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Veronica Raquel Eisner Sagues

Associate Professor

Pontificia Universidad Católica de Chile

Santiago, Chile

Líneas de Investigación


I focus on Mitochondrial Communication and Function, with special interest in mitochondrial dynamics and intracellular calcium homeostasis. We study mitochondrial diseases and skeletal muscle mitochondria under physio-pathological conditions,

Educación

  •  Food Engineer, UNIVERSIDAD DE CHILE. Chile, 1999
  •  Food sciences and technology, UNIVERSIDAD DE CHILE. Chile, 1997
  •  Biochemistry, UNIVERSIDAD DE CHILE. Chile, 2004

Experiencia Académica

  •   Postdoctoral Fellow Full Time

    UNIVERSIDAD DE CHILE

    Medicine

    Santiago, Chile

    2005 - 2008

  •   Postdoctoral Fellow Full Time

    Thomas Jefferson University

    Philadelphia, Estados Unidos

    2009 - 2013

  •   Adjunct Investigator Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    2014 - 2014

  •   Associate professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    2015 - A la fecha

Experiencia Profesional

  •   Ingeniero en Alimentos Full Time

    Instituto de Calidad Alimentaria

    Chile

    2000 - 2001

Formación de Capital Humano


TEACHING
Currently, 2015-up to date:
University lecturer at Pontificia Universidad Católica de Chile (PUC)
Undergraduate: Cell Biology BIO141; Topics in Biochemistry BIO101B.
Graduate School Courses: Molecular Biology and Genetics BIO 4114, Cellular biology BIO4101, Molecular and cellular bases of neurobiology BIO4103, Cellular and molecular bases of pathology BIO4040.

Previous teaching:
School of Chemistry and Pharmaceutical Sciences, University of Chile. Course: Physiologic and Pathologic Chemistry II. Spring semester 2002 - 2004.
School of Medicine, University of Chile. Courses: Integrated Physiology – Chapter Digestive System. 2007-2008. Intracellular Calcium signaling, 2007. Molecular Biology. 2007. Sports physiology. 2007 - 2008.

RESEARCH SUPERVISION_Postdoctoral trainees
Carmer Silva, co-tutoring. Tutor: Dr. Nibaldo Inestrosa.
Wnt5 and mitochondrial fusion in hippocampal neurons

Paula Slater, co-tutoring. Tutor: Dr. Juan Larraín.
Mitochondrial Function In Axon And Spinal Cord Regeneration

RESEARCH SUPERVISION_PHD THESIS

Benjamín Cartes-Saavedra
Characterization of OPA1 disease-related mutations and their effect on mitochondrial fusion dynamics and cristae organization. Graduated in 2020.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Daniel Lagos
Efecto de mutaciones de Mitofusina 2 causantes de CMT2A en el equilibrio fusión-fisión y el metabolismo bioenergético mitocondrial, to be graduated in 2022.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Ana María Figueroa, co-tutoring. Tutor: Dr. Víctor Cortés
Efecto del desbalance de fosfolípidos sobre la morfología y función mitocondrial en adipocitos pardos Agpat2-/-. To be graduated in 2021.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Jeremy Salas, co-tutoring. Tutor: Dr. Hugo Olguin
Rol de la E3 ligasa Nedd4 en la regulación de Mitofagia en células miogénicas. To be graduated in 2020.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

RESEARCH SUPERVISION_UNDERGRADUATE THESIS
Erik López
Título: Intracellular Ca2+ homeostasis in Mid49/51 knockout cells. Graduated in 2020.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Josefa Macuada
Título: Mitochondrial DNA trafficking in OPA1 and MFN2 disease-related mutants. Graduated in 2020.
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Diego Troncoso
Título: "MUTACIÓN DELETÉREA EN LA PROTEÍNA DE FISIÓN MITOCONDRIAL MID49 ALTERA LA DINÁMICA MITOCONDRIAL ",
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE, Licenciatura/Bachillerato/Bachelor, 2018

Javier Cavieres
Título: CARACTERIZACIÓN DE LAS MITOCONDRIAS EN RESPUESTA AL DAÑO EN LA MÉDULA ESPINAL EN XENOPUS LAEVIS
Institución: PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE, Licenciatura/Bachillerato/Bachelor, 2018

UNDERGRADUATE THESIS CO-TUTORING UNIVERSITY OF CHILE (2002 – 2008).
Aedo, Paola
Título: "Estudio de distribución de proteínas de la maquinaria de fisión mitocondrial en células de músculo esquelético",
Institución: UNIVERSIDAD DE CHILE, Licenciatura/Bachillerato/Bachelor, 2006 Editar Eliminar

Parra, Valentina
Título: "Fisión Mitocondrial en la Muerte Celular Programada del Cardiomiocito",
Institución: UNIVERSIDAD DE CHILE, Magíster/Master/DEA, 2006 Editar Eliminar

Quiroga, Clara
Título: "NFkB protege de la muerte por apoptosis en cardiomiocitos expuestos a estrés hiperosmótico",
Institución: UNIVERSIDAD DE CHILE, Magíster/Master/DEA, 2004 Editar Eliminar

Criollo, Alfredo
Título: "Estrés oxidativo y activación del NF-kB en cardiomiocitos sometidos a estrés hiperosmótico, regulación por antioxidantes e IGF-1",
Institución: UNIVERSIDAD DE CHILE, Magíster/Master/DEA, 2003 Editar Eliminar

Examination of PhD Thesis: 8 on course PhD thesis (UChile, PUC).


Difusión y Transferencia


FUNDED RESEARCH PROJECTS

Role of mitochondrial inner membrane and mtDNA dynamics on mitochondrial bioenergetics in health and disease
Programa: FONDECYT
Rol: Inv. Principal | Folio/N°: 1191770 | 2019 - 2022

ROLE OF MITOCHONDRIA AND CAVEOLAE LIPID COMPOSITION ON AGPAT2-DEPENDENT LIPODYSTROPHY
Programa: FONDECYT
Rol: Coinvestigador(a) | Folio/N°: 1181214 | 2018 - 2021
Inv. principal: Dr. Vísctor Cortés.

Microscopio confocal de última generación: alta resolución espacial y temporal.
Programa: FONDEQUIP
Rol: Director(a) | Folio/N°: EQM170188 | 2017 - 2019

Role of mitochondria fusion proteins Opa-1 and Mfn2 specific mutations, in the pathogenesis of mitochondrial diseases.
Programa: FONDECYT
Rol: Inv. Principal | Folio/N°: 1150677 | 2015 - 2018

FISION MITOCONDRIAL Y SEÑALES DE CALCIO EN CULTIVOS PRIMARIOS DE MUSCULO ESQUELETICO SOMETIDOS A ESTIMULACION ELECTRICA
Programa: FONDECYT
Rol: Inv. Responsable | Folio/N°: 3070043 | 2007 - 2008

CURRENT ASSIGNMENTS
Academic Director of Advanced Microscopy Facility, PUC. Nov. 2015 - up to date.
Member of Study group session Biology 2, FONDECYT, CONICYT. Since May 2019.

MEMBERSHIPS
2007 - Present Biophysical Society.
2004 - Present Chilean Biological Society – Cell Biology Society

OUTREACH TO SOCIETY
Enero 2019.
Entrevista en Radio Pauta, Programa la Invención del Futuro. Tema: ¿qué son las enfermedades mitocondriales?
www.pauta.cl/programas/la-invencion-del-futuro/que-son-las-enfermedades-mitocondriales

Enero 2019.
Difusión de re-apretura, Unidad de Microscopía Avanzada UC. Directora VEisner.

Octubre 2018.
Participación en Mesa Redonda, Mujer y Academia.

Julio 2018
Visita a Colegio Fresno de Puente Alto, red de colegios Belén Educa, en el marco del Curso de Introducción a la Bioquímica, PUC, donde se presentaron trabajos de distintos científicos, recientemente publicados, a cursos 8vo a 2do medio. Coordinación del curso y dirección de actividades de exposición por parte de estudiantes de Bioquímica.
Bionoticia en Sitio Web Facultad Ciencias Biológicas, UC.

Jurado en Ferias Científicas Escolares Sociedad de Instrucción Primaria, Noviembre 2017.
Se adjunta noticia sitio web Facultad Ciencias Biológicas, UC.

Entrevista en Diario El Mercurio, 17/10/2017, acerca del Premio Nobel de Química por el desarrollo de la crio-microscopía electrónica. Dra. Verónica Eisner, Directora Unidad Microscopía Avanzada UC.

Noticia: Transitorios de calcio implicados en la actividad contráctil del corazón activan la comunicación mitocondrial, relativa la publicación: Eisner et al, PNAS 2017. Se adjunta noticia en sitio web Facultad Ciencias Biológicas, UC (marzo 2017). Esta noticia se encontró entre las más leídas del 2017 (ver anexo).

Estudiantes de Pregrado Laboratorio Dra. Eisner, Sergio Henríquez y Josefa Vial, participaron en Feria de Investigación de Pregrado UC, 2016. Se adjunta noticia del sitio web Facultad Ciencias Biológicas, UC.

Unidad de Microscopía Avanzada UC, dirigida por Dra. Eisner, se adjudica proyecto FONDEQUIP, para adquisición de microscopio confocal de última generación. Septiembre 2016.
Bionoticia en Sitio Web Facultad Ciencias Biológicas, UC.


Premios y Distinciones

  •   Oral Presentation Award

    Thomas Jefferson University

    Estados Unidos, 2012

    The Jefferson Postdoctoral Association and JCBS Office of Postdoctoral Affairs host the Postdoctoral Research Symposium. Participation is open only to Thomas Jefferson University postdoctoral fellows. The Symposium includes oral presentations, a poster session and an “Early Discoveries” poster session. The “Early Discoveries” category is designed to showcase postdocs that are new to TJU (less than 1 year), or that have started new projects (within 6 months). Each session is judged and monetary prizes awarded for the best presentations and posters in each category.

  •   Poster Presentation Award

    Thomas Jefferson University

    Chile, 2013

    The Jefferson Postdoctoral Association and JCBS Office of Postdoctoral Affairs host the Postdoctoral Research Symposium. Participation is open only to Thomas Jefferson University postdoctoral fellows. The Symposium includes oral presentations, a poster session and an “Early Discoveries” poster session. The “Early Discoveries” category is designed to showcase postdocs that are new to TJU (less than 1 year), or that have started new projects (within 6 months). Each session is judged and monetary prizes awarded for the best presentations and posters in each category.

  •   Young Bioenergeticist Award

    Biophysical Society

    Chile, 2014

    The Young Bioenergeticist Award is given to outstanding graduate students and postdocs as voted on by the members of the Bioenergetics Subgroup Council. The Young Bioenergeticist Award is based on the candidate's track record of accomplishments as well as the candidate's abstract that will be presented at the Biophysical Society Annual Meeting.

  •   Pew Latin America Fellow

    PEW LATIN AMERICAN FELLOWS PROGRAM IN BIOMEDICAL SCIENCES

    Chile, 2009

    The Pew Latin American Fellows Program in the Biomedical Sciences provides support for young scientists from Latin America to receive postdoctoral training in the United States. The program gives these individuals an opportunity to further their scientific knowledge by promoting exchange and collaboration between investigators in the United States and Latin America resulting in advances in research in Latin America.


 

Article (22)

Absence of AGPAT2 impairs brown adipogenesis, increases IFN stimulated gene expression and alters mitochondrial morphology
beta-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle
Inhibition of chymotrypsin-like activity of the proteasome by ixazomib prevents mitochondrial dysfunction during myocardial ischemia
Skeletal muscle excitation-metabolism coupling
A novel mechanism causing imbalance of mitochondrial fusion and fission in human myopathies
Down Syndrome Critical Region 1 Gene, Rcan1, Helps Maintain a More Fused Mitochondrial Network
Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
Mitochondrial fusion dynamics is robust in the heart and depends on calcium oscillations and contractile activity
Fatal infantile mitochondrial encephalomyopathy, hypertrophic cardiomyopathy and optic atrophy associated with a homozygous OPA1 mutation
Distribution and Apoptotic Function of Outer Membrane Proteins Depend on Mitochondrial Fusion
Mitochondrial fusion is frequent in skeletal muscle and supports excitation-contraction coupling
Interactions between sarco-endoplasmic reticulum and mitochondria in cardiac and skeletal muscle - pivotal roles in Calcium and reactive oxygen species signaling.
Switch from ER-mitochondrial to SR-mitochondrial calcium coupling during muscle differentiation.
ATP release due to Thy-1-integrin binding induces P2X7-mediated calcium entry required for focal adhesion formation
ATP release due to Thy-1-integrin binding induces P2X7-mediated calcium entry required for focal adhesion formation.
Mitochondria fine-tune the slow Ca2+ transients induced by electrical stimulation of skeletal myotubes
Parallel activation of Ca2+-induced survival and death pathways in cardiomyocytes by sorbitol-induced hyperosmotic stress
Changes in mitochondrial dynamics during ceramide-induced cardiomyocyte early apoptosis
Tumor necrosis factor-alpha activates nuclear factor-kappa B but does not regulate progesterone production in cultured human granulosa luteal cells
Hyperosmotic stress activates p65/RelB NF kappa B in cultured cardiomyocytes with dichotomic actions on caspase activation and cell death
Hyperosmotic stress-dependent NF kappa B activation is regulated by reactive oxygen species and IGF-1 in cultured cardiomyocytes
Aldose reductase induced by hyperosmotic stress mediates cardiomyocyte apoptosis - Differential effects of sorbitol and mannitol

Abstract (3)

Mitochondrial Ca2+ transients upon electrical stimulation of skeletal myotubes
Apoptosis induced by hyperosmotic stress
IGF-1 prevents NF kappa B activation but not reactive oxygen species generation by hyperosmotic stress in apoptotic cultured cardiac myocytes

Proyecto (14)

Role of mitochondrial inner membrane and mtDNA dynamics on mitochondrial bioenergetics in health and disease
ROLE OF MITOCHONDRIA AND CAVEOLAE LIPID COMPOSITION ON AGPAT2-DEPENDENT LIPODYSTROPHY
Microscopio confocal de última generación=> alta resolución espacial y temporal.
Role of mitochondria fusion proteins Opa-1 and Mfn2 specific mutations, in the pathogenesis of mitochondrial diseases.
Mitochondrial fusion in healthy and dystrophic skeletal muscle
Mitochondrial fusion-fission and motility dynamics in healthy and diseased muscle
Mitochondrial fusion-fission and motility dynamics in healthy and diseased muscle
DISTINCT PATTERNS OF CALCIUM SIGNALS REGULATE GENE EXPRESSION IN ADULT SKELETAL MUSCLE FIBERS
FISION MITOCONDRIAL Y SEÑALES DE CALCIO EN CULTIVOS PRIMARIOS DE MUSCULO ESQUELETICO SOMETIDOS A ESTIMULACION ELECTRICA
NFkB (Nuclear Factor kB) in the regulation of cardiac apoptosis
MECANISMOS DE TRANSDUCCION DE SENALES DEL IGF-1=>REGULACION DIFERENCIAL DE FACTORES TRANSCRIPCIONALES ASOCIADOS A LOS PROCESOS DE HIPERTROFIA Y APOPTOSIS DEL CARDIOMIOCITO
NFkB transcriptional factor regulation by hyperosmotic stress and IGF-1 in cardiac myocytes apoptosis
FISION MITOCONDRIAL Y SEÑALES DE CALCIO EN CULTIVOS PRIMARIOS DE MUSCULO ESQUELETICO SOMETIDOS A ESTIMULACION ELECTRICA
Mitochondrial fusion in healthy and dystrophic skeletal muscle

Review (2)

Mitochondrial dynamics in adaptive and maladaptive cellular stress responses
Mitochondrial Nanotunnels
26
Veronica Eisner

Associate Professor

Cellular and Molecular Biology

Pontificia Universidad Católica de Chile

Santiago, Chile

12
Sergio Lavandero

Director

Advanced Center for Chronic Diseases (ACCDiS)

UNIVERSIDAD DE CHILE, FAC CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

8
Mario Chiong

Profesor

Departamento de Bioquímica y Biología Molecular

Universidad de Chile

Santiago, Chile

7
Alfredo Criollo

Assistant professor

Research Institute in Dental Sciences

Facultad de Odontología, Universidad de Chile

Santiago, Chile

6
Clara Quiroga

Profesor Asistente Adjunto

División de Enfermedades Cardiovasculares

Pontificia Universidad Católica de Chile

Santiago, Chile

6
Enrique Jaimovich

Full Professor

ICBM

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

4
M. Cecilia Hidalgo

Professor

ICBM

Universidad de Chile

Santiago, Chile

2
claudio olea

Full Professor

Inorganica y Analitica

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Rodrigo Troncoso

Associate Professor

Instituto de Nutrición y Tecnología de los Alimentos

Santiago, Chile

2
Lisette Leyton

Full Professor

Cell and Molecular Biology

FACULTAD DE MEDICINA UNIVERSIDAD DE CHILE

Santiago, Chile

2
Zully Pedrozo

Assistant professor

Physiology and Biophysical

Universidad de Chile

SANTIAGO DE CHILE, Chile

1
Roberto Bravo

Assistant Professor

Institute of Nutrition and Food Technology (INTA), Universidad de Chile

Santiago, Chile

1
Rodrigo Herrera

Group Leader (staff member)

Combinatorial NeuroImaging Core Facility

Leibniz Institute for Neurobiology

Magdeburg, Alemania

1
Maria Tevy

investigadora invitada

INTA

Universidad de Chile

Santiago de Chile, Chile

1
Steffen Härtel

Full Professor

Instituto de Ciencias Biomédicas

University of Chile

Santiago, Chile

1
Valentina Parra

Profesora Asistente / Assistant Professor

Departamento de Bioquímica y Biología Molecular

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Mariana Casas

Assistant Professor

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Mauricio Castro

Investigador

Universidad Finis Terrae

Santiago , Chile

1
Juan Santibáñez

Professor of Research

Molecular Oncology

Institute for Medical Research

Belgrade, Serbia

1
Luis Barros

Principal Research

Laboratorio Biología Molecular

CENTRO DE ESTUDIOS CIENTÍFICOS

Valdivia, Chile

1
Gina Sanchez

Profesor Asociado

ICBM

UNIVERSIDAD DE CHILE, FAC MEDICINA , ICBM

Santiago, Chile

1
Ivonne Olmedo

Assistant Profesor

Programa de Fisiopatología

Universidad de Chile

Santiago, Chile

1
Paulina Donoso

Full Professor

Physiology and Biophysics

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Marcelo Kogan

Academico

Quimica Farmacologica y Toxicologica

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

1
Carlos Sepulveda

Investigador

Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile.

Santiago, Chile

1
Mauricio Henriquez

Profesor asistente

PROGRAMA DE FISIOLOGÍA Y BIOFíSICA

Universidad de Chile / Facultad de Medicina

Santiago, Chile

1
Ariel Contreras

assistance professor

Institute for Research in Dental Science

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Matias Monsalves

Investigador Postdoctoral

Universidad de O´Higgins

Rancagua, Chile

1
Luigi Devoto

PROFESOR TITULAR

INSTITUTO DE INVESTIGACIONES MATERNO INFANTIL,FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Rodrigo Troncoso

Assistant professor

Universidad de Chile, Instituto de Nutrición y Tecnología de los Alimentos

Santiago de Chile, Chile

1
Victor Cortes

Associate professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Reinaldo Figueroa

Instructor Adjunto

Facultad de Medicina y Ciencias de la Salud

Universidad Mayor de Chile

Santiago, Chile