Man

GONZALO ANTONIO MARDONES COFRE

Associate Professor

Universidad San Sebastián

Valdivia, Chile

Líneas de Investigación


Cell & Molecular Biology; Membrane trafficking; Breast cancer; Protein trafficking; Golgi structure and function; Protein crystallography; Structural biology; Biomolecular condensates; Evolutionary cell biology

Educación

  •  Cell & Molecular Biology, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 2003
  •  Biochemistry, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1995
  •  Bioquímico, UNIVERSIDAD AUSTRAL DE CHILE. Chile, 1997

Experiencia Académica

  •   Assistant Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2010 - 2017

  •   Associate Professor Full Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2019 - 2023

  •   Associate Professor Part Time

    Universidad San Sebastián

    Medicine & Science

    Santiago, Chile

    2017 - 2018

  •   Assistant Professor Part Time

    UNIVERSIDAD AUSTRAL DE CHILE

    Medicine

    Valdivia, Chile

    2017 - 2018

  •   Instructor Part Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    1998 - 1999

  •   Associate Professor Full Time

    Universidad San Sebastián

    Medicine & Science

    Valdivia, Chile

    2023 - A la fecha

Experiencia Profesional

  •   Research Fellow Full Time

    National Institutes of Health

    Bethesda, Estados Unidos

    2008 - 2010

  •   Postdoctoral Fellow Full Time

    National Institutes of Health

    Bethesda, Estados Unidos

    2005 - 2008

  •   Postdoctoral Fellow Full Time

    University of Colorado Health Sciences Center

    Denver, Estados Unidos

    2003 - 2005

  •   Assistant Professor Full Time

    Universidad Austral de Chile

    Valdivia, Chile

    2010 - 2019

  •   Associate Professor Full Time

    Universidad Austral de Chile

    Valdivia, Chile

    2020 - 2023

Formación de Capital Humano


2020 - Catalina Meléndez, Ph.D. Thesis student, Cell Biology & Biomedicine, Universidad San Sebastián. Efecto de GOLPH3 y su localización en el aparato de Golgi sobre la función mitocondrial y la agresividad tumoral en células de cáncer de mama. (Co-patrocinante).
2021 - 2023 Tammy Pástor, Undergraduate Thesis student, Biology, Yachay Tech University, Ecuador. "Caracterización evolutiva y funcional de la proteína GOLPH3 de mamíferos". (Tutor).
2021 - 2022 Abigail Galarza, Undergraduate Thesis student, Biotechnology, IKIAM Universidad Regional Amazónica, Ecuador. "Caracterización evolutiva y funcional de la proteína GOLPH3 de invertebrados". (Tutor).
2019 - 2021 Rodrigo Castro, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. "Validación y caracterización de la interacción entre la oncoproteína de Golgi GOLPH3 y la GTPasa RAB2A". (Patrocinante).
2017 - 2020 Viviana Cavieres, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. Rol de GOLPH3 en la función de lisosomas de células tumorales. (Patrocinante).
2016 - 2019 Charlotte Luchsinger, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. GOLPH3 promotes the tumor phenotype favoring chemoresistance and cell migration in T98G cells of glioblastoma multiforme. (Patrocinante).
2014 - 2017 Cecilia Arriagada, Ph.D. Thesis student, Cell & Molecular Biology, Universidad Austral de Chile. Participación de GOLPH3 en la vía EGFR/mTOR y su rol en el fenotipo tumoral de células T98G de glioblastoma multiforme. (Patrocinante).
2013 - 2015 Gustavo Leal, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Función de la degradación de proteínas asociado a retículo endoplásmico, en control de los niveles de la proteína precursora del amiloide. (Co-patrocinante).
2013 - 2015 María José Tenorio, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Análisis del comportamiento de GOLPH3 en células tumorales de glándula mamaria humana mediante fluorescence recovery after photobleaching (FRAP). (Patrocinante).
2012 - 2014 Yennyfer Arancibia, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Participación de la proteína de tegumento VP11/12 del HSV-1 en la alteración del aparato de Golgi y eventos neurodegenerativos tempranos. (Co-patrocinante).
2012 - 2013 Silvia Ojeda, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización de la interacción proteína-proteína de alfa-SNAP wild type y de su mutante M105I. (Co-patrocinante).
2012 - 2014 Rodrigo Oyarzún, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Estudios funcionales de mutantes del transportador facilitativo de glucosa Glut-1. (Co-patrocinante).
2011 - 2014 Diego Acuña, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Análisis preliminar del proteoma del aparato de Golgi de células tumorales MCF-7 de glándula mamaria humana. (Tutor).
2011 - 2012 Camilo Muñoz, Undergraduate Thesis student, Medical Technology, Universidad Austral de Chile. Estudio de las consecuencias de la mutación puntual M105I en la proteína alfa-SNAP en su capacidad de interacción con membrana plasmática. (Co-patrocinante).
2010 - 2013 Esteban Corales, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización de sitios de unión para señales de destinación en la subunidad ?2 del complejo adaptador de proteínas AP-2. (Tutor).
2010 - 2013 Breyan Ross, Undergraduate Thesis student, Biochemistry, Universidad Austral de Chile. Caracterización estructural y funcional de los sitios de unión a señales de tirosina del complejo adaptador AP-1A. (Tutor).


Premios y Distinciones

  •   Medalla Conmemorativa 2 de Octubre, Categoría Ciencias

    Gobierno Región de Los Ríos

    Chile, 2016

    Distinción anual otorgada por contribución a la Región de Los Ríos, Chile, en categoría Ciencias

  •   Honorable Mention Celldance 2007

    American Society for Cell Biology

    Estados Unidos, 2007

    Distinction for contribution to outreach in Cell Biology in form of videomicroscopy

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2007

    Postdoctoral fellowship

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2006

    Postdoctoral Fellowship

  •   Postdoctoral Visiting Fellowship, John E. Fogarty International Center

    NATIONAL INSTITUTE OF HEALTH

    Estados Unidos, 2008

    Postdoctoral Fellowship

  •   Travel Award

    American Society for Cell Biology

    Estados Unidos, 2001

    Predoctoral travel award to participate in the 41st Annual Meeting of the American Society for Cell Biology, December 8-12, Washington DC, USA

  •   Travel Award

    American Society for Biochemistry and Molecular Biology

    Estados Unidos, 2000

    Predoctoral travel award to participate in the Annual meeting of the American Society for Biochemistry and Molecular Biology, June 4-8, Boston, MA, USA

  •   Beca de doctorado

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 1997

    Beca para estudiantes de doctorado


 

Article (19)

How Many Sirtuin Genes Are Out There? Evolution of Sirtuin Genes in Vertebrates With a Description of a New Family Member
Identification of multiple TAR DNA Binding Protein retropseudogene lineages during the evolution of primates
Single-Fluorophore Indicator to Explore Cellular and Sub-cellular Lactate Dynamics
Independent duplications of the Golgi phosphoprotein 3 oncogene in birds
GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation
Human Golgi phosphoprotein 3 is an effector of RAB1A and RAB1B
Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded Forster Resonance Energy Transfer Indicator
Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded FRET Indicator
The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-to-ER Retrograde Transport
Galectin-8 induces endothelial hyperpermeability through the eNOS pathway involving S-nitrosylation-mediated adherens junction disassembly
KDEL receptor regulates secretion by lysosome relocation- and autophagy-dependent modulation of lipid-droplet turnover
Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling
Distinct Biochemical Pools of Golgi Phosphoprotein 3 in the Human Breast Cancer Cell Lines MCF7 and MDA-MB-231
Tetrahydrohyperforin Inhibits the Proteolytic Processing of Amyloid Precursor Protein and Enhances Its Degradation by Atg5-Dependent Autophagy
Analysis of Articulation Between Clathrin and Retromer in Retrograde Sorting on Early Endosomes
The trans-Golgi network accessory protein p56 promotes long-range movement of GGA/clathrin-containing transport carriers and lysosomal enzyme sorting
Selective plasma membrane permeabilization by freeze-thawing and immunofluorescence epitope access to determine the topology of intracellular membrane proteins
Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent
Hypothalamic-pituitary-adrenal axis function in patients with active rheumatoid arthritis: A controlled study using insulin hypoglycemia stress test and prolactin stimulation

Abstract (13)

Interaction of human GOLPH3 with RAB1A and RAB1B: identification of a putative non-canonical effector
Analysis of GOLPH3 knocking-down on protein glycosylation, trafficking and function in cells T98G of glioblastoma multiforme.
Identification of novel regulators of Amyloid Precursor Protein by High Content Imaging siRNA Screening
Defects in N-cadherin-based adherens junctions precede disruption of neural stem cells in alpha-SNAP mutant mice
Novel insights into the molecular mechanisms that mediated incorporation of the amyloid precursor protein into multivesicular bodies
Regulation of the endolysosomal pathway by beclin-1 and its role in the proteolytic processing of the amyloid precursor protein
Analysis of GOLPH3 depletion in human glioblastoma multiforme T98G cells
Depletion of Beclin-1 increases the delivery of cathepsin D and EGF receptor to lysosomes.
Endoplasmic reticulum-associated degradation controls the levels of amyloid precursor protein
Depletion of Beclin-1 reduces the levels of carboxy terminal fragment beta of the amyloid precursor protein by a mechanism dependent on autophagosome function
Galectin-8 increases cell proliferation activating Sonic Hedgehog and EGFR Pathways
APIB adaptor complex mediates basolateral sorting in a post-TGN compartment at the crossroads of the biosynthetic and recycling pathways
An autoantigen contained in a cytosolic protein complex associates with the trans-Golgi network (TGN)

Errata (1)

Publisher Correction: ?-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility (Scientific Reports, (2017), 7, 1, (11765), 10.1038/s41598-017-12292-9)

Proyecto (3)

Evolution of the N-terminal region of the Golgi phosphoprotein 3 (GOLPH3) oncoprotein
Partitioning of the oncoprotein GOLPH3 in biomolecular condensates by liquid-liquid phase separation: a mechanism for the phenotypic plasticity of breast cancer cells
The interplay between phosphorylation and ubiquitylation controls stabilization of HERPUD1 under ER stress: possible implications on TNBC aggressiveness

Review (1)

Electron Tomography Revels that Milk Lipids Originate from Endoplasmic Reticulum Domains with Novel Structural Features
1
CAROLA OTTH

profesor titular

microbiologia clinica

UNIVERSIDAD AUSTRAL DE CHILE. FACULTAD DE MEDICINA. INSTITUTO DE MICROBIOLOGIA CLINICA

VALDIVIA, Chile

1
Bredford Kerr

Profesor Asociado

CEBICEM

Universidad San Sebastián

Santiago, Chile

3
Claudio Hetz

Deputy Director

INSTITUTO DE CIENCIAS BIOMÉDICAS, FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Fanny Guzman

Investigador Senior

Núcleo de Biotecnología Curauma

Pontificia Universidad Católica de Valparaiso

Valparaiso, Chile

1
Juan Opazo

Profesor Titular

Facultad de Medicina y Ciencia

Universidad San Sebastián

Valdivia, Chile

10
Patricia Burgos

Associate Professor Programa de Doctorado en Biología Celular y Biomedicina CEBICEM

UNIVERSIDAD SAN SEBASTIÁN

Santiago, Chile

39
GONZALO MARDONES

Associate Professor

Medicine

Universidad San Sebastián

Valdivia, Chile

1
Fabiola Sánchez

Associate Profesor

Immunology

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

3
Luis González

Full Professor

School of Medicine

Universidad San Sebastián

Santiago, Chile

3
Andrea Soza

Profesor Asociado

Medicina y Ciencia

Universidad San Sebastián

Santiago, Chile

1
Nibaldo Inestrosa

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Héctor Venegas

Profesor Titular asociado al Centro

Universidad Diego Portales

Santiago, Chile

1
Francisco Morera

Assistant Professor

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Gloria Arriagada

Profesor Asociado-Investigador Principal

Instituto de Ciencias Biomédicas

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

1
Luis Federico Batiz

Full Professor

Center for Biomedical Research and Innovation (CIIB)

Universidad de Los Andes

Santiago, Chile

1
Maria-Isabel Yuseff

Associate Professor

Cellular and Molecular Biology

Pontificia Universidad Católica de Chile

Santiago, Chile

2
INGRID EHRENFELD

FULL PROFESSOR

ANATOMIA; HISTOLOGIA & PATOLOGIA

UNIVERSIDAD AUSTRAL DE CHILE

VALDIVIA, Chile

1
Luis Vargas

Full Professor

Universidad Austral de Chile

Valdivia, Chile

1
Alejandro Rojas

Assistant Professor

UNIVERSIDAD AUSTRAL DE CHILE

Valdivia, Chile

1
Ivan Ruminot

INVESTIGADOR

BIOLOGIA

CENTRO DE ESTUDIOS CIENTIFICOS

VALDIVIA, Chile

1
Cheril Tapia

Profesor Asistente

Facultad de Medicina y Ciencia

Universidad San Sebastián

Santiago, Chile

1
Claudia Yefi

Assistant professor

Medicina

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Jorge Cancino

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

1
María Miró

Académico

Facultad de Salud

Universidad Santo Tomás

Valdivia, Chile

3
Hianara Bustamante

Profesora Auxiliar

Instituto de Microbiología Clínica

Universidad Austral de Chile

Valdivia, Chile

1
Karina Cereceda

Investigador post doctoral

Diagnóstico

Fundación Arturo López Pérez

Providencia, Chile

1
Guillermo Valenzuela

Docente Investigador

Facultad de Ciencias para el cuidado de la Salud

Universidad San Sebastián

Valdivia, Chile

4
Viviana Cavieres

Postdoctoral Researcher

Medicina y Ciencias

Universidad San Sebastián

Santiago , Chile

1
CECILIA ARRIAGADA

Postdoctoral Fellow

Rutgers University

Newark, Estados Unidos

1
ALEX GALAZ

COFOUNDER (Co-CEO)

INFOOD PROTEIN AND BIOTECHNOLOGY

VALDIVIA, Chile

1
Diego Tapia

Asistente de Investigación

Universidad San Sebastián

Santiago, Chile

1
Angelo Bernier

Tesista de Magíster

Biología

Centro de Estudios Científicos de Valdivia

Valdivia, Chile

1
Camila Aburto

PhD(c)

Biología

Centro de estudios Científicos

Valdivia, Chile

1
Javier Badilla

Estudiante Doctorado

Nucleo Biotecnologia Curauma

Pontificia Universidad Católica de Valparaíso

Valparaiso, Chile