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Tatiana Marlene Adasme Rocha

Associate Researcher

Universidad Chile

Santiago, Chile

Líneas de Investigación


Synaptic plasticity and Memory; Calcium Signals; Redox signaling; Calcium and Redox regulated Transcriptional Factors; Signal transduction in Aging

Educación

  •  Biochemist, UNIVERSIDAD DE CHILE. Chile, 2004
  •  Biochemist bachelor, UNIVERSIDAD DE CHILE. Chile, 2004
  •  Doctor in Biochemistry, UNIVERSIDAD DE CHILE. Chile, 2009

Experiencia Académica

  •   Teacher and coordinator Part Time

    Universidad de Artes, Ciencias y Comunicación (UNIACC)

    Santiago, Chile

    2014 - 2015

  •   Physiology teacher Part Time

    Universidad San Sebastian

    Santiago, Chile

    2009 - 2010

  •   Physiology teacher Part Time

    Universidad Autónoma

    Santiago, Chile

    2010 - 2010

  •   Physiology teacher Full Time

    Universidad Bernardo O´Higgins

    Facultad de Ingeniería, Ciencias y Tecnología

    Santiago, Chile

    2017 - 2019

  •   Physiology teacher Full Time

    Universidad de Bernardo O´Higgins

    Health

    Santiago, Chile

    2016 - 2020

  •   Physiology teacher Part Time

    UNIVERSIDAD DE VALPARAISO

    Facultad de Medicina

    Santiago, Chile

    2006 - 2006

  •   teacher for Intracellular Calcium signals course Part Time

    UNIVERSIDAD DE CHILE

    Facultad de Medicina

    Santiago , Chile

    2007 - 2008

  •   Teacher for Biomedicine I and II (this course is lectured each 2 years) Part Time

    UNIVERSIDAD DE CHILE

    Facultad de Medicina

    Santiago, Chile

    2014 - 2020

  •   Teacher and evaluator of Research Units Other

    UNIVERSIDAD DE CHILE

    Facultad de Medicina

    Santiago, Chile

    2012 - 2017

  •   teacher Part Time

    UNIVERSIDAD DE CHILE

    Medicine

    Santiago, Chile

    2018 - 2019

  •   Physiology teacher Full Time

    Universidad Bernardo O´Higgins

    Health

    Santiago, Chile

    2016 - 2020

  •   laboratory teacher Part Time

    UNIVERSIDAD DE CHILE

    Medicine

    Santiago, Chile

    2019 - 2019

  •   teacher Full Time

    UNIVERSIDAD BERNARDO O'HIGGINS

    Sciences of health

    Santiago, Chile

    2019 - 2020

  •   teacher Full Time

    UNIVERSIDAD BERNARDO O'HIGGINS

    Health

    santiago, Chile

    2020 - 2020

  •   Cellular and genetical Biology teacher Part Time

    UNIVERSIDAD BERNARDO O'HIGGINS

    Health

    Santiago, Chile

    2020 - 2020

Experiencia Profesional

  •   Researcher, FONDAP/CEMC Fellowship Full Time

    Universidad de Chile/Facultad de medicina/ Laboratorio de Señales de Calcio

    Santiago, Chile

    2009 - 2011

  •   postdegree CONICYT fellowship Full Time

    Universidad de Chile/Facultad de medicina/ Laboratorio de Señales de Calcio

    Santiago, Chile

    2006 - 2009

  •   Member of professional team, FONDECYT 1050768 Part Time

    Universidad de Chile/Facultad de Ciencias Químicas y Farmacéuticas/ Laboratorio de Transducción de señales moleculares de la célula

    Santiago, Chile

    2005 - 2006

  •   member of Professional team proyecto FONDECYT 1050690 Part Time

    Universidad de los Andes/ Laboratorio de Bioquímica y Fisiología celular

    Santiago, Chile

    2004 - 2005

  •   undergraduate in professional thesis Full Time

    Universidad de Chile/Facultad de Ciencias Químicas y Farmaceúticas/ Laboratorio de Transducción de señales

    Santiago, Chile

    2002 - 2004

  •   student in laboratory Part Time

    Universidad de Chile/Facultad de Ciencias/ Laboratorio de Bioquímica

    Santiago, Chile

    1999 - 2000

  •   Investigadora CIBQA Full Time

    Universidad Bernardo O´Higgins

    Santiago, Chile

    2016 - 2020

  •   BNI researcher in collaboration Part Time

    Universidad de Chile/Facultad de medicina/ Laboratorio de Señales de Calcio

    Santiago, Chile

    2014 - A la fecha

  •   Adjunct Professor, Faculty of Medicine Part Time

    Universidad de Chile

    Santiago, Chile

    2019 - 2019

  •   advisory committee Other

    ANID/FONDEQUIP

    Santiago, Chile

    2019 - A la fecha

  •   Evaluadora Panel Instrumentos Bionalíticos Part Time

    ANID/FONDEQUIP

    Santiago, Chile

    2018 - 2018

  •   Coordinadora Clinica Part Time

    Clinica Psiquiatrica/HCUCH

    Santiago, Chile

    2022 - A la fecha

Formación de Capital Humano


Training of Young Investigators
1. Undergraduated Thesis: Italo Espinoza Fuenzalida; “The field electrical stimulation modulates the RyR2 expression in primary hippocampal neurons”; 2007-2010; Universidad de Chile; CHILE; Thesis for to be Biochemist. Function: co-director; director: Dra. María Angélica Carrasco. Laboratorio de Bioquímica Celular, Facultad de Medicina, Universidad de Chile.

2. Undergraduated Thesis: Karla Cataldo, Patricio Farías; Daniela Meza; “Efecto de la Astaxantina sobre la translocación del Factor Nuclear kappa B en hipocampo de ratas envejecidas y en células PC12”; Universidad de Valparaíso; CHILE; Thesis for to be Medical Technologist. Function: co-advisor; advisor: Dr. Pablo Munoz. Laboratory of Cellular and Molecular Plasticity, Facultad de Medicina, Universidad de Valparaíso.

3. Magister Thesis: Bábara Bruna Jara; “Involvement of the transcription factor Nrf2 in the increases of RyR2 expression induced of BDNF.”; 2015-2017; Universidad de Chile; CHILE; Master in Biochemistry Thesis. Function: co-director; Director: Dra. Andrea Paula-Lima. Laboratory of Celular and Molecular Biology, Faculty of Dentistry, Universidad de Chile.
- Professional trainee: Italo Espinoza Fuenzalida; “Detection of peroxide in hippocampal primary neurons stimulated electrically” Function: Chief professor. Laboratory of Celular Biochemistry, Faculty of Medicine, Universidad de Chile.

4. Magister Thesis: Marilyn Fonseca; “ Effects of beta-amyloid oligomers on Nrf2 nuclear translocation and expression of antioxidant enzymes in primary hippocampal neurons”; 2018 – in course; Universidad Bernardo O´Higgins; CHILE; Master in Chemical and Biological Sciences. Function: Advisor; co-advisor: Dra. Andrea Paula-Lima. Laboratory of Cellular and Molecular Biology, Faculty of Dentistry, Universidad de Chile.

Early scientific training for undergraduate and post degree students.

1. 2012-2019: 19 Undergraduate students, Medicine School (second year), Faculty of Medicine, Universidad de Chile. Function: Tutor. Laboratory of Calcium Signals, Faculty of Medicine, Universidad de Chile.
2. 2017-2017: 1 Postdegree student, Pharmacology program of PhD, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile.
2. 2008-2017: 8 Undergraduate students, Biochemistry School (second year), Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile.
3. 2012: 1 Undergraduate student, Pharmacy and Chemistry School (4Th), Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile.

Committee member in Post degree Programs

- 2019-2020 - Member of the Master thesis evaluation committee. "Inhibition of ryanodine receptors prevents presynaptic changes induced by LTD" Tatiana Guncay, Master in Medical Sciences, Cellular and Molecular Biology, Universidad de Valparaíso.
- 2019- present – Member of Doctorate thesis evaluation committee. “Pannexin-1 regulates dendrite branching and dendritic spines formation in hippocampal neurons by modulating actin polymerization through Rho GTPases.” Carolina Flores, PhD in Sciences, Neurosciences.


Difusión y Transferencia


1. Project for development of critical and scientific thinking in students of middle education at Instituto Miguel León Prado. October-December 2015 and 2018.

2. Internship at the Institute of Neurobiology of Magdeburg, Germany, Leibniz Institute for Neurobiology (LIN), preparation of joint research project with Dr. Rodrigo Herrera Molina, Co-leader of the group in the Department of Neurochemistry and Molecular Biology of LIN . August 29 - September 13, 2017.

3. Project for the development of critical and scientific thinking for students of middle education at Instituto Miguel León Prado. October-December 2018.

4. Project for development of critical and scientific thinking for pre-basic and middle education students at Instituto Miguel León Prado. October-December 2015.
5. EXPLORA CONICYT; guide professor, Universidad de Chile, Open house Cicle 2008.

6. EXPLORA CONICYT: Cientific Committee evaluator, second stage of selected works evaluation, 8th School Meeting of Science and technology, EXPLORA CONICYT, Región Metropolitana, Universidad Católica de Chile, October 2013.


Premios y Distinciones

  •   Doctoral thesis fellowship AT-24080073

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 2008

    Fellowship for the performing of doctoral thesis (complement to D-21060628) from CONICYT (Comisión Nacional de Investigación Científica y Tecnológica), AT-24080073, Gobierno de Chile, (2008).

  •   Doctoral Fellowship, CONICYT D-21060628

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 2006

    CONICYT (Comisión Nacional de Investigación Científica y Tecnológica) Doctoral fellowship, D-21060628, Gobierno de Chile (2006-2009).

  •   Fellowship for Scientific Meeting assistance

    UNIVERSIDAD DE CHILE

    Chile, 2007

    Fellowship from Universidad de Chile for present and participate to the Course entitled “Neurochemical & Cellular Mechanism of Neuronal Damage. Therapeutical approaches”, Marzo 22-25, Pucón, Chile, 2007.

  •   Brazilian Academy of Sciencies 13rd TWAS-ROLAC

    The World Academy of Science

    Chile, 2013

    Awarded with fellowship to travel and preset my research (poster session) in “13rd TWAS-ROLAC Young Scientists Conference ”. Río de Janeiro, 6-8 de Mayo, 2013.

  •   Chilean Neuroscience society fellowship

    Chilean Society of Neuroscience

    Chile, 2013

    Awarded for travel and present my postdegree research in the session of Young Neuriscientist meeting, " IX Annual Meeting of Chilean Neurosciente Society and VIII Meeting of Latinoamerican Biophysics. Valparaíso, 1-4 de Octubre, 2013, Chile.

  •   FONDEQUIP Technical committee member

    COMISION NACIONAL DE INVESTIGACION CIENTIFICA Y TECNOLOGICA

    Chile, 2019

    Member of national committee of Fund of scientific and technologic equipment from January 2019.

  •   Member of Group III: Bioanalitical Instruments Evaluator

    FONDEQUIP

    Chile, 2018

    Member of evaluation pannel.


 

Article (19)

RyR-mediated Ca2+ release elicited by neuronal activity induces nuclear Ca2+ signals, CREB phosphorylation , and Npas4/RyR2 expression
Astaxanthin Counteracts Excitotoxicity and Reduces the Ensuing Increases in Calcium Levels and Mitochondrial Reactive Oxygen Species Generation
Calcium Release Mediated by Redox-Sensitive RyR2 Channels Has a Central Role in Hippocampal Structural Plasticity and Spatial Memory
The signaling pathways underlying BDNF-induced Nrf2 hippocampal nuclear translocation involve ROS, RyR-Mediated Ca2+ signals, ERK and PI3K
Triclosan Impairs Hippocampal Synaptic Plasticity and Spatial Memory in Male Rats
Aging Impairs Hippocampal-Dependent Recognition Memory and LTP and Prevents the Associated RyR Up-regulation
Development of an iron-selective antioxidant probe with protective effects on neuronal function
RyR2-Mediated Ca2+ Release and Mitochondrial ROS Generation Partake in the Synaptic Dysfunction Caused by Amyloid beta Peptide Oligomers
Astaxanthin protects primary hippocampal neurons against noxious effects of A ?-oligomers
Inhibitory ryanodine prevents ryanodine receptor-mediated Ca2+ release without affecting endoplasmic reticulum Ca2+ content in primary hippocampal neurons
The Antioxidant N-Acetylcysteine Prevents the Mitochondrial Fragmentation Induced by Soluble Amyloid-beta Peptide Oligomers
Amyloid beta-Peptide Oligomers Stimulate RyR-Mediated Ca2+ Release Inducing Mitochondrial Fragmentation in Hippocampal Neurons and Prevent RyR-Mediated Dendritic Spine Remodeling Produced by BDNF
High-Frequency Field Stimulation of Primary Neurons Enhances Ryanodine Receptor-Mediated Ca2+ Release and Generates Hydrogen Peroxide, Which Jointly Stimulate NF-kappa B Activity
Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity and spatial memory formation
Increased Hippocampal Expression of the Divalent Metal Transporter 1 (DMT1) mRNA Variants 1B and +IRE and DMT1 Protein After NMDA-Receptor Stimulation or Spatial Memory Training
Increased Hippocampal Expression of the Divalent Metal Transporter 1 (DMT1) mRNA Variants 1B and +IRE and DMT1 Protein After NMDA-Receptor Stimulation or Spatial Memory Training
The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling
The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.
IGF-1 protects cardiac myocytes from hyperosmotic stress-induced apoptosis via CREB.

ConferencePoster (9)

BDNF through RyR-mediated Ca2+ release and ROS signaling induces Nrf2 translocation in hippocampal neurons.
BDNF-induced hippocampal structural plasticity requires ROS-stimulated RyR2-mediated Ca2+ release.
Ryanodine receptor mediated calcium signals and ROS jointly mediate BDNF-induced Nrf2 nuclear translocation in primary hippocampal neurons.
“Anomalous Calcium/Redox signaling in rats exposed to amyloid b-oligomers: Function and behavioral correlates with early Alzheimer patients.”
“Ca2+ and ROS participate in BDNF signaling: a novel role for Nrf2”
“Calcium signals evoked in dendrites of primary rat hippocampal neurons engage RyR2- mediated calcium reléase for propagation to the nucleus.”
“RyR2-mediated Calcium release has a key role in hippocampal synaptic plasticity and memory processes and its defective in a rat model of Alzheimer disease.”
BDNF increase RyR2 expression through Ca2+ and ROS signaling induced Nrf2 translocation in hippocampal neurons.
Neuronal structural plasticity and LTP induction require Ryanodine Receptor mediated Ca2+ release.

Proyecto (12)

Unveiling how neuronal calcium signals reach the nucleus following synapse activation
Hippocampal Ryanodine ReceptorsCalcium Release Channels Mediate Activity-Generated Postsynaptic Calcium Signals and their Propagation to the Nucleus
Hippocampal Ryanodine Receptors Calcium Release Channels Mediate Activity Generated Postsynaptic Calcium Signals and their Propagation to the Nucleus
“Calcium- and ROS-signaling, key cellular pathways underlying BDNF induced plasticity”
“Calcium- and ROS-signaling, key cellular pathways underlying BDNF induces plasticity”
“Participación de la liberación de calcio mediada por RyR en respuesta a BDNF en la activación de cascadas de señales que inducen la expresión de proteínas esenciales para la plasticidad sináptica y memoria espacial”.
PARTICIPACION DE LA LIBERACION DE CALCIO MEDIADA POR RYR EN RESPUESTA A BDNF EN LA ACTIVACION DE CASCADAS DE SEÑALES QUE INDUCEN LA EXPRESION DE PROTEINAS ESENCIALES PARA LA PLASTICIDAD SINAPTICA Y MEMORIA ESPACIAL.
PARTIAL DIFFERENTIAL EQUATIONS FOR 3D PHOTON DENOISING, OPTICAL FLOW AND ADJACENT ACTIVE SURFACE MODELS FOR HIGH THROUGHPUT IN VIVO SPINNING DISK MICROSCOPY
PARTIAL DIFFERENTIAL EQUATIONS FOR 3D PHOTON DENOISING, OPTICAL FLOW AND ADJACENT ACTIVE SURFACE MODELS FOR HIGH THROUGHPUT IN VIVO SPINNING DISK MICROSCOPY
Partial differential equations for 3D photon denoising, optical flow and adjacent active surface models for high throughput in vivo spinning disk microscopy
“Modulacion redox de reguladores transcripcionales en neuronas de hipocampo”.
MECANISMOS DE TRANSDUCCION DE SENALES DEL IGF-1=>REGULACION DIFERENCIAL DE FACTORES TRANSCRIPCIONALES ASOCIADOS A LOS PROCESOS DE HIPERTROFIA Y APOPTOSIS DEL CARDIOMIOCITO

Review (1)

Contribution of Ca2+ Release Channels to Hippocampal Synaptic Plasticity and Spatial Memory: Potential Redox Modulation
31
Tatiana Adasme

Associate Researcher

Centro de Investigación Clínica Avanzada (CICA)

Universidad Chile

Santiago, Chile

10
M. Cecilia Hidalgo

Professor

ICBM

Universidad de Chile

Santiago, Chile

9
Andrea Paula-Lima

Assistant Professor

Institute for research in Dental Sciences

Universidad de Chile

Santiago, Chile

4
Barbara Bruna

Asistente de Investigación

Hospital Clinico universidad de Chile-Universidad de Chile

Santiago, Chile

3
Gina Sanchez

Profesor Asociado

ICBM

UNIVERSIDAD DE CHILE, FAC MEDICINA , ICBM

Santiago, Chile

3
José Galaz

Estudiante de Posgrado

Instituto de Investigación en Ciencias Odontológicas

Universidad de Chile

Santiago, Chile

2
Rodrigo Herrera

Group Leader (staff member)

Combinatorial NeuroImaging Core Facility

Leibniz Institute for Neurobiology

Magdeburg, Alemania

2
Italo Espinoza

Lab Manager

Centro de Biología Integrativa

Universidad Mayor de Chile

Santiago, Chile

2
Carol San Martín

Profesora Asistente

Universidad Mayor de Chile

Santiago, Chile

2
jose valdes

Profesor Asociado

Neurociencia

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Jamileth More

Investigador

Neurologia

Hospital Clinico Universidad de Chile

Santiago, Chile

2
Genaro Barrientos

Profesor Asistente

Fisiologia y Biofisica, ICBM

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Andrea Paula-Lima

Profesora Asociada

Instituto de Investigación en Ciencias Odontológicas

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Steffen Härtel

Full Professor

Instituto de Ciencias Biomédicas

University of Chile

Santiago, Chile

2
Marco Nuñez

Professor

Biology

Fac. Ciencias, Universidad de Chile

Santiago, Chile

2
PAOLA HAEGER

Associated professor

Ciencias Biomedicas

Universidad Catolica del Norte

COQUIMBO, Chile

1
Juan Valdés

Head lab

Ciencias Biologicas

UNIVERSIDAD ANDRÉS BELLO

Santiago, Chile

1
Pablo Muñoz

UNIVERSIDAD DE VALPARAÍSO

Valparaíso, Chile

1
Mario Chiong

Profesor

Departamento de Bioquímica y Biología Molecular

Universidad de Chile

Santiago, Chile

1
Leticia Gonzalez

Profesional Apoyo a la Investigacion

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Roberto Bravo

Assistant Professor

Institute of Nutrition and Food Technology (INTA), Universidad de Chile

Santiago, Chile

1
Denise Riquelme

Adjunct Professor

Biology

Universidad de Santiago

Santiago, Chile

1
ALEXIS HUMERES

DIRECTOR

MORFOFUNCIÓN

Universidad Diego Portales

SANTIAGO, Chile

1
Sergio Lavandero

Director

Advanced Center for Chronic Diseases (ACCDiS)

UNIVERSIDAD DE CHILE, FAC CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

1
Felipe Oyarzún

Profesor Asociado

Ciencias y Tecnología Farmacéuticas

UNIVERSIDAD DE CHILE

Santiago, Chile