Radicales Libres, Proteínas, Péptidos, antioxidantes

DOCTORATE SUPERVISOR
2013
Dr. Andrea Arenas, thesis: Evaluation of the oxidative damage induced by hydrosoluble peroxyl radicals on proteins of the ocular globe.
Doctorate in Chemistry, Pontificia Universidad Católica de Chile

2014
Dr. Elías Atala, thesis: Interaction of phenols and polyphenols with reactive species in acidic medium: a competitive kinetic study.
Doctorate in Chemistry, Pontificia Universidad Católica de Chile

2014
Dr. Julie Benavides, thesis: Interaction of hypochlorous acid and monochloramine with omeprazole, tetracycline and polyphenols.
Doctorate in Chemistry, Pontificia Universidad Católica de Chile

2015
Dr. Jael Reyes, thesis: Reaction of phenols towards nitrous acida: nitric oxide generation and possible role of nitrogen dioxide.
Doctorate in Chemistry, Pontificia Universidad Católica de Chile

Current PhD students
2015
Eduardo Fuentes: Oxidative processes of bovine milk proteins induced by peroxyl radicals: potential protective role of polyphenols.
Doctorate in Chemistry, Pontificia Universidad Católica de Chile

2014
Elizabeth Escobar: Role of aromatic amino-acids on the crosslinking and fragmentation processes of peptides and proteins mediated by alkyl and peroxyl radicals. Doctorate in Chemistry, Pontificia Universidad Católica de Chile

2017
Limberg Jaldin. Doctorate in Chemistry, Pontificia Universidad Católica de Chile

POSTDOCTORATE ADVISOR

Dra. Eva Dorta (FONDECYT nº 3140307).
Study of the oxidative damage on myofibrillar proteins mediated by hydrosoluble peroxyl radicals: tole of polyphenols obtained from peel and seed of mango (Mangifera Indica L.) as potential antioxidant compounds Pontificia Universidad Católica de Chile

UNDERGRADUATE SUPERVISOR

2008
Carolina Rojo Vergara (Co-director): Interaction of diclofenac with peroxyl radicals, influence on its passage through the skin.
Pharmacist, Pontificia Universidad Católica de Chile
Ivonne Martin Caruti (Director): Development and application of a new method to evaluate the antioxidant capacity of phenols and polyphenols.
Pharmacist, Pontificia Universidad Católica de Chile
Paula Torres Olivares (Director): Application of a new ORAC assay to evaluate ascorbic acid in human plasma.
Pharmacist, Pontificia Universidad Católica de Chile
Evelyn Mura Martínez (Director): Application of a new ORAC method to evaluate the antioxidant capacity of wines.
Pharmacist, Pontificia Universidad Católica de Chile
Felipe Duffe Pino (Director): Study of the interaction of antioxidants with a stable free radical in biodegradable polymeric micelles.
Pharmacist, Pontificia Universidad Católica de Chile
Paula Galleguillos Bustamante (Director): Application of a new ORAC method to evaluate ascorbic acid in human urine.
Pharmacist, Pontificia Universidad Católica de Chile

2009
Valeria Inostroza Guichonet (Director): Use of asthaxantin and Carotene to evaluate the antioxidant activity of oils throughout a new ORAC assay.
Pharmacist, Pontificia Universidad Católica de Chile

2011
Daniela Vásquez Mera (Director): Trapping of free radicals derived from amino acids using pre-fluorescent probes.
Pharmacist, Pontificia Universidad Católica de Chile
Fabiola Betancourt Lagos (Director): Pyrogallol red and 5-aminosalicilic acid: possible target molecules for a new ORAC assay towards nitrite in acidic medium.
Pharmacist, Pontificia Universidad Católica de Chile
Franco Colonelli Jeria (Director): Effect of linoleic acid in the antioxidant activity of nitroxides in neutral micelles.
Pharmacist, Pontificia Universidad Católica de Chile
Elisa Montupil Vega (Director): Spectroscopic and chromatographic study of the reaction between pyrogallol red and nitrous acid.
Pharmacist, Pontificia Universidad Católica de Chile
Max Romero Garate (Director): Effect of the compartmentalized medium on the ORAC-PGR index.
Pharmacist, Pontificia Universidad Católica de Chile

2012
Constanza Cortes Atuán (Co-director): A new method to estimate the antioxidant capacity of vegetable extracts based on the interaction of pyranine with nitrous acid. Pharmacist, Pontificia Universidad Católica de Chile
Daniel Fuentes Aravena (Director): Interaction of flavonoids with peroxyl radicals, kinetic behavior and antioxidant capacity.
Pharmacist, Pontificia Universidad Católica de Chile
César Segovia Miranda (Co-director): Nanoparticles: Interaction with free radicals.
Pharmacist, Pontificia Universidad Católica de Chile

2013
Verónica Tapia Zúñiga (Director): Interaction of pyrogallol red with superoxide. Evaluation of the antioxidant capacity of human plasma.
Pharmacist, Pontificia Universidad Católica de Chile
María Jesús Troncoso (Director): Use of the polyvinilpolipirrolidone (PVPP) for evaluating the total content of phenols in complex mixtures throughout the Folin-Ciocalteu assay. Pharmacist, Pontificia Universidad Católica de Chile
Rocío Ortíz Mercado (Director): Effect of hydrosoluble peroxyl radicals on the enzymatic activity of ?-glucosidases.
Pharmacist, Pontificia Universidad Católica de Chile
Sergio Lobato Gutiérrez (Director): Antioxidant activity of chilean red wines: application of the ORAC (Oxygen Radical Absorbance Capacity) methodology. Pharmacist, Pontificia Universidad Católica de Chile
Nelson Piro Mesina (Director): Interaction of gallic acid and esters with hypochlorite and n-chlorobutilamine: influence of a compartmentalized system. Pharmacist, Pontificia Universidad Católica de Chile
María Fernanda Morán (Co-director): Analysis of the antioxidant activity of propolis produced in the VI region, Chile.
Pharmacist, Pontificia Universidad Católica de Chile
Denisse Ovalle Jimenez (Patrocinante): Formation and implementation of a repository to the regulatory department of GlaxoSmithKline.
Pharmacist, Pontificia Universidad Católica de Chile

2014
Isaac Jara Pezoa (Co-director): Determination of the phenolic composition of propolis from VI region, Chile and their capacity to generate nitric oxide.
Pharmacist, Pontificia Universidad Católica de Chile
Claudio Villagra Soto (Co-director): Influence of cholesterol in the peroxidation of lipid vesicles containing pyrogallol red.
Pharmacist, Pontificia Universidad Católica de Chile
Romina Díaz Mosquera (Patrocinante): Study of the correlation between satisfaction of the medical consultation and the adherence to the therapy in patients with arterial hypertension. Pharmacist, Pontificia Universidad Católica de Chile
Eduardo Fuentes Lemus (Director): Consumption of tryptophan free and included in Consumo de triptófano libre e incluido en peptides mediated by peroxyl radicals: a kinetic study. Pharmacist, Pontificia Universidad Católica de Chile

CONFERENCES

Nov-2015 Society for Redox Biology and Medicine’s Annual Meeting, Boston, USA. A kinetic study of the free, peptide-included and protein-included tryptophan oxidation. E. Fuentes-Lemus, E. Dorta, M.L. Abasq, H. Speisky, E. Silva, E. Lissi, C. López-Alarcón.*

Sept-2014 Meeting of the Society Free Radical Research International (SFRR-E 2014). Paris, France. A kinetic study of tryptophan consumption mediated by peroxyl radicals: effect of its inclusion in di and tri-peptides.E. Fuentes, E. Lissi, C. López-Alarcón.*

Sept-2014 Meeting of the Society Free Radical Research International (SFRR-E 2014). Paris, France. Role of alkoxyl radicals on the fluorescein-based ORAC (Oxygen Radical Absorbance Capacity) assay. E. Dorta, E. Atala, A. Aspée, H. Speisky, E. Lissi, C. López-Alarcón.*

Oct-2013 VIII Meeting of the Society Free Radical Biology and Medicine-South American Group. A pyrogallol red-based ORAC (Oxygen Radical Absorbance Capacity) assay to evaluate antioxidant capacity of phenols in acidic medium. E. Atala, A. Aspee, H. Speisky, E. Lissi, C. López-Alarcón.

Sep.- 2012 16th biennial meeting for the Society for Free Radical Research International. Imperial College of London, UK. Bleaching of the Pyrogallol Red dye induced by superoxide anion radical: Application to study redox cycling reactions of nitrocompounds. C. López-Alarcón, H. Speisky, M. Aliaga, E. Lissi, M. Rojas, M. Faúndez.

Oct.-2011 5th International Conference on Polyphenols and Health (ICPH). Sitges, Barcelona, Spain. Interaction of pyrogallol red with hypoclorite and nitrous acid: evaluation of the antioxidant activity of pure polyphenols and their complex mixtures. C. López-Alarcón, F. Pérez-Cruz, C. Olea-Azar, E. Atala, P. Bohle, E. Lissi.

Dec.-2010 First Symposium of Free Radicals and Antioxidants. Valparaíso, Chile. In vitro evaluation of antioxidant capacity: a critical analysis. C. López-Alarcón.

Jul.-2009 Organic Free Radicals. Ottawa, Canada. Antioxidant reactivity toward nitroxides anchored in human serum albumin. A. Aspée, A. Orrego, E. Alarcón, C. López-Alarcón, H. Poblete, D. Gonzalez-Nilo.

Nov.-2008 IX Encontro Latino-Americano de Fotoquímica e Fotobiología (ELAFOT 2008). Cubatão/Santos, SP, Brazil. Determination of rate constant reactions of hydroxycinnamic acids with pyranine derived radicals by laser flash photolysis. Alexis Aspée, Luca Maretti, Gabriela Velasquez, J.C. Scaiano, Camilo López-Alarcón.

Jul.-2008 19th IUPAC Conference on Physical Organic Chemistry. Santiago de Compostela, Spain. Kinetic Study of the generation of superoxide radicals during the reaction between Cu(I)-glutathione and molecular oxygen. Margarita Aliaga, Catalina Carrasco-Pozo, Francesca Burgos, Camilo López-Alarcón, Hernán Speisky.

Oct.-2007 9th Latin American Conference on Physical Organic Chemistry. Córdoba, Argentina. Repair process in free radical reactions. E. Lissi, A. Aspée, C. Aliaga, C. López-Alarcón.

Oct.-2007 9th Latin American Conference on Physical Organic Chemistry. Córdoba, Argentina. Influence of the target molecule on the oxygen radical absorbance capacity (ORAC) index. C. López-Alarcón, I. Martin, E. Lissi.

Sep.-2007 V Meeting of Society of Free Radical Biology Medicine – South American Group. V International Conference on Peroxynitrite and Reactive Nitrogen Species. Montevideo, Uruguay. ORAC-Pyrogallol red: Evaluation of polyphenols reactivity and ascorbic acid determination in fruit extracts. C. López-Alarcón, E. Atala, H. Speisky, E. Lissi.

Jan.-2006 XXVI Congress of the Chilean Chemical Society. Concepción, Chile. “Kinetic study of the secondary reactivity of flavonoids and related polyphenols”. C. López-Alarcón, E. Lissi.

Jan.-2006 XXVI Congress of the Chilean Chemical Society. Concepción, Chile. “A novel and simple ORAC methodology based on the interaction of pyrogallol red with peroxyl radicals”. C. López-Alarcón, E. Lissi.

Sept.-2004 55 th ISE Meeting. Thessaloniki, Greece. “Electro-oxidation of 1,4-dihydropyridines and its reactivity with alkylperoxyl radicals”. L.J. Núñez-Vergara., C. López-Alarcón, J.A. Squella.

Feb.-2004 XVI Congress of the Iberoamerican Electrochemical Society (SIBAE). San José, Costa Rica. "Electro-oxidation of 1,4-dihydropyridines in aqueous medium”. C. López-Alarcón, L.J. Núñez-Vergara, J.A. Squella.

Sept.- 2003 54 th ISE Meeting. Sao Pedro, Brazil. “Voltammetric oxidation of Hantzsch 1,4-dihydropyridines in protic and aprotic media: relevance of the substitution on N position”. J. A. Squella , C. López-Alarcón, L.J. Núñez-Vergara.

Sept.-2002 XV Congress of the Iberoamerican Electrochemical Society (SIBAE). Évora, Portugal. "electrochemical oxidation of new 1,4-dihydropyridines: a GC-MS study ". L.J. Núñez-Vergara, C. López-Alarcón, C. Camargo, J.A. Squella.

Nov.-2001 XXIV Congress of the Chilean Chemical Society. Temuco, Chile. “Electro-oxidation of 1,4-DHP-4-sustituides in acetronitrile". L.J. Núñez-Vergara, C. López-Alarcón, P. Navarrete, A.M. Atria, J.A. Squella.

May.-2000 XIV Congress of the Iberoamerican Electrochemical Society (SIBAE). Oaxaca, México. “A voltammetric method to study 1,4-dihydropyridine degradation”. C. López-Alarcón, L.J. Núñez-Vergara, J.A. Squella.

Jul.-1998 XXIII Latinoamerican Congress of Chemistry. Río Grande, Puerto Rico. Lomefloxacin: voltammetric behaviour and electroanalytical applications”, A. Álvarez-Lueje, C. López, L.J. Núñez-Vergara, J.A. Squella.

ORAL PRESENTATIONS

-“Invited speaker” National Meeting & Exposition of the American Chemical Society. Boston, fall-2010.
-Conference at University of Rennes 1, Rennes, France. October, 2014.
-Conference at University of Rennes 1, Rennes, France. June, 2017.
-Conference at University of Greifswald, Germany. June, 2017.
-14th Latino American Conference on Physical Organic Chemistry (CLAFQO-14), May 2017, Concon, Chile.