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Eduardo Felipe Fuentes Lemus

Profesor Asistente

Pontificia Universidad Católica de Chile

Santiago, Chile

Protein oxidation; Protein glycation; Protein cross-linking; Reactive oxygen species; LC-MS proteomics; Kinetics and mechanisms of oxidative damage to proteins in crowded environments

Doctor en Química, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2018
Químico Farmacéutico, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2014

Postdoc Full Time

UNIVERSIDAD DE SANTIAGO DE CHILE

Facultad de Química y Biología

Chile

2019 - 2020
Postdoc Full Time

University of Copenhagen

Faculty of Health and Medical Sciences

Copenhagen, Dinamarca

2020 - 2024
Profesor Asistente Full Time

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Facultad de Química y de Farmacia

Santiago, Chile

2024 - A la fecha

Postdoc Full Time

University of Copenhagen

Copenhagen, Dinamarca

2020 - 2024
Guest Researcher Other

University of Copenhagen

Copenhagen, Chile

2024 - A la fecha
Profesor Asistente Full Time

Pontificia Universidad Católica de Chile

Santiago, Chile

2024 - A la fecha

PREMIO A LA EXCELENCIA EN TESIS DOCTORAL - VRI

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Chile, 2018

Este premio tiene por objetivo reconocer y estimular la investigación desarrollada por alumnos/as de los distintos Programas de Doctorado de la Universidad, cuyas tesis doctorales representen un aporte a la proyección de las ciencias, humanidades, artes y tecnologías de nuestro país. Area Premio: Ciencias Químicas, Físicas y Matemáticas.
SFRR-EUROPE CATHERINE PASQUIER MEMORIAL AWARD

Society for Free Radical Research Europe

Dinamarca, 2024

The Catherine Pasquier Memorial Award Lecture is an annual award honouring the life of Catherine Pasquier of INSERM, Paris, and President of SFRR Europe from 2001-2002. The eligibility criteria have been selected to reflect what we know were Professor Catherine Pasquier’s priorities during her prolific career. The eligible person must be an Early Career Scientist: 1) Beyond formal training but not yet fully established. 2) From any country in Europe and an active member of SFRR Europe. 3) With career accomplishments and therefore also with future promise. 4) With achievements in the broad area of free radical research. This may include work at the basic, clinical or epidemiological level. This award is, thus, not intended for well-established investigators or as recognition for a single project, but for overall accomplishments and future promise. The required material are the candidate's full CV with an up-to-date publications list and a nominator's letter of recommendation. This letter is very important and should clearly lay out the importance of the candidate's accomplishments to date and be convincing in its prediction of future research promise. An SFRR Europe Catherine Pasquier Scientific Committee, comprised of the the SFRR Europe Executive and chaired by the President-Elect, selects the Award Lecture based on the received nominations with the awardee fulfilling the eligibility criteria, in particular their research excellence and promise and without regard to nationality.
MSCA-IF-2019 - Individual Fellowships

European Commission

Chile, 2020

EXCELLENT SCIENCE - Marie Sk?odowska-Curie Actions


Oxidation of the active site cysteine residue of glyceraldehyde-3-phosphate dehydrogenase to the hyper-oxidized sulfonic acid form is favored under crowded conditions Glover, Mia R.; Davies, Michael J.; Fuentes-Lemus, Eduardo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2024)
Complexation of AAPH (2,2'-azobis(2-methylpropionamidine) dihydrochloride) with cucurbit[7]uril enhances the yield of AAPH-derived radicals Forero-Giron, Angie C.; Fuentealba, Denis; Marino-Ocampo, Nory; Gutierrez-Oliva, Soledad; Herrera, Barbara; Toro-Labbe, Alejandro; Fuentes-Lemus, Eduardo; Davies, Michael J.; Aliaga, Margarita E.; Lopez-Alarcon, Camilo Article ISI JOURNAL OF MOLECULAR LIQUIDS (2023)
Effect of crowding, compartmentalization and nanodomains on protein modification and redox signaling-current state and future challenges Fuentes-Lemus, Eduardo; Davies, Michael J. Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2023)
From workout to molecular switches: How does skeletal muscle produce, sense, and transduce subcellular redox signals? Henriquez-Olguin, Carlos; Meneses-Valdes, Roberto; Kritsiligkou, Paraskevi; Fuentes-Lemus, Eduardo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2023)
Oxidant-modified amylin fibrils and aggregates alter the inflammatory profile of multiple myeloid cell types, but are non-toxic to islet β cells Clemen, Ramona; Fuentes-Lemus, Eduardo; Bekeschus, Sander; Davies, Michael J. Article ISI REDOX BIOLOGY (2023)
Oxygen Exposure and Tolerance Shapes the Cell Wall-Associated Lipids of the Skin Commensal Cutibacterium acnes Popa, Iuliana; Touboul, David; Andersson, Tilde; Fuentes-Lemus, Eduardo; Santerre, Cyrille; Davies, Michael J.; Lood, Rolf Article ISI Microorganisms (2023)
Peroxyl radicals modify 6-phosphogluconolactonase from Escherichia coli via oxidation of specific amino acids and aggregation which inhibits enzyme activity Reyes, Juan Sebastian; Fuentes-Lemus, Eduardo; Romero, Jefferson; Arenas, Felipe; Fierro, Angelica; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2023)
The cysteine residue in beta-lactoglobulin reacts with oxidized tyrosine residues in beta-casein to give casein-lactoglobulin dimers Doblas, Laura; Hagglund, Per M.; Fuentes-Lemus, Eduardo; Davies, Michael J. Article ISI ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2023)
Crowding modulates the glycation of plasma proteins:<i> In</i><i> vitro</i> analysis of structural modifications to albumin and transferrin and identification of sites of modification Fuentes-Lemus, Eduardo; Reyes, Juan S.; Lopez-Alarcon, Camilo; Davies, Michael J. Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2022)
Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway Sebastian Reyes, Juan; Fuentes-Lemus, Eduardo; David Figueroa, Juan; Rojas, Javier; Fierro, Angelica; Arenas, Felipe; Hagglund, Per M.; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI SCIENTIFIC REPORTS (2022)
Oxidant-mediated modification and cross-linking of beta-2-microglobulin Jiang, Shuwen; Fuentes-Lemus, Eduardo; Davies, Michael J. Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2022)
Reaction of cysteine residues with oxidized tyrosine residues mediates cross-linking of photo-oxidized casein proteins Rossi, Chiara; Fuentes-Lemus, Eduardo; Davies, Michael J. Article ISI FOOD CHEMISTRY (2022)
Role of amino acid oxidation and protein unfolding in peroxyl radical and peroxynitrite-induced inactivation of glucose-6-phosphate dehydrogenase from <i>Leuconostoc mesenteroides</i> David Figueroa, Juan; Fuentes-Lemus, Eduardo; Sebastian Reyes, Juan; Loaiza, Matias; Aliaga, Margarita E.; Fierro, Angelica; Leinisch, Fabian; Hagglund, Per; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2022)
Effect of macromolecular crowding on protein oxidation: Consequences on the rate, extent and oxidation pathways Fuentes-Lemus, Eduardo; Sebastian Reyes, Juan; Gamon, Luke F.; Lopez-Alarcon, Camilo; Davies, Michael J. Article ISI REDOX BIOLOGY (2021)
High concentrations of casein proteins exacerbate radical chain reactions and increase the extent of oxidative damage Fuentes-Lemus, Eduardo; Jiang, Shuwen; Hagglund, Per; Davies, Michael J. Article ISI FOOD HYDROCOLLOIDS (2021)
M. jannaschii FtsZ, a key protein in bacterial cell division, is inactivated by peroxyl radical-mediated methionine oxidation Reyes, Juan Sebastian; Fuentes-Lemus, Eduardo; Aspee, Alexis; Davies, Michael J.; Monasterio, Octavio; Lopez-Alarcon, Camilo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2021)
Oxidation of lysozyme induced by peroxyl radicals involves amino acid modifications, loss of activity, and formation of specific crosslinks Fuentes-Lemus, Eduardo; Mariotti, Michele; Hagglund, Per; Leinisch, Fabian; Fierro, Angelica; Silva, Eduardo; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2021)
Azocompounds as generators of defined radical species: Contributions and challenges for free radical research Lopez-Alarcon, Camilo; Fuentes-Lemus, Eduardo; Figueroa, Juan David; Dorta, Eva; Schoeneich, Christian; Davies, Michael J. Article ISI FREE RADICAL BIOLOGY AND MEDICINE (2020)
Formation and characterization of crosslinks, including Tyr-Trp species, on one electron oxidation of free Tyr and Trp residues by carbonate radical anion David Figueroa, Juan; Maria Zarate, Ana; Fuentes-Lemus, Eduardo; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI RSC ADVANCES (2020)
Free radicals derived from ?-radiolysis of water and AAPH thermolysis mediate oxidative crosslinking of eGFP involving Tyr-Tyr and Tyr-Cys bonds: the fluorescence of the protein is conserved only towards peroxyl radicals Zamora R.A.; Fuentes-Lemus E.; Barrias P.; Herrera-Morande A.; Mura F.; GuixÃ© V.; Castro-Fernandez V.; Rojas, T; LÃ³pez-AlarcÃ³n C.; Aguirre P.; Rivas-Aravena A.; AspÃ©e A. Article ISI SCOPUS Free Radical Biology and Medicine (2020)
Photo-induced protein oxidation: mechanisms, consequences and medical applications Fuentes-Lemus, Eduardo; Lopez-Alarcon, Camilo; Hooper, N Article ISI NON-CODING GENOME (2020)
Photo-oxidation of lysozyme triggered by riboflavin is O2-dependent, occurs via mixed type 1 and type 2 pathways, and results in inactivation, site-specific damage and intra- and inter-molecular crosslinks Fuentes-Lemus E.; Mariotti M.; Reyes J.; Leinisch F.; HÃ¤gglund P.; Silva E.; Davies M.J.; LÃ³pez-AlarcÃ³n C. Article ISI SCOPUS Free Radical Biology and Medicine (2020)
Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times Cortes-Rios, Javiera; Maria Zarate, Ana; David Figueroa, Juan; Medina, Joaquin; Fuentes-Lemus, Eduardo; Rodriguez-Fernandez, Maria; Aliaga, Margarita; Lopez-Alarcon, Camilo Article ISI ANALYTICAL BIOCHEMISTRY (2020)
Binding of rose bengal to lysozyme modulates photooxidation and cross-linking reactions involving tyrosine and tryptophan Fuentes-Lemus, Eduardo; Mariotti, Michele; Hägglund, Per; Leinisch, Fabian; Fierro, Angélica; Silva, Eduardo; López-Alarcón, Camilo; Davies, Michael J. Article ISI SCOPUS FREE RADICAL BIOLOGY AND MEDICINE (2019)
Oxidation of myofibrillar proteins induced by peroxyl radicals: Role of oxidizable amino acids Dorta, E.; Avila, F.; Fuentes-Lemus, E.; Fuentealba, D.; Lopez-Alarcon, C. Article ISI SCOPUS FOOD RESEARCH INTERNATIONAL (2019)
Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching David Figueroa, Juan; Fuentes-Lemus, Eduardo; Dorta, Eva; Melin, Victoria; Cortes-Rios, Javiera; Faundez, Mario; Contreras, David; Denicola, Ana; Alvarez, Beatriz; Davies, Michael J.; Lopez-Alarcon, Camilo Article ISI SCOPUS REDOX BIOLOGY (2019)
Riboflavin-induced Type 1 photo-oxidation of tryptophan using a high intensity 365 nm light emitting diode Silva, E; Barrias, P; Fuentes-Lemus, E; Tirapegui, C; Aspee, A; Carroll, L; Davies, MJ; Lopez-Alarcon, C Article ISI SCOPUS FREE RADICAL BIOLOGY AND MEDICINE (2019)
Aggregation of alpha- and beta- caseins induced by peroxyl radicals involves secondary reactions of carbonyl compounds as well as di-tyrosine and di-tryptophan formation Fuentes-Lemus, E; Silva, E; Barrias, P; Aspee, A; Escobar, E; Lorentzen, LG; Carroll, L; Leinisch, F; Davies, MJ; Lopez-Alarcon, C Article ISI SCOPUS FREE RADICAL BIOLOGY AND MEDICINE (2018)
alpha- and beta-casein aggregation induced by riboflavin-sensitized photo-oxidation occurs via di-tyrosine cross-links and is oxygen concentration dependent Fuentes-Lemus, E; Silva, E; Leinisch, F; Dorta, E; Lorentzen, LG; Davies, MJ; Lopez-Alarcon, C Article ISI SCOPUS FOOD CHEMISTRY (2018)
Use of the Oxygen Radical Absorbance Capacity (ORAC) Assay to Predict the Capacity of Mango (Mangifera indica L.) By-Products to Inhibit Meat Protein Oxidation Dorta, E; Rodriguez-Rodriguez, EM; Jimenez-Quezada, A; Fuentes-Lemus, E; Speisky, H; Lissi, E; Lopez-Alarcon, C Article FOOD ANALYTICAL METHODS (2017)
Oxidation of free, peptide and protein tryptophan residues mediated by AAPH-derived free radicals: role of alkoxyl and peroxyl radicals Fuentes-Lemus, E; Dorta, E; Escobar, E; Aspée A.; Pino E.; Abasq M.L.; Speisky H.; Silva, E.; Lissi E.; Davies, M. J; López-Alarcón C. Article ISI SCOPUS RSC ADVANCES (2016)
Use of the Oxygen Radical Absorbance Capacity (ORAC) Assay to Predict the Capacity of Mango (Mangifera indica L.) By-Products to Inhibit Meat Protein Oxidation Dorta, E; Rodríguez-Rodríguez E.M.; Jiménez-Quezada A.; Fuentes-Lemus, E; Speisky H.; Lissi E.; López-Alarcón C. Article SCOPUS Food Analytical Methods (2016)
The ORAC (oxygen radical absorbance capacity) index does not reflect the capacity of antioxidants to trap peroxyl radicals Dorta, E; Fuentes-Lemus, E; Aspée A.; Atala E.; Speisky H.; Bridi R.; Lissi E.; Lopez-Alarcon, C Article ISI SCOPUS JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING (2015)


Estudio del rol de las mezclas de caseínas sobre el grado y mecanismo de oxidación de estas proteínas mediado por los radicales peroxilo Dirección de Investigación Científica y Tecnológica Universidad de Santiago de Chile (021941AL_POSTDOC) Proyecto 2019-2019 Postdoctorado
Oxidative modifications induced by carbonate radical, nitrogen dioxide, and peroxyl radicals on two enzymes of the pentose phosphate pathway=> consequences on NADPH release and reduced glutathione recycling FONDECYT (n°1180642) Proyecto 2018-2020 Personal técnico y/o de apoyo
Role of Tryptophan residues in the aggregation and fragmentation of peptides and proteins induced by peroxyl and carbon-centered radicals. FONDECYT (1141142) Proyecto 2014-2018 Tesista
Extending the use of Pyrogallol Red as probe in methodologies aimed to estimate the scavenging activity of beverages and foods towards Nitrite, Hypochlorous acid, Chloramines and Peroxyl radicals. FONDECYT (1100659) Proyecto 2010-2014 Tesista


Use of photosensitive molecules in the crosslinking of biopolymers: applications and considerations in biomaterials development Santos, Nicolas; Fuentes-Lemus, Eduardo; Ahumada, Manuel Review ISI JOURNAL OF MATERIALS CHEMISTRY B (2024)
The enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species consequences for NADPH production Fuentes-Lemus, Eduardo; Reyes, Juan Sebastian; Figueroa, Juan David; Davies, Michael J.; Lopez-Alarcon, Camilo Review ISI BIOCHEMICAL SOCIETY TRANSACTIONS (2023)
Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification Fuentes-Lemus, Eduardo; Hagglund, Per; Lopez-Alarcon, Camilo; Davies, Michael J. Review ISI MOLECULES (2022)