Man

Guillermo Antonio Díaz Araya

Académico

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

Líneas de Investigación


Farmacología molecular cardiovascular; fibrosis cardiaca; patologias cardiovasculares; Inflamación; Estrés oxidativo

Educación

  •  Ciencias Biológicas, Universidad Autónoma de Madrid. España, 1992

Experiencia Académica

  •   Académico Full Time

    UNIVERSIDAD DE CHILE

    Ciencias Químicas y Farmacéuticas

    Santiago de Chile, Chile

    1993 - A la fecha

Experiencia Profesional

  •   Académico Full Time

    Universidad de Chile

    Santiago, Chile

    1994 - A la fecha

Formación de Capital Humano


PhD thesis finished

1. Claudio Humeres Martínez: "Differential role of TLR4 on the adhesion and activation of m1 / m2 macrophages in fibroblasts and cardiac myofibroblasts". Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Completion date November 2016.

2. Pía Boza Fuentes: "New role of Angiotensin II: activates the inflammasome in fibroblasts and cardiac myofibroblasts". Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Completion date November 2016.

3. Samir Bolivar González. Interferon Beta decreases the expression of pro-inflammatory cytokines and the fibrotic response in the cardiac fibroblast of adult rats. Doctoral Thesis in Pharmaceutical Sciences. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date ended July 2018.

4. Glauben Landskron: "Role of extracellular ATP in the release of IL33 by cancer-associated fibroblasts and the effect of this cytokine on the epithelial-mesenchymal transition of a colorectal adenocarcinoma cell line", PhD Thesis in Biomedical Sciences. Faculty of Medicine, University of Chile. Completation date December 2018.

5. Claudia Muñoz Rodríguez: "Effect of LPS on the expression of receptors B1 and B2 of kinins in fibroblasts and cardiac myofibroblasts". Doctoral Thesis in Pharmaceutical Sciences. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. ECompletatio date January 2019.

6. Viviana Pardo Jiménez: "Synthesis of new coumarin derivatives and evaluation of their inhibitory activity of histone deacetylase in cardiac fibroblasts". Doctoral Thesis in Pharmaceutical Sciences. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. May 2019.

PhD Thesis in course

7. Pablo Parra: "Optimization of the treatment with Vitamin C, N-acetyl cysteine ??and Desferoxamine in the prevention of death by ischemia reperfusion in an in vivo and in vitro model in cardiac fibroblasts of adult rats. Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date September 2016.

8. Paula Valenzuela: Evaluación comparativa de la actividad cicatrizante cutánea de extractos concentrados en fenoles y en triterpenoides obtenidos de hojas de distintos genotipos de Ugni molinae Turcz. Doctorate Thesis in Pharmaceutical Sciences. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date September 2016.

9. Aimée Salas: Effect of Resolvin D1 on the proinflammatory effect triggered by Ang II in cardiac fibroblasts. Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date September 2017.

10. Francisco Olivares Silva: Resolvina D1 previene la hipertensión cardiaca inducida por Ang II. Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date April 2018.

12. Ileana Carrillo. Resolvina D1 gatillada por aspirina (AT-RvD1) limita el progreso de la Cardiomiopatía Chagásica Crónica, disminuyendo la expresión de TNF? y la apoptosis de células musculares cardíacas. Doctorate Thesis in Pharmacology. Faculty of Chemical and Pharmaceutical Sciences, University of Chile. Start date June 2019.

Undergraduate Thesis

Finished
2019
- Resolvina-D1 a través del receptor ALX/FPR2 aumenta la síntesis de colágeno en miofibroblastos cardiacos de rata adulta. Memory to obtain the title of Chemist-Pharmacist by Esteban Castro Carrasco. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

- Efecto de Angiotensina II sobre los niveles de mRNA de citoquinas y moléculas de adhesión en fibroblastos cardiacos. Memory to obtain the title of Chemist-Pharmacist by Francisca Bruggendieck. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

2018
- La Resolvina D1 previene el aumento de ICAM y VCAM inducido por heparan sulfato en fibroblastos cardiacos. Memory to obtain the title of Chemist-Pharmacist by Víctor Aaron Velarde Lipín. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

-Vías de señalización intracelulares activadas por Resolvina D1 y E1 en fibroblastos cardiacos. Memory to obtain the title of Chemist-Pharmacist by Jossete Cayupi Vivanco. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

- Resolvina E1 previene el aumento de ICAM-1 inducido por Ang II en fibroblastos cardiacos. Memory to obtain the title of Chemist-Pharmacist by Felipe Ruz Cortés. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

 2017
• Effect of Resolvin D1 on the viability and expression of ICAM-1 in cardiac fibroblasts. Memory to obtain the title of Chemist-Pharmacist by Ninoska Ramírez. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Effect of Resolvin E1 on the expression of VCAM-1 in cardiac fibroblasts. Memory to obtain the title of Chemist-Pharmacist by Natalia Muñoz. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Transduction pathways activated by RvD1 and cardiac fibroblasts. Memory to obtain the title of Chemist-Pharmacist by Pablo Sepúlveda. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

2016
• Effect of Heparan sulfate on the expression of cytokines and chemokines in cardiac fibroblasts: effects on monocyte chemoattraction. Report to obtain the title of Chemist-Pharmacist by Francisco Olivares Silva. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Expression of E-selectin in cardiac fibroblasts: effects on neutrophil binding and activity of MMPs, and change in phenotype to myofibroblast. Memory to obtain the title of Chemist-Pharmacist by José Miguel Osorio. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Expression of IL-8 in cardiac fibroblasts: effects on the chemoattraction of neutrophils. Renatto Anfossi. Report to obtain the degree of Chemist-Pharmacist by Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Expression of VCAM, ICAM by Bradykinins in cardiac fibroblasts: effects on the adhesion of neutrophils. Report to obtain the title of Chemist-Pharmacist by Samuel Fernández Sandoval. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.
    
2015
• Transduction pathways activated by TLR4 that control the expression of VCAM and ICAM. Memory to obtain the title of Chemist-Pharmacist by Elias Inostroza. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Comparative study of the expression and activity of the NLRP3 inflammasome in fibroblasts and cardiac myofibroblasts. Memory to obtain the title of Chemist-Pharmacist by Felipe Tapia. Faculty of Chemical and Pharmaceutical Sciences, University of Chile

• Heparan Sulfate, through TLR4, triggers both the increase in IL-1?, ICAM and VCAM, and the decrease in ?-SMA. Memory to obtain the title of Chemist-Pharmacist by Rodolfo Landaeta. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Participation of TLR4 receptors in the protection against cardiac fibroblast death induced by hypoxia / reoxygenation. Memory to obtain the title of Chemist-Pharmacist by Cristian Queirolo. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.

• Participation of TLR4 in the differentiation of fibroblast to myofibroblast. Memory to obtain the title of Chemist-Pharmacist by Roxana Santana. Faculty of Chemical and Pharmaceutical Sciences, University of Chile.



 

Article (18)

Synthesis and Biological Evaluation of Novel Thiazolyl-Coumarin Derivatives as Potent Histone Deacetylase Inhibitors with Antifibrotic Activity.
FoxO1 mediates TGF-beta1-dependent cardiac myofibroblast differentiation.
Expression and function of toll-like receptor 4 and inflammasomes in cardiac fibroblasts and myofibroblasts: IL-1β synthesis, secretion, and degradation.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Cardiac fibroblasts as sentinel cells in cardiac tissue: Receptors, signaling pathways and cellular functions.
H2 O2 activates matrix metalloproteinases through the nuclear factor kappa B pathway and Ca(2+) signals in human periodontal fibroblasts.
4-Phenylbutyric acid prevent cytotoxicity induced by thapsigargin in rat cardiac fibroblast.
A novel dihydropyridine with 3-aryl meta-hydroxyl substitution blocks L-type calcium channels in rat cardiomyocytes.
Angiotensin-(1-9) reverses experimental hypertension and cardiovascular damage by inhibition of the angiotensin converting enzyme/Ang II axis.
Changes in iron transporter divalent metal transporter 1 in proximal jejunum after gastric bypass.
EPAC expression and function in cardiac fibroblasts and myofibroblasts.
Regulation of cardiac autophagy by insulin-like growth factor 1.
TGF-β1 prevents simulated ischemia/reperfusion-induced cardiac fibroblast apoptosis by activation of both canonical and non-canonical signaling pathways.
Attenuation of endoplasmic reticulum stress using the chemical chaperone 4-phenylbutyric acid prevents cardiac fibrosis induced by isoproterenol.
Dexmedetomidine preconditioning activates pro-survival kinases and attenuates regional ischemia/reperfusion injury in rat heart.
Simvastatin disrupts cytoskeleton and decreases cardiac fibroblast adhesion, migration and viability.
Beta(2)-adrenergic receptor regulates cardiac fibroblast autophagy and collagen degradation.
Simvastatin induces apoptosis by a Rho-dependent mechanism in cultured cardiac fibroblasts and myofibroblasts.

Proyecto (18)

Proinflammaroty mechanisms in cardiovascular diseases: targets for pharmacological interference"
Resolvin D1 and E1 novel pharmacological targets for preventing hypertension-dependent cardiac fibrosis: a focus on the proinflammatory role of cardiac fibroblasts
Effect of Exercise Training in Inflammation, Oxidative Stress, Pro-fibrotic miRNA Levels and Left Ventricular Response to Exertion in Patients with Heart Failure with Preserved Ejection Fraction Hugo Verdejo / Guillermo Díaz Araya
Centro Avanzado para las enfermedades crónicas ACCDiS
OX-LDL/LOX-1/NADPH OXIDASE SIGNALING PATHWAY IN CARDIAC MYOFIBROBLAST=> NOVEL ROLE IN THE REGULATION OF AUTOPHAGY,SURVIVAL AND PROFIBROTIC RESPONSE
Neuroendocrine regulation of the rat ovary: Prenatal exposure to sympathetic stress and its transgenerational effects on the reproductive function of the progeny
ROLE OF TLR4 AND INFLAMMASOME IN THE DIFFERENTIATION AND ACQUISITION OF A PROINFLAMMATORY PHENOTYPE OF ADULT RAT CARDIAC MYOFIBROBLAST
KININ AND BETA2-ADRENERGIC RECEPTORS IN CARDIAC MYOFIBROBLASTS AND THEIR RELATIONSHIPS WITH CARDIAC FIBROSIS
Neonatal exposure to estradiol and stress in rats activates neural genes and programs sympathetic nerve activity to determine changes in reproductive function and polycystic ovary development.
RESISTENCIA A INSULINA EN LA DIABETES TIPO II=> ESTUDIO DE NUEVOS MECANISMOS FISIOPATOLOGICOS ASOCIADOS AL DETERIORO DE LA FUNCION CARDIACA
ANGIOTENSINA II Y SUS RECEPTORES SUBTIPOS AT1 Y AT2 EN LA REGULACION DE LA FUNCION Y SOBREVIDA DEL FIBROBLASTO Y MIOFIBROBLASTO CARDIACO.
ESTRES OXIDATIVO, INFLAMACION Y DISFUNCION ENDOTELIAL EN LA INSUFICIENCIA CARDIACA CRONICA=>EFECTOS DE LA INHIBICION DE LA XANTINO-OXIDASA Y 3-HIDROXI-3-METILGLUTARIL-CoA REDUCTASA.
ENZIMA CONVERTIDORA DE ANGIOTENSINA I HOMOLOGA (ECA-2) EN LA DISFUNCION VENTRICULAR IZQUIERDA EXPERIMENTAL
Centro de estudios moleculares de la célula FONDAP CEMC
FIBROSIS MIOCARDICA INDUCIDA POR CATECOLAMINAS=> INFLUENCIA DE LAS VARIANTES ALELICAS DEL GEN DE LA ENZIMA CONVERTIDORA DE ANGIOTENSINA-I (ECA) EN SU DESARROLLO.
MECANISMOS DE TRANSDUCCION DE SENALES DEL IGF-1=>REGULACION DIFERENCIAL DE FACTORES TRANSCRIPCIONALES ASOCIADOS A LOS PROCESOS DE HIPERTROFIA Y APOPTOSIS DEL CARDIOMIOCITO
DISOCIACION DE LAS DIFERENTES VIAS TRANSDUCCIONALES DEL FACTOR DE CRECIMIENTO ANALOGO A INSULINA TIPO-1 (IGF-1) EN LOS PROCESOS DE HIPERTROFIA X APOPTOSIS DEL CARBIOMIOCITO
PARTICIPACION DEL IGF-I EN LA GENESIS Y DESARROLLO DE LA HIPERTROFIA CARDIACA PATOLOGICA EXPERIMENTAL=> ESTUDIO Y MODULACION FARMACOLOGICA DE SUS MECANISMOS DE TRANSDUCCION

Article (11)

Role of FoxO3a as a negative regulator of the cardiac myofibroblast conversion induced by TGF-?1
Fibroblast Primary Cilia Are Required for Cardiac Fibrosis
Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal Cancer
The Association of Ascorbic Acid, Deferoxamine and N-Acetylcysteine Improves Cardiac Fibroblast Viability and Cellular Function Associated with Tissue Repair Damaged by Simulated Ischemia/Reperfusion
Expression and function of TLR4-induced B1R bradykinin receptor on cardiac fibroblasts
Heparan sulfate potentiates leukocyte adhesion on cardiac fibroblast by enhancing Vcam-1 and Icam-1 expression
IFN-beta Plays Both Pro- and Anti-inflammatory Roles in the Rat Cardiac Fibroblast Through Differential STAT Protein Activation
Lipopolysaccharide Activates Toll-Like Receptor 4 and Prevents Cardiac Fibroblast-to-Myofibroblast Differentiation
Transforming growth factor-beta and Forkhead box O transcription factors as cardiac fibroblast regulators
Cardiac fibroblast cytokine profiles induced by proinflammatory or profibrotic stimuli promote monocyte recruitment and modulate macrophage M1/M2 balance in vitro
Changes in iron transporter divalent metal transporter 1 in proximal jejunum after gastric bypass

Abstract (3)

Toll-like receptor-4 (TLR4) in the expression of ICAM-1 and VCAM-1 in cardiac fibroblasts and myofibroblasts
Early expression of vascular transforming growth factor beta 1 (TGF beta 1) in DOCA-salt rats
Early decrease in oxidative stress in patients with class IV heart failure after successfull medical therapy

Review (1)

Silencing or not silencing p63 in cardiac fibroblast, risks and benefits
19
Guillermo Díaz

Académico

Química Farmacológica y Toxicológica

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

5
Sergio Lavandero

Director

Advanced Center for Chronic Diseases (ACCDiS)

UNIVERSIDAD DE CHILE, FAC CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

5
Raúl Vivar

Profesor Asistente

Universidad de Chile

Santiago, Chile

5
Claudia Muñoz

Postdoctorado

Universidad de Chile

Santiago, Chile

4
Francisco Olivares

Pharmacology PhD(c)

Department of Pharmaceutical and Toxicological Chemistry

Universidad de Chile

Santiago, Chile

2
Viviana Pardo

Profesional docente

Química Farmacológica y Toxicológica

Universidad de Chile

Santiago, Chile

1
Alfredo Criollo

Assistant professor

Research Institute in Dental Sciences

Facultad de Odontología, Universidad de Chile

Santiago, Chile

1
Paula Valenzuela

Estudiante de Doctorado

Farmacología y Toxicología

Universidad de Chile

Santiago, Chile

1
Constanza Núñez

Investigadora

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

1
Santiago Sepúlveda

Profesional Tecnólogo Médico

Anatomía Patológica

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Pablo Aránguiz

Postdoctorado

Fisiología y Biofísica

Universidad de Chile

Santiago, Chile

1
Mario Chiong

Profesor

Departamento de Bioquímica y Biología Molecular

Universidad de Chile

Santiago, Chile

1
Maria Ocaranza

Asociate Professor

Cardiovascular Diseases Division

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
Carolina Nazzal

jefa

Instituto de Salud Poblacional

Universidad de Chile

Santiago, Chile

1
Juan Roa

Chair

Pathology

Pontificia Universidad Catolica de Chile

santiago, Chile

1
Glauben Landskron

Postdoctoral Researcher Fondecyt 3190931

Academic Direction

Clinica Las Condes

Santiago, Chile