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Maria Isabel Niemeyer Marich

Research

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

Líneas de Investigación


Estructura funcion de canales ionicos; Sensores de pH; Fisio(pato)logía epitelial

Educación

  •  Licenciatura en Ciencias, mención Biología, UNIVERSIDAD DE CHILE. Chile, 1972
  •  Certificate in Education, Homerton College, University of Cambridge. Reino Unido, 1978
  •  Philosophy, New Hall and Department of Zoology, University of Cambridge. Chile, 1989

Experiencia Académica

  •   Research Assistant Full Time

    University of Cambridge

    Cambridge, Reino Unido

    1981 - 1986

  •   Postdoctoral Fellow Full Time

    MRC Medical Research Council

    Cambridge, Reino Unido

    1989 - 1992

  •   Visiting Scientist Full Time

    UNIVERSIDAD DE CHILE

    Medicina

    Santiago, Chile

    1992 - 1993

  •   Post-doctoral fellow Full Time

    MRC Medical Research Council

    Cambridge, Reino Unido

    1993 - 1994

  •   Assistant professor Other

    UNIVERSIDAD DE SANTIAGO DE CHILE

    Ciencias Biomédicas

    Santiago, Chile

    1994 - 1995

  •   Investigador Asociado Full Time

    UNIVERSIDAD DE SANTIAGO DE CHILE

    Santiago, Chile

    1995 - 1998

  •   Assistant professor Full Time

    UNIVERSIDAD DE CHILE

    Medicina

    Santiago, Chile

    1998 - 2000

Formación de Capital Humano


Undergraduate Students:
* Javier Urra Delgado, "Effects of double mutation Y219F-H220M on the pH sensor of the potassium channel TASK2", Undergraduate Universidad Austral de Chile. Finished 2005.
* Carla Araya Paredes, “Estudio de la interacción de TASK-2 con proteínas renales mediante el ensayo de doble hibrido en membrana”. Undergraduate Medical Technologist. Universidad Austral de Chile. Finished 2011.
* Bárbara Arevalo Ramos, "Modelamiento molecular y análisis del gating por pH0 en el canal de potasio TASK-3". Undergraduate Engineer in Bioinformatics. Universidad de Talca. Finished 2013.


Difusión y Transferencia


Visit abroad
* Collaborative scientific meeting with Dr. Dominique Eladari y Dra. Regine Chambrey, Institut national de la santé et de la recherche médicale, Paris, May 2013.
* Collaborative scientific meeting with Dr. Jacques Teulon, Institut national de la santé et de la recherche médicale, Paris, 19th May to 13th June 2014.
* Collaborative scientific meeting with Dra. Olga Andrini and Dr. Marc Paulais, Universidad Pierre et marie Curie, Paris, 19th May to 13th June 2014.
* Collaborative scientific meeting with Dr. Jacques Teulon, Universidad Pierre et marie Curie, Paris, 09th - 28th July 2015.
* Collaborative scientific meeting with Dr. Jacques Teulon, Universite Pierre et Marie CURIE, Paris, 31th August to 01st October 2016.

Visit in Chile
* Working meetings, Dr. Jacques Teulon, Universite Pierre et Marie Curie-Paris 6, Paris, 25th - 30th November 2013.
* Collaborative scientific meeting with Dr. Peter Brown, University of Manchester School of Medicine. He also gave a seminar entitled "The Choroid Plexuses-What they do?", 05th - 18th November 2016.
* Collaborative scientific meeting with Dr. Jacques Teulon, Universidad Pierre et marie Curie, Paris, 09th - 31th January 2017

NOTA: M.I.Sepúlveda, nombre de casada usado en publicaciones entre el año 1984 al 1994
M.I. Niemeyer usado en su tesis doctoral y en publicaciones posteriores al año 1994.



 

Article (44)

Phosphatidylinositol (4,5)bisphosphate dynamically regulates the K-2P background K+ channel TASK-2
Gating, Regulation, and Structure in K2P K+ Channels: In Varietate Concordia?
Channel-Mediated Lactate Release by K+-Stimulated Astrocytes
Identification and Functional Expression of a Glutamate and Avermectin-Gated Chloride Channel from Caligus rogercresseyi, a Southern Hemisphere Sea Louse Affecting Farmed Fish
Zebrafish and mouse TASK-2 K+ channels are inhibited by increased CO2 and intracellular acidification
An Extracellular Ion Pathway Plays a Central Role in the Cooperative Gating of a K-2P K+ Channel by Extracellular pH
G protein modulation of K-2P potassium channel TASK-2
Gating of a pH-Sensitive K-2P Potassium Channel by an Electrostatic Effect of Basic Sensor Residues on the Selectivity Filter
NBCe1 Mediates the Acute Stimulation of Astrocytic Glycolysis by Extracellular K+
Separate Gating Mechanisms Mediate the Regulation of K-2P Potassium Channel TASK-2 by Intra- and Extracellular pH
Rapid Recycling of ClC-2 Chloride Channels Between Plasma Membrane and Endosomes: Role of a Tyrosine Endocytosis Motif in Surface Retrieval
Voltage-dependent and -independent titration of specific residues accounts for complex gating of a ClC chloride channel by extracellular protons
Neutralization of a single arginine residue gates open a two-pore domain, alkali-activated K+ channel
Gating of two-pore domain K+ channels by extracellular pH
Removal of gating in voltage-dependent ClC-2 chloride channel by point mutations affecting the pore and C-terminus CBS-2 domain
Two splice variants derived from a Drosophila melanogaster candidate ClC gene generate ClC-2-type Cl(-) channels
Basolateral localization of native ClC-2 chloride channels in absorptive intestinal epithelial cells and basolateral sorting encoded by a CBS-2 domain di-leucine motif
Basolateral ClC-2 chloride channels in surface colon epithelium: Regulation by a direct effect of intracellular chloride
Functional evaluation of human ClC-2 chloride channel mutations associated with idiopathic generalized epilepsies
The voltage-dependent CIC-2 chloride channel has a dual gating mechanism
A conserved pore-lining glutamate as a voltage- and chloride-dependent gate in the CIC-2 chloride channel
Extracellular conserved cysteine forms an intersubunit disulphide bridge in the KCNK5 (TASK-2) K+ channel without having an essential effect upon activity
A voltage-independent K+ conductance activated by cell swelling in Ehrlich cells is modulated by a G-protein-mediated process
ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization
Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel
Mutation of the GABAA receptor M1 transmembrane proline increases GABA affinity and reduces barbiturate enhancement.
Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel
K+ conductance activated during regulatory volume decrease. The channels in Ehrlich cells and their possible molecular counterpart
Modulation of the two-pore domain acid-sensitive K+ channel TASK-2 (KCNK5) by changes in cell volume
The role of the agonist binding site in Ca2+ inhibition of the recombinant 5-HT3A receptor
Characterisation of a cell swelling-activated K+-selective conductance of Ehrlich mouse ascites tumour cells
K+ currents activated by leukotriene D-4 or osmotic swelling in Ehrlich ascites tumour cells
Splice variants of a ClC-2 chloride channel with differing functional characteristics
Different efficacy of specific agonists at 5-HT 3 receptor splice variants: the role of the extra six amino acid segment
Voltage control of calcium transients elicited by caffeine and tetracaine in cultured rat muscle cells.
Actions of acromelic acid on nervous system l-glutamate receptors
Chlorpromazine and QX222 block 5-HT3 receptors in N1E-115 neuroblastoma cells.
Characterization of [3H]meta-chlorophenylbiguanide binding to 5-HT3 receptors in N1E-115 neuroblastoma cells.
5-HT3 receptors in NG108-15 neuroblastoma x glioma cells: effect of the novel agonist 1-(m-chlorophenyl)-biguanide.
Pharmacologically separable [(3)H]glutamate binding sites on insect muscle membranes.
The agonist properties of m-chlorophenylbiguanide and 2-methyl-5-hydroxytryptamine on 5-HT3 receptors in N1E-115 neuroblastoma cells.
Molecular target size of an L-glutamate receptor from the cockroach central nervous system.
Pharmacology of an insect central nervous system [(3)H]l-glutamate binding site.
Compartmentalization of sickle-cell calcium in endocytic inside-out vesicles.

ConferencePoster (1)

Phosphatidylinositol (4,5)-bisphosphate dynamically regulates the K2P background K+ channel TASK-2.

ConferenceProceedingWhole (1)

Direct interactions of TASK- 2 potassium channel with components of the HCO3 - transport machinery

EditorialMaterial (2)

B lymphocytes taken to task: a role for a background conductance K-2P K+ channel in B cells. Focus on "Expression of TASK-2 and its upregulation by B cell receptor stimulation in WEHI-231 mouse immature B cells"
ClC-2 channels get new partners. Focus on Association between Hsp90 and the ClC-2 chloride channel upregulates channel function"

Letter (1)

No evidence for a role of CLCN2 variants in idiopathic generalized epilepsy

Proyecto (12)

CANALES DE CLORURO ACTIVADOS POR CALCIO EN EL EPITELIO INTESTINAL COMO MEDIADORES DE LA SECRECION DE FLUIDO: POTENCIALES MEDIADORES DE LA DIARREA ROTAVIRAL Y MODIFICADORES DEL DEFECTO INTESTINAL EN LA FIBROSIS QUISTICA
ESTUDIO CELULAR Y MOLECULAR D LA VIA BASOLATERAL DE SALIDA DE CLORURO EN EL EPITELIO INTESTINAL
EXPLORING THE POSSIBLE ROLE OF THE INWARDLY RECTIFYING K+ CHANNEL KIR7.1 IN THE CILIATED EPITHELIA OF THE CHOROID PLEXUS AND THE CILIARY BODY OF THE EYE
EXTRACELLULAR AND INTRACELLULAR SENSORS IN THE GATING OF TASK-2, A TWO-PORE-DOMAIN POTASSIUM CHANNEL
MODULACION DE LA ACTIVIDAD DEL CANAL DE POTASIO DE DOS POROS EN TANDEM TASK-2 POR SUBUNIDADES DE PROTEINAS G HETEROTRIMERICAS.
MOLECULAR DISSECTION TO STUDY THE CONTRIBUTION OF THE INTRA AND EXTRACELLULAR PH SENSORS OF TASK-2 CHANNELS ON ITS RENAL FUNCTION. AN EXPERIMENTAL APPROACH USING TRANSGENIC MICE TO RESCUE THE TASK-2-NULL PHENOTYPE.
Molecular identification and functional characterisation of the volume regulated epithelial potassium channel.
Necrotic liver cell death: dissection of molecular effector mechanisms.
Participation of TASK-2, a pH-sensitive K+ channel in volume regulation and apoptosis.
Regulation mechanism and oligomeric structure of a chloride channel expressed in epithelia.
REGULATION OF TASK-2 A K2P POTASSIUM CHANNEL, BY PHOSPHOINOSIDES.
Role of an intracellular segment in the modulation of the 5-HT3: receptor structure-function relationship studied by site directed mutagenesis.

Review (4)

MOLECULAR ASPECTS OF STRUCTURE, GATING, AND PHYSIOLOGY OF pH-SENSITIVE BACKGROUND K-2P AND Kir K+-TRANSPORT CHANNELS
TASK-2 K-2P K+ channel: thoughts about gating and its fitness to physiological function
TASK-2: a K-2p K+ channel with complex regulation and diverse physiological functions
TMEM16 proteins: the long awaited calcium-activated chloride channels?
65
Maria Niemeyer

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

34
Francisco Sepúlveda

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

32
Luis Cid

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

10
Leandro Zuñiga

Profesor Asociado

Medicine

Universidad de Talca

Talca, Chile

6
Marcelo Catalan

Profesor Auxiliar

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile

5
Wendy Gonzalez

Director of the Center for Bioinformatics, Simulations and Modeling (CBSM)

Bioinformática

CENTRO DE BIOINFORMÁTICA Y SIMULACIÓN MOLECULAR (CBSM)/ UNIVERSIDAD DE TALCA

Talca, Chile

4
Fernando Gonzalez

Titular Professor

Facultad de Ciencias de la Vida

Universidad Andres Bello

Santiago, Chile

4
Carlos Flores

Researcher

Biology Lab

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

4
Isabel Cornejo

Investigadora

Biología

Centro de Estudios Científicos

Valdivia, Chile

3
Andres Stutzin

Full Professor

ICBM

FACULTAD DE MEDICINA UNIVERSIDAD DE CHILE

Santiago, Chile

3
Luis Barros

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

3
Carlos Figueroa

Head

Anatomy, Histology and Pathology

Universidad Austral de Chile

Valdivia, Chile

2
Diego Varela

Profesor Asociado

Fisiología y Biofísica

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

2
CAROLINA AÑAZCO

Associate Professor

Preclinical Sciences

Universidad Católica del Maule

TALCA, Chile

1
Alejandro San Martin

Senior Reseacher

Biología

CENTRO DE ESTUDIOS CIENTIFICOS

Valdivia, Chile

1
Bárbara Arévalo

Investigador

Investigación

Centro de Estudios de Alimentos Procesados

Talca, Chile

1
VALERIA MARQUEZ

Profesor Asistente

Centro de Nanotecnologia Aplicada

Universidad Mayor de Chile

SANTIAGO, Chile

1
VANESSA MARABOLI

Ingeniera Bioinformática

Facultad de Ciencias de la Vida

Universidad Andrés Bello

Santiago, Chile

1
Gaspar Peña

Profesor Asistente

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile