Man

Juan Carlos Vera Carcamo

Full professor

University of Concepcion

Concepción, Chile

Líneas de Investigación


Biochemestry of Vitamin C Molecular and Structural biology of transports Antioxidants, vitamin C, oxidative stress

Educación

  •  Science, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1980
  •   Ciencias, mención en inmunología, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1981
  •  Science (Molecular Biology), University Autonoma of Madrid. España, 1993
  •  Science, Mention Molecular and Cell Biology, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1996

Experiencia Académica

  •   Full Professor Full Time

    UNIVERSIDAD DE CONCEPCION

    Biological Sciences

    Concepción, Chile

    2002 - A la fecha

  •   Associate Professor Full Time

    UNIVERSIDAD DE CONCEPCION

    Biological Sciences

    Concepcion, Chile

    2000 - 2003

Experiencia Profesional

  •   Full professor Full Time

    University of Concepcion

    Concepción, Chile

    1998 - A la fecha

  •   RESEARCH ASSOCIATE Full Time

    MEMORIAL SLOAN-KETTERING CANCER CENTER

    Estados Unidos

    1991 - 1992

  •   ASSISTANT MEMBER Full Time

    MEMORIAL SLOAN-KETTERING CANCER CENTER

    Chile

    1992 - 1995

  •   ASSOCIATE MEMBER Full Time

    MEMORIAL SLOAN-KETTERING CANCER CENTER

    Chile

    1995 - 1999

  •   associate professor Full Time

    University of Concepcion

    Chile

    2000 - 2003

  •   Director Departament Pathophysiology Part Time

    University of Concepcion

    Concepción, Chile

    2002 - A la fecha

  •   Director Departament Pathophysiology Other

    University of Concepcion

    Concepción, Chile

    2003 - A la fecha

  •   Director Phd program BM/ BC Biological SC Other

    University of Concepcion

    Concepción, Chile

    2003 - 2005

  •   Director Udec Biomedicine program Other

    University of Concepcion

    Concepcion, Chile

    2008 - A la fecha

Formación de Capital Humano


TEACHING Experience.
I. University of Concepción 2014.

Undergraduate
212600 Thesis Project Formulation (Biochemestry)
240405 Research Unit Biology,
240502 Proyect title seminar (Biología)
242554 Proyect title seminar, (Ing Marine Biotechnology and Aquaculture)
250307 Workshop V: Science Soc and Tec.ecnología y Sociedad (Bioengineering)
250401 Pathophysiology Topic Molecular Cancer Gene Therapy (Bioengineering)
250503 Advanced Cell Biology Topics, (Bioengineering)
250508 Thesis Bioengineering,
251121 Basic Cell Biology (Nursing)
251501 Bioq III Research Unit and Seminar Bibliographical, Special Biochemistry, (Biochemestry)
253205 Pathophysiology (Biochemistry)
253205 Physiology (Biochemistry)
253217 Physiology and Pathophysiology I
254202 Pathophysiology (Nursing)
254203 Pathophysiology (Obstetrics)
254210 Pathophysiology Fonoaudiología (Fonoaudiología)
254301 Pathophysiology Biochemistry (Biochemistry)
254302 Pathophysiology (Dentistry)
254303 Pathophysiology (Chemistry and Pharmacy)
254309 Cell culture Applications (Bioengineering, Biochemistry, Biol)
254311 Biosafety in Laboratory (Biochemistry)
251121 Basic Cell Biology (Nursing)
251501 Bioq III Research Unit and Seminar Bibliographical, Special Biochemistry (Biochemistry)
256206 Introduction to Biomedical Sciences II (Medicine)
256218 Introduction to Biomedical Sciences IV (Medicine)

733352 Práctica Traducción Inglés-Español III, Traducción / Interpretación en Idiomas Extranjeros

Postgrade Teaching

PhD Program in Biological Science, Cellular and Molecular Biology
4235991 Thesis proyect Formulation
4235991 Professor Thesis Committe Thesis proyect
406894 Research Unit I
406834 Research Unit II
406895 Bibliographic Seminar I
406831 Bibliographic Seminar II
406834 Unidad de Investigación II

Master in Biochemistry and Bioinformatics.
4115005 Research unit I
4115006 Research unit II
4115007 Bibliographic Seminar I
4115008 Bibliographic Seminar II
4115012 Thesis project formulation
4115013 Candidate

Master in Physiology .
401425 Topics on cancer pathophysiology
401427 Cell biology and pathology
4111003 Research Unit II
4111006 Topics on cancer pathophysiology
4111007 Seminar II
4111991 Thesis project formulation

Master program in Human Physiology

4111011 Introduction to Molecular Pathology
4111993 Thesis Dissertation
4111991 Seminar I

Obstetrics, Specialization in Gynecology
500603 Specialization in Gynecology for Obstetricians

Obstetrics, Specialization in en Perinatology
500554 Specialization in Perinatology for Obstetricians

Direction of grade Thesis

Direction of undergrade Thesis
1. Student: Felipe Ignacio Paredes Díaz. Career: Biochemestry, University of Concepción. Thesis: Molecular analysis of regulation of the expression of Vitamin C transporter in human cells CaCo2. Finalized 2010.

2. Student: David Andrés Escobar Flores. Career: Biochemestry, University of Concepcion. Thesis: Development of a method bioinformatic for the identification of amino acids with functional - structural importance of membrane proteins. Finalized 2010.

3. Student: Maria de Los Angeles Sepúlveda Parada. Career: Biochemestry, University of Concepcion. Thesis: Analysis of the functional importance of residues aminoacídicos of the domain of exofacial union of the carrier of acid ascórbico SVCT1. Finalized 2012.

4. Student: Karen Susan Sweet Alvarado. Career: Biochemestry, University of Concepcion. Thesis: Identification and functional characterization of cysteine residues with the potential regulatory in the ascorbic acid transporter SVCT2. Finalized 2012.

5. Student: Ibeth Alejandra González Galdames. Career: Biología, University of Concepcion. Thesis Seminar: Modulation of the transcriptional activity of rat promoter of SVCT1 mediated by exogenous oxidative stress in rat hepatoma cells H4IIE. Finalized 2012.

6. Student: Carolina Muñoz Flores. Career: Bioengineering, University of Concepcion. Thesis: Molecular tools directed the metabolism of vitamin C in cancerous cells by modifying SVCT2 transporter expression. Finalized Marzo 2013.

7. Student: Natalia Cristhy Belmar Sazón. Career: Engineering in Marine Biotechnology and Aquaculture, Faculty of Natural Sciences and Oceanography, University of Concepcion. Seminar Title: Development of molecular tools for the analysis of the systems of vitamin C transport in fishs. In Execution.

Professor Evaluation Committee of Undergraduate Thesis
1. Student: Francisco Javier Roa Cartes. Career: Biochemestry, University of Concepcion. Thesis Regulating the expression of Vitamin C transporter in response to oxidative stress in breast cancer cells. Finalized 2011.

2. Student: Jorge Luis Vera Becerra. Career: Biochemestry, Universidad Austral de Chile. Thesis: characterization of residues involved in exofacial junction of substrates in the ascorbic acid transporter ascórbico SVCT2. Finalized 2011.

4. Student: Marisel Dominique Medina Nuñez. Career: Biólogo, University of Concepcion. Seminario de Título: TrxR1 Participation in the accumulation and recycling of vitamin C on prostate cancer. Finalized 2011.

5. Student: Esteban Francisco Caamaño Molina. Career: Licenciatura en Biología, University of Concepcion. Reserch Unit: Standardization of an in vitro model of hepatic oxidative stress induced by carbon tetrachloride.. Finalized 2012.

6. Student: Paulina Andrea Saavedra Sieyes. Career: Licenciatura en Biología, University of Concepcion. Thesis: Expression of corepresores SMRT and NCOR in prostate cancer. Finalized 2012.

7. Student: Cristina Angélica Padilla Ruiz. Career: Biochemestry, University of Concepcion. Thesis: Cloning, expression and functional characterization of SVCT3, a new member of the family of ascorbic acid transporters. Finalized 2012.

8. Student: Esteban Francisco Caamaño Molina. Career: Biología, University of Concepcion. Thesis Seminar Biologist: Protective effect of glutathione in an in vitro model of hepatic oxidative stress induced by carbon tetrachloride. Finalized March 2013.

Direction of postgrade Thesis

Direction of Master Thesis
1. Student: Carolina Muñoz Flores. Career: Bioengineering, University of Concepcion. Thesis: Molecular alteration and pharmacology of metabolism of vitamin C in human cells. Thesis.

2. Student: Karen Susan Sweet Alvarado. Career: Biochemestry, University of Concepcion. Thesis: Saturating mutagenesis of the pathway translocation substrate in transporter ascorbic acid SVCT2.. Thesis.

Professor Committee Magister Thesis Evaluation
1. Student: Perla Kathanina Salgado Mejias. Programa: Master of Clinical Biochemistry and Immunology, University of Concepcion. Thesis Title: Protective effect of cellular GSH in the defense to oxidative stress by exposure to snuff smoke in human endofaciales cells. Finalized 2010.

2. Student: Claudia Solange Jaña Martínez. Programa: Master in Biochemistry and Bioinformatics, University of Concepcion. Título Thesis: Cloning, expression and characterization of dehydroascorbate reductase in human tumor cells. Finalized 2011.

3. Student: Pablo Antonio Binder Saldaña. Programa: Master in Biochemistry and Bioinformatics, University of Concepcion. Título Thesis: Functional characterization of the promoter region of reductase dehydroascorbic acid 1 glutarredoxina. Finalized 2011.

4. Student: Eduardo Andrés Peña Alarcón. Programa: Master in Science mention Physiology, University of Concepcion. Título Thesis: Differential expression of Vitamin C transporters in breast cancer cells negative for the estrogen receptor. Finalized 2011.

5. Student: Marilyn Denisse Vergara Valenzuela. Programa: Master of Clinical Biochemistry and Immunology, University of Concepcion. Thesis Title: Molecular analysis of the expression of Vitamin C transporters in colon cancer. Finalized 2012

Direction of Phd Thesis

1. Alexis Salas. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Development of robust methods for predicting 3D structures of membrane proteins and their application to the ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. Finalized 2011.

2. Student: María Elizabeth Escobar Sagredo. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mutational analysis of a domain-binding EF-hand calcium vitamin C transporter SVCT1. PhD Scholarship CONICYT. Finalized 2012.

6. Student: Henny Marianne Haensgen Sáez. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Structural determinants translocation pathway substrate in ascorbic acid transporter SVCT1. PhD Scholarship CONICYT. In Execution.

7. Student: Carlos Francisco Aylwin Ramírez. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Construction of a transporter without cysteine (Cys-less) and structural and functional characterization of the migration path substrates ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. In Execution.

8. Student: Paula Angélica Guzmán González. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Transcriptional regulation of vitamin C transporters SVCT1 and SVCT2 during differentiation and response to oxidative stress in human intestinal cells CaCo-2. PhD Scholarship CONICYT. In Execution.

9. Student: Alejandra Andrea Muñoz Vega. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Characterization of the promoter region of the gene for vitamin C transporter rat SVCT2. PhD Scholarship CONICYT. In Execution.

10. Student: Marcell Alejandra Gatica Miranda. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Quaternary structure of transporter vitamin C SVCT2. PhD Scholarship CONICYT. In Execution.

11. Student: Mafalda Eliana Maldonado Villagrán. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Early regulation and molecular determinants of vitamin C metabolism post-partial hepatectomy in rats. In Execution.

12. Student: Pamela Mendoza. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mutational analysis saturating pathway translocation substrate ascorbic acid transporter SVCT2. PhD Scholarship CONICYT. In Execution.

13. Student: Mauricio Alejandro Gonzalez Oyarzún. Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Mechanistic aspects and structural determinants of transport cycle ascorbic acid transporters plasma membrane and intracellular. PhD Scholarship MECESUP. In Execution.

14. Student: Francisco Javier Roa Cartes: Doctoral Program in Biological Science area Molecular and Cell Biology, University of Concepcion. Thesis: Role of transporter vitamin C SVCT2 in mitochondria in human cells. PhD Scholarship CONICYT. In Execution.


Difusión y Transferencia


EXTENSION

Transfer to community impact.

2010 Biomedicine Workshop 2.0. Designing a Science Company linking strategy, TABLE III: Workshop of Cluster BíoBío Health, Genetic Engineering Workshop for Leaders of Bio-Bio Region. The workshop was attended by representatives of companies, government agencies Chile earmarked to financing R & D and regional print media. Antioxidants Laboratory, School of Biological Sciences, University of Concepción, January 27, 2010.

2011 Symposium “New Pharmacological Therapies in Cancer. Molecular basis of antitumor therapy”. XXXIII Annual Congress of Chile Pharmacology Society, Olmué, Chile, 16-19 November 2011. Journal of Pharmacology, Chile 4(1): 29. Organizer.

Popular articles in magazines, newspapers and other media.

2010 Scientists for a Day: Genetic Engineering Workshop: Southern Journal, Sunday January 31, 2010.

2010 Interview Program Conceptions of TVU, "Research in Cancer" broadcast on Thursday 9th December at 20:0 pm and rebroadcast on Sunday 12th at 20:40 hours.

2012 UdeC Scientists create transgenic mice with novel technique.
Panorama UdeC, 26 de July.


Premios y Distinciones

  •   Premio Municipal de Ciencias

    ILUSTRE MUNICIPALIDAD DE CONCEPCION

    Chile, 2008

    Premio Municipal de Ciencias, Ilustre Municipalidad de Concepción, año 2008


 

Article (90)

Lectin-Like Oxidized LDL Receptor-1 Is an Enhancer of Tumor Angiogenesis in Human Prostate Cancer Cells
Concentration of ascorbic acid and antioxidant response in early life stages of Engraulis ringens and zooplancton during spawning sesions 2006-2009 off central Chile.
Concentration of ascorbic acid and antioxidant response in early life stages of Engraulis ringens and zooplankton during the spawning seasons of 2006-2009 off central Chile
Resolution of the direct interaction with and inhibition of the human GLUT1 hexose transporter by resveratrol from its effect on glucose accumulation
Concentration of ascorbic acid and innate immune effectors in Engraulis ringens and Strangomera bentincki during their main spawning period (2007-2008) in the Humboldt current system off Chile
Concentration of ascorbic acid and innate immune effectors in Engraulis ringens and Strangomera bentincki during their main spawning period (2007–2008) in the Humboldt current system off Chile
Concentration of ascorbic acid and innate inmune fectors in Engraulis ringens and Strangomera bentincki during their main spawning period (2007-2008) in the Humboldt current system off Chile
Essential role of intracellular glutathione in controlling ascorbic acid transporter expression and function in rat hepatocytes and hepatoma cells
Noncompetitive blocking of human GLUT1 hexose transporter by methylxanthines reveals an exofacial regulatory binding site
Hexose Transporter GLUT1 Harbors Several Distinct Regulatory Binding Sites for Flavones and Tyrphostins
Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells
Histidine Residues in the Na+-coupled Ascorbic Acid Transporter-2 (SVCT2) Are Central Regulators of SVCT2 Function, Modulating pH Sensitivity, Transporter Kinetics, Na+ Cooperativity, Conformational Stability, and Subcellular Localization
Endofacial competitive inhibition of the glucose transporter 1 activity by gossypol
Molecular Identification and Functional Characterization of the Vitamin C Transporters Expressed by Sertoli Cells
Mechanistic insights and functional determinants of the transport cycle of the ascorbic acid transporter SVCT2
OXIDIZED LOW DENSITY LIPOPROTEINS INDUCE APOPTOSIS IN HUMAN LYMPHOCYTES: INVOLVEMENT OF MITOGEN-ACTIVATED PROTEIN KINASES
Vitamin C is an essential antioxidant that enhances survival of oxidatively stressed human vascular endothelial cells in the presence of a vast molar excess of glutathione
Differential subcellular distribution of glucose transporters GLUT1-6 and GLUT9 in human cancer: Ultrastructural localization of GLUT1 and GLUT5 in breast tumor tissues
Redefining the facilitated transport of mannose in human cells: Absence of a glucose-insensitive, high-affinity facilitated mannose transport system
Sodium vitamin C cotransporter SVCT2 is expressed in hypothalamic glial cells
Vitamin C uptake and recycling among normal and tumor cells from the central nervous system
A cytosolic source of calcium unveiled by hydrogen peroxide with relevance for epithelial cell death
Differential regulation of glucose transporter expression by estrogen and progesterone in Ishikawa endometrial cancer cells
Differential signalling for enhanced hexose uptake by interleukin (IL)-3 and IL-5 in male germ cells
Predicting the three-dimensional structure of the human facilitative glucose transporter Glut1 by a novel evolutionary homology strategy: Insights on the molecular mechanism of substrate migration, and binding sites for glucose and inhibitory molecules
Estrogen and progesterone up-regulate glucose transporter expression in ZR-75-1 human breast cancer cells
Hypothalamic ependymal-glial cells express the glucose transporter GLUT2, a protein involved in glucose sensing
Recycling of vitamin C by a bystander effect
Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated CaCo-2 cells
Glycine receptors involved in synaptic transmission are selectively regulated by the cytoskeleton in mouse spinal neurons
Nicotinamide is not a substrate of the facilitative hexose transporter GLUT1
Nicotinarnide is not a substrate of the facilitative hexose transporter GLUT1
Direct inhibition of the hexose transporter GLUT1 by tyrosine kinase inhibitors
Elevated expression of glucose transporter-1 in hypothalamic ependymal cells not involved in the formation of the brain-cerebrospinal fluid barrier
Elevated expression of glucose transporter-1 in hypothalamic ependymal cells not involved in the formation of the brain–cerebrospinal fluid barrier
Expression of GM-CSF receptors in male germ cells and their role in signaling for increased glucose and vitamin C transport
High-affinity sodium-vitamin C co-transporters (SVCT) expression in embryonic mouse neurons
Mechanism of vitamin C inhibition of cell death induced by oxidative stress in glutathione-depleted HL-60 cells
The predicted ATP-binding domains in the hexose transporter GLUT1 critically affect transporter activity
The Predicted ATP-Binding Domains in the Hexose Transporter GLUT1 Critically Affect Transporter Activity †
High-affinity binding to the GM-CSF receptor requires intact N-glycosylation sites in the extracellular domain of the beta subunit.
Molecular characterization of a granulocyte macrophage-colony-stimulating factor receptor alpha subunit-associated protein, GRAP.
Defective glucose transport across brain tissue barriers: A Stromal cell oxidation: a mechanism by which tumors obtain vitamin C neurological syndrome
Defective glucose transport across brain tissue barriers: A newly recognized neurological syndrome
GLUT1-deficiency: barbiturates potentiate haploinsufficiency in vitro.
Kinetic resolution of two mechanisms for high-affinity granulocyte-macrophage colony-stimulating factor binding to its receptor.
STROMAL CELL OXIDATION: A MECHANISM BY WHICH TUMORS OBTAIN VITAMIN C
Deficient transport of dehydroascorbic acid in the glucose transporter protein syndrome
Expression of granulocyte-macrophage colony-stimulating factor receptors in human prostate cancer
Expression of the human GM-CSF receptor alpha subunit in Saccharomyces cerevisiae.
Hexose transporter expression and function in mammalian spermatozoa: Cellular localization and transport of hexoses and vitamin C
Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter.
Vitamin C crosses the blood-brain barrier as dehydroascorbic acid through the glucose transporters.
Chemical effects of multi-millicurie preparation of 6-[18F]-fluoro-6-deoxy-L-ascorbic acid via nucleophilic displacement with 18F-labelled fluoride
Efficient Transport and Accumulation of Vitamin C in HL-60 Cells Depleted of Glutathione
Human erythrocytes express GLUT5 and transport fructose
Increased facilitated transport of dehydroascorbic acid without changes in sodium-dependent ascorbate transport in human melanoma cells
Increased uptake and accumulation of vitamin C in human immunodeficiency virus 1-infected hematopoietic cell lines
Expression of the fructose transporter GLUT5 in human breast cancer
Genistein is a natural inhibitor of hexose and dehydroascorbic acid transport through the glucose transporter, GLUT1
Modeling facilitative sugar transporters: transitions between single and double ligand occupancy of multiconformational channel models explain anomalous kinetics.
Sodium-glucose cotransporters display sodium- and phlorizin-dependent water permeability.
Granulocyte-macrophage colony-stimulating factor signals for increased glucose uptake in human melanoma cells.
Membrane-associated and soluble granulocyte/macrophage-colony-stimulating factor receptor alpha subunits are independently regulated in HL-60 cells.
Modeling Facilitative Sugar Transporters: Transitions Between Single and Double Ligand Occupancy of Multiconformational Channel Models Explain Anomalous Kinetics
Multi-millicurie Preparation of 6-[^ 1^ 8F] Fluoro-6-deoxy-L-ascorbic Acid via Nucleophilic Displacement with^ 1^ 8F-Labelled Fluoride
Multifunctional transporter models: lessons from the transport of water, sugars, and ring compounds by GLUTs.
N-Glycosylation of the Human Granulocyte-Macrophage Colony-stimulating Factor Receptor   Subunit Is Essential for Ligand Binding and Signal Transduction
Identification of CRKL as the constitutively phosphorylated 39-kD tyrosine phosphoprotein in chronic myelogenous leukemia cells.
The acute chest syndrome in sickle cell disease: incidence and risk factors. The Cooperative Study of Sickle Cell Disease.
Evidence that facilitative glucose transporters may fold as beta-barrels.
Insulin-like Growth Factor-I Unresponsiveness in an Efe Pygmy
Role of facilitative glucose transporters in diffusional water permeability through J774 cells.
A possible role for a mammalian facilitative hexose transporter in the development of resistance to drugs.
Functional expression of murine multidrug resistance in Xenopus laevis oocytes.
Glucose transporters serve as water channels.
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
A discrete repeated sequence defines a tubulin binding domain on microtubule-associated protein tau
Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
The major component of the rat sperm fibrous sheath is a phosphoprotein
Heat-stable microtubule protein MAP-1 binds to microtubules and induces microtubule assembly.
Measurement of microgram quantities of protein by a generally applicable turbidimetric procedure
Small zone gel chromatography of interacting systems: Theoretical and experimental evaluation of elution profiles for kinetically controlled macromolecule-ligand reactions
Biosynthesis of rat sperm outer dense fibers during spermiogenesis. In vivo incorporation of [3H]leucine into the fibrillar complex.
Phosphoproteins are components of bull sperm outer dense fibers
Polypeptide composition of rat sperm outer dense fibers. A simple procedure to isolate the fibrillar complex.
A simple procedure to elute proteins from polyacrylamide gels
A simple and rapid method to prepare gradients
Arginyl residues involvement in the microtubule assembly
Isoacceptor glycine transfer-RNA species during bovine myocardium development

Abstract (8)

Detection and quantification of Helicobacter pylori cagA gene in gastric biopsies of Chilean patients by real-time PCR
Vitamin C metabolism in human cancer cells
Molecular identification and funcional characterization of the vitamin C transporters expressed by sertoli cells
Expression of vitamin C transporter SVCT2 in hypothalamic glial cells. An immunohistochemical and kinetic analysis
Hypothalamic ependymal cells express the glucose transporter GLUT2, a protein involved in glucose sensing
Differential subcellular distribution of the glucose transporter GLUT1 and the fructose transporter GLUT5 in breast tumour cells
Essential role of predicted nucleotide binding sites on the functional activity of glucose transporter GLUT1. Structural and regulatory implications
Glucose and vitamin C uptake in ependymal cells of brain hypothalamus. Glut1, Glut2 and SVCT expression and function.

ConferencePaper (11)

Differential Expression of Ascorbic Acid Transporters in Prostate Tumors
Functional importance of extracellular cystein residues in the ascorbic acid transporter SVCT2
Identification and characterization of residues involved in the ascorbic acid transport pathway in SVCT2
Transcriptional regulatión of the SVCT1 transporter in human intestinal CaCo-2 cells
Bone Stroma induces AR basal transcriptional activity in prostate cancer cells
Differential expression of vitamin C transporters in breast cancer
Effects on glutahtione in oxidative stress response in prostate cancer
Mutational analysis of SVCT1 between transmembrane domain XI and XII
Structural and functional determination of the Na+ binding domain in the ascorbic acid transporter SVCT1
Transcriptional regulation of glutaredoxin-1 in prostate cancer models undergoing oxidative stress
Regulation of ascorbic acid transporters SVCT1 and SVCT2 by the presence of Ca+2 and Mg+2 binding sites

ConferencePoster (47)

A Single Exofacial Histidine Residue Defines Ascorbic Acid Transporter SVCT2 Acid pH Sensitivity
Site-directed mutagenesis of exofacial amino acid residues involved in substrate binding in the ascorbic acid transporter
Site-directed mutagenesis of extracellular cysteine residues in the sodium-coupledascorbic acid transporter-2, SVCT2
A robust algorithm for the automated coupling of bioinformatic tools for membrane proteins 3D design and its application to vitamin C transporters
Oligomeric structure of the SVCT2 ascorbic acid transporter
The receptor for oxidized LDL, LOX-1, is expressed in human prostate cancer and induces an epithelial mesenchimal transition in DU-145 cells
Antioxidant metabolism in cancer cells
Changes in the estrogen receptor expression is associated with androgen-independent progression in prostate cancer
Cysteine residues modulate the functional properties of the ascorbic acid transporter SVCT2
Structure-function in the Na+ coupled ascorbic acid transporter-2
SpH-protease exists in Caco-2 cells
Characterization of the expression and function of the receptor LOX-1 in prostate cancer cells
Tumor cell-microenvironment interaction and the antioxidant potential of cancer cells
Vitamin C transporters in cancer: structural conservation and functional diversity
Ascorbic acid transport by SVCT1 is regulated by divalent cations
Cloning, expression and functional characterization of deshidroascorbic acid reductases from human tumor cells
Construction and functional analysis of the ascorbic acid transporter chimeras SVCT1-SVCT2
Flavonoids and methylxanthines regulated ascorbic acid uptake interacting an exofacial domain in SVCT2
Functional analysis of the facilitative hexose transporters GLUT10 and GLUT11, and characterization of novel dehydroascorbic acid transporters
Functional importance of extracellular cystein in ascorbic acid transporter SVCT2
Intracellular expression of ascorbic acid transporters in breast cáncer
Sodium vitamin C transporter SVCT2 three-dimensional model: Sustrate translocation pathway across the protein
The class III hexose transporter GLUT12 transports oxidized vitamin C
Altered androgen receptor-dependent transcriptional activity in tumoral prostatic cells in the presence of bone stromal cells
Analysis of dehydroascorbic acid reductases and their participation in the metabolism of intracellular vitamin C
Analysis of the presence of tryptase positive-mast cells (MCT) in human normal and neoplastic prostatic tissue
Augmented expression of vitamin C transporters in malignant tumoral tissue
Differential expression of ascorbic acid transporters in colon cancer
Expression of adenosine receptors in human endothelial progenitor cells
Expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in human tumor cells
Glutathione and vitamin C in the antioxidant defenses in endothelial cells exposed to cigarette smoke and oxidative stress
Identification of amino acid residues that form the endofacial ascorbic acid binding site in the ascorbic acid transporter SVCT2
Identification of amino acids of the sodium binding site in the transporter SVCT2
Regulation of dehydroascorbic acid reductases expression in prostate cancer cells exposed to oxidative stress
Role of cystein residues in modulating ascorbic acid transport by the SVCT1
Transcriptional regulation of the ascorbic acid transporter SVCT1 in human intestinal CaCo-2 cells
Vitamin C transport and accumulation in inmortalized human astrocytes
Characterization adenosine transport in human endotelial progenitor cells
Characterization of SVCT1 short a new splice variant SVCT1 with regulatory properties
Generation of lentiviral particles for silencing vitamin C transporter SVCT2 expression
Glutahione regulates the ascorbic acid transporters in hepatocytes and rat hepatoma cells
Identification of a highly regulatory region in the proximal promoter region of rat SVCT1
Identification of a species-specific regulatory sequence in the proximal promoter of rat SVCT1
Presence of an allosteric exofacial domain in glucose transporter GLUT1 potential pharmacological use
Subcellular acquisition of vitamin C in human cells
Subcellular expression of a vitamin C transporter in human cells
Using bioinformatics tools to identify amino acid involved in the interaction between flavonoids, methylxanthines and ATP with the transporter SVCT2

ConferenceProceedingWhole (2)

Exofacial nucleotide binding site-like regulate the function of ascorbic acid transporter SVCT2
Molecular basis of antitumor therapy

Patent (2)

CONTACTING THE CELL WITH AN AMOUNT OF DEHYDROASCORBIC ACID EFFECTIVE TO INCREASE THE CONCENTRATION OF ASCORBIC ACID IN THE CELL
Method for increasing the concentration of ascorbic acid in brain tissues of a subject

Proyecto (6)

Experimental validation of a family of molecules as activators of glucose transporters for use in the control of hyperglycemia characteristic of diabetes mellitus
Structural-molecular biology of ascorbic acid transporter function
Innovation in therapeutic strategies for the treatment of diabetes through a course of action complementary to insulin. Development activator molecules with specificity for regulatory sites glucose transporters
Structural-molecular biology of ascorbic acid transporters
Valuing our nanoworld: Action of small molecules in health and disease, a trip to the interior of the cell in 80 milliseconds
Identification, development and validation of new inhibitory molecules with selectivity by glucose transporters for their application in the control of hyperglycemia characteristics of diabetes mellitus, obesity and metabolic syndrome

Review (1)

Vitamin C transporters
174
Juan Vera

Full professor

Pathophysiology

University of Concepcion

Concepción, Chile

58
CORALIA RIVAS

Associated Professor

Pathophysiology

UNIVERSIDAD DE CONCEPCION

CONCEPCION, Chile

19
Alejandro Reyes

Profesor Titular

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

13
FRANCISCO NUALART

Full Professor and Scientific Director

Cellular Biology and Center for Advanced Microscopy CMA BIO-BIO

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

9
Viviana Montecinos

Assistant Professor

Hematology Oncology

Pontificia Universidad Catolica de Chile

Santiago, Chile

9
Alexis Salas

Assistant Professor

Pharmacology

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

9
Lorena Mardones

ASSOCIATED PROFESSOR

BASIC SCIENCES AND MORPHOLOGY

UNIVERSIDAD CATOLICA DE LA SANTISIMA CONCEPCION

CONCEPCION, Chile

9
Juan Carcamo

Profesor Titular

Instituto de Bioquímica y Microbiología

Universidad Austral de Chile

Valdivia, Chile

8
Teresa Caprile

Associate profesor

DPTO. BIOLOGIA CELULAR,

UNIVERSIDAD DE CONCEPCIÓN

Concepcion, Chile

7
Luis Aguayo

Full Professor

Department of Physiology

Universidad de Concepcion

Concepcion, Chile

7
Sergio Onate

Associate Professor

Medical Specialties

UNIVERSIDAD DE CONCEPCION, DEPARTAMENTO FISIOPATOLOGIA

concepcion, Chile

7
Allisson Astuya

profesor asociado

Departamento de Oceanografia de la Universidad de Concepción

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

6
María García

Profesor Titular

Biología Celular

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

6
Alejandro Yanez

Full professor

Faculty of Science

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

6
Alejandro Godoy

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

5
Katterine Salazar

Associate Professor

Cell Biology

University of Concepcion

Concepción, Chile

5
Felipe Zuñiga

Profesor Asistente

Bioquimica Clinica e Inmunologia

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

4
Paola Ojeda

Profesor Adjunto

Ciencias Pre-clinicas

Universidad Católica del Maule

Talca, Chile

4
Carola Millan

Profesor

de Ciencias

UNIVERSIDAD ADOLFO IBAÑEZ FACULTAD DE ARTES LIBERALES

Viña del Mar, Chile

4
Marcelo Bustamante

Director

Basisc Sciences

UNIVERSIDAD CATÓLICA DE LA SANTÍSIMA CONCEPCIÓN

CONCEPCIÓN, Chile

3
Humberto González

Professor

Institute of Marine and Limnological Sciences

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

3
Angara Zambrano

Assistant Professor

Biochemistry and Microbiology

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

3
Bernardo Krause

Associate Professor

Insituto de Ciencias de la Salud

Universidad de O'Higgins

Rancagua, Chile

3
Valeska Ormazabal

Profesor asistente

Farmacología

Universidad de Concepción

Concepción, Chile

3
Ilona Concha

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

2
Mauricio Vargas

Head of R&D

R&D

Kura Biotech

Puerto Varas, Chile

2
María Rauch

ACADEMICO

INSTITUTO DE BIOQUÍMICA Y MICROBIOLOGIA

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

2
Mauricio Pinto

Investigador

STORM SPA

Santiago, Chile

2
Brigitte van Zundert

Full Professor

UNIVERSIDAD ANDRÉS BELLO, CENTRO DE INVESTIGACIONES BIOMÉDICAS

Santiago, Chile

2
Andres Carvajal

Investigador

INIA

Osorno, Chile

2
Ana Meneses

Académico

Universidad Diego Portales

Santiago, Chile

2
Liliana Lamperti

Vicedecana

UNIVERSIDAD DE CONCEPCIÓN, FACULTAD DE FARMACIA

Concepción, Chile

2
Maria Escobar

Postdoctorado

Biología Molecular Vegetal

Universidad de Chile

Santiago , Chile

2
Paula Sotomayor

Investigador

Medicina

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Gareth Owen

Profesor Titular

Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Viviana Ulloa

Profesional Superior

Dirección de Estudios Estratégicos

Universidad de Concepción

Concepción, Chile

2
MARCELO VILLAGRAN

Profesor asociado

Departamento de Ciencias Básicas

Universidad Catolica de la Santísima Concepción

Concepcion, Chile

2
Fernando Delgado

Associate Professor

Division of Medicine

Universidad Catolica del Maule

Talca, Chile

2
Leonardo Castro

Profesor Titular

Oceanografía

UNIVERSIDAD DE CONCEPCION

Concepción, Chile

2
Fernando Martinez

Assistant Professor

Cell Biology

Universidad de Concepción

Concepción, Chile

1
Alejandra Llanos

Profesor Asociado

Oceanografia

Universidad de Concepción

Concepción, Chile

1
Juan Tapia

Profesor

UNIVERSIDAD DE TALCA

Talca, Chile

1
Gustavo Moraga

Profesor Asistente

Departamento de Fisiología

Universidad De Concepción

Concepción, Chile

1
Jaime Madariaga

ASSOCIATE PROFESSOR

MEDICAL SPECIALTIES

UNIVERSITY OF CONCEPCION

CONCEPCION, Chile

1
Enrique Guzman

Profesor Asistente

Bioquímica Clínica e Inmunología

Universidad de Concepción

Concepción, Chile

1
Estefania Nova

Assistant Professor

Clinical Biochemistry & Immunology

University of Concepcion

Concepción , Chile

1
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Pablo Strobel

Prrofesional de laboratorio

Facultad Ciencias Veterinarias

Universidad Austral de Chile

Valdivia, Chile

1
mauricio gonzalez

phd

fisiopatologia

universidad de concepción

concepcion, Chile

1
LUIS SOBREVIA

Professor

Division of Obstetrics and Gynaecology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

1
Carlos Figueroa

Head

Anatomy, Histology and Pathology

Universidad Austral de Chile

Valdivia, Chile

1
Maria Poblete

Profesor Auxiliar

Anatomia, Histologia y Patologia

Universidad Austral de Chile

Valdivia, Chile

1
Maite Castro

Professor

Faculty of Sciences

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Mirna Muñoz

Profesor Asistente

Ciencias Básicas

UNIVERSIDAD CATÓLICA DE LA SANTÍSIMA CONCEPCIÓN

Concepción, Chile

1
Fredy Diaz

ASISTENTE

CIENCIAS BÁSICAS

UNIVERSIDAD CATÓLICA DE LA SANTÍSIMA CONCEPCIÓN

Concepción, Chile

1
Rodrigo Maldonado

Profesor Asociado

Vicerrectoria de Investigación

Universidad Mayor de Chile

Temuco, Chile

1
Oliberto Sanchez

Associate Professor

Pharmacology

University of Concepción

Concepción, Chile

1
Apolinaria García

Investigador

Microbiology

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

1
Carlos González

Profesor Asociado

Microbiology

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

1
Jorge Toledo

Director de Departamento

Fisiopatología

Universidad de Concepción

Concepcion, Chile

1
PAULA GUZMÁN

Profesional Bioquímico

Laboratorio Clínico

Hospital Clínico Regional de Concepción Guillermo Grant Benavente

Concepción, Chile

1
Enrique Werner

Académico

Ciencias Básicas

UNIVERSIDAD DEL BIO-BIO

Chillán, Chile

1
Luis Barros

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

1
Carola Muñoz

Profesor Asistente

Fisiología

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

1
ALEXIS HUMERES

DIRECTOR

MORFOFUNCIÓN

Universidad Diego Portales

SANTIAGO, Chile

1
Carlos Salomón

Academic

University of Concepcion

Concepcion, Chile

1
Natalie Parra

Colaborador Académico

Farmacología

Universidad de Concepción

Concepción, Chile

1
Claudio Aguayo

Profesor Asociado

Bioquimica Clinica e Inmunologia

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile