Local and systemic immune response to bacterial biofilms Innovative therapies for inflammatory diseases

Undergraduate thesis supervision
1. "Role of the O antigen of the lipopolysaccharide of Porphyromonas gingivalis in the profile of differentiation of macrophages". Student: Alfredo Ignacio Castro Martínez. 2019. Financing: FONDECYT 1160741.
2. Cardiovascular risk associated with systemic inflammation in patients with asymptomatic apical periodontitis. Student: Francisca Quinlan Binelli, year 2019. Financing: FONDECYT 1160741.
3. Levels of expression of toll-4 receptor (TLR-4), interleukin (IL) -10 and IL-12 in peripheral blood mononuclear cells of patients with asymptomatic apical periodontitis. Student: Lynn Barstow Melgarejo, year 2019. Financing: FONDECYT 1160741.
4. Role of the O antigen of the lipopolysaccharide of Porphyromonas gingivalis in the profile of differentiation of macrophages. Student: Alfredo Castro Martínez, year 2019. Financing: FONDECYT 1160741.
5. Immunolocalization of the signaling complex of interleukin-6 in periapical lesions of endodontic origin. Student: Catalina Schweitzer Gatica, year 2019. Financing: FONDECYT 1160741.
6. Prevalence of teeth treated endodontically in a Chilean subpopulation. Student: Macarena Becker Pinto, year 2018. Financing: FONDECYT 1160741. 2018.
7. "Humoral immune response and endotoxemia in endodontic infection with Porphyromonas endodontalis and Porphyromonas gingivalis in Asymptomatic Apical Periodontitis. "Dept. of Pathology and Oral Medicine. University of Chile. Student: student: Valeska Fariña Espinosa. Year 2015. Financing: FONDECYT 1120138.
8. "Morphological and Molecular Characterization of Mandibular Temporomal Ankylosis". Dept. of Pathology and Oral Medicine. University of Chile. Student: student Rubén Soto Galaz. Year 2015. Financing: FIOUCH13-004.
9. "Levels of Tartrate-Resistant Acid Phosphatase (TRAP), Ligand Activating Receptor for the Nuclear Factor NFkB (RANKL) and Osteoprotegerin (OPG) in Symptomatic and Asymptomatic Periapical Lesions of Endodontic Origin". Dept. of Pathology and Oral Medicine. University of Chile. Student: Macarena Salinas Muñoz. Year 2015. Financing: FONDECYT 1120138
10. Myeloperoxidase (MPO) levels in Periapical Lesions of Endodontic Origin. Dept. of Pathology and Oral Medicine. University of Chile. Student: Marcela Bolbarán Gómez. Main tutor Year 2014. FONDECYT financing 1120138.
11. C-Reactive Protein in Apical Lesions of Endodontic Origin. Department of Conservative Dentistry and Pathology and Oral Medicine. University of Chile. María José Bordagaray Principal Tutor. Year 2013. Financing FONDECYT 1120138.
12. "Association between Asymptomatic Apical Periodontitis and Modifiable Risk Factors of Cardiovascular Diseases" Germán Contreras Silva Associate Tutor. Financing FONDECYT 1120138. Year 2013.
13. "Levels of Myeloperoxidase (MPO) in Periapical Teeth Exudate with Asymptomatic Apical Periodontitis (PAA) Measured Pre and Post-Endodontic Medications with Calcium Hydroxide". Josefa Belén Castro Salas. Associate tutor. Year 2013. Financing FONDECYT 1120138.
14. Tissue Localization of Matrix Metalloproteinase -12 in Periapical Lesions Consecutive to Septic Pulp Necrosis and Healthy Periapical Tissue, Student: Pilar Jerez. School of Dentistry University of Chile. Associate tutor. Year 2012. Financing FONDECYT 1090461.
15. Levels of Myeloperoxidase (MPO) in Crevicular Gingival Fluid (FGC) of Teeth with Asymptomatic Apical Periodontitis (PAa). Student: Valentina Aranda González. Responsible tutor Year 2012. Financing FONDECYT 1090461.
16. Thesis Levels of Crosslinked Piridinoline of Type I Colloid Tipelactopeptide (ICTP) in Crevicular Gingival Fluid (FGC) of Teeth with Asymptomatic Apical Periodontitis (PAa). Student: Gianfranco Arce Salvi. Year 2012. Financing FONDECYT 1090461.
17. Levels of Vascular Endothelial Growth Factor (VEGF-A) in Periapical Lesions Consecutive Pulp Necrosis and Periodontal Ligament. Student: Magdalena Infante Figueroa. Year 2011. Financing FONDECYT 1090461.
18. Levels of Vascular Endothelial Growth Factor (VEGF) and Interleukin-1B in Gingival Crevicular Fluid (FCG) of Teeth with Asymptomatic Apical Periodontitis (PAa) Pre and Post Endodontic Treatment (2011). Student: Gustavo Pérez Serrano. Year 2011. Financing FONDECYT 1090461.
19. Levels of ICTP in Asymptomatic Apical Periodontitis and its Association with Periradicular Tissues. Student: Pamela Giralt Year 2011. Financing FONDECYT 1090461.
20. Levels of Cathepsin K in Crevicular Teeth Gingival Fluid with Asymptomatic Apical Periodontitis. Student: Loreto Espinoza. Year 2010. Financing FONDECYT 1090461.
21. Expression of Extracellular Matrix Metalloproteinase-13 (MMP-13) in Teeth with Asymptomatic Apical Periodontitis. Student: Andrea González. Year 2010. Financing FONDECYT 1090461.

Postgraduate Thesis (Master and Doctorate):
1. Polarization of macrophages in clinically symptomatic forms of apical periodontitis: Role of Lipopolysaccharide of Porphyromonas species on the expression of Toll-like receptors and their epigenetic regulation. Doctorate Program in Dental Sciences, University of Chile. Responsible tutor Student: Pablo Veloso Matta, year 2018. Financing FONDECYT 1160741. Status: Qualification exam approved.
2. Systemic inflammation and endodontic infection by Porphyromonas endodontalis in patients with asymptomatic apical periodontitis. Master's Program in Dental Sciences mention Periodontology, University of Chile. Responsible tutor Student: Patricia Huamán Chipana, year 2017. Financing FONDECYT 1120138.
3. Effects of the stimulation of human periodontal ligament fibroblasts with IL-6 on the synthesis of C-reactive protein (CRP) and proinflammatory cytokines. Master's Program in Dental Sciences mention Periodontology, University of Chile. Responsible tutor Student: Arnoldo Hernández Caldera, 2015. Funding FONDECYT 1120138.
4. Effect of stimulation with the different serotypes of Porphyromonas gingivalis and Aggregatibacter actinomycetencomitans on the activity of TCD4 + lymphocytes and bone resorption in vitro. Master's Program in Dental Sciences mention Periodontology, University of Chile. Associate tutor. Student: Student Melgar Samanta Rodríguez. Year 2014. FONDECYT 11100298.
5. Markers of bone metabolism in gingival crevicular fluid as a potential diagnostic tool for chronic inflammatory diseases of the marginal and apical periodontium. Master's Program in Odontological Sciences mention Oral Pathology. Principal tutor Student Mauricio Baeza Paredes 2014. Financing: FONDECYT 1120138.
6. Systemic inflammation in serum of patients with asymptomatic apical periodontitis. Master's Program in Odontological Sciences mention Oral Pathology. Principal tutor Student Fabián Obregón Miano year 2014. Financing: FONDECYT 1120138.
7. Effect of extracellular matrix metalloproteinases on TNF levels ?; IL-1 ?; SDF-1 / CXCL12 and VEGF in human periodontal ligament fibroblasts stimulated with hydrogen peroxide. Research work to qualify for the Master's degree in Dental Sciences. Program: Master in Cs. Dentistry mention Periodontology, University of Chile, Student: Franco Cavalla. 2013. Main tutor. Financing: FONDECYT 1090461.
8. Effect of H2O2 on the activity of alkaline phosphatase, cytokine and extracellular matrix metalloproteinase levels with osteolytic activity in human periodontal ligament fibroblasts, Magister in Cs. Dentistry mention Periodontology, University of Chile, Faculty of Dentistry. Student: Constanza Osorio 2012. Funding: FONDECYT 1090461.
9. Levels and Activity of Extracellular Matrix Metalloproteinases -2, -9, -13 and? 2-macroglobulin in teeth with Chronic Apical Periodontitis. Magister program in Cs. Dentistry mention Periodontology, University of Chile, Student (a): Verónica Mundi, 2012. Financing: FONDECYT 1090461.

Invited Conferences - National:

1. Monocyte-macrophage mechanisms in the periodontal-systemic cross-talking. Symposium on periodontal and regenerative medicine, Chilean Society of Periodontology and University of Los Andes, December 1-2, 2023, University of Los Andes, Santiago, Chile.
2. Maladaptive responses of monocytes/macrophages in periodontitis and associated non-communicable diseases. Seminar of the Chilean Society of Immunology (ASOCHIN-International Association of Dental Research IADR, Chile Division), September 12, 2023, Zoom platform.
3. Programming of monocytes/macrophages in inflammatory periodontal destruction and systemic risk. Inaugural Conference of the IV Congress of the Institute of Odontological Sciences, Faculty of Dentistry, University of Chile (ICOD), Santiago, August 1, 2024.
4. Apical periodontitis, a systemic disease. First Clinical Research Day of the International Association for Dental Research (IADR) “Meeting of Dentistry and Science in the South,” April 14-15, 2023, Valdivia, Chile.
5. Healthy periodontium for a healthy heart: Fundamentals and clinical considerations in periodontitis and endodontic infections. Research Days in Dentistry, UFRO Chile, November 25, 2022, Temuco, Chile.
6. Macrophage polarization profiles and their epigenetic regulation: Role of lipopolysaccharide from Porphyromonas species. II Annual Congress ICOD, “Frontier Sciences in Dentistry,” December 18, 2020, online format.
7. Macrophage differentiation profiles and their epigenetic regulation in periapical lesions of endodontic origin: Possible implications for systemic risk. XXXI Annual Meeting IADR Chile Division, July 31, 2020, online format.
8. Mechanisms of dialogue between periapical response and non-communicable diseases. First Symposium on Oral Pathology and Research, July 24, 2020, online format, Universidad San Sebastián.
9. Gender challenges in endodontic medicine research. XXXI Annual Meeting IADR Chile Division, August 30, 2019, Santiago, Chile.
10. Endodontic infection: An emerging cardiovascular risk factor? XXX Annual Meeting of the IADR Chile Division, September 7, 2018, Santiago, Chile.
11. Endodontic Medicine: Emerging Evidence of Systemic Risk and Dental Management Considerations. “VII version of the Evidence-Based Dentistry Symposium, SOBE” National Association of Dental Students, ANACEO Chile, June 1, 2018, Austral University, Valdivia.
12. Immunoinflammatory response as a link between endodontic oral infection and cardiovascular risk. Cycle of Seminars Institute of Biomedical Sciences, Autonomous University of Chile, November 23, 2017, Santiago, Chile.
13. Impact on dental practice of current research in Dental Sciences. November 3, 2017, Autonomous University, Santiago, Chile.
14. Inflammatory Response in Apical Periodontitis and Systemic Risk. Society for Oral Maxillofacial Pathology, October 11, 2017, College of Dental Surgeons, Santiago, Chile.
15. Formation of research teams in evaluating periapical inflammation as a systemic risk factor. XXII CONADEO, Concepción, September 11, 2017.
16. Basic tools in dental research. Seminar on Research in Dentistry, Autonomous University, Temuco, Chile.
17. Periodontal biomarkers in oral fluids: From research to dental practice and Expert Panel. First Conference on Innovation and Technology Transfer in Dentistry, Faculty of La Frontera, Temuco, May 19, 2017.
18. Clinical application of biomarkers in oral fluids for the diagnosis and monitoring of periodontal diseases and their systemic complications. Translational research session “From Science to Clinic” XXI National Congress of Dental Students CONADEO, August 6, 2016, Pontificia Universidad Católica de Chile, Santiago, Chile.
19. Local and Systemic Inflammation in Asymptomatic Apical Periodontitis and its response to Endodontic treatment. Endodontic Society of Chile, November 18, 2015, San Sebastián University, Santiago, Chile.
20. Relevance of the Diagnosis and Treatment of Periapical Pathology in the context of General Medicine. Universidad del Desarrollo School of Medicine, August 21, 2015, Santiago, Chile.
21. Morphological Aspects and Biology of TMJ Ankylosis. Society of Oral and Maxillofacial Surgery and Traumatology of Chile, August 13, 2015, Clínica Alemana, Santiago, Chile.
22. Asymptomatic Apical Periodontitis: Local Inflammation or Systemic Response? Scientific Meeting, Society of Endodontics of Chile, October 14, 2014, Santiago, Chile.
23. Crosstalk Between Local Host Response to Endodontic Infection and Low-Grade Systemic Inflammation in Asymptomatic Chronic Periodontitis: Prospective Study Evaluating the Effect of Conservative Endodontic Treatment. Second Research Day in Dentistry, Faculty of Dentistry, University of Chile, January 17, 2013, Santiago, Chile.
24. Host Response and Development of Biomarkers in Chronic Periodontitis. Scientific Meeting, Society of Periodontology of Chile, October 22, 2011, Santiago, Chile.
25. Host-Derived Destruction Mediators and Their Role as Potential Biomarkers in Periodontal Pathology. First International Symposium on Odonto-Bone Research Mechanisms of Regeneration, Remodeling, and Odonto-Bone Pathologies, Faculty of Dentistry, University of Chile, January 19, 2011, Santiago, Chile.
26. Periapical Lesions: New Concepts and Future Perspectives. Fifth National Conference on Oral and Maxillofacial Pathology, Faculty of Dentistry, University of Chile, January 21, 2010, Santiago, Chile.
27. Periodontal and Endodontic Pathology: New Diagnostic and Therapeutic Tools. Symposium on Bone Biology, “Cellular and Molecular Mechanisms in Bone Pathology,” XXII Annual Meeting IADR Chile Division, October 23, 2009, Universidad Andrés Bello, Santiago, Chile.
28. Inflammatory Mediators and Progression of Chronic Apical Periodontitis: A Potential Contribution to Clinical Practice? Society of Oral Pathology of Chile, XXII Annual Meeting IADR Chile Division, October 23, 2009, Universidad Andrés Bello, Santiago, Chile.
29. FGC: A New Tool for Studying Chronic Apical Periodontitis? Scientific Meeting, Society of Endodontics of Chile, October 21, 2009, Santiago, Chile.
30. Molecular Study of Endodontic Pathology. Scientific Meeting, Society of Endodontics of Chile, November 19, 2008, Santiago, Chile.
31. Periapical Lesions: New Methodologies for Diagnosis and Clinical Monitoring. Department of Pathology-Institute of Oral Pathology Reference, IREPO IV National Conference on Oral-Maxillofacial Pathology, January 2008, Santiago, Chile.
32. MMPs and Tissue Destruction Profiles Associated with Chronic Periodontitis. XX Annual Meeting of the International Association for Dental Research (IADR), December 10, 2007, Faculty of Dentistry, University of Chile, Santiago, Chile.

Invited Conferences - International :
1. Challenges of Dental Design in Evidence-Based Dentistry. Iberopan American Federation of Periodontology, May 8, 2023. Online modality.
2. "Oral infections and systemic diseases". Oral Gut Symposium “Mucosal immunity at the Oral-Gut axis in health or disease” Fundación Ciencia & Vida, January 14th, 2020.
3. Periapical lesion: The systemic cost of containing the endodontic infection. First international meeting of IADR, Caribbean section. October 4, 2019, Santo Domingo, Dominican Republic.
4. Preparation and publication of a scientific article: Key to success. First international meeting of IADR, Caribbean section. October 4, 2019, Santo Domingo, Dominican Republic.
5. Scientific methodology applied to dental clinical research. XIV International Conference on Science and Technology, National University of North east. September 27 Corrientes Argentina.
6. Crosstalk between local and systemic host’s inflammatory response in endodontic infection. XXIV Latin American Congress of Microbiology, November 16, 2018, Santiago Chile.
7. Apical lesions of endodontic origin: A reservoir of systemic inflammation of cardiovascular risk. XII International Congress of Dentistry National University Federico Villarreal. October 24, 2018, Lima Peru.
8. "Biological bases of endodontic medicine: Emerging evidence of cardiovascular risk" VII Congress of the Latin American Region IADR, September 1, 2018, Montevideo, Uruguay.
9. “Regulation of the periodontal inflammatory response by interleukin (IL) -6” Molecular Pathology Symposium, XIV Meeting of the Ibero-American Academy of Pathology and Oral Medicine (AIPMB), August 29, 2016 Lima Peru.
10. Differential Diagnosis of Apical Lesions of Endodontic origin, Postgraduate and Research Unit, Faculty of Dentistry, Autonomous University of Yucatán November 12, 2015 Faculty of Dentistry, Autonomous University of Yucatán, Mérida, Mexico.
11. The Inflammatory Response in Apical Periodontitis as a Cardiovascular Risk Factor. Postgraduate and Research Unit, Faculty of Dentistry, Autonomous University of Yucatán November 11, 2015 Faculty of Dentistry, Autonomous University of Yucatán, Mérida, Mexico.
12. Distribution of Gingival Lesions Not Induced by Plaque in Chilean Patients. Faculty of Dentistry, University of Barcelona, Master's program for Diagnostic Updating and Medical Therapeutics in Oral Medicine. June 4, 2015. Faculty of Dentistry, University of Barcelona, Barcelona, Spain.
13. Regulation of the Periodontal Inflammatory Response by Interleukin (IL)-6. Symposium on Molecular Pathology, XIV Meeting of the Ibero-American Academy of Pathology and Oral Medicine (AIPMB), Lima, Peru, August 29, 2016.
14. The Inflammatory Response in Apical Periodontitis as a Cardiovascular Risk Factor. Postgraduate and Research Unit, Faculty of Dentistry, Autonomous University of Yucatán. Faculty of Dentistry, Autonomous University of Yucatán, Mérida, Mexico. November 11, 2015.
15. Differential Diagnosis of Apical Lesions of Endodontic Origin. Postgraduate and Research Unit, Faculty of Dentistry, Autonomous University of Yucatán. Faculty of Dentistry, Autonomous University of Yucatán, Mérida, Mexico. November 12, 2015.
16. Distribution of Non-Plaque-Induced Gingival Lesions in Chilean Patients. Faculty of Dentistry, University of Barcelona, Master’s Program in Diagnostic and Therapeutic Updates in Oral Medicine. Faculty of Dentistry, University of Barcelona, Barcelona, Spain. June 4, 2015.
17. Dialogue between the Local and Systemic Inflammatory Response in Apical Periodontitis. XII Meeting of the Ibero-American Academy of Oral Pathology and Medicine. 1 h Santiago Chile. November 13, 2014.
18. Crosstalk between inflammatory mediators and matrix metalloproteinases in apical lesions of endodontic origin. PhD Program in Dental Sciences, Institute of Dentistry University of Helsinki, 1h. Biomedicum Helsinki, University of Helsinki, Helsinki Finland. September 3, 2014.
19. Non-plaque induced gingival lesions: Pitfalls in the AAP Classification of Periodontal Diseases. Dental Education Program (Undergraduate), Faculty of Dentistry, University of Helsinki, 1h Helsinki Finland. September 9, 2014-.
20. Bases of the Methodological Design Applied to Clinical Research in Dentistry. Pontificia Universidad Católica Madre y Maestra Santiago and Santo Tomás de Aquino Campus, 8 pm, Dominican Republic, Santiago de los Caballeros and Santo Domingo. March 24-27, 2014.
21. Biomarkers in FCG for the diagnosis and monitoring of chronic periodontitis. Pontificia Universidad Católica Madre y Maestra Santiago Campus, Santiago de los Caballeros, Dominican Republic. March 25, 2014.
22. Biomarkers in FCG for the diagnosis and monitoring of chronic periodontitis. 1 hour. Pontificia Universidad Católica Madre y Maestra Santo Tomás de Aquino Campus, Santo Domingo, Dominican Republic. March 27, 2014.
23. Panelist Round Table, International Periodontology Course, Society of Periodontology of Chile, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile. March 16, 2013.
23. Proteolytic pathways involving MMP-13 and -8 in progressive chronic periodontitis Biomedicum University of Helsinki, Institute of Dentistry, Helsinki, Finlandia. 13 de mayo de 2009.