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Nelson Patricio Barrera Rojas

Associate Professor

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

Líneas de Investigación


Membrane Protein structure and function, Membrane Protein Structure by Atomic Force Microscopy, Membrane Protein Structure by Mass Spectrometry, Signal transduction of membrane proteins, Receptor Cross-talk, Single-molecule events, Nanophysiology

Educación

  •  Biochemistry, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 1997
  •  Biochemistry, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 1999
  •  Biological Sciences with major in Physiology, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2004

Experiencia Académica

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    2009 - 2018

  •   Visiting Professor Other

    UNIVERSITY OF OXFORD

    Oxford, Reino Unido

    2011 - 2011

  •   Research Associate Other

    UNIVERSITY OF CAMBRIDGE

    Cambridge, Reino Unido

    2004 - 2007

  •   Research Associate Other

    UNIVERSITY OF CAMBRIDGE

    Cambridge, Reino Unido

    2007 - 2009

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Chile

    2018 - A la fecha

Experiencia Profesional

  •   Assistant Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    2009 - 2018

  •   Associate Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    2018 - A la fecha

Formación de Capital Humano


Undergraduate and Master Thesis (in Progress)

José Cordero, BSc in Biochemistry, Title ‘AFM applications on cellular and molecular biomechanics’. Pontificia Universidad Católica de Chile. Since September 2017.

Graciela Bravo, BSc in Biochemistry, Title ‘Effect of magnetic nanorods on cellular morphology’. Pontificia Universidad Católica de Chile. Since August 2017. (co-tutor)

Christian Fuentes, Mac in Physics, Title 'Studies of P2X4 receptor dynamics upon agonist activation by High-Speed Atomic Force Microscopy and Molecular Dynamics simulations'. Pontificia Universidad Católica de Chile. Since August 2017.

Thesis (Finished)

Gabriel Olguín, BSc in Bioinformatics, Title ‘Molecular dynamics simulations on IgG1 adsorption onto kaolinite surface’. Universidad de Talca. October 2017.

Camila Cárdenas, BSc in Biochemistry, Title ‘Characterization of the interaction between P2X2 receptor and alpha-[2-Azidoetil]-ATP via AFM and patch-clamp'. Pontificia Universidad Católica de Chile. March 2016.

Eduardo Santander, BSc in Biochemistry and Pharmacy, Title ‘AFM application on adsorption of membrane proteins'. Pontificia Universidad Católica de Chile. March 2015.

PhD Thesis in Progress

Nicole Morales, PhD(c) in Biological Sciences, major Physiology, Title ‘Biomechanical characterization of the NET-endothelium interaction’. Pontificia Universidad Católica de Chile. Since July 2016.

Paola Soto, PhD(c) in Biological Sciences, major Physiology, Title ‘AFM determination of the crosstalk between 5HT3A and P2X2 receptors'. Pontificia Universidad Católica de Chile. Since September 2014.

Post-Doctoral Supervision

Pamela Naulin, Title ‘Molecular architecture of heteromeric Cx26/Cx30 hemichannels’. From November 2015 until November 2018, FONDECYT Postdoctoral (Sponsor Dr. Nelson Barrera)

Pablo Gaete, Title ‘Molecular regulation of the pannexin-1 in endothelial cells’. From November 2015 until November 2018, FONDECYT Postdoctoral (Sponsor Dr. Nelson Barrera)

Karla Droguett, Title ‘Biomechanical properties of the respiratory epithelium induced by pro-immflamatory processes'. Since November 2014 until November 2017, FONDECYT Postdoctoral (Sponsor Dr. Nelson Barrera)

Gean Carlos Arteaga, Title ‘Synthesis of P2X2 receptor ligands for single molecule force spectroscopy measurements’. Since January 2014 until December 2014, PUC Interdisciplinary Postdoctoral (Co-Sponsor Dr. Nelson Barrera)

Andrea Gómez, Title ‘Structural analysis of the LHC complex’. Since November 2013 until November 2016, FONDECYT Postdoctoral (Sponsor Dr. Nelson Barrera)


Premios y Distinciones

  •   Outstanding Contribution Award

    UNIVERSITY OF CAMBRIDGE

    Reino Unido, 2008

    Work on Mass spectrometry of membrane proteins at the laboratory of Carol Robinson

  •   Isaac Newton Institute Award

    ISAAC NEWTON INSTITUTE

    Reino Unido, 2007

    Participación en workshop ‘Effective Computational Methods for Highly Oscillatory Problems: The Interplay between Mathematical Theory and Applications


 

Article (48)

Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion
Activation of P2X4 receptors induces an increase in the area of the extracellular region and a decrease in receptor mobility
Function of P2X4 Receptors Is Directly Modulated by a 1:1 Stoichiometric Interaction With 5-HT(3)A Receptors
Live endothelial cells on plasma-nitrided and oxidized titanium: An approach for evaluating biocompatibility
Polydisperse molecular architecture of connexin 26/30 heteromeric hemichannels revealed by atomic force microscopy imaging
Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles
Inhibition of Wild Enterobacter cloacae Biofilm Formation by Nanostructured Graphene- and Hexagonal Boron Nitride-Coated Surfaces
Nanoscale Mobility of the Apo State and TARP Stoichiometry Dictate the Gating Behavior of Alternatively Spliced AMPA Receptors
Structural characterization of the saxitoxin-targeting APTSTX1 aptamer using optical tweezers and molecular dynamics simulations
An autocrine ATP release mechanism regulates basal ciliary activity in airway epithelium
Combining Mass Spectrometry and X-Ray Crystallography for Analyzing Native-Like Membrane Protein Lipid Complexes
Modified Electrodes with Polymers Based on Aniline and Thiophene: Characterization and Possible Use as a Sensor
P2X4 Receptor in Silico and Electrophysiological Approaches Reveal Insights of Ivermectin and Zinc Allosteric Modulation
Study of the Ultrastructure of Eucalyptus globulus Wood Substrates Subjected to Auto-Hydrolysis and Diluted Acid Hydrolysis Pre-treatments and Its Influence on Enzymatic Hydrolysis
Low physiological levels of prostaglandins E-2 and F-2 improve human sperm functions
Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore
Toward atomic force microscopy and mass spectrometry to visualize and identify lipid rafts in plasmodesmata
alpha-Amino-3-hydroxy-5-methyl-4-isoxazole Propionic Acid (AMPA) and N-Methyl-D-aspartate (NMDA) Receptors Adopt Different Subunit Arrangements
Mass Spectrometry-From Peripheral Proteins to Membrane Motors
Evidence for the Assembly of a Bacterial Tripartite Multidrug Pump with a Stoichiometry of 3:6:3
Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy.
Mass Spectrometry of Intact V-Type ATPases Reveals Bound Lipids and the Effects of Nucleotide Binding
Opening of the Outer Membrane Protein Channel in Tripartite Efflux Pumps Is Induced by Interaction with the Membrane Fusion Partner
Ion Mobility Mass Spectrometry of Two Tetrameric Membrane Protein Complexes Reveals Compact Structures and Differences in Stability and Packing
MDMA ("ecstasy") impairs learning in the Morris Water Maze and reduces hippocampal LTP in young rats
Separating and visualisiing protein assemblies by means of preparative mass spectrometry and microscopy
A Multidrug ABC Transporter with a Taste for Salt
MacB ABC Transporter Is a Dimer Whose ATPase Activity and Macrolide-binding Capacity Are Regulated by the Membrane Fusion Protein MacA
Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions
Atomic Force Microscopy Reveals the Stoichiometry and Subunit Arrangement of the  4 3  GABAA Receptor
Automated analysis of the architecture of receptors, imaged by atomic force microscopy
Determination of the architecture of ionotropic receptors using AFM imaging
Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging
Micelles Protect Membrane Complexes from Solution to Vacuum
Providing unique insight into cell biology via atomic force microscopy
The subunit arrangement and assembly of ionotropic receptors
AFM imaging reveals the tetrameric structure of the TRPC1 channel
ATP and adenosine trigger the interaction of plasma membrane IP3 receptors with protein kinase A in oviductal ciliated cells
The Stoichiometry of P2X2/6 Receptor Heteromers Depends on Relative Subunit Expression Levels
An Uncharged Region within the N-Terminus of the P2X6 Receptor Inhibits Its Assembly and Exit from the Endoplasmic Reticulum
Class of skew-distributions: Theory and applications in biology
Atomic Force Microscopy Imaging Demonstrates that P2X2 Receptors Are Trimers but That P2X6 Receptor Subunits Do Not Oligomerize
Atomic force microscopy reveals the stoichiometry and subunit arrangement of 5-HT3 receptors
Plasma and intracellular membrane inositol 1,4,5-trisphosphate receptors mediate the Ca2+ increase associated with the ATP-induced increase in ciliary beat frequency
Prediction of synergism on frequency of responses in the attojoule range
Platelet activating factor increases ciliary activity in the hamster oviduct through epithelial production of prostaglandin E-2
Platelet activating factor increases ciliary activity in the hamster oviduct through epithelial production of prostaglandin E2
Functional cross talk after activation of P2 and P1 receptors in oviductal ciliated cells

Abstract (12)

Regulation of Pannexin-1 Channel Gating by Nitric Oxide and Camp Signaling
Combined in silico plus electrophysiological studies identify molecular determinants of ivermectin and zinc P2X4R allosterism
Atomic Force Microscopy Imaging Reveals Structural and Mechanical Properties of Dissociated Hemocyanins by Temperature
Basal ciliary activity depends on ATP release in respiratory epithelium of mouse trachea
Structural analysis of the P2X4 receptor reveals insights of the mechanism of allosteric regulation by alfaxolone
Structural Studies of Heteromeric Connexin26/30 Hemichannels via Atomic Force Microscopy Imaging
Combined Mass Spectrometry and Molecular Dynamics to Study Membrane Protein Stability in Gas Phase
Single Molecule Cross-Talk Transferred from a Molecular Event to an Organ Function
A physical chemistry theory of synergism on ciliated cell motility
Synergism on ciliary activity induced by ATP and adenosine depends on a reciprocal modulation of their transduction pathways
Arachidonic acid is a mediator of the increase on ciliary activity induced by PAF in hamster oviductal epithelium.
Transduction mechanisms associated with activation of P-1 and P-2 purinoceptors in oviductal ciliated cells: functional cross-talk between both pathways

BookSection (2)

Providing Unique Insight into Cell Biology via Atomic Force Microscopy
Reciprocal Synergism at Early Stages of Transduction Pathways Allows Efficient Cell Signalling

Proyecto (13)

Aplicación de Espectrometría de Masas de Alta Resolución para potenciar estudios interdisciplinarios de proteómica, biología estructural y biomedicina=> desde péptidos hasta complejos supramoleculares intactos
Desarrollo de un metodo integrado de medicion de las propiedades biomecanicas de celulas vivas y biomateriales=> una nueva aproximacion para evaluar biocompatibilidad
CONTROL AND COORDINATION OF MICROVASCULAR VASOMOTOR TONE BY NITRIC OXIDE-DEPENDENT REGULATION OF CONNEXIN- AND PANNEXIN-FORMED GAP JUNCTION CHANNELS OR HEMICHANNELS
Determinación de la arquitectura molecular y funcional de los hemicanales de conexinas heteromericas Cx26/Cx30
High-Speed Analysis of Cross-Talk between Neurotransmitter-Gated Channels
Regulacion molecular de las dinamicas de apertura del canal formado por Panexina-1 en la celula endotelial=> La respuesta gatillada por CGRP
PROCESOS PRO-INFLAMATORIOS MODIFICAN LAS PROPIEDADES BIOMEC�NICAS CELULARES QUE REGULAN EL TRANSPORTE MUCOCILIAR EN EL EPITELIO RESPIRATORIO
ANALISIS ESTRUCTURAL DEL COMPLEJO MODIFICADOR DE CROMATINA LHC MEDIANTE ESPECTROMETRIA DE MASA Y MICROSCOPIA DE FUERZA ATOMICA.
Nano-devices for high precision measurements in biology and nano structured materials for biomedicine
Single molecule pharmacology of P2X receptors by atomic force microscopy
The Membrane Protein Structural Biology Millennium Nucleus
Role of detergent micelles on the stoichiometry of intact membrane proteins in the gas phase
Dynamic multiprotein complexes-insights from mass spectrometry

Review (4)

Vas deferens neuro-effector junction: From kymographic tracings to structural biology principles
The role of lipids in defining membrane protein interactions: insights from mass spectrometry
Advances in the Mass Spectrometry of Membrane Proteins: From Individual Proteins to Intact Complexes
Principles: Mechanisms and modeling of synergism in cellular responses
65
Nelson Barrera

Associate Professor

Physiology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

11
Bernardo Morales

Profesor titular

Biologia

Universidad de Santiago de Chile

Santiago, Chile

2
Sebastián Aguayo

Assistant Professor

Dentistry

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Christina Schuh

Assistant Professor

Centre for Regenerative Medicine

Universidad del Desarrollo

Santiago, Chile

2
Mariana Rios

Investigador

Facultad de Ciencias Biológicas

pontificia Universidad Católica de Chile

Santiago, Chile

2
Nathalie Casanova

profesor asociado

artes liberales

Universidad Adolfo Ibañez

Santiago, Chile

1
Eduardo Troncoso

Técnico

Centro de Biotecnología

Universidad de Concepción

Concepción, Chile

1
Pablo Conejeros

Profesor Adjunto

Universidad de Valparaiso

Valparaiso, Chile

1
Maria Godoy

Coordinadora Científica

ANID

Santiago, Chile

1
Xavier Figueroa

Associate Professor

Departamento de Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Andres Ramirez

Profesor Asistente

Facultad de Ciencias

Universidad Mayor de Chile

Temuco, Chile

1
Francisco Montero

Investigador Responsable, pryecto FOndecyt postdoctorado 3180683

Química

Universidad Austral de Chile

Valdivia, Chile

1
Ricardo Henriquez

Professor

Physics

UNIVERSIDAD TECNICA FEDERICO SANTA MARIA

Valparaiso, Chile

1
Carolina Parra

Young Researcher. Head of Nanobiomaterials Laboratory

Physics

UNIVERSIDAD TECNICA FEDERICO SANTA MARIA

Valparaíso, Chile

1
Valeria del Campo

Investigador

Física

UNIVERSIDAD TÉCNICA FEDERICO SANTA MARÍA

Valparaiso, Chile

1
Marcela Wilkens

Profesor Asociado

Biología

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

1
Cristian Ramírez

Associated researcher

Chemical and Environmental Engineering Department

UNIVERSIDAD TECNICA FEDERICO SANTA MARIA

Valparaiso, Chile

1
Sheila Lascano

Profesor Auxiliar

Ingeniería Mecánica

UNIVERSIDAD TECNICA FEDERICO SANTA MARIA

Santiago de Chile, Chile

1
Juan Araya

Instructor

Análisis Instrumental

Universidad de Concepción

Concepción, Chile

1
Juan Pablo Garcia Huidobro

Full Professor

Biology

Universidad de Santiago de Chile

Santiago, Chile

1
CARLOS PEÑA

PROFESOR

INSTITUTO DE CIENCIAS QUIMICAS APLICADAS

Universidad Autónoma de Chile

TEMUCO, Chile

1
Sebastián Loyola

Académico adjunto

Ciencias de la Salud

Universidad Católica Silva Henriquez

Santiago, Chile

1
Ricardo Cabrera

Assitant Pofessor

Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Miguel Reyes

Full Professor

Facultad de Ciencias Médicas

UNIVERSIDAD DE SANTIAGO DE CHILE, FACULTAD DE CIENCIAS MÉDICAS, ESCUELA DE MEDICINA

Santiago, Chile

1
Bruna Benso

Profesora Asistente

Facultad de Medicina

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Floria Pancetti

Director de Departamento

Ciencias Biomédicas

UNIVERSIDAD CATOLICA DEL NORTE

Coquimbo, Chile

1
Marcela Hermoso

Full Professor

School of Medicine

UNIVERSIDAD DE CHILE

Santiago, Chile

1
simon alvarez

Asistente de investigación

Facultad de Medicina

Universidad del Desarrollo

Santiago, Chile