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Luis Pablo Cid Soto

Principal Research

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

Líneas de Investigación


Canales de cloruro y potasio de epitelios; Estructura -funcion de canales ionicos; Renal and Intestinal physiology and pathophysiology.

Educación

  •  Medical Doctor, UNIVERSIDAD DE CHILE. Chile, 1982
  •  Physiologic and Genetics, Johns Hopkins University. Estados Unidos, 1995
  •  Magister in Cs. Physicians Mention Pathophysiology, UNIVERSIDAD DE CHILE. Chile, 2000

Experiencia Académica

  •   Instructor Full Time

    UNIVERSIDAD DE CHILE

    Faculty of Medicine

    Santiago, Chile

    1988 - 1991

  •   Postdoctoral Fellow Full Time

    Johns Hopkins University

    Baltimore, Estados Unidos

    1991 - 1995

  •   Instructor Full Time

    UNIVERSIDAD DE CHILE

    Faculty of Medicine

    Santiago, Chile

    1995 - 2000

Experiencia Profesional

  •   Medico Full Time

    .

    Lautaro, Chile

    1983 - 1987

Formación de Capital Humano


Undergraduate Students:

* Juan Pablo Donoso. “Study of interaction of TASK-2 with proteins involved in renal bicarbonate reabsorption: Carbonic Anhydrase II and Sodium Bicarbonate Cotransporter”. Undergraduate Medical Technologist. Universidad Austral de Chile. Finished 2014.
* Karen López C. "Expression of the potassium channel Kir 7.1 and NHE3 sodium proton exchanger in TASK2 KO mice". Undergraduate Biochemist. Universidad Austral de Chile. Finished in 2013.
* Sandra Villanueva S. “Effect of the genetic inactivation of the KCNQ1/KCNE3 potassium channel in the intestinal chloride secretion using murine models”. Undergraduate Biochemist Universidad Austral de Chile. Finished in 2013.
* Alexandra Alvarez A. “Generation of a transgenic mouse expressing a sensor of extracellular in the basolateral membrane of in the renal proximal tubule”. Undergraduate Biochemist Universidad Austral de Chile. Finished in 2011.
* Moisés Sandoval C. Generation of a genetically coded extracellular pH sensor to measure pH in basolateral epithelia microdomains. Undergraduate Biochemist Universidad Austral de Chile. Finished in 2009.
* Andrea Olave E. “Subcellular localization of ClC-2 chloride channel mutants using IFI”. Undergraduate Medical Technologist. Universidad Austral de Chile. Finished in 2009.
* Johanna Burgos V. “Study of the inhibitory mechanism by extracellular acid pH of the chloride channel ClC-Kb”. Undergraduate Biochemist Universidad Austral de Chile. Finished in 2007.
* Carola Maturana F. "Study of the interaction of the SPAK protein kinase with the ClC-2 chloride channel". Undergraduate Biochemist Universidad Austral de Chile. Finished in 2007.
* Gaspar Peña M. "Localization basolateral of the ClC-2 echoride channel in epithelial cell lines". Undergraduate Biochemist Universidad Austral de Chile. Finished 2005.
* Yamil Yusef R. "Effect of an intronic mutation in the function of the ClC-2 choride channel". Undergraduate of the Marine Biology. Universidad Austral de Chile. Finished in 2004.
* Leandro Zúñiga H. "Study of the mechanism of the ClC-2 channel opening by site directed mutagenesis of cysteine 256". Undergraduate Biochemist Universidad Austral de Chile. Finished 2002.
* Isabel Cornejo A. "Cloning and expression of the chloride channel ClC-5 from intestine epithelia of guinea pig".Undergraduate Medical Technologist. Universidad Austral de Chile. Finished in 2000.
* Felipe Estrada A. Generation of knock-in mice with a double lysine mutation in TASK-2 potassium channel using CRISPR-Cas9 technology. Undergraduate Medical Technologist. Universidad Austral de Chile. Ongoing.

Master Student:
Hugo Roa R. "Phenotypic rescue of the KO TASK-2 mouse by expression of the pH sensors mutants of the channel in the renal proximal tubule". Magister in Cellular Biology. Universidad Austral de Chile. Finished 2016.

PhD Student:
* Isabel Cornejo A. "Study of the traffic and membrane surface expression of the ClC-2 chloride channel. Cellular and Molecular Biology PhD. Universidad Austral de Chile. Finished in 2007.


Difusión y Transferencia


Visit abroad
* Karen Lopez visit the Institut national de la santé et de recherche médicale (Inserm) in Paris and work in the laboratory of Dr. Jacques Teulon, learning the technique of perfusion via the aorta artery, the preparation of samples for the immunofluorescence of kidney, protein quantification and westernblot of renal proteins, 01st May to 01st June 2013.
* Karen Lopez visit the Hôpital Européen Georges-Pompidou in Paris and work in the laboratory of Dr. Dominique Eladari, to perform some analysis of samples, 01st May to 01st June 2013.

Visit in Chile
* Working meetings, Dr. Dominique Eladari and Dra. Regine Chambrey, Université de Paris Decartes Paris, 19th to 31th December 2016.

Collaborative Activities
* Course Program Modules Scholarship Nephrology, "Use of genetically modified animals in the study of physiology and renal disease", Chilean Society of Nephrology, 22- 25 april 2015, Santiago, Chile.
* Course Science and Poetry, "A surprising immune system in bacteria gives us tools to modify complex genomes", Universidad Austral de Chile, 10 May 2016, Valdivia, Chile.

National collaborators
* Leopoldo Ardiles (Universidad Austral de Chile, Valdivia).
* Sandra Bravo (Universidad Austral de Chile, Puerto Montt).
* Marcelo Catalán (Universidad Arturo Prat, Iquique).
* Wendy González (Universidad de Talca, Talca).
* Danilo González-Nilo (Universidad Andrés Bello, Santiago).

International collaborators
* Jacques Teulon and Marc Paulais (Université Pierre at Marie Curie, France),
* Olga Andrini (Université Claude Bernard, France),
* Brian Harvey (Royal College of Surgeons, Dublin Ireland),
* Sarah C. Lummis (University of Cambridge, UK),
* David M. Valenzuela and Venus Lai (Regeneron Pharmaceuticals, NY, USA),
* Dominique Eladari and Régine Chambrey (Université de La Réunion, France.),
* Thomas Jentsch (Leibniz Molecular Pharmacology Institute, Berlin Germany).



 

Article (49)

Altered phosphatidylinositol regulation of mutant inwardly rectifying K+ Kir7.1 channels associated with inherited retinal degeneration disease.
Short Chain Fatty Acids Effect on Chloride Channel ClC-2 as a Possible Mechanism for Lubiprostone Intestinal Action
A novel Kir7.1 splice variant expressed in various mouse tissues shares organisational and functional properties with human Leber amaurosis-causing mutations of this K+ channel.
Kir7.1 inwardly rectifying K+ channel is expressed in ciliary body non pigment epithelial cells and might contribute to intraocular pressure regulation.
K-2P TASK-2 and KCNQ1-KCNE3 K+ channels are major players contributing to intestinal anion and fluid secretion
Kcnn4 is a modifier gene of intestinal cystic fibrosis preventing lethality in the Cftr-F508del mouse.
Reply from L. P. Cid, T. J. Jentsch and F. V. Sepulveda: intestinal electrolyte and fluid secretion - a model in trouble?
Tissue Distribution of Kir7.1 Inwardly Rectifying K+ Channel Probed in a Knock-in Mouse Expressing a Haemagglutinin-Tagged Protein
Phosphatidylinositol (4,5)bisphosphate dynamically regulates the K-2P background K+ channel TASK-2
Gating, Regulation, and Structure in K2P K+ Channels: In Varietate Concordia?
Cleft Palate, Moderate Lung Developmental Retardation and Early Postnatal Lethality in Mice Deficient in the Kir7.1 Inwardly Rectifying K+ Channel
Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.
Identification and functional expression of a glutamate- and avermectin-gated chloride channel from Caligus rogercresseyi, a southern Hemisphere sea louse affecting farmed fish.
Zebrafish and mouse TASK-2 K+ channels are inhibited by increased CO2 and intracellular acidification
An Extracellular Ion Pathway Plays a Central Role in the Cooperative Gating of a K-2P K+ Channel by Extracellular pH
G protein modulation of K-2P potassium channel TASK-2
G protein modulation of K_2P potassium channel TASK-2: a role of basic residues in the C-terminus domain.
Novel CLCNKB Mutations Causing Bartter Syndrome Affect Channel Surface Expression
Extracellular pH in Restricted Domains as a Gating Signal for Ion Channels Involved in Transepithelial Transport
Gating of a pH-Sensitive K-2P Potassium Channel by an Electrostatic Effect of Basic Sensor Residues on the Selectivity Filter
Sexual dimorphism and oestrogen regulation of KCNE3 expression modulates the functional properties of KCNQ1 K+ channels
Separate Gating Mechanisms Mediate the Regulation of K-2P Potassium Channel TASK-2 by Intra- and Extracellular pH
Strain-dependent differences in electrogenic secretion of electrolytes across mouse colon epithelium
Rapid Recycling of ClC-2 Chloride Channels Between Plasma Membrane and Endosomes: Role of a Tyrosine Endocytosis Motif in Surface Retrieval
Voltage-dependent and -independent titration of specific residues accounts for complex gating of a ClC chloride channel by extracellular protons
A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells
Neutralization of a single arginine residue gates open a two-pore domain, alkali-activated K+ channel
Gating of two-pore domain K+ channels by extracellular pH
Hereditary parkinsonism with dementia is caused by mutations in ATP13A2, encoding a lysosomal type 5 P-type ATPase
Hereditary parkinsonism with dementia is caused by mutations in ATP13A2, encoding a lysosomal type 5 P-type ATPase.
Removal of gating in voltage-dependent ClC-2 chloride channel by point mutations affecting the pore and C-terminus CBS-2 domain
Two splice variants derived from a Drosophila melanogaster candidate ClC gene generate ClC-2-type Cl(-) channels
Basolateral localization of native ClC-2 chloride channels in absorptive intestinal epithelial cells and basolateral sorting encoded by a CBS-2 domain di-leucine motif
Functional evaluation of human ClC-2 chloride channel mutations associated with idiopathic generalized epilepsies
Putative ClC-2 chloride channel mediates inward rectification in Drosophila retinal photoreceptors
The voltage-dependent CIC-2 chloride channel has a dual gating mechanism
A conserved pore-lining glutamate as a voltage- and chloride-dependent gate in the CIC-2 chloride channel
Extracellular conserved cysteine forms an intersubunit disulphide bridge in the KCNK5 (TASK-2) K+ channel without having an essential effect upon activity
ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization
Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel
Aquaporin-2, a regulated water channel, is expressed in apical membranes of rat distal colon epithelium
Cloning, cellular distribution and functional expression of small intestinal epithelium guinea pig C1C-5 chloride channel
K+ conductance activated during regulatory volume decrease. The channels in Ehrlich cells and their possible molecular counterpart
Modulation of the two-pore domain acid-sensitive K+ channel TASK-2 (KCNK5) by changes in cell volume
Splice variants of a ClC-2 chloride channel with differing functional characteristics
Separate taurine and chloride efflux pathways activated during regulatory volume decrease
Analysis of ClC-2 channels as an alternative pathway for chloride conduction in cystic fibrosis airway cells.
Modulation by extracellular Cl- of volume-activated organic osmolyte and halide permeabilities in HeLa cells.
Cloning of a putative human voltage - gated chloride channel (CIC-2) cDNA widely expressed in human tissues

Abstract (6)

Differences in electrogenic secretion of electrolytes in colonic epithelium of inbred mouse strains.
Basolateral ClC-2 chloride channels in surface colon epithelium: Regulation by a direct effect of intracellular chloride
Effect of mutation C256A in rat ClC-2 on its gating behavior
Expression of aquaporin-2 in rat distal colon: Effect of water restriction
Characterisation of ClC-2 chloride channel splice variants
Expression of aquaporin-2 in the rat colon: effect of thirsting

ConferencePoster (1)

"Indirect identi fi cation of the TASK2 potassium channel expression by β -galactosidase activity"

ConferenceProceedingWhole (1)

Direct G protein modulation of K2P channel TASK-2: a role for basic residues in the C-terminus domain

EditorialMaterial (3)

B lymphocytes taken to task: a role for a background conductance K-2P K+ channel in B cells. Focus on "Expression of TASK-2 and its upregulation by B cell receptor stimulation in WEHI-231 mouse immature B cells"
A tale from the Crypt: splice variants of BK channels in colonic potassium secretion
ClC-2 channels get new partners. Focus on Association between Hsp90 and the ClC-2 chloride channel upregulates channel function"

Letter (1)

No evidence for a role of CLCN2 variants in idiopathic generalized epilepsy

Proyecto (37)

EXPLORING THE POSSIBLE ROLE OF THE INWARDLY RECTIFYING K+ CHANNEL KIR7.1 IN THE CILIATED EPITHELIA OF THE CHOROID PLEXUS AND THE CILIARY BODY OF THE EYE
CHARACTERIZATION OF THE ROLE OF KIR 7.1 POTASSIUM CHANNELS IN RENAL ELECTROLYTE TRANSPORT USING A GENETIC APPROACH.
REGULATION OF TASK-2 A K2P POTASSIUM CHANNEL, BY PHOSPHOINOSIDES.
-Identificación of potassium channels involved in sodium-coupled absorption nutrients and in the secretion of electrolytes and fluid in intestinal epithelium
Molecular dissection to study the contribution of the intra and extracellular pH sensors of TASK-2 channels on its renal function. An experimental approach using transgenic mice to rescue the TASK-2-null phenotype
MOLECULAR DISSECTION TO STUDY THE CONTRIBUTION OF THE INTRA AND EXTRACELLULAR PH SENSORS OF TASK-2 CHANNELS ON ITS RENAL FUNCTION. AN EXPERIMENTAL APPROACH USING TRANSGENIC MICE TO RESCUE THE TASK-2-NULL PHENOTYPE.
Molecular dissection to study the contribution of the intra and extracellular pH sensors of TASK-2 channels on its renal function. An experimental approach using transgenic mice to rescue the TASK-2-null phenotype”.
-Mecanismos moleculares de regulacion del canal de potasio KCNQ1/KCNE3 por 17 beta-estradiol.
Extracellular and intracellular sensors in the gating of TASK-2, a two-pore-domain potassium channel.
Microdominios tisulares de pH extracelular estudiados in situ con un sensor codificado genéticamente en ratones.
Tissue pH microdomains of extracellular pH studied in situ with a genetically coded sensor in mice
Mecanismos moleculares del efecto dual de los protones extracelulares sobre el gating del canal de cloruro ClC-2.
Canales de cloruro activados por calcio en el epitelio intestinal como mediadores de la secreción de fluido=> potenciales mediadores de la diarrea rotaviral y modificadores del defecto intestinal en la fibrosis quística .
- Generación de un ratón que exprese el canal ClC -2 unido a la proteína fluorescente verde. Estudio de localización e implicaciones funcionales.
Es posible revertir el sindrome de Rett? restitución condicional de mecp2 en modelos murinos para esta enfermedad. 2005-2007. Fondecyt 1051079
Generation of a mouse expressing the ClC-2 chloride channel coupled to the green fluorescent protein. Localization studies and functional implications
Estudio celular y molecular d la vía basolateral de salida de cloruro en el epitelio intestinal.
Epilepsia idiopática generalizada=> estudio funcional de mutaciones en el gen CLCN2.
Idiopathic Generalized Epilepsy: Functional studies of CLC-2 gene mutations
Mecanismo de regulación y estructura oligomérica de un canal de cloruro expresado en epitelios. 2000-2002. Fondecyt 1000622
Papel de los canales epiteliales de la familia ClC en la función intestinal.
Role of the epithelium ClC family chloride channels in the intestinal function
Identificación molecular y caracterización funcional del canal de K+ epitelial activado por aumento del volumen celular. 1998-2001. Fondecyt 1980718
Clonamiento y expresión funcional de canales de cloruro ClC del epitelio intestinal.
Cloning and functional expression of intestinal epithelium ClC family chloride channels
Cellular and molecular studies of the basolateral via of chloride efflux in the intestinal epithelia.
Characterization of the role of Kir 7.1 potassium channels in renal electrolyte transport using a genetic approach.
Chloride channels activated by calcium in the intestinal epithelia as mediators of the fluid secretion: potential mediators of the rotavirus diarrhea and modifiers of the cystic fibrosis intestinal defect
Exploring the possible role of the inwardly rectifying K+ channel Kir7.1 in the ciliated epithelia of the choroid plexus and the ciliary body of the eye.
Extracellular and intracellular sensors in the gating of TASK-2, two-pore-domain potassium channel.
Identificación of potassium channels involved in sodium-coupled absorption nutrients and in the secretion of electrolytes and fluid in intestinal epithelium
Is possible revert the Rett syndrome? conditional restitution of mecp2 gene in murine models of the disease
Mechanism de regulation and oligomeric structure of a chloride channel expressed in epithelia
Molecular identification and molecular characterization of a potassium channel activated by cell swelling
Molecular mechanism of regulation of KCNQ1/KCNE3 potassium channel by 17beta-oestradiol.
Molecular mechanisms of the dual effect of the extracellular protons in the chloride channel ClC-2 gating.
Regulation of TASK-2, a K2P potassium channel, by phosphoinosides.

Review (4)

MOLECULAR ASPECTS OF STRUCTURE, GATING, AND PHYSIOLOGY OF pH-SENSITIVE BACKGROUND K-2P AND Kir K+-TRANSPORT CHANNELS
TASK-2 K-2P K+ channel: thoughts about gating and its fitness to physiological function
TASK-2: a K-2p K+ channel with complex regulation and diverse physiological functions
TMEM16 proteins: the long awaited calcium-activated chloride channels?
79
Luis Cid

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

37
Francisco Sepúlveda

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

30
Maria Niemeyer

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

11
Leandro Zuñiga

Profesor Asociado

Medicine

Universidad de Talca

Talca, Chile

8
Carlos Flores

Researcher

Biology Lab

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

7
Marcelo Catalan

Profesor Auxiliar

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile

6
Isabel Cornejo

Investigadora

Biología

Centro de Estudios Científicos

Valdivia, Chile

5
Wendy Gonzalez

Director of the Center for Bioinformatics, Simulations and Modeling (CBSM)

Bioinformática

CENTRO DE BIOINFORMÁTICA Y SIMULACIÓN MOLECULAR (CBSM)/ UNIVERSIDAD DE TALCA

Talca, Chile

4
Johanna Burgos

Postdoctoral

Biología

Centro de Estudios Científicos

Valdivia, Chile

3
Diego Varela

Profesor Asociado

Fisiología y Biofísica

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

3
Fernando Gonzalez

Titular Professor

Facultad de Ciencias de la Vida

Universidad Andres Bello

Santiago, Chile

3
Sandra Villanueva

Tesista

Biología

Centro de Estudios Científicos

Valdivia, Chile

3
Carlos Figueroa

Head

Anatomy, Histology and Pathology

Universidad Austral de Chile

Valdivia, Chile

2
Moisés Sandoval

Co-Investigador

Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

2
Carlos Vio

Profesor

Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Luis Barros

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

1
Viviana Bustos

Researcher

Universidad de Los Lagos

Valdivia, Chile

1
CAROLINA AÑAZCO

Associate Professor

Preclinical Sciences

Universidad Católica del Maule

TALCA, Chile

1
Bárbara Arévalo

Investigador

Investigación

Centro de Estudios de Alimentos Procesados

Talca, Chile

1
Maria Behrens

Profesor Titular

Neurología y Neurocirugía, y de Neurociencia

Hospital Clínico Universidad de Chile

Santiago, Chile

1
Neudo Buelvas

Asistente de Investigación

Biología

Centro de Estudios Científicos

Valdivia, Chile

1
Rodrigo Alzamora

ASSISTANT PROFESSOR

INSTITUTE OF BIOMEDICAL SCIENCES

UNIVERSIDAD DE CHILE

Santiago, Chile

1
VALERIA MARQUEZ

Profesor Asistente

Centro de Nanotecnologia Aplicada

Universidad Mayor de Chile

SANTIAGO, Chile

1
Dulce Figueiras

Investigador responsable

Medicina

Universidad de La Frontera

Temuco, Chile

1
Gaspar Peña

Profesor Asistente

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

1
Cecilia Vergara

Profesor Asociado

Biología

DEPARTAMENTO BIOLOGIA, FACULTAD DE CIENCIAS, U. DE CHILE

Santiago, Chile

1
Ricardo Delgado

Profesor Adjunto

Biología

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE BIOLOGIA

Santiago, Chile

1
Gonzalo Ugarte

Asistente de Investigación

Biología

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile