Man

Diego E Varela Lekanda

Profesor Asociado

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

Líneas de Investigación


Voltage dependent Calcium channel in hypertrophy; Chloride channels: structure, function and activation mechanisms

Educación

  •  Ciencias Básicas con Mención en Física, Universidad de Chile. Chile, 2004
  •  Doctor en Ciencias Biomédicas, Universidad de Chile. Chile, 2004

Experiencia Académica

  •   Profesor Asistente Full Time

    Universidad de Chile

    Santiago, Chile

    2008 - 2019

  •   Post-Doctorado Jornada Completa

    Universidad de Chile

    Santiago, Chile

    2004 - 2004

  •   Post-Doctorado Jornada Completa

    University of Calgary

    Calgary, Canada

    2005 - 2008

  •   Profesor Asociado Full Time

    UNIVERSIDAD DE CHILE

    Facultad de Medicina

    Santiago, Chile

    2019 - A la fecha

Experiencia Profesional

  •   Techician Full Time

    CECS

    Valdivia, Chile

    2000 - 2002

Formación de Capital Humano


Thesis Direction since 2008
Undergraduate Thesis
-Sergio Linsambarth (2014). Biotechnology. Faculty of Science, Universidad de Antofagasta, Antofagasta.
-Edgardo Salamanca (2013). Biotechnology, Faculty of Science, Universidad de la Frontera, Temuco.
-Tamara Veliz (Professional Practice, 2012). Biology, Faculty of Science, Universidad Católica de Chile.
-Edgardo Salamanca (Professional Practice, 2012). Biotechnology, Faculty of Science, Universidad de la Frontera, Temuco.

Graduate Thesis
-Cristian Moreno (2012), Master in Cell Biology. Facultad de Medicina, Universidad de Chile.

Undergraduate thesis in progress
-Hilda Alfaro. Biotechnology. Faculty of Science, Universidad Arturo Prat, Iquique. (Expected graduation date: August 2014).
- Nayareth Hidalgo. Biotechnology. Faculty of Science, Universidad de Antofagasta, Antofagasta. (Expected graduation date: August 2014).
Graduate thesis in progress.
-Cristian Moreno, PhD in Neuroscience. Facultad de Ciencias Biológicas, Universidad de Santiago de Chile.
-Matías Encina, Master in Biotechnology. Universidad Andrés Bello.
-Danna Morales, Master in Biotechnology. Universidad Andrés Bello.



 

Article (39)

KCTD5, a novel TRPM4-regulatory protein required for cell migration as a new predictor for breast cancer prognosis
TRPM7 Mediates Kidney Injury, Endothelial Hyperpermeability and Mortality During Endotoxemia
Calcium-dependent inactivation controls cardiac L-type Ca2+ currents under beta-adrenergic stimulation
EB1-and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion
Expression Suppression and Activity Inhibition of TRPM7 Regulate Cytokine Production and Multiple Organ Dysfunction Syndrome During Endotoxemia: A New Target for Sepsis
OxHDL controls LOX-1 expression and plasma membrane localization through a mechanism dependent on NOX/ROS/NF-κB pathway on endothelial cells.
FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway
Insights into the HyPer biosensor as molecular tool for monitoring cellular antioxidant capacity
Preventive Leptin Administration Protects Against Sepsis Through Improving Hypotension, Tachycardia, Oxidative Stress Burst, Multiple Organ Dysfunction, and Increasing Survival
Preventive Leptin Administration Protects Against Sepsis Through Improving Hypotension, Tachycardia, Oxidative Stress Burst, Multiple Organ Dysfunction, and Increasing Survival.
Prolonged AT(1)R activation induces Ca(V)1.2 channel internalization in rat cardiomyocytes
Ca-v beta(2) transcription start site variants modulate calcium handling in newborn rat cardiomyocytes
Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the Ca2+-activated non-selective cation channel TRPM4
Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel
Suppression of transient receptor potential melastatin 4 expression promotes conversion of endothelial cells into fibroblasts via transforming growth factor/activin receptor-like kinase 5 pathway
TRPM4 Is a Novel Component of the Adhesome Required for Focal Adhesion Disassembly, Migration and Contractility
Endotoxin Induces Fibrosis in Vascular Endothelial Cells through a Mechanism Dependent on Transient Receptor Protein Melastatin 7 Activity
Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel.
Targeting the Transient Receptor Potential Vanilloid Type 1 (TRPV1) Assembly Domain Attenuates Inflammation-induced Hypersensitivity
Increased Expression of the Transient Receptor Potential Melastatin 7 Channel Is Critically Involved in Lipopolysaccharide-Induced Reactive Oxygen Species-Mediated Neuronal Death
L-type calcium channel beta subunit modulates angiotensin II responses in cardiomyocytes
Transient receptor potential melastatin 4 inhibition prevents lipopolysaccharide-induced endothelial cell death
Hydrogen Peroxide Removes TRPM4 Current Desensitization Conferring Increased Vulnerability to Necrotic Cell Death
P2X4 Activation Modulates Volume-sensitive Outwardly Rectifying Chloride Channels in Rat Hepatoma Cells
Scanning mutagenesis of the I-II loop of the Cav2.2 calcium channel identifies residues Arginine 376 and Valine 416 as molecular determinants of voltage dependent G protein inhibition.
D1 Receptors Physically Interact with N-Type Calcium Channels to Regulate Channel Distribution and Dendritic Calcium Entry
Differential interactions of Na+ channel toxins with T-type Ca2+ channels.
Making the T-type even tinier: corticotropin-releasing factor-mediated inhibition of low-voltage-activated calcium channel activity
Use 'em and lose 'em-activity-induced removal of calcium channels from the plasma membrane
Activation of H2O2-induced VSOR CI(-)currents in HTC cells require phospholipase C gamma 1 phosphorylation and Ca2+ mobilisation
Extended spectrum of idiopathic generalized epilepsies associated with CACNA1H functional variants
Regulation of T-type calcium channels by Rho-associated kinase.
Hydroxyl radical activation of a Ca2+-sensitive nonselective cation channel involved in epithelial cell necrosis
NAD(P)H oxidase-derived H2O2 signals chloride channel activation in cell volume regulation and cell proliferation
The voltage-dependent CIC-2 chloride channel has a dual gating mechanism
The voltage-dependent ClC-2 chloride channel has a dual gating mechanism
The volume-activated chloride current depends on phospholipase c activation and intracellular calcium mobilization
Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel
Non-selective cation channels and oxidative stress- induced cell swelling

Abstract (4)

Vascular endothelial fibrosis induced by endotoxin: characteristics, mechanism and therapeutic perspectives
SENSING FUNCTION OF CL- CHANNELS. DOES IT MAKE SENSE?
Protons and calcium modulate the open probability of a non selective cation channel in epithelial cells
Effect of mutation C256A in rat ClC-2 on its gating behavior

Proyecto (15)

Millennium Nucleus of ion channels-associated diseases (MiNICAD)
Millennium Nucleus of Ion Channels-Associated Diseases, Minicad
CAVBETA2 SUBUNITS AND BETA-ADRENERGIC STIMULATION MODULATES THE C-TERMINAL AUTOINHIBITORY COMPLEX OF CAV1.2
CaVβ2 subunits and β-adrenergic stimulation modulate the C-terminal autoinhibitory complex of CaV1.2
Regulation of the trafficking and activity of TRPM4 by End Binding (EB) proteins and its role during cell migration
REGULATION OF THE TRAFFICKING AND ACTIVITY OF TRPM4 CHANNELS BY END BINDING (EB) PROTEINS AND ITS ROLE DURING CELL MIGRATION
Role of Beta-arrestin and CaVb2 splice variants in the internalization of the macromolecular complex CaV1.2/AT1
Role of β-arrestin and CaVβ2 splice variants in the internalization of the macromolecular complex CaV1.2 /AT1
SEPSIS-INDUCED ENDOTHELIAL FIBROSIS=> ROLE OF TRPM4 AND TRPM7 ION CHANNELS AS NOVEL TARGETS FOR PREVENTION OF ENDOTHELIAL FIBROSIS DURING SEPSIS
CEMC-FONDAP
The role of Calcium Channel Beta Subunits in the Modulation of L-type Calcium Currents by Angiotensin II in Cardiomyocytes
The Role of Calcium Channel Beta Subunits in the Modulation of L-type Calcium Currents by Angiotensin in Cardiomyocytes
Molecular determinants of T-type calcium channel inactivation and its physiological significance
Physiological effects of T-type calcium channel mutations linked to childhood absence epilepsy
Sepsis-induced endothelial fibrosis: Role of TRPM4 and TRPM7 ion channels as novel targets for prevention of endothelial fibrosis during sepsis

Review (1)

Oxidative stress-modulated TRPM ion channels in cell dysfunction and pathological conditions in humans
47
Diego Varela

Profesor Asociado

Fisiología y Biofísica

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

20
Felipe Simon

Profesor Titular

Department of Biological Sciences

UNIVERSIDAD ANDRES BELLO FAC DE CS BIOLOGICAS, DPTO DE CS BIOLOGICAS

Santiago, Chile

11
Andres Stutzin

Full Professor

ICBM

FACULTAD DE MEDICINA UNIVERSIDAD DE CHILE

Santiago, Chile

9
Ricardo Armisen

Associate Professor

Centro de Genética y Genómica

Universidad del Desarrollo

Santiago, Chile

7
Darwin Leiva

Assistant professor

Biology

Universidad de Santiago de Chile

Santiago, Chile

7
Oscar Cerda

Associate Professor

Molecular and Cellular Biology

Universidad de Chile

Santiago, Chile

6
Claudio Cabello

Full Professor

Ciencias Biologicas

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

6
César Echeverría

DIRECTOR DE INVESTIGACIÓN

Medicina

Universidad de Atacama

COPIAPO, Chile

5
Monica Caceres

Profesor asistente

Instituto de ciencias biomedicas

Universidad de Chile

Santiago, Chile

4
Omar Porras

Assistant professor

Lab. Biología Celular

UNIVERSIDAD DE CHILE

Santiago, Chile

4
Ana Riveros

Profesor Asistente

Departamento de Química Farmacológica y Toxicológica

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS QUIMICAS Y FARMACÉUTICAS

Santiago, Chile

3
Leandro Zuñiga

Profesor Asociado

Medicine

Universidad de Talca

Talca, Chile

3
Luis Cid

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECs

Valdivia, Chile

2
Maria Niemeyer

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTIFICOS CECS

Valdivia, Chile

2
Francisco Sepúlveda

Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS (CECS)

Valdivia, Chile

2
Pablo Olivero

Profesor Adjunto

Patologia y Fisiologia

Universidad de Valparaiso

Viña del Mar, Chile

2
Alvaro Becerra

Coordinador Academico

Ciencias Químico y Biológicas

Universidad Bernardo O'Higgins

Santiago, Chile

2
Luis Velasquez

Director and Full Professor

Medicine

Universidad SEK

Santiago, Chile

2
Ariela Vergara

Assistant Professor

Bioinformatics

UNIVERSIDAD DE TALCA

Talca, Chile

2
Alicia Colombo

Profesor Asistente/Director Científico Biobanco de Tejidos y Fluidos de la Universidad de Chile

Departamento de Anatomía Patológica

Universidad de Chile

Santiago, Chile

1
Antonello Penna

Profesor Asociado

Anestesiología y Medicina Perioperatoria

Universidad de Chile

Santiago, Chile

1
Pablo Tapia

Jefe Técnico Unidad de Paciente Crítico (UPC)

Unidad Paciente Critico (UPC)

Hospital Clinico Metropolitano La Florida

Santiago, Chile

1
Claudia Riedel

Full Professor

Life Sciences

Universidad Andres Bello

Santiago, Chile

1
Alvaro Elorza

ASSOCIATE PROFESSOR

INSTITUTE OF BIOMEDICAL SCIENCES

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Hector Contreras

Academico

Oncologia Basico Clinica

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, INSTITUTO DE CIENCIAS BIOMÉDICAS, PROGRAMA DE FISIOLOGÍA Y BIOFÍSICA

Santiago, Chile

1
Marcelo Catalan

Profesor Auxiliar

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile

1
Nicolas Tobar

Assistan Professor

Cellular and molecular Biology Laboratory

INSTITUTO DE NUTRICION Y TECNOLOGIA DE LOS ALIMENTOS, INTA-UCHILE

Santiago, Chile

1
Pablo Díaz

Professional/Scientific advisor

Scientific Affairs

Instituto de Salud Públida de Chile

santiago, Chile

1
Carolina Conejeros

Enfermera

Nefrología

Hospital Clínico de la Universidad de Chile

Santiago, Chile

1
Miguel Concha

Full Professor

Anatomy and Developmental Biology

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Claudio Perez

Profesor Asistente

Departamento de Ciencias Fisiológicas

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
Antonello Penna

Profesor Asociado

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Wendy Gonzalez

Director of the Center for Bioinformatics, Simulations and Modeling (CBSM)

Bioinformática

CENTRO DE BIOINFORMÁTICA Y SIMULACIÓN MOLECULAR (CBSM)/ UNIVERSIDAD DE TALCA

Talca, Chile

1
Camilo Campusano

Doctorante

Genética Molecular y Microbiología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
IVAN GALLEGOS

PATHOLOGIST, LABORATORY DIRECTOR

PATHOLOGY

HOSPITAL CLINICO DE UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Juan Santibáñez

Professor of Research

Molecular Oncology

Institute for Medical Research

Belgrade, Serbia

1
Fabián Jaña

Profesor Asistente

Departamento de Ciencias de la Salud

Universidad de Aysén

Coyhaique, Chile