Man

Danilo Bilches Medinas

Staff Scientist

Instituto de Neurociencia Biomedica

Santiago, Chile

Líneas de Investigación


Free Radicals and Oxidants; Protein Misfolding and Aggregation; Cellular Stress; Cell Signaling; Neurodegenerative Diseases; Neurodevelopmental Disorders; Gene Therapy.

Educación

  •  Biochemistry, UNIVERSIDADE DE SAO PAULO. Brasil, 2010
  •  Chemistry, UNIVERSIDADE DE SAO PAULO. Brasil, 2005

Experiencia Académica

  •   Postdoctoral Fellow Full Time

    STANFORD UNIVERSITY

    Palo Alto, Estados Unidos

    2010 - 2011

  •   postdoc Full Time

    UNIVERSIDAD DE CHILE

    Santiago, Chile

    2012 - 2015

Experiencia Profesional

  •   Staff Scientist Full Time

    Instituto de Neurociencia Biomedica

    Santiago, Chile

    2015 - A la fecha

Formación de Capital Humano


1. Giselle Aimé Espinosa Vásquez, Evaluation of the impact of conditional genetic ablation of Pdia3 on the cognitive capacity of mice. Director. Undergraduate Thesis in Biochemistry. University of Santiago de Chile. Ongoing.
2. José Vicente González Teuber, Study of the contribution of IRE1? signaling to experimental amyotrophic lateral sclerosis (ALS). Co-director. PhD Thesis in Biomedical Sciences. University of Chile. Ongoing.
3. Pablo Sebastián Rozas Rojas, Effects of Erp57 Overexpression in a Mutant Sod1 Mouse Model of Amyotrophic Lateral Sclerosis. Co-director. Master Thesis in Biological Sciences. University of Chile. 2017.
4. Martín Nicolás Sepúlveda Durand, Characterization of Transgenic Mouse Lines for Mutant Forms of ERp57 Associated to Amyotrophic Lateral Sclerosis. Co-director. Undergraduate Thesis of Molecular Biotechnology Engineering. University of Chile. 2015.


Premios y Distinciones

  •   Travel Award

    Society for Free Radical Biology and Medicine

    Uruguay, 2007

    Travel Award from the Society for Free Radical Biology and Medicine to present the work entitled “An Alternative Mechanism for Carbonate Radical Generation during Cu,ZnSod Peroxidase Activity”.

  •   Travel Award

    Society for Free Radical Biology and Medicine

    Estados Unidos, 2009

    Travel Award from the Society for Free Radical Biology and Medicine to present the work entitled “MS Characterization of the Human Sod1 Covalent Dimer Produced from the Enzyme Peroxidase Activity. Implication to ALS”.

  •   Dean’s Fellowship

    STANFORD UNIVERSITY

    Chile, 2010

    Postdoctoral Fellowship

  •   FAPESP Fellowship

    FAPESP

    Brasil, 2005

    Doctorate Fellowship


 

Article (23)

beta-catenin aggregation in models of ALS motor neurons: GSK3 beta inhibition effect and neuronal differentiation

Pinto C.; Medinas D.B.; Fuentes-Villalobos F.; Maripillán J.; Castro A.F.; Martínez A.D.; Osses N.; Hetz C.; Henríquez J.P.

Article ISI SCOPUS
NEUROBIOLOGY OF DISEASE  (2019)

Network approach identifies Pacer as an autophagy protein involved in ALS pathogenesis

Beltran, S; Nassif M.; Vicencio E.; Arcos J.; Labrador L.; Cortes B.I.; Cortez C.; Bergmann C.A.; Espinoza S.; Hernandez M.F.; Matamala, J. M.; Bargsted L.; Matus S.; Rojas-Rivera D.; Bertrand M.J.M.; Medinas D.B.; Hetz C.; Manque P.A.; Woehlbier U.

Article ISI SCOPUS
MOLECULAR NEURODEGENERATION  (2019)

Endoplasmic reticulum stress leads to accumulation of wild-type SOD1 aggregates associated with sporadic amyotrophic lateral sclerosis.

Medinas, Danilo B; Rozas, Pablo; Martínez Traub, Francisca; Woehlbier, Ute; Brown, Robert H; Bosco, Daryl A; Hetz, Claudio

Article
Proceedings of the National Academy of Sciences of the United States of America  (2018)

Disulfide cross-linked multimers of TDP-43 and spinal motoneuron loss in a TDP-43(A315T) ALS/FTD mouse model

Bargsted, L; Medinas, DB; Traub, FM; Rozas, P; Muñoz, N; Nassif, M; Jerez, C; Catenaccio, A; Court, FA; hetz, C; Matus, S

Article ISI SCOPUS
SCIENTIFIC REPORTS  (2017)

Fine-Tuning ER Stress Signal Transducers to Treat Amyotrophic Lateral Sclerosis

Medinas D.B.; González J.V.; Falcon P.; Hetz C.

Article ISI SCOPUS
FRONTIERS IN MOLECULAR NEUROSCIENCE  (2017)

IRE1 signaling exacerbates Alzheimer's disease pathogenesis

Duran-Aniotz, Claudia; Cornejo, Victor Hugo; Espinoza, Sandra; Ardiles, Álvaro O.; Medinas, Danilo B.; Salazar, Claudia; Foley, Andrew; Gajardo, Ivana; Thielen, Peter; Iwawaki, Takao; Scheper, Wiep; Soto, Claudio; Palacios, Adrian G.; Hoozemans, Jeroen J. M.; Hetz, Claudio

Article ISI SCOPUS
ACTA NEUROPATHOLOGICA  (2017)

Adaptive preconditioning in neurological diseases - therapeutic insights from proteostatic perturbations

Mollereau, B; Rzechorzek, NM; Roussel, BD; Sedru, M; Van den Brink, DM; Bailly-Maitre, B; Palladino, F; Medinas, DB; Domingos, PM; Hunot, S; Chandran, S; Birman, S; Baron, T; Vivien, D; Duarte, CB; Ryoo, HD; Steller, H; Urano, F; Chevet, E; Kroemer, G; Ciechanover, A; Calabrese, EJ; Kaufman, RJ; hetz, C

Article ISI SCOPUS
BRAIN RESEARCH  (2016)

ALS-linked protein disulfide isomerase variants cause motor dysfunction

Woehlbier, U; Colombo, A; Saaranen M.J.; Pérez V; Ojeda J.; Bustos F.J.; Andreu C.I.; Torres M.; Valenzuela, V.; Medinas D.B.; Rozas P.; Vidal R.L.; Lopez-Gonzalez R.; Salameh J.; Fernandez-Collemann S.; Muñoz, N.; Matus, S; Armisen, R; Sagredo A; Palma K.; Irrazabal T.; Almeida, S.; Gonzalez-Perez P.; Campero, M; Gao F.-B.; Henny, P; van Zundert, B; Ruddock L.W.; Concha, M. L.; Henríquez J.P.; Brown R.H.; hetz C.

Article ISI SCOPUS
EMBO JOURNAL  (2016)

Creutzfeldt-Jakob disease. Report of one case

Ramirez, M; Gallardo, A; Vidal, A; Cornejo, S; Ramirez, D; Medinas, D; Bustamante, G; Pasquali, R; hetz, C

Article ISI
REVISTA MEDICA DE CHILE  (2016)

Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration

Castillo, Valentina; Oate, Maritza; Woehlbier, Ute*; Rozas, Pablo; Andreu, Catherine; Medinas, Danilo; Valds, Pamela; Osorio, Fabiola; Mercado, Gabriela; Vidal, René L.; Kerr, Bredford; Court, Felipe A.; Hetz, Claudio; Gillingwater, Thomas H

Article ISI SCOPUS
PLOS ONE  (2015)

Oligomerization of Cu,Zn-Superoxide Dismutase (SOD1) by Docosahexaenoic Acid and Its Hydroperoxides In Vitro=> Aggregation Dependence on Fatty Acid Unsaturation and Thiols.

Appolinário, Patricia Postilione; Medinas, Danilo Bilches; Chaves-Filho, Adriano B; Genaro-Mattos, Thiago C; Cussiol, José Renato Rosa; Netto, Luis Eduardo Soares; Augusto, Ohara; Miyamoto, Sayuri

Article
PloS one  (2015)

The Protein-disulfide Isomerase ERp57 Regulates the Steady-state Levels of the Prion Protein.

Torres, Mauricio; Medinas, Danilo B; Matamala, José Manuel; Woehlbier, Ute; Cornejo, Víctor Hugo; Solda, Tatiana; Andreu, Catherine; Rozas, Pablo; Matus, Soledad; Muñoz, Natalia; Vergara, Carmen; Cartier, Luis; Soto, Claudio; Molinari, Maurizio; Hetz, Claudio

Article
The Journal of biological chemistry  (2015)

Common ground: stem cell approaches find shared pathways underlying ALS.

Matus, Soledad; Medinas, Danilo B; Hetz, Claudio

Article
Cell stem cell  (2014)

Protein homeostasis: Modeling UPR adaptive responses.

Medinas, Danilo B; Hetz, Claudio

Article
Nature chemical biology  (2014)

The transcription factor CHOP, a central component of the transcriptional regulatory network induced upon CCl4 intoxication in mouse liver, is not a critical mediator of hepatotoxicity.

Campos, Gisela; Schmidt-Heck, Wolfgang; Ghallab, Ahmed; Rochlitz, Katharina; Pütter, Larissa; Medinas, Danilo B; Hetz, Claudio; Widera, Agata; Cadenas, Cristina; Begher-Tibbe, Brigitte; Reif, Raymond; Günther, Georgia; Sachinidis, Agapios; Hengstler, Jan G; Godoy, Patricio

Article
Archives of toxicology  (2014)

ER Dysfunction and Protein Folding Stress in ALS.

Matus, Soledad; Valenzuela, Vicente; Medinas, Danilo B; Hetz, Claudio

Article
International journal of cell biology  (2013)

Proteostasis impairment: at the intersection between Alzheimer's disease and diabetes.

Medinas, Danilo B; Hetz, Claudio

Article
Cell metabolism  (2013)

Generation of singlet oxygen by the glyoxal-peroxynitrite system.

Massari, Júlio; Tokikawa, Rita; Medinas, Danilo B; Angeli, José P F; Di Mascio, Paolo; Assunção, Nilson A; Bechara, Etelvino J H

Article
Journal of the American Chemical Society  (2011)

A ditryptophan cross-link is responsible for the covalent dimerization of human superoxide dismutase 1 during its bicarbonate-dependent peroxidase activity.

Medinas, Danilo B; Gozzo, Fabio C; Santos, Luiz F A; Iglesias, Amadeu H; Augusto, Ohara

Article
Free radical biology & medicine  (2010)

Mechanism of the peroxidase activity of superoxide dismutase 1.

Medinas, Danilo B; Augusto, Ohara

Article
Free radical biology & medicine  (2010)

Peroxymonocarbonate and Carbonate Radical Displace the Hydroxyl-like Oxidant in the Sod1 Peroxidase Activity under Physiological Conditions

Augusto, Ohara; do-Amaral, Antonia T.; Medinas, Danilo B.; de-Rezende, Leandro; Cerchiaro, Giselle; Malvezzi, Alberto; Toledo, Jose C., Jr.

Article ISI
CHEMICAL RESEARCH IN TOXICOLOGY  (2009)

The carbonate radical and related oxidants derived from bicarbonate buffer

Augusto, Ohara; Trindade, Daniel F.; Medinas, Danilo B.; Cerchiaro, Giselle

Article ISI
IUBMB LIFE  (2007)

Tempol diverts peroxynitrite/carbon dioxide reactivity toward albumin and cells from protein-tyrosine nitration to protein-cysteine nitrosation

Augusto, O; Alves, MJM; Fernandes, DC; Medinas, DB

Article ISI
FREE RADICAL BIOLOGY AND MEDICINE  (2005)

CaseReport (1)

Desafíos en el diagnóstico de enfermedad de Creutzfeldt-Jakob: Caso clínico

Marcos Ramírez; Andrés Gallardo; Aarón Vidal; Sebastián Cornejo; Darío Ramírez; Danilo Medinas; Gonzalo Bustamante; Renzo Pasquali; Claudio Hetz

CaseReport ISI SCIELO SCOPUS
REVISTA MEDICA DE CHILE  (2016)

EditorialMaterial (1)

ER stress links aging to sporadic ALS

Medinas DB; Cabral-Miranda, F; Hetz, C

EditorialMaterial ISI SCOPUS
AGING-US  (2019)

Note (1)

ERp57 as a novel cellular factor controlling prion protein biosynthesis: Therapeutic potential of protein disulfide isomerases

Sepúlveda M.; Rozas P.; hetz C.; Medinas D.B.

Note ISI SCOPUS
PRION  (2016)

Proyecto (5)

DEFINING THE CONTRIBUTION OF ERP57 MUTANTS TO THE PATHOGENESIS OF AMYOTROPHIC LATERAL SCLEROSIS

FONDECYT (11150579)

Proyecto
2015-2018
Inv. Responsable

DEFINING MECHANISMS AND THE ROLE OF AGGREGATION OF WILD-TYPE SUPEROXIDE DISMUTASE 1 (SOD1) IN AMYOTROPHIC LATERAL SCLEROSIS

FONDECYT (3130351)

Proyecto
2012-2015
Inv. Responsable

Therapeutic Use of delta Protein Kinase C in Parkinson's Disease

Stanford University Dean's Office (Aaron Fund)

Proyecto
2011-2011
Postdoctorado

Aldehyde Dehydrogenase Activators as Novel Drugs For the Treatment of Parkinson's Disease

Michael J Fox Foundation for Parkinson's Research (RRIA 2009)

Proyecto
2009-2010
Postdoctorado

Papéis de Oxidantes e Radicais Livres em Esclerose Lateral Amiotrófica (ELA). Implicações em Outros Processos Neurodegenerativos.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (04/03781-4)

Proyecto
2005-2009
Tesista

Review (2)

Proteostasis disturbance in amyotrophic lateral sclerosis

Medinas, DB; Valenzuela, V; hetz, C

Review ISI SCOPUS
Human Molecular Genetics  (2017)

The ER proteostasis network in ALS: Determining the differential motoneuron vulnerability

Rozas P.; Bargsted L.; Martínez F.; Hetz C.; Medinas D.B.

Review ISI SCOPUS
NEUROSCIENCE LETTERS  (2017)

17
Danilo Bilches

Staff Scientist

Instituto de Neurociencia Biomedica

Santiago, Chile

6
Claudio Hetz

Deputy Director

INSTITUTO DE CIENCIAS BIOMÉDICAS, FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

SANTIAGO, Chile

5
Pablo Rozas

Investigador

SymbiOx

Santiago, Chile

4
Felix Diaz

3
Ute Wohlbier

Associate Professor

Center for Integrative Biology

Universidad Mayor de Chile

Santiago, Chile

2
Catherine Andreu

Estudiante de Doctorado

Escuela de Psicología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Maria Matus

Principal Investigator

Fundacion Ciencia & Vida

Santiago, Chile

2
JUAN PABLO HENRIQUEZ

Associate Professor

Cell Biology

UNIVERSIDAD DE CONCEPCIÓN, FACULTAD DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGÍA CELULAR

Concepción, Chile

2
Felipe Court

Full Professor and Director

Center for Integrative Biology

Universidad Mayor

Huechuraba, Chile

2
sandra espinoza

asistente de investigacion

Laboratorio Neuroinmunologia

Fundacion Ciencias para la Vida/Universidad San Sebastian

Santiago, Chile

1
Patricio Manque

VICE PRESIDENT FOR RESEARCH AND FULL PROFESSOR

UNIVERSIDAD MAYOR

Santiago, Chile

1
Jorge Ojeda

Académico investigador

Ciencias Básicas y Morfología

Universidad Católica de la Santisima Concepción

Concepción, Chile

1
Viviana Pérez

Profesor Asistente

Fisiología

Universidad de Concepción

Concepcion, Chile

1
Ariel Castro

Full Professor

Department of Biochemistry and Molecular Biology

UNIVERSIDAD DE CONCEPCION

concepciòn, Chile

1
René Vidal

Profesor Asistente

Universidad Mayor

Santiago, Chile

1
Agustin Martinez

Profesor Titular

Instituto de Neurociencia

UNIVERSIDAD DE VALPARAÍSO, CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAÍSO

Valparaíso, Chile

1
Diego Rojas

Assistant Professor

Center for Integrative Biology

Universidad Mayor

Santiago, Chile

1
Melissa Calegaro

Principal Investigator, head lab

Research Vice Presidency

Universidad Mayor

Santiago, Chile

1
Karina Palma

Postdoctorado

Instituto de Neurociencia Biomédica BNI

Universidad de Chile

Santiago, Chile

1
Jose Matamala

Assistant Professor of Neurology

Department of Neurological Science

University of Chile

Santiago, Chile

1
Vicente Valenzuela

Investigador postdoctoral

Instituto de Neurociencia Biomédica

Santiago, Chile

1
Adrian Palacios

Profesor

Instituto de Neurosciencia

UNIVERSIDAD DE VALPARAISO

Valparaiso, Chile

1
Fernando Bustos

Profesor Asistente

Facultad de Medicina

Universidad Andres Bello

Santiago, Chile

1
Víctor Cornejo

Profesor Asistente

Facultad de Ciencias Biológicas

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Cristina Pinto

Doctor en ciencias biologicas

Centro de Biotecnología

Universidad de Concepción

Concepción, Chile

1
Alvaro Ardiles

Full Professor

Medicine

Universidad de Valparaíso

Valparaiso, Chile

1
Valentina Castillo

Investigadora Postdoctoral

Instituto de Ingeniería Biológica y Médica

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Alfredo Sagredo

Investigador Postdoctoral

ICBM

Universidad de Chile

Santiago, Chile

1
Andrés Gallardo

1
Claudia Duran-Aniotz

Profesor Asociado

Escuela de Psicología

Universidad Adolfo Ibánez

Santiago, Chile

1
Francisco Fuentes

Profesor Asistente

Departamento de Microbiología

Universidad de Concepción

Concepción, Chile

1
Brigitte van Zundert

Full Professor

UNIVERSIDAD ANDRÉS BELLO, CENTRO DE INVESTIGACIONES BIOMÉDICAS

Santiago, Chile

1
Pablo Henny

Profesor Asociado

Anatomía Normal

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Ricardo Armisen

Associate Professor

Centro de Genética y Genómica

Universidad del Desarrollo

Santiago, Chile

1
Alicia Colombo

Profesora Asociada/Director Científico Biobanco de Tejidos y Fluidos de la Universidad de Chile

Departamento de Anatomía Patológica

Universidad de Chile

Santiago, Chile

1
Nelson Osses

Associated Professor

Institute of Chemistry

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

1
Fabiola Osorio

Associate Professor

Programa Disciplinario de Inmunologia

Universidad de Chile

Santiago, Chile