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Luis Felipe Barros Olmedo

Principal Research

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

Líneas de Investigación


Brain Energy Metabolism; Metabolic Imaging; Membrane Transport; Cancer Metabolism

Educación

  •  Sciences, UNIVERSIDAD DE CHILE. Chile, 1993
  •  Medico-cirujano, UNIVERSIDAD DE CHILE. Chile, 1988

Experiencia Académica

  •   Profesor Asistente Full Time

    UNIVERSIDAD DE CHILE

    Medicina

    Santiago, Chile

    1996 - 2000

  •   Profesor Asociado Full Time

    UNIVERSIDAD DE CHILE

    Medicina

    Santiago, Chile

    2000 - 2000

  •   Investigador Full Time

    CENTRO DE ESTUDIOS CIENTIFICOS DE SANTIAGO

    Valdivia, Chile

    2000 - A la fecha

Formación de Capital Humano


Participación en Docencia de postgrado en la Universidad Austral de Chile, Universidad de Chile y Universidad Católica de Chile.

TESIS DE DOCTORADO TERMINADAS
Ocupación Actual
Omar Porras Profesor Asistente, INTA, Universidad de Chile.
Carlos Flores Investigador Postdoctoral, Pontificia Universidad Católica de Chile.
Anitsi Loaiza Investigadora Postdoctoral, Universidad Austral de Chile.
Carla Bittner Profesora Asistente, Universidad de Los Lagos.
Iván Ruminot Investigador Postdoctoral, TU Kaiserslautern, Alemania.
Alejandro San Martín Investigador Junior, CECs
Rodrigo Lerchundi Investigador Postdoctoral, University of Dusseldorf, Alemania
Tamara Sotelo Investigadora Postdoctoral, University of Warwick, Inglaterra.

Títulos de tesis
1. Omar Porras, “Efectos del neurotransmisor glutamato sobre la captación de glucosa en neuronas y astrocitos en cultivo”, PhD en Ciencias, Facultad de Medicina, Universidad de Chile, 2006.
2. Carlos Flores,"Acidificación intracelular y su participación en la sobrecarga de calcio por estrés metabólico", PhD en Ciencias, Universidad Austral de Chile, 2008.
3. Carla Bittner, “Acute modulation of astrocyte glucose metabolism by neuronal signals”, PhD en Ciencias, Universidad Austral de Chile, 2009. Premio Mejor Tesis de Doctorado, Sociedad de Biología Celular de Chile 2011.
4. Anitsi Loaiza, "Permeabilidad y metabolismo de la glucosa en astrocitos in situ", PhD en Ciencias, Universidad Austral de Chile, 2010.
5. Iván Ruminot, "Role of the sodium/bicarbonate cotransporter NBCe1 on the stimulation of astrocytic glycolysis by K+", PhD, Universidad Austral de Chile, 2012.
6. Alejandro San Martín, “Nuevos métodos ópticos para el estudio del metabolismo del cáncer y acoplamiento neuro-metabólico a una alta resolución espacio-temporal”, PhD, Universidad Austral de Chile, 2013.
7. Rodrigo Lerchundi, “Acute modulation of astrocytic energy metabolism by NH4+”, PhD, Universidad Austral de Chile, 2015.
8. Tamara Sotelo, “Estudio sobre la liberación de lactato astrocitario por aumento del lactato extracelular”, PhD, Universidad Austral de Chile, 2015.

TESIS DE DOCTORADO EN CURSO
Robin Gutiérrez UACH Redacción de tesis
Nicolás Martínez Universidad Católica de Chile, redacción de tesis (Co-tutoría con el Dr. Felipe Court)
Ignacio Fernández-Moncada UACH tesis en progreso
Robinson Arce UACH tesis en progreso
Felipe Baeza-Lehnert UACH tesis en progreso

TESIS DE PREGRADO TERMINADAS
Rodrigo Quintanilla Universidad de Chile
Omar Porras Universidad de Chile
Anitsi Loaiza UACH, Premio Mejor Tesis de Pregrado, Sociedad de Biología Celular de Chile 2004.
Carla Bittner UACH
Iván Ruminot UACH
Carolina Montenegro UACH
Astrid Schmied UACH
Hans Moldenhauer UACH
Valeria Larenas UACH
Tamara Sotelo UACH
Ignacio Fernández Pontificia Universidad Católica de Valparaiso
Sebastián Ceballo UACH
Rocío Valdebenito UACH
Felipe Baeza-Lehnert UACH
Daniela Espinoza UACH
Yasna Contreras UACH
Pamela Garrido UACH
Esteban Diaz UACH
Francisca Cortés Universidad de Concepción
Ignacio Soto UACH
Daniel Robles UACH

TESIS DE PREGRADO EN CURSO
María Belen Leyton UACH
Héctor Oyarzún UACH


Difusión y Transferencia


Membership
Member of Study Group Medicine, Fondecyt. 2003-2007
Visiting Professorship. Pontificia Universidad Católica de Chile, Santiago, Chile. 2004, 2008, 2010, 2014, 2016.
Member of the High Council for Science and Technology, FONDECYT, Chile. 2007-2010.
President of the Chilean Society for Cell Biology. 2009-2010.

Collaborative Activities
* Course Ciencia y Poesía, "Que es la Vida", Universidad Austral de Chile, 30th May 2017, Valdivia, Chile
* Charla inaugural Doctorado en Ciencias, "Combustion lenta: del flogisto a Warburg y al fMRI", 04th May 2017, University of Concepción.
* Collaborative scientific meeting with, Dr. Bruno Weber, Institute of Pharmacology & Toxicology, University of Zurich, Switzerland
* Collaborative scientific meeting with, Dr. Felipe Court, Director, Center for Integrative Biology Universidad Mayor, Santiago-Chile.
* Collaborative scientific meeting with, Dra. Jimena Sierralta, ICBM School of Medicine, University of Chile, Santiago-Chile.


Premios y Distinciones

  •   Best Medical Graduate

    UNIVERSIDAD DE CHILE

    Chile, 1988

    .

  •   CONICYT Fellowship.

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 1988

    .

  •   British Council Travel Fellowship

    University de Kent at Canterbury and King's College London.

    Reino Unido, 1989

    .

  •   Wellcome Trust International Fellowship

    WELLCOME TRUST

    Chile, 1993

    Head of laboratory, S.A. Baldwin.

  •   Fundación Andes Fellowship

    FUNDACION ANDES

    Chile, 1996

    .

  •   Visiting Professorship

    National Institutes for Physiological Sciences, Okazaki

    Japón, 2001

    .

  •   Outstanding Young Scientist Award

    Society for Biology

    Chile, 2002

    Bios-Chile.

  •   Frontiers in Research Program Investigator.

    Chilean Academy of Sciences.

    Chile, 2004

    .

  •   Visiting Professorship

    TU Kaiserslautern

    Alemania, 2007

    .

  •   Los Rios Region Medal, Science.

    Consejo Regional de Los Rios

    Chile, 2013

    Aporte, creación y desarrollo de la Región de Los Rios en el ámbito de la Ciencia


 

Article (80)

Non-Canonical Control of Neuronal Energy Status by the Na+ Pump
Chaski, a novel Drosophila lactate/pyruvate transporter required in glia cells for survival under nutritional stress
CROSSTALK CrossTalk proposal: an important astrocyte-to-neuron lactate shuttle couples neuronal activity to glucose utilisation in the brain
Glia in brain energy metabolism: A perspective
Modulation of Mammary Stromal Cell Lactate Dynamics by Ambient Glucose and Epithelial Factors
Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism
Near-Critical GLUT1 and Neurodegeneration
Activation of Wnt Signaling in Cortical Neurons Enhances Glucose Utilization through Glycolysis
In Vivo Evidence for a Lactate Gradient from Astrocytes to Neurons
Sodium signaling and astrocyte energy metabolism
Targeting of astrocytic glucose metabolism by beta-hydroxybutyrate
Wnt5a Increases the Glycolytic Rate and the Activity of the Pentose Phosphate Pathway in Cortical Neurons
Channel-Mediated Lactate Release by K+-Stimulated Astrocytes
Channel-mediated lactate release by K+-stimulated astrocytes.
Enhanced Neuronal Glucose Transporter Expression Reveals Metabolic Choice in a HD Drosophila Model
Functional interaction between bicarbonate transporters and carbonic anhydrase modulates lactate uptake into mouse cardiomyocytes
How doth the little busy bee: unexpected metabolism.
Hypoxia-induced carbonic anhydrase IX facilitates lactate flux in human breast cancer cells by non-catalytic function
NH4+ triggers the release of astrocytic lactate via mitochondrial pyruvate shunting
NH4+ triggers the release of astrocytic lactate via mitochondrial pyruvate shunting
Targeting of astrocytic glucose metabolism by beta-hydroxybutyrate
The Astrocyte: Powerhouse and Recycling Center
The astrocyte: powerhouse and recycling center
Transactivation activity and nucleocytoplasmic transport of beta-catenin are independently regulated by its C-terminal end
Fluorescent Nanosensor Based Flux Analysis: Overview and the Example of Glucose
Higher Transport and Metabolism of Glucose in Astrocytes Compared with Neurons: A Multiphoton Study of Hippocampal and Cerebellar Tissue Slices
Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate
Impaired brain energy metabolism in the BACHD mouse model of Huntington's disease: critical role of astrocyte-neuron interactions
Non-preferential fuelling of the Na+/K+ ATPase pump
Non-preferential fuelling of the Na+/K+-ATPase pump
Single-cell imaging tools for brain energy metabolism: a review
A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells
Metabolic signaling by lactate in the brain.
Small is fast: astrocytic glucose and lactate metabolism at cellular resolution
Acute feedback control of astrocytic glycolysis by lactate
Fast and Reversible Stimulation of Astrocytic Glycolysis by K+ and a Delayed and Persistent Effect of Glutamate
Fast and reversible stimulation of astrocytic glycolysis by K+ and a delayed and persistent effect of glutamate.
NBCe1 Mediates the Acute Stimulation of Astrocytic Glycolysis by Extracellular K+
NBCe1 mediates the acute stimulation of astrocytic glycolysis by extracellular K+
General requirement for harvesting antennae at ca and h channels and transporters.
Glucose and lactate supply to the synapse
High resolution measurement of the glycolytic rate.
Towards single-cell real-time imaging of energy metabolism in the brain
Kinetic validation of 6-NBDG as a probe for the glucose transporter GLUT1 in astrocytes
Preferential Transport and Metabolism of Glucose in Bergmann Glia over Purkinje Cells: A Multiphoton Study of Cerebellar Slices
A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells
Na+-Ca2+ cosignaling in the stimulation of the glucose transporter GLUT1 in cultured Astrocytes
An enquiry into metabolite domains
Antiribosomal-P autoantibodies from psychiatric lupus target a novel neuronal surface protein causing calcium influx and apoptosis
ATP steal between cation pumps: A mechanism linking Na + influx to the onset of necrotic Ca 2+ overload
ATP steal between cation pumps: a mechanism linking Na+ influx to the onset of necrotic Ca2+ overload
Why glucose transport in the brain matters for PET
A CYTOSOLIC SOURCE OF CALCIUM UNVEILED BY HYDROGEN PEROXIDE WITH RELEVANCE FOR EPITHELIAL CELL DEATH
Glutamate mediates acute glucose transport inhibition in hippocampal neurons
ALDOSE REDUCTASE INDUCED BY HYPEROSMOTIC STRESS MEDIATES CARDIOMYOCYTE APOPTOSIS - DIFFERENTIAL EFFECTS OF SORBITOL AND MANNITOL
Apoptotic and necrotic blebs in epithelial cells display similar neck diameters but different kinase dependency
Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy
Activation of GLUT1 by metabolic and osmotic stress: potential involvement of AMP-activated protein kinase (AMPK)
Ion movements in cell death: from protection to execution
Microcin E492, a channel-forming bacteriocin from Klebsiella pneumoniae, induces apoptosis in some human cell lines
Hyperosmotic shock induces both activation and translocation of glucose transporters in mammalian cells
Modulation of the two-pore domain acid-sensitive K+ channel TASK-2 (KCNK5) by changes in cell volume
Necrotic volume increase and the early physiology of necrosis
Nonselective cation channels as effecters of free radical-induced rat liver cell necrosis
Cytosolic [Ca2+] modulates basal GLUT1 activity and plays a permissive role in its activation by metabolic stress and insulin in rat epithelial cells
Sodium influx through non-selective cation channels: an effector mechanism for necrotic cell death
Measurement of sugar transport in single living cells
Evidence of two mechanisms for the activation of the glucose transporter GLUT1 by anisomycin: p38(MAP kinase) activation and protein synthesis inhibition in mammalian cells
Regulation of GLUT I in response to cellular stress
Clostridium botulinum C3 exoenzyme stimulates GLUT4-mediated glucose transport, but not glycogen synthesis, in 3T3-L1 adipocytes--a potential role of rho?
A chloride channel from human placenta reconstituted into giant liposomes.
Dissection of stress-activated glucose transport from insulin-induced glucose transport in mammalian cells using wortmannin and ML-9.
Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms.
Quantitation and immunolocalization of glucose transporters in the human placenta.
Trafficking of glucose transporters--signals and mechanisms.
Hypoxanthine transport in the guinea pig and human placenta is a carrier-mediated process that does not involve nucleoside transporters.
Adenosine transport and nitrobenzylthioinosine binding in human placental membrane vesicles from brush-border and basal sides of the trophoblast.
Effect of left intraventricular pressure on magnitude of vascular waterfall in the epicardial coronary veins.
Transport of amino acids and nucleosides in the placenta.
Closing pressure versus resistance in the regulation of coronary blood flow

Abstract (1)

Hypoxia-induced carbonic anhydrase IX facilitates lactate transport in human breast cancer cells by non-catalytic interaction

BookSection (1)

Activity-dependent regulation of glucose transport

BookWhole (1)

Activity-Dependent Regulation of Glucose Transporters

ConferencePoster (4)

Chaski a novel monocarboxylic acid transporter expressed in Central Nervous System of Drosophila.
Down-regulation of glucose transport in vascular smooth muscle cells by high extracellular glucose concentration.
Sweet or sour? ANLS QED.
Tight coupling of astrocyte energy metabolism to synaptic activity revealed in brain tissue with genetically encoded FRET nanosensors.

ConferenceProceedingWhole (10)

Fast fueling of information processing: roles of neurons and astrocytes
Multiscale integration of neuronal and astrocytic energy metabolism
Short-term control of astrocytic and neuronal metabolism as seen with optogenetic sensors
El despertar de la gliotransmisión metabólica
Novel molecular mechanisms of neurometabolic coupling revealed by FRET sensors for glucose, lactate and pyruvate
Novel optogenetic tools for the study of brain cell metabolism
Peeking into brain energy metabolism with genetically-encoded nanosensors nanosensors
Novel FRET nanosensors to probe neurometabolic coupling
Novel imaging tools for brain energy metabolism
Single - cell cancer metabolism: novel methods based on genetically - encoded FRET nanosensors

EditorialMaterial (2)

CROSSTALK Rebuttal from L. F. Barros and B. Weber
How doth the little busy bee: unexpected metabolism

Patent (3)

Genetically encoded probe for quantification of pyruvate concentration with high spatial and temporal resolution, and methods using the same for quantifying pyruvate transport, cellular pyruvate production and consumption, and mitochondrial pyruvate consumption in intact cells.
Method to measure the metabolic rate or rate of glucose consumption of cells or tissues with high spatiotemporal resolution using a glucose nanosensor
Genetically encoded probe for quantification of lactate with high spatial and temporal resolution, and methods for quantifying metabolic rates and lactate transport using the same.

Proyecto (11)

MECANISMOS MOLECULARES DE LA ACTIVACION DE GLUT1 POR HIPOXIA.
Development of a Platform for High-throughput Screening of Cell Metabolism
DINAMICA DE LA GLUCOSA EN ASTROCITOS: ESTUDIOS EN CULTIVO E IN SITU.
ESTUDIO DEL TRANSPORTE DE SUBSTRATOS METABOLICOS EN LA PLACENTA HUMANA Y ANIMAL
FAST MODULATION OF ASTROCYTIC MITOCHONDRIAL METABOLISM BY NEURONAL SIGNALS
MECANISMOS CELULARES DEL TRANSPORTE CEREBRAL DE GLUCOSA
MECANISMOS DE ACTIVACION DEL TRANSPORTE DE GLUCOSA POR ESTRES METABOLICO
PARTICIPACION DE TASK-2, UN CANAL DE K+ SENSIBLE A PH EXTRACELULAR, EN LA DISMINUCION DE VOLUMEN CELULAR HOMEOSTATICO Y APOPTOTICO.
PATOGENIA DE LA MUERTE NECROTICA EN CELULAS HEPATICAS: DISECCION DE MECANISMOS MOLECULARES EFECTOS.
SHORT TERM REGULATION OF ASTROCYTIC GLYCOLYSIS
SHORT-TERM MODULATION OF ASTROCYTIC LACTATE DYNAMICS BY NEURONAL SIGNALS

Review (3)

Metabolic signaling by lactate in the brain
Glucose and lactate supply to the synapse
A quantitative overview of glucose dynamics in the gliovascular unit
106
Luis Barros

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

13
Omar Porras

Assistant professor

Lab. Biología Celular

UNIVERSIDAD DE CHILE

Santiago, Chile

10
Alejandro San Martin

Senior Reseacher

Biología

CENTRO DE ESTUDIOS CIENTIFICOS

Valdivia, Chile

3
Viviana Montecinos

Assistant Professor

Hematology Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

3
Francisco Sepúlveda

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

3
Maria Niemeyer

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

2
Jimena Sierralta

Full Professor

Neuroscience

Faculty of Medicine, Universidad de Chile

Santiago, Chile

2
Mario Chiong

Profesor

Departamento de Bioquímica y Biología Molecular

Universidad de Chile

Santiago, Chile

2
Luis Cid

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

2
Steffen Härtel

Full Professor

Instituto de Ciencias Biomédicas

University of Chile

Santiago, Chile

2
Nibaldo Inestrosa

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Juan Vera

Full professor

Pathophysiology

University of Concepcion

Concepción, Chile

2
Andres Stutzin

Full Professor

ICBM

FACULTAD DE MEDICINA UNIVERSIDAD DE CHILE

Santiago, Chile

1
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Maria Matus

Principal Investigator

Fundacion Ciencia & Vida

Santiago, Chile

1
Patricio Smith

Associate Professor

Medicine / Dentistry

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Marcelo Catalan

Profesor Auxiliar

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile

1
Hernán Huerta

Asistente de Investigación

Centro de Biología Integrativa

Universidad Mayor de Chile

Santiago, Chile

1
Moisés Sandoval

Co-Investigador

Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

1
Pamela Sandoval

Postdoctoral Researcher

Biología

Centro de Estudios Científicos -CECs-

Valdivia, Chile

1
Ignacio Niechi

Profesor Adjunto

Facultad de Ciencias

Universidad Austral de Chile

Valdivia, Chile

1
Ricardo Delgado

Profesor Adjunto

Biología

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE BIOLOGIA

Santiago, Chile

1
CAROLINA AÑAZCO

Associate Professor

Preclinical Sciences

Universidad Católica del Maule

TALCA, Chile

1
Isabel Cornejo

Investigadora

Biología

Centro de Estudios Científicos

Valdivia, Chile

1
Nicolas Tobar

Assistan Professor

Cellular and molecular Biology Laboratory

INSTITUTO DE NUTRICION Y TECNOLOGIA DE LOS ALIMENTOS, INTA-UCHILE

Santiago, Chile

1
Rodrigo Quintanilla

Associate professor

Universidad Autónoma de Chile

Santiago, Chile

1
Carlos Oliva

Profesor Asistente

Biología Celular y Molecular

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Luis Michea

PROFESOR ASOCIADO

PROGRAMA FISIOLOGIA Y BIOFISICA

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Patricio Smith

Profesor Asociado

Odontología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
PATRICIA BURGOS

Associate Professor & Sub-Director CEBICEM

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

1
Maria Massardo

Docente Investigador

Medicina

Universidad San Sebastián

Santiago, Chile

1
Cristián Martínez

Researcher

CENTRO DE ESTUDIOS CIENTÍFICOS (CECs)

Valdivia, Chile

1
Luis González

Full Professor

School of Medicine

Universidad San Sebastián

Santiago, Chile

1
Rosalba Lagos

Professor

Biología

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS, DEPARTAMENTO DE BIOLOGÍA

Santiago, Chile

1
Eduardo Silva

Investigador Postdoctoral

Universidad Mayor

Santiago, Chile