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Enrique Brandan Siques

Profesor Titular

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

Líneas de Investigación


Molecular mechanisms that regulate skeletal muscle formation and how they are affected in skeletal muscular dystrophies.

Educación

  •  Cell Biology, PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE. Chile, 1985
  •  Biology, UNIVERSIDAD DE CHILE. Chile, 1979

Experiencia Académica

  •   Research Assistant Full Time

    VANDERBILT UNIVERSITY

    Nashville, Estados Unidos

    1979 - 1981

  •   PostDoctoral Fellow Full Time

    SAINT LOUIS UNIVERSITY

    Medicine

    Saint Louis, Estados Unidos

    1985 - 1986

  •   Postdoctoral fellow Full Time

    University of Massachusetts

    Medicine

    Worcester, Estados Unidos

    1986 - 1987

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Ciencias Biologicas

    Santiago, Chile

    1988 - 1992

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Ciencias Biologicas

    Santiago, Chile

    1992 - 1998

  •   Full Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Ciencias Biológicas

    Chile

    1989 - A la fecha

  •   Full Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Ciencias Biologicas

    Santiago, Chile

    1999 - A la fecha

Experiencia Profesional

  •   Research Associate Full Time

    Vanderbilt University

    Nashville, Estados Unidos

    1979 - 1981

  •   Visiting Assistant Research Professor Full Time

    St. Louis University

    Saint Louis, Estados Unidos

    1986 - 1987

  •   Assistant Professor Full Time

    University of Massachusetts

    Worcester, Estados Unidos

    1987 - 1988

  •   Profesor Asistente Full Time

    P. Universidad Católica de Chile

    Santiago, Chile

    1988 - 1992

  •   Profesor Asociado Full Time

    P. Universidad Católica de Chile

    Santiago, Chile

    1992 - 1998

  •   Profesor Titular Full Time

    P. Universidad Católica de Chile

    Santiago, Chile

    1999 - A la fecha

  •   Profesor/Investigador Full Time

    Fundación Ciencia & VIda

    Chile

    2019 - A la fecha

Formación de Capital Humano


Student: Winston Andrade
Career: Biology. Faculty of Biological Sciences, P. Universidad Católica de Chile.
Title: Composition, characterization and expression of proteoglycans in the extracellular matrix of rat skeletal muscle.
Duration: 1990-1991.

Student: Alejandro Campos.
Career: Biochemistry, Universidad de Concepción.
Title: Hydrophobic proteoglycans present on the surface of the skeletal muscle.
Duration: Octubre 1991-1993.

Student: Rebeca Nuñez.
Career: Biochemistry, University of Santiago.
Title: Differential expression of extracellular matrix sulfated macromolecules, during differentiation of the glands of the chick embryo.
Duration: 1991-1992.
Dr. Cecilia Köenig as Co-adviser.

Student: Jorge Cáceres.
Career: Biochemistry, Universidad de Concepción.
Title: Alzheimer: Interaction between ?-amyloid protein and extracellular matrix.
Duration: 1993-1994.

Student: Luis Fuentealba.
Career: Biochemistry, Universidad de Concepción.
Title: Synthesis, expression and function of syndecan-3 during myogenesis.
Duration: 1998-1999.

Student: Karin Alvarez.
Career: Biochemistry, Universidad Austral de Chile.
Title: Proteoglycan synthesis and expression in patients with Duchenne Muscular Dystrophy.
Duration: 2000-2001
.
Student: Rosario Calderón.
Career: Biochemistry, P. Universidad Católica de Chile.
Title: Expression and function of heparan sulfate proteoglycans in hippocampal cells: involvement of TGF-???
Duration: 2002-2003.

Student: Rebeca Droguett.
Career: Biochemistry, P. Universidad Católica de Chile.
Title: Two different mechanisms of regulation dependent signaling TGF-? during skeletal muscle differentiation.
Duration: 2003-2004.

Student: Jaime Gutierrez.
Career: Biochemistry, P. Universidad Católica de Chile.
Title: Changes in proteoglycan synthesis during conversion of myoblasts to osteoblasts in response to BMP-2.
Duration: 2004-2005.

Student: Pablo Cañón.
Career: Biochemistry.
Title: LPA participation and stability of the cytoskeleton in the induction of CTGF in muscle cells.
Duration: 2004-2005.

Student: Daniel Cabrera.
Career: Biochemistry, P. Universidad Católica de Chile.
Title: CTGF effect on proteoglycan synthesis in fibroblasts dystrophic.
Duration: 2006-2007.

With Claudio Cabello-Verrugio as Co-adviser
Student: Paula Painemal.
Career: Chemistry and Pharmacy, Universidad de Chile.
Title: Expression control of AT-1 receptor (for angiotensin II) in skeletal muscle.
Duration: 2010-2011.

Student: Camilo Riquelme.
Career: Molecular Biotechnology Engineering, Universidad de Chile.
Title: Interaction myoblast-extracellular matrix mediated by extracelular matrix is required for the expression of CTGF / CCN2.
Duration: 2014-2015.

Student: Juan Pablo Espinoza.
Career: Biotechnology, Universidad Santo Tomás. Santiago.
Title: Effect of SOD1 expression on muscle cells and fibroblasts: role in ALS.
Duration: Enerro 2016-julio 2017.

Professional Training

Student: Francisca Ruiz.
Career: Biochemistry, P. Universidad Católica de Chile.
Title: Isolation of myoblast clones transfected with syndecan-1.
Duration: 1996.

Graduate

Masters in Sciences.

Student: Francisco Melo.
Career: Master in Science, Biochemistry, P. Universidad Católica de Chile.
Title: Differential effect of extracellular matrix in the expression of early and late markers during differentiation of skeletal muscle cells.
Duration: 1993-1994.

Student: María José Villar.
Career: Master in Science, Biochemistry, P. Universidad Católica de Chile.
Title: Characterization of perlecan in myoblasts, association with the cell membrane and its role in cell adhesion processes.
Duration: 1995-1997.

Student: Soledad Cáceres.
Career: Master in Science, Biochemistry, P. Universidad Católica de Chile.
Title: Molecular and biochemical characterization of proteoglycans in mdx skeletal muscle of mice.
Duration: 1997-1999.

PhD Theses in Progress

Student: David Gonzalez.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Role-fibrotic factors and extracellular matrix components in the progression of amyotrophic lateral sclerosis (ALS).
Duration: 2015~2017.

Student: Osvaldo Contreras.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: TCF4 transcription factor and PDGF receptor alpha are key participants in the biology of skeletal muscle: role in myofibroblast differentiation and fibrogenic process triggered by TGF-?.
Duration: 2015~2017.

Student: Roger Valle.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Role of hipoxia in the expression of CTGF/CCN2 in pro-fibrotic cells.
Duration: 2016~2018.

Student: Javier Cerda
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Co-mentoring with Dr. Viviana Montecinos.
Title: Activación de la vía de señalización de VEGF-A en fibroblastos asociados a carcinoma y su papel en la agresividad tumoral en células cancerosas de próstata
Duration: 2017-2019

Student: Mauricio Castro
Career: Ph.D. in Medical Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Co-mentoring with Dr. Verónica Eisner.
Title: Rol de la fibrosis en la pérdida de la fusión mitocondrial del músculo esquelético en la distrofia muscular de Duchenne
Duration: 2017-2019

Completed PhD Theses

Student: Juan Larraín.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Heparan sulfate proteoglycans: Modulators of myogenesis.
Duration: 1995-1998.

Student: Juan Pablo Henríquez.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Identification and Function of Ligand for heparan sulfate proteoglycans During myogenesis.
Duration: 1997-2001.

Student: Cecilia Riquelme.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Modulation of skeletal muscle differentiation by decorina: Effect on TGF-? binding to its receptors.
Duration: 1997-2001.

Student: Hugo Olguín.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Modulation of embryonic myogenesis by proteoglycans.
Duration: 1998-2002.

Student: Nelson Osses.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Effect of extracellular matrix in determining the lineage of mesodermal cells Duration: 1998-2002.

Student: Juan Carlos Casar, M.D.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Participation of proteoglycans in skeletal muscle regeneration in mouse.
Duration: 1999-2003.

Student: Claudia Hurtado.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Biglycan: Participation in skeletal muscle differentiation, modulating the bioavailability of TGF-? and factors that regulate their expression.
Duration: 2002-2005.

Student: Claudio Cabello-Verrugio.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: TGF-? dependent signaling during myogenesis. Participation of decorin and LRP.
Duration: 2004-2007.

Student: Valeria Mezzano, M.D.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: CTGF involvement in skeletal muscle fibrosis.
Duration: 2004-2007.

Student: Cecilia Vial.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: CTGF involvement in skeletal muscle fibrosis during myogenesis and its modulation by proteoglycans.
Duration: 2005-2008.

Student: Rebeca Droguett.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Participation of TGF-? receptors during myogenesis.
Duration: 2006-2009.

Student: Jaime Gutierrez.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Regulation of FGF-2 signaling by Glypican-1 during muscle differentiation.
Duration: 2007-2009.

Student: Daniel Cabrera.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Role of NFkB in skeletal muscle fibrosis induced by exercise.
Duration: 2010-2012.

Student: María Gabriela Morales.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Biological Role of Connective Tissue Growth Factor (CTGF / CCN2) in Skeletal Muscle Fibrosis.
Duration: 2009-2012.

Student: María José Acuña.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Role of the renin-angiotensin system in skeletal muscle fibrosis.
Duration: 2010-2013.

With Pascal Vigey as Co-Adviser
Student: Gozalo Córdova.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile and Doctorat de Biologie Cellulaire et Moléculaire, Université Pierre et Marie Curie, Paris, France.
Title: Role of CTGF and TNF on fibrosis in muscular dystrophy.
Duration: 2011-2014.

Student: Héctor A. Ramirez.
Career: Ph.D. in Biological Sciences, mention in Cell and Molecular Biology, P. Universidad Católica de Chile.
Title: Angiotensina (1-7) promueve la regeneración muscular aumentando la expresión de inos en monocitos pro inflamatorios y promoviendo la expansión de células satélites de forma no dependiente
Duration: 2014-2016.


Premios y Distinciones

  •   "Program Science in developing countries".

    NATIONAL SCIENCE FOUNDATION

    Estados Unidos, 1988

    "Program Science in developing countries". Título: Proteoglycan Sulfotransferases.

  •   Howard Hughes International Research Scholar

    Howard Hughes Medical Institute

    Estados Unidos, 1997

    - Howard Hughes International Research Scholar

  •   “Best Teacher”

    FEUC

    Chile, 1997

    Federación Estudiantes (FEUC), P. Universidad Católica de Chile

  •   Presidential Chair in Sciences

    Presidencia de la República

    Chile, 1998

    - Cátedra Presidencial en Ciencias (1998-2000)

  •   - Award for “Best Undergraduate Thesis” Mr. Luis Fuentealba. (Enrique Brandan, Advisor)

    Fundación Chilena para la Biología Celular

    Chile, 2000

    Premio a “La mejor Tesis de Pregrado” Sr. Luis Fuentealba, (Enrique Brandan, Director)

  •   Award for “Best PhD Thesis”. Dr. Juan Larrain (Enrique Brandan, Director)

    Fundación Chilena para la Biología Celular

    Chile, 2000

    Premio a “La mejor Tesis de Doctorado”. Sr. Juan Larraín, (Enrique Brandan, Director)

  •   Howard Hughes International Research Scholar

    Howard Hughes Medical Institute

    Estados Unidos, 2002

    Howard Hughes International Research Scholar

  •   - Member of the Chilean Academy of Sciences

    Academia Chilena de Ciencias

    Chile, 2008

    Miembro Correspondiente de la Academia Chilena de Ciencias

  •   Best Teacher

    Federación Estudiantes (FEUC), P. Universidad Católica de Chile

    Chile, 2007

    “Mejor Profesor”

  •   Recognition Award for Excellence in Teaching, P. Universidad Católica de Chile.

    Vice Rectoría Académica, P. Universidad Católica de Chile

    Chile, 2007

    Premio de Reconocimiento a la Excelencia Docente

  •   Recognition for serving the P. Universidad Católica de Chile for 25 years

    P. Universidad Católica de Chile

    Chile, 2013

    Reconocimiento a los 25 años de servicio a la P. Universidad Católica de Chile

  •   Innovation Award. Copec-UC

    Fundación Copec-UC

    Chile, 2014

    Innovation Award

  •   - Miembro Académico de la Academia de Ciencias de América Latina (ACAL)

    Academia de Ciencias de América Latina (ACAL)

    Venezuela, 2017

    Miembro Académico de la Academia de Ciencias de América Latina (ACAL)


 

Article (134)

Angiotensin-(1-7) Prevents Lipopolysaccharide-Induced Autophagy via the Mas Receptor in Skeletal Muscle
Role of hypoxia in skeletal muscle fibrosis: Synergism between hypoxia and TGF-? signaling upregulates CCN2/CTGF expression specifically in muscle fibers
TGF-beta-driven downregulation of the transcription factor TCF7L2 affects Wnt/beta-catenin signaling in PDGFR alpha(+) fibroblasts
Cross-talk between TGF-beta and PDGFR alpha signaling pathways regulates the fate of stromal fibro-adipogenic progenitors
Denervation-induced skeletal muscle fibrosis is mediated by CTGF/CCN2 independently of TGF-beta
Denervation-induced skeletal muscle fibrosis is mediated by CTGF/CCN2 independently of TGF-β.
Blockade of Bradykinin receptors worsens the dystrophic phenotype of mdx mice: differential effects for B1 and B2 receptors
Blockade of Bradykinin receptors worsens the dystrophic phenotype of mdx mice=> differential effects for B1 and B2 receptors.
Expression of CTGF/CCN2 in response to LPA is stimulated by fibrotic extracellular matrix via the integrin/FAK axis
Expression of CTGF/CCN2 in response to LPA is stimulated by fibrotic extracellular matrix via the integrin/FAK axis.
Nilotinib impairs skeletal myogenesis by increasing myoblast proliferation
Nilotinib impairs skeletal myogenesis by increasing myoblast proliferation.
The inhibition of CTGF/CCN2 activity improves muscle and locomotor function in a murine ALS model
The inhibition of CTGF/CCN2 activity improves muscle and locomotor function in a murine ALS model.
The pro-fibrotic connective tissue growth factor ( CTGF/CCN2) correlates with the number of necrotic-regenerative foci in dystrophic muscle
The pro-fibrotic connective tissue growth factor (CTGF/CCN2) correlates with the number of necrotic-regenerative foci in dystrophic muscle.
ALS skeletal muscle shows enhanced TGF-beta signaling, fibrosis and induction of fibro/adipogenic progenitor markers
Andrographolide Ameliorates Inflammation and Fibrogenesis and Attenuates Inflammasome Activation in Experimental Non-Alcoholic Steatohepatitis
Fibro/adipogenic progenitors safeguard themselves: a novel mechanism to reduce fibrosis is discovered
The pro-fibrotic connective tissue growth factor (CTGF/CCN2) correlates with the number of necrotic-regenerative foci in dystrophic muscle
Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy in mice via its receptor, Mas
Connective tissue cells expressing fibro/adipogenic progenitor markers increase under chronic damage: relevance in fibroblast-myofibroblast differentiation and skeletal muscle fibrosis
Diet-Induced Nonalcoholic Fatty Liver Disease Is Associated with Sarcopenia and Decreased Serum Insulin-Like Growth Factor-1
Wnt signaling pathway improves central inhibitory synaptic transmission in a mouse model of Duchenne muscular dystrophy
Angiotensin-(1-7) decreases skeletal muscle atrophy induced by angiotensin II through a Mas receptor-dependent mechanism
Endotoxin-induced skeletal muscle wasting is prevented by angiotensin-(1-7) through a p38 MAPK-dependent mechanism
RECK-Mediated beta 1-Integrin Regulation by TGF-beta 1 Is Critical for Wound Contraction in Mice
SMAD3 and SP1/SP3 Transcription Factors Collaborate to Regulate Connective Tissue Growth Factor Gene Expression in Myoblasts in Response to Transforming Growth Factor
The angiotensin-(1-7)/Mas axis reduces myonuclear apoptosis during recovery from angiotensin II-induced skeletal muscle atrophy in mice
Transforming growth factor type-beta inhibits Mas receptor expression in fibroblasts but not in myoblasts or differentiated myotubes; Relevance to fibrosis associated to muscular dystrophies
ACE2 Is Augmented in Dystrophic Skeletal Muscle and Plays a Role in Decreasing Associated Fibrosis
ACE2 is augmented in dystrophic skeletal muscle and plays a role in decreasing associated fibrosis.
Andrographolide attenuates skeletal muscle dystrophy in mdx mice and increases efficiency of cell therapy by reducing fibrosis
Andrographolide attenuates skeletal muscle dystrophy in mdx mice and increases efficiency of cell therapy by reducing fibrosis.
Glypican-1 regulates myoblast response to HGF via Met in a lipid raft-dependent mechanism: effect on migration of skeletal muscle precursor cells
Glypican-1 regulates myoblast response to HGF via Met in a lipid raft-dependent mechanism=> effect on migration of skeletal muscle precursor cells.
Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophy
Restoration of muscle strength in dystrophic muscle by angiotensin-1-7 through inhibition of TGF-beta signalling
Restoration of muscle strength in dystrophic muscle by angiotensin-1-7 through inhibition of TGF-β signalling.
Wnt Signaling in Skeletal Muscle Dynamics: Myogenesis, Neuromuscular Synapse and Fibrosis
Inhibition of the angiotensin-converting enzyme decreases skeletal muscle fibrosis in dystrophic mice by a diminution in the expression and activity of connective tissue growth factor (CTGF/CCN-2)
Mice Long-Term High-Fat Diet Feeding Recapitulates Human Cardiovascular Alterations: An Animal Model to Study the Early Phases of Diabetic Cardiomyopathy
Reducing CTGF/CCN2 slows down mdx muscle dystrophy and improves cell therapy
Role of proteoglycans in the regulation of the skeletal muscle fibrotic response.
Transforming growth factor type beta 1 increases the expression of angiotensin II receptor type 2 by a SMAD- and p38 MAPK-dependent mechanism in skeletal muscle
Transforming growth factor type beta 1 increases the expression of angiotensin II receptor type 2 by a SMAD- and p38 MAPK-dependent mechanism in skeletal muscle.
Angiotensin II receptor type 1 blockade decreases CTGF/CCN2-mediated damage and fibrosis in normal and dystrophic skeletal muscles
Angiotensin II receptor type 1 blockade decreases CTGF/CCN2-mediated damage and fibrosis in normal and dystrophic skeletal muscles.
Angiotensin II-induced pro-fibrotic effects require p38MAPK activity and transforming growth factor beta 1 expression in skeletal muscle cells
The Internal Region Leucine-rich Repeat 6 of Decorin Interacts with Low Density Lipoprotein Receptor-related Protein-1, Modulates Transforming Growth Factor (TGF)-beta-dependent Signaling, and Inhibits TGF-beta-dependent Fibrotic Response in Skeletal Muscles
Connective tissue growth factor induction by lysophosphatidic acid requires transactivation of transforming growth factor type beta receptors and the JNK pathway
CTGF/CCN-2 over-expression can directly induce features of skeletal muscle dystrophy
Decorin Interacts with Connective Tissue Growth Factor (CTGF)/CCN2 by LRR12 Inhibiting Its Biological Activity
Fibrotic response induced by angiotensin-II requires NAD(P)H oxidase-induced reactive oxygen species (ROS) in skeletal muscle cells
A Novel Mechanism of Sequestering Fibroblast Growth Factor 2 by Glypican in Lipid Rafts, Allowing Skeletal Muscle Differentiation
Syndecan-4 and beta 1 integrin are regulated by electrical activity in skeletal muscle: Implications for cell adhesion
TGF-beta receptors, in a Smad-independent manner, are required for terminal skeletal muscle differentiation
Uptake of Tritiated Liquids by Individual Breakfast Cereal Flakes
Inhibition of extracellular matrix assembly induces the expression of osteogenic markers in skeletal muscle cells by a BMP-2 independent mechanism
Matrix Metalloproteinase-2-deficient Fibroblasts Exhibit an Alteration in the Fibrotic Response to Connective Tissue Growth Factor/CCN2 because of an Increase in the Levels of Endogenous Fibronectin
CTGF Inhibits BMP-7 Signaling in Diabetic Nephropathy
Heparin activates Wnt signaling for neuronal morphogenesis
Novel regulatory mechanisms for the proteoglycans decorin and biglycan during muscle formation and muscular dystrophy.
Skeletal muscle cells express the profibrotic cytokine connective tissue growth factor (CTGF/CCN2), which induces their dedifferentiation
A novel modulatory mechanism of transforming growth factor-ss signaling through decorin and LRP-1
Adenovirus-mediated hepatic syndecan-1 overexpression induces hepatocyte proliferation and hyperlipidaemia in mice
Constitutively activated dystrophic muscle fibroblasts show a paradoxical response to TGF-beta and CTGF/CCN2.
Betaglycan induces TGF-beta signaling in a ligand-independent manner, through activation of the p38 pathway
Changes in secreted and cell associated proteoglycan synthesis during conversion of myoblasts to osteoblasts in response to bone morphogenetic protein-2: Role of decorin in cell response to BMP-2
Extracellular proteoglycans modify TGF-beta bio-availability attenuating its signaling during skeletal muscle differentiation
Increase in decorin and biglycan in Duchenne Muscular Dystrophy: role of fibroblasts as cell source of these proteoglycans in the disease
Sulfation is required for bone morphogenetic protein 2-dependent Id1 induction
The low density lipoprotein receptor-related protein functions as an Endocytic receptor for decorin
Transforming growth factor beta (TGF-beta) signaling is regulated by electrical activity in skeletal muscle cells - TGF-beta type I receptor is transcriptionally regulated by myotube excitability
Biglycan is a new extracellular component of the Chordin-BMP4 signaling pathway
Caenorhabditis elegans syndecan (SDN-1) is required for normal egg laying and associates with the nervous system and the vulva
Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration
Heparan sulfate proteoglycans are increased during skeletal muscle regeneration: requirement of syndecan-3 for successful fiber formation
Structural and functional organization of synaptic acetylcholinesterase
The formation of skeletal muscle myotubes requires functional membrane receptors activated by extracellular ATP
Transient up-regulation of biglycan during skeletal muscle regeneration: delayed fiber growth along with decorin increase in biglycan-deficient mice
Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation - Cloning of murine betaglycan gene promoter and its modulation by myoD, retinoic acid, and transforming growth factor-beta
Inhibition of myoblast migration via decorin expression is critical for normal skeletal muscle differentiation
Augmented synthesis and differential localization of heparan sulfate proteoglycans in Duchenne muscular dystrophy
ECM is required for skeletal muscle differentiation independently of muscle regulatory factor expression
Extracellular matrix histone H1 binds to perlecan, is present in regenerating skeletal muscle and stimulates myoblast proliferation
Antisense inhibition of decorin expression in myoblasts decreases cell responsiveness to transforming growth factor beta and accelerates skeletal muscle differentiation
Expression and localization of proteoglycans during limb myogenic activation
Postnatal Msx1 expression pattern in craniofacial, axial, and appendicular skeleton of transgenic mice from the first week until the second year
Synthesis of proteoglycans is augmented in dystrophic mdx mouse skeletal muscle
Antisense inhibition of syndecan-3 expression during skeletal muscle differentiation accelerates myogenesis through a basic fibroblast growth factor-dependent mechanism.
Interaction of skeletal muscle cells with collagen type IV is mediated by perlecan associated with the cell surface.
Decorin core protein fragment Leu155-Val260 interacts with TGF-beta but does not compete for decorin binding to type I collagen.
Syndecan-1 expression inhibits myoblast differentiation through a basic fibroblast growth factor-dependent mechanism.
Ethodin=> pharmacological evidence of the interaction between smooth muscle and mast cells in the myometrium.
Expression of perlecan, a proteoglycan that binds myogenic inhibitory basic fibroblast growth factor, is down regulated during skeletal muscle differentiation.
Interaction between Alzheimer's disease beta A4 precursor protein (APP) and the extracellular matrix=> evidence for the participation of heparan sulfate proteoglycans.
Syndecan-1 expression is down-regulated during myoblast terminal differentiation. Modulation by growth factors and retinoic acid.
Extracellular matrix is required for skeletal muscle differentiation but not myogenin expression.
Significantly reduced expression of the proteoglycan decorin in Alzheimer's disease fibroblasts.
Synthesis and processing of glypican during differentiation of skeletal muscle cells.
Differentiation of oxyntic cells and cell-matrix interactions during avian gastric gland morphogenesis.
Proteoglycans in skeletal muscle.
A lipid-anchored heparan sulfate proteoglycan is present in the surface of differentiated skeletal muscle cells. Isolation and biochemical characterization.
Decorin is specifically solubilized by heparin from the extracellular matrix of rat skeletal muscles.
Extracellular matrix components and amyloid in neuritic plaques of Alzheimer's disease.
Isolation and partial characterization of cholesterol pronucleating hydrophobic glycoproteins associated to native biliary vesicles.
A high molecular weight proteoglycan is differentially expressed during development of the mollusc Concholepas concholepas (Mollusca; Gastropoda; Muricidae).
Axonal sprouting induced in the sciatic nerve by the amyloid precursor protein (APP) and other antiproteases.
Decorin, a chondroitin/dermatan sulfate proteoglycan is under neural control in rat skeletal muscle.
Isolation and purification of human biliary vesicles with potent cholesterol-nucleation-promoting activity.
Isolation of proteoglycans synthesized by rat heart=> evidence for the presence of several distinct forms.
Isolation and characterization of rat skeletal muscle proteoglycan decorin and comparison with the human fibroblast decorin.
The proteoglycan decorin is synthesized and secreted by differentiated myotubes.
[Biotechnological aspects in "loco" larvae].
Differential association and distribution of acetyl- and butyrylcholinesterases within rat liver subcellular organelles.
Motor nerve regulates muscle extracellular matrix proteoglycan expression.
Different membrane-bound forms of acetylcholinesterase are present at the cell surface of hepatocytes.
Differential association of rat liver heparan sulfate proteoglycans in membranes of the Golgi apparatus and the plasma membrane.
Purification of rat liver N-heparan-sulfate sulfotransferase.
Co-solubilization of asymmetric acetylcholinesterase and dermatan sulfate proteoglycan from the extracellular matrix of rat skeletal muscles.
Glycosaminoglycan composition of electric organ basement membranes.
Isolation of the heparan sulfate proteoglycans from the extracellular matrix of rat skeletal muscle.
Heparin-acetylcholinesterase interaction=> Specific detachment of class I-A forms and binding of class I and II-A forms to heparin-agarose.
The synaptic form of acetylcholinesterase binds to cell-surface heparan sulfate proteoglycans.
Anchorage of collagen-tailed acetylcholinesterase to the extracellular matrix is mediated by heparan sulfate proteoglycans.
Binding of the asymmetric forms of acetylcholinesterase to heparin.
Isolation and characterization of coated vesicles from rat liver.
Golgi complex function in the excretion of renal kallikrein (41502).
Orientation and role of nucleosidediphosphatase and 5'-nucleotidase in Golgi vesicles from rat liver.
Subcellular fractionation studies on the organization of fatty acid oxidation by liver peroxisomes.
Salt loading effect on renal kallikrein activity in rat.
Increase in decorin and biglycan in Duchenne Muscular Dystrophy=> role of fibroblasts as cell source of these proteoglycans in the disease.
Transforming growth factor type beta 1 increases the expression of angiotensin II receptor type 2 by a SMAD- and p38 MAPK-dependent mechanism in skeletal muscle.

Abstract (5)

NFkB inhibition by Andrographolide ameliorates inflammation and fibrogenesis through inflammasome substrate depletion in experimental Non-Alcoholic Steatohepatitis (NASH)
Diet-induced nonalcoholic fatty liver disease is associated with sarcopenia and decreased serum insulin growth factor-1
Lipopolysaccharide-induced skeletal muscle atrophy is prevented by Angiotensin (1-7) via Mas receptor.
Effects of andrographolide in experimental non-alcoholic steatohepatitis
Human platelet activation/secretion and a membrane-associated urokinase-like activity induce the activation of the latent form of platelet transforming growth factor-beta 1 (TGF-beta 1)

BookWhole (1)

Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy

EditorialMaterial (1)

Heparan sulfate provides a mechanism to respond to FGFR2b and control regenerative expansion

Errata (1)

Transforming growth factor type- inhibits Mas receptor expression in fibroblasts but not in myoblasts or differentiated myotubes; Relevance to fibrosis associated to muscular dystrophies (vol 42, pg 111, 2015)

Patent (4)

Methods for Treatment Motor Neuron Diseases
1. Methods for Treatment of Muscular Dystrophy. Estados Unidos
Pharmaceutical compositions containing the agonist of receptor Mas for the treatment of degenerative muscle diseases.
Pharmaco-Cellular Therapeutic Method for the Treatment of Muscular Dystrophies

Proyecto (19)

Participation of mesenchymal progenitors in the initiation of skeletal muscle fibrosis: Effect of TGFβ/CTGF/PDGF and cofactors on fibroblasts’ ability to modulate myogenesis
Novel Antifibrotic Role of the ACE2/Angiotensin 1-7/Mas Axis in Skeletal Muscular Dystrophies
Centro de Envejecimiento y Regeneración (CARE)
CTGF the factor involved in fibrosis development in DMD
Droga Botánica para el tratamiento de enfermedades crónicas asociadas con fibrosis y de alta incidencia
Skeletal muscle formation, function and disease.
DMD fibrosis: Role of CTGF, LRP and proteoglycans
Skeletal muscle formation, function and disease
Biological Function of Proteoglycans during Myogenesis
Function of proteoglycans during skeletal muscle differentiation
Decorin as a modulator of TGF-beta activity on skeletal muscle differentiation
Function heparan sulfate proteoglycans during myogenesis
Papel de los proteoglicanes en la formación de la placa neurítica, el crecimiento neuronal y el eventual diagnóstico de la enfermedad de Alzheimer
Role of the proteoglycans in the organization of the extracellular matrix and their effect on skeletal muscle differentiation.
Skeletal Muscle hydrophobic Proteoglycans
Biochemicals factors that control sulphation in muscle cells.
Expression and regulation of proteoglycans of the extracellular matrix of muscle and evaluation in skeletal muscle disorders.
Proteoglycan Sulfotransferases.
Millennium Institute for Fundamental and Applied Biology

Review (6)

HIF-hypoxia signaling in skeletal muscle physiology and fibrosis
CTGF/CCN2 from Skeletal Muscle to Nervous System: Impact on Neurodegenerative Diseases
Angiotensins as therapeutic targets beyond heart disease
Role of proteoglycans in the regulation of the skeletal muscle fibrotic response
Role of skeletal muscle proteoglycans during myogenesis
Novel regulatory mechanisms for the proteoglycans decorin and biglycan during muscle formation and muscular dystrophy
123
Enrique Brandan

Profesor Titular

Biología Celular y Molecular

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

29
Claudio Cabello

Full Professor

Ciencias Biologicas

UNIVERSIDAD ANDRES BELLO

Santiago, Chile

20
Daniel Cabrera

Investigador Adjunto

Gastroenterología

Pontificia Universidad Catolica de Chile

santiago, Chile

13
Jaime Gutierrez

Associate Professor and Researcher

Tecnología Médica

Universidad San Sebastián

Santiago, Chile

12
María Acuña

Directora de Investigación, académico e investigador

Universidad Bernardo O´Higgins

Santiago, Chile

8
Felipe Simon

Profesor Titular

Department of Biological Sciences

UNIVERSIDAD ANDRES BELLO FAC DE CS BIOLOGICAS, DPTO DE CS BIOLOGICAS

Santiago, Chile

8
Maria Vial

Associate Professor/Profesor Asociado

Instituto de Ciencias e Innovación de Medicina

UNIVERSIDAD DEL DESARROLLO

Santiago, Chile

8
Nelson Osses

Associated Professor

Institute of Chemistry

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

6
Hugo Olguin

Profesor Asociado

Biología Celular y Molecular

Pontificia Universidad Católica de Chile

Santiago, Chile

5
MARCO ARRESE

FULL PROFESSOR

GASTROENTEROLOGY

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
Nelson Osses

Profesor Titular

Instituto de Química

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

5
Hugo Olguin

Profesor Asociado

Biología Celular y Molecular

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
Carlos Vio

Profesor

Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
Nibaldo Inestrosa

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
Daniela Rebolledo

Académico

Centro de Excelencia en Biomedicina de Magallanes

Punta Arenas, Chile

4
ricardo fadic

Jefe de Division

Division de Neurociencias

DEPARTAMENTO DE NEUROLOGÍA, FACULTAD DE MEDICINA, P. UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

4
David González

Investigador postdoctoral

Institut des Neurosciences de Montpellier (INM)

Montpellier, Francia

4
JUAN PABLO HENRIQUEZ

Associate Professor

Cell Biology

UNIVERSIDAD DE CONCEPCIÓN, FACULTAD DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGÍA CELULAR

Concepción, Chile

3
Margarita Pizarro

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

3
Yaneisi Vazquez

Directora técnica de proyectos

Genética Molecular

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Johanna Ábrigo

Bioquímico

Centro de Bioinformática y Biología Integrativa

Santiago, Chile

2
Alejandra Alvarez

Full professor

Cell and Molecular Biology

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE, FACULTAD DE CIENCIAS BIOLÓGICAS

Santiago, Chile

2
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE - ESCUELA DE MEDICINA - DEPTO DE GASTROENTEROLOGÍA

Santiago, Chile

2
Gonzalo Ugarte

Asistente de Investigación

Biología

UNIVERSIDAD DE SANTIAGO DE CHILE

Santiago, Chile

2
Carlos Cespedes

Tecnologo Medico

Fisiologia

P. Universidad Católica de Chile

Santiago, Chile

2
Rebeca Aldunate

Profesor Titular

Ciencias

UNIVERSIDAD SANTO TOMÁS

Santiago, Chile

2
Juan Agustín Larraín

Profesor Titular

Biologia Celular y Molecular

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Francisco Melo

Profesor Asociado

Genetica Molecular y Microbiologia

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

2
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

2
JUAN VILLENA

ASSOCIATE PROFESSOR

BIOMEDICAL SICIENCES

FACULTA DE MEDICINA. UNIVERSIDAD DE VALPARAISO

VALPARAISO, Chile

1
Sergio Mezzano

Catedratico

Medicina

Universidad Austral de Chile

Valdivia, Chile

1
Francisco Barrera

Profesor Asociado

Gastroenterología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Andrea Leiva

Profesor Asociado

Facultad de Ciencias de la Salud

Universidad San Sebastián

Santiago, Chile

1
Victor Cortes

Associate professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Maria Paz Marzolo

Profesor Titular

Departamento de Biología Celular y Molecular

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE, FAC. CIENCIAS BIOLOGICAS, DEPTO. BIOLOGIA

Santiago, Chile

1
LUIS SOBREVIA

Professor

Division of Obstetrics and Gynaecology

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

1
Olga Panes

Technical Director/ Associated Research

Hematology-Oncology

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Juan Saez

Professor

Instituto de Neurociencias

Universidad de Valparaiso

Valparaíso, Chile

1
Juan Oyarzún

Investigador postdoctoral

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Juan Rivera

Investigador

Instituto Milenio de Inmunología e Inmunoterapia

Santiago, Chile

1
Pedro Cisternas

Investigador Asociado

Biología Celular

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Marco Fuenzalida

Full Professor

Physiology

UNIVERSIDAD DE VALPARAÍSO

Valparaíso, Chile

1
Sebastián Calligaris

Cordinador de Estudios Clínicos

Clínica Alemana

Santiago, Chile

1
Rosana Muñoz

postdoctorante

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Mirentxu Iruretagoyena

Jefe Seccion Inmunologia

Laboratorio Clinico

Clinica Alemana de Santiago

Santiago, Chile

1
Alicia Nogueras

Profesor Asociado Adjunto

Departamento de Biología Celular y Molecular

P. UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Felipe Court

Full Professor and Director

Center for Integrative Biology

Universidad Mayor

Huechuraba, Chile

1
Loreto Cuitiño

Investigador Asociado

Oftalmología

Hospital Clínico Universidad de Chile

229788866, Chile

1
Brigitte van Zundert

Full Professor

UNIVERSIDAD ANDRÉS BELLO, CENTRO DE INVESTIGACIONES BIOMÉDICAS

Santiago, Chile

1
Pablo Cañón

Académico Regular

Escuela de Agronomía

Universidad Mayor de Chile

Santiago, Chile