Man

Juan Carlos Saez Carreño

Professor

Universidad de Valparaiso

Valparaíso, Chile

Líneas de Investigación


Regulation and function of gap junctions and hemichannels formed by connexins or pannexins in cells of the brain and skeletal muscles under normal and pathological conditions.

Educación

  •  Neuroscience, ALBERT EINSTEIN COLLEGE OF MEDICINE. Estados Unidos, 1986
  •  Biochemist, UNIVERSIDAD DE CONCEPCION. Chile, 1979

Experiencia Académica

  •   Instructor Full Time

    UNIVERSIDAD DE CONCEPCION

    Biological Sciences

    Concepcion, Chile

    1979 - 1983

  •   Assistant Professor Full Time

    ALBERT EINSTEIN COLLEGE OF MEDICINE

    New York, Estados Unidos

    1987 - 1993

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    1993 - 1994

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    1995 - 2002

  •   Full Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Biological Sciences

    Santiago, Chile

    2003 - 2017

  •   Full Professor Full Time

    UNIVERSIDAD DE VALPARAISO

    Facultad de Ciencias

    Valparaiso, Chile

    2017 - A la fecha

Experiencia Profesional

  •   INstructor Full Time

    Universidad de Concepcion

    Concepcion, Chile

    1979 - 1983

  •   Assistant Professor Full Time

    Albert Instein College of Medicine

    New York, Estados Unidos

    1987 - 1993

  •   Assistant Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    1993 - 1994

  •   Associate Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    1994 - 2002

  •   Professor Full Time

    Pontificia Universidad Católica de Chile

    Santiago, Chile

    2003 - A la fecha

  •   Professor Full Time

    Universidad de Valparaiso

    Valparaiso, Chile

    2017 - A la fecha

Formación de Capital Humano


7 postoral fellows
-Dr. Iván Bravo. Involvement of connexin hemichannles expressed by glial cells in depression. (2018-present).
-Dr. Iván Barría. Role of connexin-based channels expressed by glia in the cochlea under inflammatory conditions. (2018-present)
-Dr. Rosalba Escamilla. Role of hemichannels in oligodendrocytes of neuroinflammatory diseases.(2014-present).
-2014-2017. Dra. Elisa Balboa. Proyecto Fondecyt. Relevancia del estrés oxidativo, la disfunción mitocondrial y la expresión de conexinas en la inducción de atrofia muscular por
dexamatasona.
-Dr. Carlos Puebla. FONDECYT. (3130662). "Regulation glucose transport through its specific transporters and hemichannels by essential fatty acids in the small intestine".
-Dr. Adam Aguirre. FONDECYT. (3130632)."Involvement of hemichannels in glia pre-activation by auditory stress to inflammation promoted by poly (I:C) and consequences on
neuronal arborization and survival".
-Dr. Jose Luis Vega. FONDECYT.(3120006). “Regulation of connexin-43 hemichannels properties by PKC, PKA and MAP kinases”.

34 graduate students and 6 undergraduate students
- 2020 Presente. Claudia García. Doctorado en Neurociencias, Instituto de Neurociencias. Universidad de Valparaíso. Mejoramiento del tratamiento actual de la epilepsia
considerando la neuroinflamación mediada por hemicanales
- 2012-2020. Ximena López. Doctor Ciencias Fisiológicas, PUC. Papel de hemicanales formadospor Panexina 1 en la inhibición de la activación de linfocitos T CD4+ por
adenosina
- 2011-present. Bruno Cisterna.Doctor Ciencias Fisiológicas, PUC. "Role of connexin hemichannels in muscle atrophy induced by denervation".
- 2012-present. Elsa Fritz,(Co-mentor with Dr. Jorge Campusano) Doctor Ciencias Fisiológicas, PUC. "Role of innexins on the coordinated circadian of behavior in Drosophila
melanogaster".
- 2013-present. Gonzalo Gomez, Doctor Ciencias Fisiológicas, PUC."The R hoA/ROCK pathway regulates the connexin43 hemichannel and gap junctional channel activity in
angiotensin-induce kidney injury.
- 2013-present. Dusan Recordon. (co-mentor with Dr. Gareth Owen). Doctor Ciencias Fisiológicas, PUC. "Purinergic signaling in the vasculogenesis-like process".
- 2009-present. Carola Marturana, Doctor Ciencias Fisiológicas, PUC. "Involvement of pannexin1 hemichannels of oligodendrocytes in amyelinization during prenatal stress".
- 20111-present Anibal Vargas, Doctor Ciencias Fisiológicas, PUC. "Involvement of connexin hemichanels in muscle atrophy induced by cachexia".
- 2009-present. Paloma Hacha, Doctor Ciencias Fisiológicas, PUC. Involvement of hemichannels in the degranulation response induced by immunoglobulin E in murine mast
cells”
- 2012-2014. Carolina Urrutia, (co-mentor with DR. Victoria Velarde). Master in Science: Free Radicals and Biomedicine”. The University of Vaparaíso. "Activation of
hemichannels
formed by connexins promotes Ca2+ influx, oxidative stress, and cell death, which is prevented by antioxidant agents that block hemichannels”.
- 2009-2013. Maximiliano Rovegno. (Co-mentor with Dra. Rommy von Benhardi). Doctorado en Ciencias Médicas, PUC. “Activation of inflammation in astrocytes induced by
ATP release via connexin hemichannels in an in vitro model of brain trauma".
- 2009-2013. Raúl Lagos Cabré. (Co-mentor with Dr. Ricardo Moreno). Doctorado en Ciencias Fisiológicas, PUCC. “Modulation of the ADAM protein family by xenostrogens
induces apoptosis in male germinal cells”.
- 2009-presente. Daniela Salas. (Co-mentor with Dr. Sergio Lavandero). Doctorado en Bioquímica, Universidad de Chile. “Regulation of Cx43 hemichannels by metabolic
stress”.
- 2008-2012. Pablo J. Sáez. Candidato a Doctor Ciencias Fisiológicas, PUC. Regulation and funtion of hemichannels and gap junction channels at the interaction between
dendritic cell and T lymphocytes.
- 2009-2012. Vania Figueroa. (Co-mentor with Dr. Agustín Martínez). Doctorado en Neurociencias, Universidad de Valparaíso. Activation of hemichannels formed by hCx26
and hPanx1 under physiologic and pathologic conditions and its possible role in the etiopatognesis of defness.
- 2008-2012. Luis A. Cea. Doctor Ciencias Fisiológicas, PUC. The activity controls the levels of connexins 39, 43 and 45 in rat skeletal muscles.
- 2008-2011. Kenji Shoji. Doctor Ciencias Fisiológicas, PUC. T-lymphocytes present Panx1 channels coupled to P2X7 receptor that mediate ATP-induced cell death induced
by extracellular ATP.
- 2008-presente. Romina Hernández (Co-tutor con Dra. M.V. Velarde). Candidato a Doctor Ciencias Fisiologicas, PUC. Effect of boldine in diabetic nephropaty in rats.
- 2008-2010. Juan A. Orellana. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role and regulation of astroglial channels formed by connexin43 in
neuronal death induced by hypoxia in high glucose: potentiation by neurodegenerative agents.
- 2005-2010. Manuel A. Riquelme. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role of panneixn1 hemichannels and extracellular ATP in myogenic
commitment and potentiation of muscle contraction in adult muscles.
- 2003-2008. Carolina Gatica de la Puente. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Role of hemichannels and gap junction channels in neuronal
differentiation and myogenic commitment acquisition in cell lines.
- 2005-2008. Helmuth Sánchez. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Involvement of connexin32 based channels in cell responses of two in
vitro models of ischemia-reperfusion.
- 2007-2008. Kurt A. Schalper. Doctor en Ciencias Médicas, PUC. Modulation of connexin hemichannel functional state by different physicochemical stimuli.
- 2003-2005. Mauricio Retamal. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas. PUC. Hemichannels formed by connexin43 are sensitive to redox changes.
- 2003-2005. Mauricio Garré. (Co-Director con la Dr. Veronica Abudara). Maestría en Biología, opción Neurociencias. PEDECIBA, Uruguay.Effects of fibroblast growth factor
FGF-1 on the intercellular communication medoated by gap junction channels on the neurotoxic phenotype of spinal astrocytes
- 2001-2005. (Co-Director con el Dr. M. Boric) Loreto P. Véliz. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Expression and function of gap junctions
between leukocytes and endothelial cells during inflammation.
- 1999-2003. Sra. Liliana Corvalán. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Gap junctions in contacts dendrict cell-T cell contacts”.
- 1999-2003. Dr. Jorge Contreras. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Biophysical properties and mechanism of action of hemichannels
formed by connexin43: possible participation in cell death induced by ischemia”.
- 1999-2004. Roberto Araya. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. Papel de los receptores purinérgicos y de las uniones en hendidura en el
proceso de diferenciación del músculo esquelético.
- 1998-2001. Dr. Eliseo A. Eugenín. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas, PUC. “Uniones en hendidura (UH) entre macrófagos: identificación de
conexinas, regulación de su expresión y su función en procesos metabólicos y celulares”.
- 1997-2000. Dr. María C. Brañes. Doctor en Ciencias Biológicas, mención, Biología Celular y Molecular. PUC. “Polymorphonuclear cells and endothelial cells form homo end
heterocellular gap junctions regulated by proinflammatory agents”.
- 1996-1998. Dr. Hernán González. Doctor en Ciencias Médicas. PUC. “Effect of endotoxemia on hepatic gap junctions”.
- 1996-1999. Dr. Agustín Martínez. Doctor en Ciencias Biológicas, mención Ciencias Fisiológicas. PUC. “In vitro regulation of glial gap junctions by inflammatory conditions”.
- 1989-1992. Dr. Viviana M. Berthoud, Department of Neuroscience, Albert Einstein College of Medicine, Nueva York, E.E.U.U. Ph.D. in Sciences. “Regulation of Gap
Junctions”.

24 Undergradute thesis
- 20178-2019. Catalina A. Ascencio Barría. “Análisis de la expresión y estado funcional de conexinas en cáncer de próstata”. (Cotutoría con Dr. Alejandro Godoy).
- 2017. Camila Aravena. Biochemist, PUC Role of glial hemichannels in epilepsy.
- 2015-2016. Fijiko Saveedra. “Participación de los hemicanales formados por conexinas en la atrofia
de los músculos esqueléticos rápidos adultos producida por denervación”. Bioquímico. Pontificia
Universidad Católica de Valparaiso.
- 2013-2014 Angelica Benvenuto.Biochemist, PUC. "Characterization of hemichannels and gap junction channels formed by connexina43 y pannexin 1 en adipocytes"
- 2011. (Co-mentor with Dr. Xavier Figueroa) Mauricio A. Lillo. Biochemist, PUC. Involvement hemichannels formed by connexins or pannexins in NO transport cross the
cell membrane in the vascular wall.
- 2009. Anibal Vargas. Lic. Biología, PUC. Characterization of antimycin A as an inhibitor of hemichannels formed by connexins.
- 2007-2009. (Co-mentor with Dr. Sergio Lavandero). Daniela P. Salas. Biochemist, Universidad de Chile. Hemichannels formed by connexins in volume regulation in
myocardiocytes exposed to hypoosmotic stress.
- 2009. Paola Soto. Biologist, PUC. Study on channels formed by connexin32 in a cell line derived from oligodendrocytes.
- 2008. Marlene Arismendi, Industrial Chemist, UTEM, Purification and biological activity of compounds derived from Boldo on channels formed by connexins.
- 2008. Diego Hernández, Biologist, PUC. Regulation of astroglial and neuronal channels formed by connexins in neurodegenerative processes.
- 2009. Natalia Vega, Biochemist, PUC. Regulation of cell membrane permeability by phosphorylation of hemichannels formed by connexin43 via protein kinase C (PKC).
- 2008. Paloma Harcha, Biologist, PUC. D2SC1 cells express functional hemichannels formed by pannexina1.
- 2006. Ariel Orellana. Biologist, PUC. The acquisition of myogenic commitment of reserve C2C12 cells requires activation of P2X receptors.
- 2006. Pablo J. Sáez, Biologist, PUC. ATP and TNF-? induce formation gap junction channels in microglia.
- 2006. Kenji Shoji, Biologist, PUC. The redox sensor of hemichannels formed by connexin43 is located in the carboxyl terminus of the protein subunit.
- 2005. Juan Andrés Orellana, Biologist, PUC. Chlorpromazine inhibits intercellular communication mediated by gap junctions in Gn-11 cells and astrocytes.
- 2005. Constanza J. Cortés, Biologist, PUC. Opening of Cx43 hemichannels in mediated by oxidative mechanisms in astrocytes”.
- 2005. Nicolás Palacios, Biologist, PUC. Modulation of the functional state of connexin-based channels by sexual hormones and tamoxifen in a cells line derived from human
breast cancer.
- 2002-2003. Manuel Riquelme-Biochemist, PUC. P2X receptors and connexin-based channels are needed for acquisition of myogenic commitment.
- 1998-1999. Patricio Orio-Biochemist, Universidad de Chile. Molecular characterization and functional gap junctions Express by Peripherals human lymphocytes.
- 1997. Alejandro Sepúlveda-Biochemist, Univ. Austral de Chile. Gap junctions between antigen-presenting cells and T cells: characterization and regulation of connexins.
- 1997. Eliseo Eugenín-Biochemist, Univ. Austral de Chile. Regulation of glycogen stores by norepinephrine in rat pineal gland: role of gap junctions.
- 1994-1995. Francisco Scheihing- Biochemist, Universidad Católica de Valparaíso. Effect of leptocarpin on gap junctions of epithelial cell lines.
- 1996. Erwin Strahsburger-Biochemist, Univ. Austral de Chile. Regulation of gap junctional Communications in astroglial cells of the pineal gland.


Difusión y Transferencia


3 workshops and three meeting symposia.


Premios y Distinciones

  •   Scopus

    Elsevier Editorial

    Chile, 2008

    Highest productivity and impact among Chilean Scientist in Pharmacology, Biochemistry, Cell Biology and Molecular Biology

  •   Member of the National Academy of Science USA

    UNIVERSIDAD DE VALPARAISO

    Estados Unidos, 2019

    Member of the National Academy of Science USA

  •   Member of the Latin American Academy of Science

    UNIVERSIDAD DE VALPARAISO

    Chile, 2017

    Cell Biology and Molecular Biology


 

Article (134)

Active acetylcholine receptors prevent the atrophy of skeletal muscles and favor reinnervation
Blockade of Hemichannels Normalizes the Differentiation Fate of Myoblasts and Features of Skeletal Muscles from Dysferlin-Deficient Mice
Connexin 43 deletion in astrocytes promotes CNS remyelination by modulating local inflammation
Glial connexins and pannexins in the healthy and diseased brain
Interferon-γ and high glucose-induced opening of Cx43 hemichannels causes endothelial cell dysfunction and damage.
Myofibers deficient in connexins 43 and 45 expression protect mice from skeletal muscle and systemic dysfunction promoted by a dysferlin mutation
Permeation of Molecules through Astroglial Connexin 43 Hemichannels Is Modulated by Cytokines with Parameters Depending on the Permeant Species
The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
Vitamin E Blocks Connexin Hemichannels and Prevents Deleterious Effects of Glucocorticoid Treatment on Skeletal Muscles
De novo expression of functional connexins 43 and 45 hemichannels increases sarcolemmal permeability of skeletal myofibers during endotoxemia
Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland
Pannexin-1 Channels Are Essential for Mast Cell Degranulation Triggered During Type I Hypersensitivity Reactions
Pannexin1 hemichannels are critical for HIV infection of human primary CD4+ T lymphocytes
Role of a RhoA/ROCK-Dependent Pathway on Renal Connexin43 Regulation in the Angiotensin II-Induced Renal Damage
Role of calcium permeable channels in dendritic cell migration
Angiotensin II-Induced Mesangial Cell Damage Is Preceded by Cell Membrane Permeabilization Due to Upregulation of Non-Selective Channels
Astroglial Ca 2+ -dependent hyperexcitability requires p2y 1 purinergic receptors and pannexin-1 channel activation in a chronic model of epilepsy
Connexin 43 Hemichannel Activity Promoted by Pro-Inflammatory Cytokines and High Glucose Alters Endothelial Cell Function
Role of calcium permeable channels in dendritic cell migration
SEPSIS-INDUCED CHANNELOPATHY IN SKELETAL MUSCLES IS ASSOCIATED WITH EXPRESSION OF NON-SELECTIVE CHANNELS
Trypanosoma cruzi Infection Induces Pannexin-1 Channel Opening in Cardiac Myocytes
ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors
ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X(7) receptors
Cannabinoids Prevent the Amyloid beta-Induced Activation of Astroglial Hemichannels: A Neuroprotective Mechanism
Connexins and pannexins in Alzheimer's disease.
High Glucocorticoid Levels During Gestation Activate the Inflammasome in Hippocampal Oligodendrocytes of the Offspring
Inhibition of glial hemichannels by boldine treatment reduces neuronal suffering in a murine model of Alzheimer's disease
On Biophysical Properties and Sensitivity to Gap Junction Blockers of Connexin 39 Hemichannels Expressed in HeLa Cells
Regulation of Connexin-Based Channels by Fatty Acids
A soluble activin receptor IIB fails to prevent muscle atrophy in a mouse model of spinal cord injury
Connexin hemichannels explain the ionic imbalance and lead to atrophy in denervated skeletal muscles
Connexin Hemichannels=> Methods for Dye Uptake and Leakage.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based hemichannels.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based hemichannels.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based hemichannels.
Fast skeletal myofibers of mdx mouse, model of Duchenne muscular dystrophy, express connexin hemichannels that lead to apoptosis
Linoleic acid permeabilizes gastric epithelial cells by increasing connexin 43 levels in the cell membrane via a GPR40- and Akt-dependent mechanism
The absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes
Connexin43 Hemichannels Mediate Secondary Cellular Damage Spread from the Trauma Zone to Distal Zones in Astrocyte Monolayers
Hemichannels Are Required for Amyloid beta-Peptide-Induced Degranulation and Are Activated in Brain Mast Cells of APPswe/PS1dE9 Mice
Keratitis-Ichthyosis-Deafness Syndrome-Associated Cx26 Mutants Produce Nonfunctional Gap Junctions but Hyperactive Hemichannels When Co-Expressed With Wild Type Cx43
Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells
Restraint stress increases hemichannel activity in hippocampal glial cells and neurons
Role of Akt and Ca2+ on cell permeabilization via connexin43 hemichannels induced by metabolic inhibition
Characterization of a Novel Water Pocket Inside the Human Cx26 Hemichannel Structure
Extracellular gentamicin reduces the activity of con nexin hemichannels and interferes with purinergic Ca2+ signaling in HeLa cells
Extracellular gentamicin reduces the activity of connexin hemichannels and interferes with purinergic Ca(2+) signaling in HeLa cells.
Gap Junction Intercellular Communication Mediated by Connexin43 in Astrocytes Is Essential for Their Resistance to Oxidative Stress
Opening of pannexin- and con nexin-based channels increases the excitability of nodose ganglion sensory neurons
Pannexin 1 channels in skeletal muscles
Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin cells.
Pannexin 1 regulates bidirectional hippocampal synaptic plasticity in adult mice
Pannexin1 channels act downstream of P2X(7) receptors in ATP-induced murine T-cell death
ATP is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro
Boldine Prevents Renal Alterations in Diabetic Rats
Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia
De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy
Diffusion of nitric oxide across cell membranes of the vascular wall requires specific connexin-based channels
Disruption in Connexin-Based Communication Is Associated with Intracellular Ca2+ Signal Alterations in Astrocytes from Niemann-Pick Type C Mice
FasL-triggered death of Jurkat cells requires caspase 8-induced, ATP-dependent cross-talk between Fas and the purinergic receptor P2X(7)
HIV increases the release of dickkopf-1 protein from human astrocytes by a Cx43 hemichannel-dependent mechanism.
Linoleic acid induces opening of connexin26 hemichannels through a PI3K/Akt/Ca2+-dependent pathway
Pannexin1 hemichannels are critical for HIV infection of human primary CD4(+) T lymphocytes
The ATP required for potentiation of skeletal muscle contraction is released via pannexin hemichannels
Connexin 36 is Expressed in Beta and Connexins 26 and 32 in Acinar Cells at the End of the Secondary Transition of Mouse Pancreatic Development and Increase During Fetal and Perinatal Life
Connexin and pannexin hemichannels in inflammatory responses of glia and neurons.
Connexin-based channels in astrocytes: How to study their properties
Critical role of the first transmembrane domain of Cx26 in regulating oligomerization and function
Glial hemichannels and their involvement in aging and neurodegenerative diseases
Glucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels
Permeation of Calcium through Purified Connexin 26 Hemichannels
Regulation of Intercellular Calcium Signaling Through Calcium Interactions with Connexin-Based Channels
Release of gliotransmitters through astroglial connexin 43 hemichannels is necessary for fear memory consolidation in the basolateral amygdala
Amyloid beta-Induced Death in Neurons Involves Glial and Neuronal Hemichannels
ATP and glutamate released via astroglial connexin 43 hemichannels mediate neuronal death through activation of pannexin 1 hemichannels
Boldina disminuye la apoptosis miocárdica post isquemia reperfusión en la rata
Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species
Hemichannels in the Neurovascular Unit and White Matter Under Normal and Inflamed Conditions
Cell membrane permeabilization via connexin-hemichannels in living and dying cells.
Connexin 43 hemichannels mediate the Ca2+ influx induced by extracellular alkalinization
Connexin43 is a pH-gated plasma membrane calcium channel.
FGF-1 induces ATP release from spinal astrocytes in culture and opens pannexin and connexin hemichannels
Hypoxia in High Glucose Followed by Reoxygenation in Normal Glucose Reduces the Viability of Cortical Astrocytes Through Increased Permeability of Connexin 43 Hemichannels
Inhibition of Cx43 hemichannels activated by pro-inflammatory treatment exhibited a neuroprotective effect against NMDA excitotocixity.
Inhibition of cytokine-induced connexin43 hemichannel activity in astrocytes is neuroprotective
ATP Released by Electrical Stimuli Elicits Calcium Transients and Gene Expression in Skeletal Muscle
Cannabinoids prevent the opposite regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory treatments
Dysfunctions of the Diffusional Membrane Pathways Mediated Hemichannels in Inherited and Acquired Human Diseases
Gap Junctions at the Dendritic Cell-T Cell Interface Are Key Elements for Antigen-Dependent T Cell Activation
Metabolic inhibition increases activity of connexin-32 hemichannels permeable to Ca2+ in transfected HeLa cells
Modulation of brain hemichannels and gap junction channels by pro-inflammatory agents and their possible role in neuro-egeneration.
Astrocytes as connexin-dependent signaling cells for local blood flow regulation.
Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+](i) responses
Currently used methods for identification and characterization of hemichannels
Functional role of gap junctions in cytokine-induced leukocyte adhesion to endothelium in vivo
The connexin hemichannel composition determines the FGF-1-induced membrane permeability and free Ca2+i responses.
Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinflammatory cytokines released from activated microglia
Functional gap junctions facilitate melanoma antigen transfer and cross-presentation between human dendritic cells
Inflammatory conditions induce gap junctional communication between rat Kupffer cells both in vivo and in vitro
Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse dendritic cells
Opening of connexin 43 hemichannels is increased by lowering intracellular redox potential
Chlorpromazine reduces the intercellular communication via gap junctions in mammalian cells
S-nitrosylation and permeation through connexin 43 hemichannels in astrocytes: Induction by oxidant stress and reversal by reducing agents
Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43
HISTAMINE REDUCES GAP JUNCTIONAL COMMUNICATION OF HUMAN TONSIL HIGH ENDOTHELIAL CELLS IN CULTURE
Role of connexin-based gap junction channels and hemichannels in ischemia-induced cell death in nervous tissue
The formation of skeletal muscle myotubes requires functional membrane receptors activated by extracellular ATP
Connexin43 is expressed in mouse fetal ovary
Functioning of Cx43 hemichannels demonstrated by single channel properties
Gating and regulation of connexin 43 (Cx43) hemichannels
Presence and importance of connexin43 during myogenesis
TNF-? plus IFN-? induce connexin43 expression and formation of gap junctions between human monocytes/macrophages that enhance physiological responses
ACTIVATION OF HUMAN POLYMORPHONUCLEAR CELLS INDUCES FORMATION OF FUNCTIONAL GAP JUNCTIONS AND EXPRESSION OF CONNEXINS
Identification of second messengers that induce expression of functional gap junctions in microglia cultured from newborn rats
Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture
Regulation of hepatic connexins in cholestasis: possible involvement of Kupffer cells and inflammatory mediators
Expression of connexin43 in mouse Leydig, Sertoli, and germinal cells at different stages of postnatal development
Microglia at brain stab wounds express connexin 43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha
Gap junctions in cells of the immune system: structure, regulation and possible functional roles
Gap junctions in the chicken pineal gland
Regulation of astrocyte gap junctions by hypoxia-reoxygenation
Arachidonic acid-induced dye uncoupling in rat cortical astrocytes is mediated by arachidonic acid byproducts
Cells of the carotid body express connexin43 which is up-regulated by cAMP
Chapter 25: Gap Junctions in Inflammatory Responses: Connexins, Regulation and Possible Functional Roles
The ependymocytes of the bovine subcommissural organ are functionally coupled through gap junctions
Gap junctional communication coordinates vasopressin-induced glycogenolysis in rat hepatocytes
Identification of gap junctions in the oviduct and regulation of connexins during development and by sexual hormones
Phosphorylation of connexin43 and the regulation of neonatal rat cardiac myocyte gap junctions
REGULATION OF GAP-JUNCTIONS BY PROTEIN PHOSPHORYLATION
Regulation of glycogen content in rat pineal gland by norepinephrine
C-erbB2/neu transfection induces gap junctional communication incompetence in glial cells
Regulated assembly of connexin33 and connexin43 into rat Sertoli cell gap junctions
Biological mechanisms involved in the spread of traumatic brain damage.
Sepsis-Induced Channelopathy in Skeletal Muscles is Associated with Expression of Non-Selective Channels.

Abstract (2)

HIV infection of astrocytes increases release of Dickkopf-1 protein by a gap junction and hemichannel dependent mechanism
Specific ablation of connexin 43 delays skeletal muscle regeneration in vivo

BookWhole (1)

Connexin and Pannexin Based Channels in the Nervous System: Gap Junctions and More

ConferencePaper (1)

Brain Research Reviews: Preface

EditorialMaterial (2)

We've had important advances in the connexin/pannexin field, yet there is still much to do
Astrocytes as connexin-dependent signaling cells for local blood flow regulation

Metodologia (1)

UNIONES EN HENDIDURA Y SU PAPEL FUNCIONAL EN EL TRACTO REPRODUCTOR FEMENINO

Proyecto (25)

DIVERSE CELL TYPES PRESENT PANNEXIN1-BASED GAP JUNCTION CHANNELS WITH DISTINCT REGULATORY, FUNCTIONAL AND PHARMACOLOGICAL PROPERTIES
Centro Interdiciplianrio de Neurociencias de Valparaiso
UNCOVERING THE ROLE OF HEMICHANNELS IN GLIAL AND NEURONAL DYSFUNCTION INDUCED BY ALPHA-SYNUCLEIN
Neuronal derived factor(s) and mechanim(s) that repress the expression of non-selective ion channels in normal adult skeletal muscles
RELEVANCIA DEL ESTRÉS OXIDATIVO, LA DISFUNCIÓN MITOCONDRIAL Y LA EXPRESIÓN DE CONEXINAS EN LA INDUCCIÓN DE ATROFIA MUSCULAR POR DEXAMETASONA
CONNEXIN HEMICHANNELS AS MEDIATORS OF SARCOLEMMAL AND MITOCHONDRIAL DYSFUNCTION IN RESPIRATORY AND PERIPHERAL MUSCLE IN THE PATHOGENESIS OF SEPSIS ASSOCIATED-INTENSIVE CARE UNIT ACQUIRED WEAKNESS (ICUAW)
"INVOLVEMENT OF HEMICHANNELS IN GLIA PRE-ACTIVATION BY AUDITORY STRESS TO INFLAMMATION PROMOTED BY POLY (I=>C) AND CONSEQUENCES ON NEURONAL ARBORIZATION AND SURVIVAL"
REGULACION DEL TRANSPORTE DE GLUCOSA, A TRAVES DE SUS TRANSPORTADORES ESPECIFICOS Y HEMICANALES, POR ACIDOS GRASOS ESENCIALES EN INTESTINO DELGADO
Centro Interdiciplianrio de Neurociencias de Valparaiso
INVOLVEMENT OF TACE/ADAM17 AND PANNEXIN HEMICHANNELS IN XENOESTROGENS INDUCED APOPTOSIS IN MAMMALIAN SPERMATOGENESIS
REGULATION OF CONNEXIN43 HEMICHANNELS PROPERTIES BY PKC, PKA AND MAP KINASES.
ROLE AND REGULATION OF HEMICHANNELS IN FUNCTIONAL RESPONSES OF INACTIVE, ACTIVE AND ACUTELY CACHEXIC ADULT SKELETAL MUSCLES.
Role nad regulation of hemichannels in functional responses of inactive, active and acutly cachexic adult skeletal muscle
COORDINATION OF MICROVASCULAR FUNCTION BY LONG DISTANCE ENDOTHELIAL CELL SIGNALING BASED ON CONNEXIN-MEDIATED COMMUNICATION AND VOLTAGE-DEPENDENT VASODILATOR MECHANISMS
PATHOGENIC MECHANISM OF CONNEXIN26 MUTATIONS INVOLVED IN SYNDROMIC AND NON-SYNDROMIC DEAFNESS
PAPEL Y REGULACION DE LOS CANALES FORMADOS POR CONEXINA43 ASTROGLIAL EN LA DISFUNCION Y MUERTE CELULAR EN TEJIDO CEREBRAL IN VITRO INDUCIDA POR ISQUEMIA-REPERFUSION EN ALTA GLUCOSA.
ESTUDIO DEL PAPEL REGULADOR DE LAS CONEXINAS SOBRE EL PROCESO DE NEUROGENESIS Y GLIOGENESIS IN VITRO
IDENTIFICACION Y REGULACION DE VIAS DE SENALIZACION INTERCELULAR EN EL EPITELIO DEL OVIDUCTO QUE CONTROLAN EL TRANSPORTE MUCOCILIAR EN CONDICIONES FISIOLOGICAS Y FISIPATOLOGICAS.
CARACTERIZACION DE MECANISMOS MODULADORES DEL ESTADO FUNCIONAL DE HEMICANALES DE LAS UNIONES EN HENDIDURA EN CELULAS DEL SISTEMA NERVIOSO CENTRAL.
IMPORTANCIA Y REGULACION DE MECANISMOS DE SENALIZACION INTERCELULAR EN LA RESPUESTA INFLAMATORIA
REGULACION Y FUNCION DE LAS UNIONES EN HENDIDURA EN MODELOS DE INFLAMACION "IN VITRO"
MECANISMOS DE COORDINACION Y CONTROL DE LA ACTIVIDAD CILIAR EN EL EPITELIO DEL OVIDUCTO.
REGULACION DE LAS UNIONES COMUNICANTES DEL ACINO HEPATICO Y SU POSIBLE FUNCION EN LA RESPUESTA GLUCOGENICA DEL HIGADO
REGULACION Y FUNCION DE LAS UNIONES COMUNICANTES DE LA GLANDULA PINEAL
SUPEROXIDOS Y PATOGENIA DEL SINDROME DE SHOCK.

Review (25)

Connexins and pannexins in Alzheimer's disease
Direct Intercellular Communications and Cancer: A Snapshot of the Biological Roles of Connexins in Prostate Cancer
Innexins: Expression, Regulation, and Functions
Role of astrocyte connexin hemichannels in cortical spreading depression
Connexin-Dependent Neuroglial Networking as a New Therapeutic Target
Connexins and Pannexins in Bone and Skeletal Muscle
Regulation of pannexin and connexin channels and their functional role in skeletal muscles
Hunting for connexin hemichannels
Neuronal involvement in muscular atrophy
Regulation of Hemichannels and Gap Junction Channels by Cytokines in Antigen-Presenting Cells
Connexin and pannexin hernichannels in brain glial cells: properties, pharmacology, and roles
Possible Involvement of TLRs and Hemichannels in Stress-Induced CNS Dysfunction via Mastocytes, and Glia Activation
Role of Gap Junctions and Hemichannels in Parasitic Infections
Biological mechanisms involved in the spread of traumatic brain damage
Connexin and pannexin hemichannels in inflammatory responses of glia and neurons
Connexin- and Pannexin-Based Channels in Normal Skeletal Muscles and Their Possible Role in Muscle Atrophy
Glial connexin expression and function in the context of Alzheimer's disease
Modulation of gap junction channels and hemichannels by growth factors
The Role of Gap Junction Channels During Physiologic and Pathologic Conditions of the Human Central Nervous System
Cell membrane permeabilization via connexin hemichannels in living and dying cells
Modulation of Brain Hemichannels and Gap Junction Channels by Pro-Inflammatory Agents and Their Possible Role in Neurodegeneration
Possible involvement of different connexin43 domains in plasma membrane permeabilization induced by ischemia-reperfusion
Connexin-based gap junction hemichannels: Gating mechanisms
GAP JUNCTION HEMICHANNELS IN ASTROCYTES OF THE CNS
New roles for astrocytes: Gap junction hemichannels have something to communicate
181
Juan Saez

Professor

Instituto de Neurociencias

Universidad de Valparaiso

Valparaíso, Chile

35
Juan Orellana

Associate Professor

Departamento de Neurología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

29
Juan Sáez

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

20
Mauricio Retamal

Associate Professor

Centro de Fisiología Celular e Integrativa

Universidad del Desarrollo

Santiago, Chile

9
Luis Cea

Profesor programa teaching

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

8
Carlos Puebla

Profesor Asistente

Universidad de O'Higgins

Rancagua, Chile

7
Carlos Puebla

Assistant Professor

Universidad de O'Higgins

Santiago, Chile

6
vania figueroa

Coordinadora de Vinculación de Investigación Aplicada

Centro de Comunicación de las Ciencias

Universidad Autónoma de Chile

Santiago, Chile

6
Jose Luis Vega

Profesor Asociado

Biomedico

Universidad de Antofagasta

Antofagasta, Chile

5
Xavier Figueroa

Associate Professor

Departamento de Fisiologia

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

5
ROSALBA ESCAMILLA

Investigador asociado

Fisiología

Pontificia Universidad Católica de Chile

Santiago, Chile

5
Agustin Martinez

Profesor Titular

Instituto de Neurociencia

UNIVERSIDAD DE VALPARAÍSO, CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAÍSO

Valparaíso, Chile

4
Cristian Vilos

Profesor Jornada Completa

Medicina

Universidad de Talca

Talca, Chile

3
HELMUTH SANCHEZ

INVESTIGADOR ADJUNTO

Laboratorio de Conexinas y Panexinas

CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAISO

VALPARAISO, Chile

3
Aníbal Vargas

Investigador postdoctoral

Universidad de O'Higgins

Rancagua, Chile

3
Claudia Saez

Associate Professor

Hematology-Oncology

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Iván Barría

Investigador Post doctoral

Centro Interdisciplinario de Neurociencia de Valparaíso

Valparaíso, Chile

3
Rommy von Bernhardi

Full Professor

Neurology

DEPT. NEUROLOGY, FAC. MEDICINE PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

3
Tomas Perez-Acle

Full Professor

Computational Biology Lab

Fundación Ciencia para la Vida

Santiago, Chile

2
Jimmy Stehberg

Associate Professor

Biological Sciences

UNIVERSIDAD ANDRÉS BELLO

SANTIAGO, Chile

2
Carlos Lagos

Profesor Asociado

FACULTAD DE MEDICINA Y CIENCIA

UNIVERSIDAD SAN SEBASTIÁN

SANTIAGO, Chile

2
Rodrigo Acuña

Profesor Asistente Investigador

Medicina

Universidad del Desarrollo

Santiago, Chile

2
Alex Vielma

Postdoctoral researcher

Centro Interdisciplinario de Neurociencias de Valparaíso

Valparaiso, Chile

2
Alexis Kalergis

Full Professor

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE. FACULTAD DE CIENCIAS BIOLÓGICAS

Santiago, Chile

2
Loreto Véliz

Profesor Asistente Adjunto

Fisiología

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Mariana Casas

Assistant Professor

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Hernan Gonzalez Diaz

Associate Professor

Cirugía Oncológica

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Mario Rosemblatt

Executive Director

-

Fundacion Ciencia & Vida

Santiago, Chile

1
Jorge Bevilacqua

Profesor Titular

Neurología y Neurocirugía

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, ESCUELA DE MEDICINA

Santiago, Chile

1
Dinka Mandakovic

Profesora Asistente

Centro GEMA

Universidad Mayor de Chile

Santiago, Chile

1
Alberto Martin

Assistant professor

Centro de Genómica y Bioinformática

Universidad Mayor

Santiago, Chile

1
Sonja Buvinic

Associate Professor

Institute for Research in Dental Sciences

Universidad de Chile

Santiago, Chile

1
ANDRES KLEIN

Assistant Professor/Director (Head) Center for Genetics and Genomics; Director, PhD Program in Sciences and Innovation in Medicine

Centro de Genética y Genómica

Universidad del Desarrollo-Clínica Alemana

SANTIAGO, Chile

1
Jaime De Calisto

Director Docente

Escuela de Biotecnología

Universidad Mayor

Santiago, Chile

1
Oliver Schmachtenberg

Investigador titular

Instituto de Biología

Universidad de Valparaíso, CINV

Valparaíso, Chile

1
Maria Bono

Profesor titular

Biologia

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Maria Ocaranza

Asociate Professor

Cardiovascular Diseases Division

Pontificia Universidad Catolica de Chile

Santiago, Chile

1
Alejandra Vielma

Post-doctorado

Ciencias Biológicas, Ciencias Fisiológicas

Pontificia Universidad Católica de Chile

Santiago, Chile

1
ISAAC GARCÍA

Profesor Adjunto

Instituto de Neurociencias

Universidad de Valparaiso

Valparaiso, Chile

1
Alvaro Ardiles

Assistant Professor

Medicine

Universidad de Valparaíso

Valparaiso, Chile

1
Pablo Caviedes

Full professor

ICBM

PROGRAMA DE FARMACOLOGÍA MOLECULAR Y CLÍNICA, ICBM, FAC. DE MEDICINA, U. DE CHILE

Santiago, Chile

1
Fernando Delgado

Associate Professor

Division of Medicine

Universidad Catolica del Maule

Talca, Chile

1
Sergio Lavandero

Director

Advanced Center for Chronic Diseases (ACCDiS)

UNIVERSIDAD DE CHILE, FAC CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

1
Pablo Olivero

Profesor Adjunto

Patologia y Fisiologia

Universidad de Valparaiso

Viña del Mar, Chile

1
Luis Leon

Director of Bioinformatics

R&D

Genoma Mayor SpA

Santiago, Chile

1
Julio Alcayaga

Profesor Titular

Biología

UNIVERSIDAD DE CHILE, FAC. CIENCIAS, DEPTO. BIOLOGÍA

Santiago, Chile

1
María García

Profesor Titular

Biología Celular

UNIVERSIDAD DE CONCEPCIÓN

Concepción, Chile

1
Felipe Simon

Profesor Titular

Department of Biological Sciences

UNIVERSIDAD ANDRES BELLO FAC DE CS BIOLOGICAS, DPTO DE CS BIOLOGICAS

Santiago, Chile

1
PEDRO ZAMORANO

Profesor Asociado

Depto Biomédico

Universidad de Antofagasta

Antofagasta, Chile

1
Ricardo Fernández

Associate Professor

Health

Universidad de Los Lagos

Osorno, Chile

1
Mercedes López

Profesora Asociada

Instituto de Ciencias Biomédicas

Universidad de chile

Santiago, Chile

1
Adrian Palacios

Profesor

Instituto de Neurosciencia

UNIVERSIDAD DE VALPARAISO

Valparaiso, Chile

1
David Rovegno

PROFESOR ASISTENTE ADJUNTO

Medicina Intensiva

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Roberto Elizondo

Profesor Asistente

Departamento de Biología Celular

Universidad de Concepción

CONCEPCION, Chile

1
Marlene Arismendi

INVESTIGADOR JEFE

INVESTIGACION Y DESARROLLO

NANOTEC S.A

SANTIAGO, Chile

1
Gonzalo Gomez

Profesor Asistente

Instituto de Ciencias de la Saluda

Universidad Autónoma de Chile

Santiago, Chile

1
Jorge Gonzalez

Profesor Titular

Departamento de Tecnología Médica

UNIVERSIDAD DE ANTOFAGASTA

Antofagasta, Chile

1
Mauricio Henriquez

Profesor asistente

PROGRAMA DE FISIOLOGÍA Y BIOFíSICA

Universidad de Chile / Facultad de Medicina

Santiago, Chile

1
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

1
ramon latorre

Profesor

Instituto de Neurociencia

Universidad de Valparaíso

5, Chile

1
Alejandro Godoy

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

1
César Echeverría

DIRECTOR DE INVESTIGACIÓN

Medicina

Universidad de Atacama

COPIAPO, Chile

1
alejandro bernardin

PhD (c)

Fundación Ciencia y Vida

Santiago, Chile

1
Trinidad Montero

postdoctorate

Medicine

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Raul Araya

Docente Investigador

Facultad de Ingenieria y Tecnologia

Universidad San Sebastián

Santiago, Chile

1
DOMINGO BONANSCO

Profesor Titular

Instituto de Fisiologia

UNIVERSIDAD DE VALPARAISO, FACULTAD DE CIENCIAS

Valparaiso, Chile

1
Daniel Escobar

PROFESIONAL

SALUD AMBIENTAL

INSTITUTO DE SALUD PÚBLICA DE CHILE

SANTIAGO, Chile

1
Ricardo Ceriani

Postdoctorado

Farmacia

Universidad Valparaíso

Valparaíso, Chile

1
Marcela Hermoso

Full Professor

School of Medicine

UNIVERSIDAD DE CHILE

Santiago, Chile

1
FRANCISCO NUALART

Full Professor and Scientific Director

Cellular Biology and Center for Advanced Microscopy CMA BIO-BIO

UNIVERSIDAD DE CONCEPCION

Concepcion, Chile

1
JOSE GARATE

Profesor Asistente

Instituto de Neurociencia

Universidad de Valparaíso

Valaparaiso, Chile

1
Enrique Jaimovich

Full Professor

ICBM

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Claudia Pareja

Postdoc

Departamento de Hematología-Oncología

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Enrique Brandan

Profesor Titular

Biología Celular y Molecular

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Margarita Pizarro

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Erwin Strahsburger

Instructor Professor

Universidad Arturo Prat

Iquique, Chile