Man

Carlos Alejandro Flores Pinilla

Researcher

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

Líneas de Investigación


Transepithelial transport; Ion channels; Epithelial physiology and pathophysiology; Cystic fibrosis, Transgenic mice, cell migration and volume regulation.

Educación

  •  Cellular and Molecular Biology, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 2007
  •  Physiology, UNIVERSIDAD DE CONCEPCION. Chile, 2001
  •  Tecnologo Medico, UNIVERSIDAD DE LA FRONTERA. Chile, 1997

Experiencia Académica

  •   Researcher Full Time

    CENTRO DE ESTUDIOS CIENTIFICOS DE SANTIAGO

    Biology Laboratory

    Valdivia, Chile

    2010 - A la fecha

Experiencia Profesional

  •   Researcher Full Time

    Centro de Estudios Cientificos (CECs)

    Valdivia, Chile

    2010 - A la fecha

Formación de Capital Humano


2014
Profesor Patrocinante
"Rol de los Mastocitos en la Inflamación Crónica Alérgica de las Vías Aéreas"
Amber R. Philp
Para optar al título profesional de Bioquímico (en desarrollo)

Profesor Patrocinante
“Caracterización de mastocitos STAT6 knock out”
Rodrigo Gonzalez Kindley.
Para optar a Licenciatura y Grado de Tecnología Médica.
Universidad Austral de Chile.

Profesor Patrocinante
“Subclonamiento del promotor de músculo liso SMP8 para la generación del animal SMP8-TMEM16A+/+/TMEM16A-/-”
José Álvarez Iturra.
Para optar a Licenciatura y Grado de Tecnología Médica.
Universidad Austral de Chile.

Profesor Patrocinante
“Rol de KCNN4 en neutrófilos”
Daniel Vera Díaz.
Para optar a Licenciatura y Grado de Tecnología Médica.
Universidad Austral de Chile.

2013
Profesor Patrocinante
“Generación del animal SMP8-TMEM16A+/+/TMEM16A-/- y estudio de la distribución del canal TMEM16A en tejidos de ratón.”
José Arellano Ruiz.
Para optar a Licenciatura y Grado de Tecnología Médica.
Universidad Austral de Chile.

Profesor Patrocinante
“Inactivación de genes involucrados en enfermedades humanas del epitelio intestinal.”
Pamela Millar Büchner.
Para optar a Licenciatura en Biología.
Universidad Austral de Chile.

Profesor Patrocinante
“Regulación de mastocitos en la fibrosis quística por el canal KCNN4.”
Texia Riquelme Mora.
Para optar a Licenciatura y Grado de Tecnología Médica.
Universidad Austral de Chile.


Premios y Distinciones

  •   Best Poster Presentation

    European Cystic Fibrosis Society

    España, 2013

    Best Poster Presentation


 

Article (23)

Lack of Kcnn4 improves mucociliary clearance in muco-obstructive lung disease.
Monocarboxylate transporter 4 (MCT4) is a high affinity transporter capable of exporting lactate in high-lactate microenvironments
Normal Calcium-Activated Anion Secretion in a Mouse Selectively Lacking TMEM16A in Intestinal Epithelium
Kcnn4 is a modifier gene of intestinal cystic fibrosis preventing lethality in the Cftr-F508del mouse
Delphinidin Reduces Glucose Uptake in Mice Jejunal Tissue and Human Intestinal Cells Lines through FFA1/GPR40
ClC-2 and intestinal chloride secretion
Pharmacological analysis of epithelial chloride secretion mechanisms in adult murine airways
Severe changes in colon epithelium in the Mecp2-null mouse model of Rett syndrome
The calcium-activated potassium channel KCa3.1 plays a central role in the chemotactic response of mammalian neutrophils
Delphinol® standardized maqui berry extract reduces postprandial blood glucose increase in individuals with impaired glucose regulation by novel mechanism of sodium glucose cotransporter inhibition
Disruption of CDH2/N-cadherin-based adherens junctions leads to apoptosis of ependymal cells and denudation of brain ventricular walls
Severe Defects in Absorptive Ion Transport in Distal Colons of Mice That Lack ClC-2 Channels
Strain-dependent differences in electrogenic secretion of electrolytes across mouse colon epithelium
Abolition of Ca 2+-mediated intestinal anion secretion and increased stool dehydration in mice lacking the intermediate conductance Ca 2+-dependent K + channel Kcnn4
Abolition of Ca2+-mediated intestinal anion secretion and increased stool dehydration in mice lacking the intermediate conductance Ca2+-dependent K+ channel Kcnn4
Two splice variants derived from a Drosophila melanogaster candidate ClC gene generate ClC-2-type Cl(-) channels
Functional evaluation of human ClC-2 chloride channel mutations associated with idiopathic generalized epilepsies
Cell signalling-mediating insulin increase of mRNA expression for cationic amino acid transporters-1 and-2 and membrane hyperpolarization in human umbilical vein endothelial cells
Rapid stimulation of L-arginine transport by D-glucose involves P42/44(mapk) and nitric oxide in human umbilical vein endothelium
Inhibition of nitrobenzylthioinosine-sensitive adenosine transport by elevated D-glucose involves activation of P-2Y2 purinoceptors in human umbilical vein endothelial cells
Modulation of adenosine transport by insulin in human umbilical artery smooth muscle cells from normal or gestational diabetic pregnancies
Regulation of adenosine transport by D-glucose in human fetal endothelial cells: involvement of nitric oxide, protein kinase C and mitogen-activated protein kinase
Complementary studies to histologic diagnosis in surgical pathology

Abstract (4)

Diabetes and hyperglicemia induced L-arginine/nitric oxide (NO) pathway in human fetal endothelium
Elevated D-glucose induces expression of the cationic amino acid transport system y(+)/CAT-2B and endothelial nitric oxide synthase in human fetal endothelial cells
Diabetes- and insulin-increased adenosine transport is associated with a higher number of nucleoside transporters in human umbilical artery smooth muscle cells
Differential role of protein kinase C in D-glucose-induced inhibition of L-leucine and thymidine incorporation into macromolecules in human fetal endothelium

BookSection (1)

Monitoring Lactate Dynamics in Individual Macrophages with a Genetically Encoded Probe

EditorialMaterial (2)

B lymphocytes taken to task: a role for a background conductance K-2P K+ channel in B cells. Focus on "Expression of TASK-2 and its upregulation by B cell receptor stimulation in WEHI-231 mouse immature B cells"
A tale from the Crypt: splice variants of BK channels in colonic potassium secretion

Errata (1)

Normal Calcium-Activated Anion Secretion in a Mouse Selectively Lacking TMEM16A in Intestinal Epithelium (vol 10, 694, 2019)

Proyecto (4)

ROLE OF KCNN4 IN THE TRANSEPITHELIAL TRANSPORT OF ELECTROLYTES IN THE AIRWAYS. IMPLICATIONS IN HEALTH AND OBSTRUCTIVE AIRWAY DISEASES.
IDENTIFICATION OF POTASSIUM CHANNELS INVOLVED IN SODIUM-COUPLED ABSORPTION NUTRIENTS AND IN THE SECRETION OF ELECTROLYTES AND FLUID IN INTESTINAL EPITHELIUM.
KCNN4 AS A MODIFIER GENE OF INTESTINAL CYSTIC FIBROSIS SEVERITY. POSSIBLE ROLE OF MAST CELLS.
MODIFICACION DEL FENOTIPO INTESTINAL EN LA FIBROSIS QUISTICA POR EL CANAL DE POTASIO KCNN4 Y EL BACKGROUND GENETICO EN RATONES.

Review (2)

TMEM16 proteins: the long awaited calcium-activated chloride channels?
Tumorogenesis and mdm2 protein
36
Carlos Flores

Researcher

Biology Lab

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

7
Francisco Sepúlveda

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

6
Luis Cid

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

6
LUIS SOBREVIA

Professor

Division of Obstetrics and Gynaecology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

4
Claudio Aguayo

Profesor Asociado

Bioquimica Clinica e Inmunologia

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

3
Anita Guequen

Asistente de investigación

Biología

Centro Estudios Científicos (CECs)

Valdivia, Chile

3
Maria Niemeyer

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

3
Paola Casanello

Profesor Asociado

División de Obstetricia y Ginecología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Viviana Montecinos

Assistant Professor

Hematology Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
INGRID EHRENFELD

ASSOCIATE PROFESSOR

ANATOMIA; HISTOLOGIA & PATOLOGIA

UNIVERSIDAD AUSTRAL DE CHILE

VALDIVIA, Chile

1
Jorge Hidalgo

Profesor Auxiliar

Instituto de Medicina

Universidad Austral de Chile

Valdivia, Chile

1
Rafael Burgos

FULL PROFESSOR

PHARMACOLOGY AND MORPHOPHYSIOLOGY

UNIVERSIDAD AUSTRAL DE CHILE

VALDIVIA, Chile

1
Isabel Cornejo

Investigadora

Biología

Centro de Estudios Científicos

Valdivia, Chile

1
Jose Sarmiento

Assistant Professor

Physiology

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Pamela Sandoval

Postdoctoral Researcher

Biología

Centro de Estudios Científicos -CECs-

Valdivia, Chile

1
Claudio Henríquez

Auxiliar Professor

Farmacology and Morphophysiology

Universidad Austral de Chile

Valdivia, Chile

1
Carlos Figueroa

Head

Anatomy, Histology and Pathology

Universidad Austral de Chile

Valdivia, Chile

1
César González

Profesor Asistente

Facultad de Medicina y Ciencias

Universidad San Sebastián

Puerto Montt, Chile

1
Luis Federico Batiz

Associate Professor

Center for Biomedical Research and Innovation (CIIB)

Universidad de los Andes

Santiago, Chile

1
MARIA HIDALGO

Associate professor

Pharmacology

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Alejandro San Martin

Senior Reseacher

Biología

CENTRO DE ESTUDIOS CIENTIFICOS

Valdivia, Chile

1
Genesis Vega

Investigador

Centro de Biología Integrativa

Universidad Mayor de Chile

Santiago, Chile

1
Marcelo Catalan

Profesor Auxiliar

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile