondrial integrity in perinatal asphyxia via NRF2. Fondecyt 3210771

Supervision de Tesis de Doctorado

Marta Zamorano Cataldo, PhD in Biochemistry, Faculty of Chemistry and Pharmacy, Universidad de Chile.Effect of Intranasal DFX-preconditioned Mesenchymal Stem Cell Secretome administration on perinatal asphyxia induced mitochondrial dysfunction and cell loss in rat hippocampus: Role of Nrf2 activation. In Progress (Supervisor)

Andrea Tapia Bustos, PhD in Pharmacology, Faculty of Chemistry and Pharmacy, Universidad de Chile. Perinatal asphyxia produces long-term oligodendrocyte damage and demyelination in rat brain: prevention by intranasal administration of stem cells. Co supervisor, Finished July 16 Julio 2020. (Supervisor)

Ronald Perez Lobos , PhD in Biochemistry, Faculty of Chemistry and Pharmacy, Universidad de Chile. The vulnerability of basal ganglia is due to a long-term impairment of mitochondrial dynamics: role of HIF-1alpha and PARP-1 signalling. In progress

Manuel Gutierrez Hernández , PhD in Biomedical Sciences (DCBM). Faculty of Medicine, University of Chile. RGS proteins associated to dopamine receptors: Modulation by unilateral 6-OHDA lesion and dopamine agonists in the basal ganglia of the rat. Finished August 2016 (Co-Supervisor)

Tanya Neira, Doctorate in Biomedical Sciences (DCBM). Faculty of Medicine, University of Chile. PARP-1 and inflammatory responses in mesencephalon of rat: Effects of perinatal asphyxia. Finished January 2015 (Co-Supervisor)

Edgardo Rojas Mancilla, Doctorate in Biomedical Sciences (DCBM). Faculty of Medicine, University of Chile. Severe perinatal asphyxia produces HIF-1? translocation and astrocyte reactivity: Morphological and functional changes in rat hippocampus. Finished July 31,2015(Co-supervisor)

Supervisión of Master thesis

Sofía Constanza Adasme Reyes, Master in Biochemistry, Faculty of Chemistry and Pharmacy, Universidad de Chile, Alda-1 administration decreases oxidative stress and brain neuroinflammation associated with chronic alcohol and nicotine consumption in UChB rats. In Progress 2021 (Co-supervisor)

Raúl Alvarado Rosas, Master in Neuroscience, Faculty of Medicine, University of Chile. The N-acetylcysteine-induced reduction of chronic alcohol
comsumption is associated to the activation of the Nrf2/ARE pathway in high-alcohol-drinking UChB rats. Finished March 22, 2021 (Co-supervisor)

Norton Contreras Paredes. Master in Neuroscience, Faculty of Medicine, University of Chile. Effect of treatment with mesenchymal stem cell-derived secretomes on cognitive deficits induced by a perinatal asphyxia model. In Progress (Co-Supervisor)

Martina Redel Master in Neurosciences, Faculty of Medicine, University of Chile. Effect of the administration of mesenchymal stem cell-derived secretomes on oligodendrocyte development in a perinatal asphyxia model. In Progress (Supervisor)

Jessica Sánchez Torres, Master in Neurosciences, Faculty of Medicine, University of Chile. Effect of the histamine H3 receptor antagonist on spatial memory, neurogenesis and hippocampal plasticity in rats with perinatal asphyxia. Finished March 19, 2018 (Co-Supervisor).

Javier Ignacio Silva García Master in Neuroscience, Faculty of Medicine, University of Chile. Effect of voluntary physical exercise on memory capacities in an animal model of perinatal asphyxia. Completed June 6, 2017 (Co-supervisor)

Marianne Alexandra Buscaglia Fernández Master in Biochemistry, Faculty of Medicine, University of Chile. Effect of acute administration of ethanol in the formation of protein-acetaldehydeadducts in the mesocorticolimbic dopaminergic system. Finished January 31, 2013 (Co-Supervisor).

Daniela Esmar Gutierrez Master in Neuroscience, Faculty of Medicine, University of Chile. Postnatal cell proliferation in the hippocampus is modulated by dopaminergic systems in the rat. Finished, December 6, 2013 Supervisor.

María José Díaz Latas, Master in Biochemistry, Andres Bello University. Quantitative Analysis of Copper in rat hippocampus. Finished May 2012 (Co-Supervisor)

María Mercedes Casas Alarcón, Master in Physiopathology, Medical Faculty, University of Chile. Involvement of ryanodine receptor-2 in memory associated with fear. Finished December 2011 (Co-Supervisor)

Verena Klawitter Melkonian, Master in Neuroscience, Medical Faculty, University of Chile (2004-2006). Effects of perinatal on basal ganglia neurocircuitry. Approved with honour on May 30, 2006 (selected among the 3 best thesis by the Chilean Society of Molecular and Cellular Biology, 2006) (Co-Supervision).

Undergraduate Student Supervisor

María José Ortega, Javiera Rojas Ríos, Effects of intranasal administration of secretomes derived from pre-conditioned stem cells on demyelination induced by perinatal asphyxia. TThesis to qualify for the degree of Medical Technologist. University Andrés Bello. Finished September 2020.

Araya M, Monzon E, Mesenchymal stem cell secretomas as treatment in perinatal asphyxia Thesis to qualify for the degree of Medical Technologist. Andres Bello University. Finished August 2019

Jorge Ruiz Campillay Study of mitochondrial function and neuronal vulnerability to recurrent metabolic insults in brain tissue exposed to perinatal asphyxia. Thesis to qualify for the degree of Medical Technologist, University of Chile Finished 2016 (Supervisor).

Mariana Rubio Effect of deafferentation of dopaminergic afferents on the expression of dopamine receptors, RGSs proteins and their relationship with the neurogenesis of the hippocampus of adult rats. Thesis to qualify for the degree of Medical Technologist, Universidad San Sebastian. Finished 2015 (Supervisor)

Marcos Sert Dependent vulnerability of perinatal asphyxia of dopaminergic neurons to postnatal metabolic insults ”Montanarer Perez Sofia, Effect of nperinatal asphyxia and dopaminergic de-afferentiation on the expression of SNGN in rat brain evaluated with immunohistochemistry, confocal microscopy and stereological quantification. TThesis to qualify for the degree of Medical Technologist. Andres Bello University, Completed December 2015

Paloma Saez Study of SOD2 expression in dopaminergic neurons of rat brains subjected to perinatal asphyxia. TThesis to qualify for the degree of Medical Technologist, Andres Bello University, Finished December 2015 (Supervisor).

Sofia Montanares Perez Effect of nperinatal asphyxia and dopaminergic de-afferentiation on the expression of SCGN in rat brain evaluated with immunohistochemistry, confocal microscopy and stereological quantification. Thesis to qualify for the degree of Medical Technologist, Andres Bello University, Finished December 2015 (Supervisor)

Benjamín Rivera, Characterization of RGSs proteins and dopamine receptors in the sub-ventricular zone (SVZ) in a rat perinatal asphyxia model. Thesis to qualify for the degree of Medical Technologist, University of Chile. Finished 2014 (Supervisor)

Belén Urqueta Herrera, Inflammation and microglial activation: Effects of perinatal asphyxia in neonatal rat hippocampus. Thesis to qualify for the degree of Medical Technologist. University of Chile. Finished 2013 (Supervisor).

Antonella Jara, Regulation of postnatal neurogenesis by the basic fibroblast growth factor, bFGF, in normal and brain injury conditions: role of the Spry, SEF and FLRT3 proteins. Thesis to qualify for the degree of Biochemistry, Catholic University of Valparaíso. Finished Dec 2010 (Supervisor)

LUN (Diario Las Ultimas Noticias) Entrevista: Biofármaco previene el daño causado por la falta de oxigeno en recién nacidos. Octubre 21, 2020.

Entrevista radial Radio DUNA: Paola Morales y biofármaco para tratar asfixia en recién nacidos: “Hasta el momento hemos encontrado buenos resultados con una sola administración. Octubre 27, 2020 https://unlimited1-cl-vod.dps.live/vod/cl2/duna/618396/czoRpDX1u3o.mp3?nimblesessionid=39914391
https://www.duna.cl/programa/aire-fresco/2020/10/27/paola-morales-y-biofarmaco-para-tratar-asfixia-en-recien-nacidos-hasta-el-momento-hemos-encontrado-buenos-resultados-con-una-sola-administracion/

Conferencia ICBM-Depto Neurociencias, Facultad de Medicina, Universidad de Chile: Secretomas derivados de células madres mesenquimales: Una alternativa en el tratamiento de alteraciones metabólicas
al momento de nacer. Octubre 29, 2020 https://www.youtube.com/watch?v=TGoZjbpDGVY

Conferencia Programa Fisiopatología Facultad de Medicina campus oriente: “Mesenchymal stem cells as a
novel therapeutic strategy against neonatal asphyxia”. Septiembre 2, 2019.

Nota de prensa La tercera, Qué pasa: Científicos chilenos crean innovador spray nasal basado en células madre para revertir daños cerebrales. Abril, 23, 2019 https://www.latercera.com/que-pasa/noticia/cientificos-chilenos-crean-innovador-spray-nasal-basado-celulas-madre-revertir-danos-cerebrales/625695/

Nota de prensa EMOL, Vinculación con el medio: Investigan spray nasal que permitiría revertir los daños cerebrales de la asfixia al nacer. https://vcm.emol.com/5533/investigacion/danos-cerebrales-de-la-asfixia-al-nacer/ Abril 22, 2019.

Conferencia Mesenchymal stem cells as a novel therapeutic strategy against neonatal asphyxia. Pontificia Universidad Católica, Neonatología, Julio 27, 2019.

Nota de prensa TV Chilevision -CNN Más que un recuerdo de infancia: Dientes de leche podrían ser una solución médica gracias a las células madres https://www.futuro360.com/videos/dientes-leche-celulas-madres_20190410/ 1/10 Abril, 10, 2019

Conicyt 1000 cientificos 1000 aulas Título charla: Nuevas neuronas en el cerebro: implicancia en el aprendizaje y la reparación cerebral Fecha de la charla: 27-09-2019 02:00 Establecimiento: Escuela el Renoval, Concepción.

Nota de prensa Facultad de Medicina, Universidad de Chile Spray nasal basado en secretomas de células madres revierte los daños cerebrales inducidos por la asfixia perinatal Abril 12, 2019. http://www.medicina.uchile.cl/noticias/152768/spray-nasal-de-secretomas-revierte-dano-cerebral-por-asfixia-perinatal#

Nota de prensa Vista previa Noticias de la Facultad e Medicina Spray nasal basado en secretomas de células madres revierte los daños cerebrales inducidos por la asfixia perinatal. Abril 12, 2019.
http://www.uchile.cl/noticias/152974/spray-nasal-de-secretomas-revierte-dano-cerebral-por-asfixia-perinatal

Nota de Prensa Facultad de Medicina: Proyecto Fondecyt Regular 2019 Spray nasal basado en secretomas de células madres revierte los daños cerebrales inducidos por la asfixia perinatal. Abril 12, 2019. http://www.medicina.uchile.cl/noticias/spray-nasal-de-secretomas-revierte-dano-cerebral-por-asfixia-perinatal

Nota de Prensa Universidad de Chile: Avances médicos: Investigadora estudia spray nasal que permitiría revertir los daños cerebrales inducidos por la asfixia perinatal Abril 18, 2019. https://www.uchile.cl/noticias/152974/spray-nasal-de-secretomas-revierte-dano-cerebral-por-asfixia-perinatal

Nota de prensa Diario Publimetro: La salvación para muchos: científicos de la Universidad de Chile crearon un spray nasal para combatir el alcoholismo. Agosto, 28, 2018. https://www.publimetro.cl/cl/noticias/2018/08/28/la-salvacion-muchos-cientificos-la-universidad-chile-crearon-spray-nasal-combatir-alcoholismo.html?fbclid=IwAR1vcPCQHDoulM4qePdJ0j7pk87cRfIkvyn8hdrwvgzDnXjDqRfj5tJocd8

Nota de prensa Noticias Universidad de Chile: Científicos de la U. de Chile crean spray nasal para el tratamiento del alcoholismo. http://www.uchile.cl/noticias/146492/cientificos-de-la-ude-chile-crean-spray-nasal-para-tratar-alcoholismo. Agosto, 28, 2018.

Entrevista radial ADN radio: Científicos de la U. de Chile crean spray nasal para combatir el alcoholismo
Para obtener buenos resultados, los investigadores experimentaron con células madres en ratas. Agosto, 28, 2018. https://www.adnradio.cl/noticias/sociedad/cientificos-de-la-u-de-chile-crean-spray-nasal-para-combatir-el-alcoholismo/20180828/nota/3791903.aspx

Participation in scientific meetings of the National Service for the Prevention and Rehabilitation of Drug and Alcohol Consumption-SENDA (Ministry of the Interior & Public Security, Chile) Consultant scientist (2015-2017)

Diario la Tercera, Tendencias Científicos chilenos logran frenar consumo excesivo de alcohol con células madre Noviembre, 11, 2016. https://www.latercera.com/noticia/cientificos-chilenos-logran-frenar-consumo-excesivo-alcohol-celulas-madre/

Project Supervision and Scientific Research Unit Liceo de Niñas No 1, Santiago Chile (Program directed by Prof. Roxana Nahuelcura) (3 students, 2, 3 and 4 medium) 2016

Conference Program 1000 scientists 1000 classrooms, Conycyt Explora. Students 3-4 media. New neurons implication in learning and brain repair. Evangelical Education Center of Hualpén, Concepción, Oct 7, 2016. Explora Conicyt.

Participation Explora -Conicyt Prog 1000 scientists 1000 classrooms 2010-2016

La Tercera Daily : Article Chilean scientists succeed in stoped excessive alcohol consumption with stem cells, 11-10.2016

Communication Website of the Faculty of Medicine, University of Chile. With stem cells they are able to stop excessive alcohol consumption, 10-11-2016

Presentation of the conference ICBM for schools students: new neurons: learning and brain repair Extension program School of Medicine, University of Chile 9-28-2016

Conference: Effects of dopamine on the postnatal neurogenesis Karolinska Institutet, Stockholm Sweden, July-2015

Conference: Effects of perinatal asphyxia on the postnatal neurogenesis at Karolinska Institutet, Stockholm Sweden Jun-2014

Internship in Karolinska Institutet, Lab Ernest Arenas July-August, 2015 International collaboration and dissemination. High-throughput primary dopamine cell cultures. Neurogenesis and dopamine.

Internship in Karolinska Institutet, Lab Tomas Hokfeld June-July, 2014 International collaboration and dissemination. Study of secretagogin in neurogenic regions. Effect of dopaminergic denervation.

Interview Faculty of Medicine How the brain is repaired after perinatal asphyxia, August, 2011

Lecture: Acute and long-term effects induced by perinatal asphyxia focusing in neurogenesis at Central Institute of Mental Health, Dept. of Psychopharmacology, Mannheim, Germany. March 2011.

Internship at the European Neuroscience Institute Göttingen, University Goettingen, Germany, Lab Andre Fischer, Epigenetics and laser capture. June-July 2012

Internship in ZI Mannheim, LabPeter Gebicke-Haerter, Rainer Spanaguel Epigenetic study of samples obtained from rats with perinatal asphyxia and controls at different ages. Evaluation by qPCR, ChIP and confocal microscopy June 7-July 8 2011

Conferences: Pathophysiology of perinatal asphyxia: can we predict and improve individual outcomes ?. ZI Mannheim

Lecture Neurogenesis and Perinatal Asphyxia: a therapeutic strategy at European Neuroscience Institute Göttingen, University Goettingen, Germany June 11, 2011.

Interview Bioplanet Magazine Perinatal asphyxia: Role of dopamine receptors November 2011

Postdoc fellowship in Canada in the laboratory of Professor R. Andrew Tasker, Department of Biomedical Sciences, University of Prince Edward Island, Charlottetown, PEI, Canada. Study of the branching of the mossy fibers of the dentate gyrus of the hippocampus. January, 2007