Man

GONZALO ANTONIO MARDONES COFRE

Associate Professor

Universidad Austral de Chile

Valdivia, Chile

Líneas de Investigación


Biología celular y molecular; Tráfico de proteí­nas en las rutas secretoria y endocítica; Breast cancer; Protein trafficking; Structural aspects of membrane trafficking; Golgi structure and function; Protein crystallography; Structural biology

Educación

  •  Cell & Molecular Biology, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2003
  •  Biochemistry, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1995
  •  Bioquímico, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1997

Experiencia Académica

  •   Assistant Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2010 - 2017

  •   Associate Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2019 - A la fecha

  •   Associate Professor Part Time

    Universidad San Sebastián

    Medicine & Science

    Santiago, Chile

    2017 - 2018

  •   Assistant Professor Part Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2017 - 2018

  •   Instructor Part Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    1998 - 1999

Experiencia Profesional

  •   Research Fellow Full Time

    National Institutes of Health

    Bethesda, Estados Unidos

    2008 - 2010

  •   Postdoctoral Fellow Full Time

    National Institutes of Health

    Bethesda, Estados Unidos

    2005 - 2008

  •   Postdoctoral Fellow Full Time

    University of Colorado Health Sciences Center

    Denver, Estados Unidos

    2003 - 2005

Formación de Capital Humano


2020 - Catalina Meléndez, Ph.D. Thesis student, Cell Biology & Biomedicine, Universidad San Sebastián. Efecto de GOLPH3 y su localización en el aparato de Golgi sobre la función mitocondrial y la agresividad tumoral en células de cáncer de mama. (Co-patrocinante).
2019 - 2021 Rodrigo Castro, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. "Validación y caracterización de la interacción entre la oncoproteína de Golgi GOLPH3 y la GTPasa RAB2A". (Patrocinante).
2017 - 2020 Viviana Cavieres, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. Rol de GOLPH3 en la función de lisosomas de células tumorales. (Patrocinante).
2016 - 2019 Charlotte Luchsinger, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. GOLPH3 promotes the tumor phenotype favoring chemoresistance and cell migration in T98G cells of glioblastoma multiforme. (Patrocinante).
2014 - 2017 Cecilia Arriagada, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. Participación de GOLPH3 en la vía EGFR/mTOR y su rol en el fenotipo tumoral de células T98G de glioblastoma multiforme. (Patrocinante).
2013 - 2015 Gustavo Leal, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Función de la degradación de proteínas asociado a retículo endoplásmico, en control de los niveles de la proteína precursora del amiloide. (Co-patrocinante).
2013 - 2015 María José Tenorio, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Análisis del comportamiento de GOLPH3 en células tumorales de glándula mamaria humana mediante fluorescence recovery after photobleaching (FRAP). (Patrocinante).
2012 - 2014 Yennyfer Arancibia, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Participación de la proteína de tegumento VP11/12 del HSV-1 en la alteración del aparato de Golgi y eventos neurodegenerativos tempranos. (Co-patrocinante).
2012 - 2013 Silvia Ojeda, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización de la interacción proteína-proteína de alfa-SNAP wild type y de su mutante M105I. (Co-patrocinante).
2012 - 2014 Rodrigo Oyarzún, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Estudios funcionales de mutantes del transportador facilitativo de glucosa Glut-1. (Co-patrocinante).
2011 - 2014 Diego Acuña, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Análisis preliminar del proteoma del aparato de Golgi de células tumorales MCF-7 de glándula mamaria humana. (Tutor).
2011 - 2012 Camilo Muñoz, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Estudio de las consecuencias de la mutación puntual M105I en la proteína alfa-SNAP en su capacidad de interacción con membrana plasmática. (Co-patrocinante).
2010 - 2013 Esteban Corales, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización de sitios de unión para señales de destinación en la subunidad ?2 del complejo adaptador de proteínas AP-2. (Tutor).
2010 - 2013 Breyan Ross, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización estructural y funcional de los sitios de unión a señales de tirosina del complejo adaptador AP-1A. (Tutor).


Premios y Distinciones

  •   Medalla Conmemorativa 2 de Octubre, Categoría Ciencias

    Gobierno Región de Los Ríos

    Chile, 2016

    Distinción anual otorgada por contribución a la Región de Los Ríos, Chile, en categoría Ciencias

  •   Honorable Mention Celldance 2007

    American Society for Cell Biology

    Estados Unidos, 2007

    Distinction for contribution to outreach in Cell Biology in form of videomicroscopy

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2007

    Postdoctoral fellowship

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2006

    Postdoctoral Fellowship

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2008

    Postdoctoral Fellowship

  •   Travel Award

    American Society for Cell Biology

    Estados Unidos, 2001

    Predoctoral travel award to participate in the 41st Annual Meeting of the American Society for Cell Biology, December 8-12, Washington DC, USA

  •   Travel Award

    American Society for Biochemistry and Molecular Biology

    Estados Unidos, 2000

    Predoctoral travel award to participate in the Annual meeting of the American Society for Biochemistry and Molecular Biology, June 4-8, Boston, MA, USA

  •   Beca de doctorado

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 1997

    Beca para estudiantes de doctorado


 

Article (35)

GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation
Human Golgi phosphoprotein 3 is an effector of RAB1A and RAB1B
Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded Forster Resonance Energy Transfer Indicator
Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded FRET Indicator
The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-to-ER Retrograde Transport
Galectin-8 induces endothelial hyperpermeability through the eNOS pathway involving S-nitrosylation-mediated adherens junction disassembly
KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover
The knocking down of the oncoprotein Golgi phosphoprotein 3 in T98G cells of glioblastoma multiforme disrupts cell migration by affecting focal adhesion dynamics in a focal adhesion kinase-dependent manner.
Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling
HIV/SIV-Nef=> Pas de trois Choreographies to Evade Immunity.
Autophagosomes cooperate in the degradation of intracellular C-terminal fragments of the amyloid precursor protein the MVB/lysosomal pathway.
Autophagosomes cooperate in the degradation of intracellular C-terminal fragments of the Amyloid Precursor Protein via the MVB/lysosomal pathway
CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the gamma 1 and gamma 2 subunits of the AP-1 complex in protein trafficking
α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility.
Distinct biochemical pools of Golgi phosphoprotein 3 in the human breast cancer cell lines MCF7 and MDA-MB-231
Inhibition of the Hantavirus Fusion Process by Predicted Domain III and Stem Peptides from Glycoprotein Gc.
Inhibition of the Hantavirus Fusion Process by Predicted Domain III and Stem Peptides from Glycoprotein Gc.
Protein Kinase A Activity is Necessary for Fission and Fusion of Golgi to Endoplasmic Reticulum Retrograde Tubules
Protein kinase A activity is necessary for fission and fusion of Golgi to endoplasmic reticulum retrograde tubules.
Tetrahydrohyperforin Inhibits the Proteolytic Processing of Amyloid Precursor Protein and Enhances Its Degradation by Atg5-Dependent Autophagy
Co-assembly of viral envelope glycoproteins regulates their polarized sorting in neurons
Co-assembly of viral envelope glycoproteins regulates their polarized sorting in neurons.
Structural and functional characterization of cargo-binding sites on the mu4-subunit of adaptor protein complex 4
Structural and functional characterization of cargo-binding sites on the μ4-subunit of adaptor protein complex 4.
Structural basis for the recognition of tyrosine-based sorting signals by the mu3A subunit of the AP-3 adaptor complex
Turnover of C99 is controlled by a crosstalk between ERAD and ubiquitin-independent lysosomal degradation in human neuroglioma cells
AGAP2 regulates retrograde transport between early endosomes and the TGN
Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex
Sorting of the Alzheimer's disease amyloid precursor protein mediated by the AP-4 complex
Termination of autophagy and reformation of lysosomes regulated by mTOR
Analysis of Articulation Between Clathrin and Retromer in Retrograde Sorting on Early Endosomes
The trans-Golgi network accessory protein p56 promotes long-range movement of GGA/clathrin-containing transport carriers and lysosomal enzyme sorting
Selective plasma membrane permeabilization by freeze-thawing and immunofluorescence epitope access to determine the topology of intracellular membrane proteins
Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent
Hypothalamic-pituitary-adrenal axis function in patients with active rheumatoid arthritis: A controlled study using insulin hypoglycemia stress test and prolactin stimulation

Abstract (2)

APIB adaptor complex mediates basolateral sorting in a post-TGN compartment at the crossroads of the biosynthetic and recycling pathways
An autoantigen contained in a cytosolic protein complex associates with the trans-Golgi network (TGN)

Proyecto (19)

Evolution of the N-terminal region of the Golgi phosphoprotein 3 (GOLPH3) oncoprotein
Partitioning of the oncoprotein GOLPH3 in biomolecular condensates by liquid-liquid phase separation: a mechanism for the phenotypic plasticity of breast cancer cells
The interplay between phosphorylation and ubiquitylation controls stabilization of HERPUD1 under ER stress: possible implications on TNBC aggressiveness
Selective catabolic degradation of the endoplasmic reticulum by an Atg5-independent mechanism=> identification of molecular players
Characterization of new protein interactions of the Golgi protein GOLPH3 in cancer cell lines=> identification of new therapeutic targets
Unidad de cristalización de proteínas
Microscopio Spinning-Disk
Núcleo de Investigación de Excelencia CISNe=> Centro Interdisciplinario de Estudios del Sistema Nervioso
Vigilancia activa de hantavirus
Desarrollo de una plataforma de análisis de interacciones moleculares para el fortalecimiento de la investigación en ciencias biomédicas y biotecnológicas en la Universidad Austral de Chile
Tracking newly-synthesized APP through the secretory pathway with the RUSH system under normal and cellular stress conditions in neuronal cells
Use of siRNA screening to identify ubiquitin-related pathways involved in Alzheimer's Disease
Golgi proteome in cancer=> identification of new therapeutic targets
Protective degradation of amyloid precursor protein regulated by ER stress, ERAD, and sorting into lysosomes through MVBs and autophagosomes
Modulation of the stress sensors AMPK- and Sirtuin 1-dependent pathways during Herpes simplex virus type 1 neuronal infection
MOLECULAR CHARACTERIZATION OF A NEW CARGO-BINDING SITE ON MU-SUBUNITS OF ADAPTOR PROTEIN COMPLEXES; IMPLICATIONS FOR PROTEIN TRAFFICKING AND CELL PHYSIOLOGY.
ROLE OF THE MULTIVESICULAR BODY/LYSOSOMAL PATHWAY AND ESCRT MACHINERY IN THE TURNOVER OF THE AMYLOID PRECURSOR PROTEIN; IMPLICATIONS FOR AMYLOID-BETA GENERATION AND ALZHEIMER'S DISEASE.
BUSQUEDA DE ELEMENTOS MOLECULARES INVOLUCRADOS EN LA MAQUINARIA EXOCITICA DE PROTEINAS
ESTUDIO DE LA FUNCION DEL EJE HIPOTALAMO-HIPOFISIS-SUPRARRENAL Y DE LA PRODUCCION DE PROLACTINA POR CELULAS DEL SISTEMA INMUNE EN PACIENTES CON LUPUS ERITEMATOSO SISTEMICO Y ARTRITIS REUMATOIDEA.

Review (1)

Electron Tomography Revels that Milk Lipids Originate from Endoplasmic Reticulum Domains with Novel Structural Features
23
GONZALO MARDONES

Associate Professor

Physiology

Universidad Austral de Chile

Valdivia, Chile

8
PATRICIA BURGOS

Associate Professor & Sub-Director CEBICEM

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

4
Viviana Cavieres

Postdoctoral Researcher

Medicina y Ciencias

Universidad San Sebastián

Santiago , Chile

3
Andrea Soza

Profesor Asociado

Medicina y Ciencia

Universidad San Sebastián

Santiago, Chile

2
Luis González

Full Professor

School of Medicine

Universidad San Sebastián

Santiago, Chile

2
INGRID EHRENFELD

ASSOCIATE PROFESSOR

ANATOMIA; HISTOLOGIA & PATOLOGIA

UNIVERSIDAD AUSTRAL DE CHILE

VALDIVIA, Chile

1
Fanny Guzman

Investigador Senior

Núcleo de Biotecnología Curauma

Pontificia Universidad Católica de Valparaiso

Valparaiso, Chile

1
Hianara Bustamante

Profesor Adjunto Ad-Honorem

Instituto de Microbiología Clínica

Universidad Austral de Chile

Valdivia, Chile

1
Maria-Isabel Yuseff

Associate Professor

Cellular and Molecular Biology

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Fabiola Sánchez

Associate Profesor

Immunology

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Bredford Kerr

Profesor Asociado

CEBICEM

Universidad San Sebastián

Santiago, Chile

1
Gloria Arriagada

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Guillermo Valenzuela

Investigador Posdoctoral

Facultad de Medicina

Universidad Austral de Chile

Valdivia, Chile

1
Nibaldo Inestrosa

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Alejandro Rojas

Assistant Professor

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Claudia Yefi

Assistant professor

Medicina

Pontificia Universidad Católica de Chile

Santiago, Chile

1
CAROLA OTTH

profesor titular

microbiologia clinica

UNIVERSIDAD AUSTRAL DE CHILE. FACULTAD DE MEDICINA. INSTITUTO DE MICROBIOLOGIA CLINICA

VALDIVIA, Chile

1
Cheril Tapia

Profesor Asistente

Facultad de Medicina y Ciencia

Universidad San Sebastián

Santiago, Chile

1
Jorge Cancino

Assistant Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

1
Héctor Venegas

Profesor Titular asociado al Centro

Universidad Diego Portales

Santiago, Chile

1
Karina Cereceda

Investigador post doctoral

Diagnóstico

Fundación Arturo López Pérez

Providencia, Chile