Man

Berta Alejandra Espinosa Escalona

Assistant Professor

Universidad de Chile

Santiago, Chile

Líneas de Investigación


Insulin resistance and oxidative stress Calcium signaling in skeletal muscle

Educación

  •  Ciencias Biomédicas, UNIVERSIDAD DE CHILE. Chile, 2008
  •  Ciencias Biológicas, mención bioquímica y biología molecular, UNIVERSIDAD DE CHILE. Chile, 2003
  •  Tecnólogo Médico, mención laboratorio clínico, hematología y banco de sangre, UNIVERSIDAD DE CHILE. Chile, 1997

Experiencia Académica

  •   Profesor asociado Full Time

    UNIVERSIDAD DE CHILE

    Medicina

    Santiago, Chile

    1999 - A la fecha

  •   Profesor titular Part Time

    UNIVERSIDAD DE VALPARAISO

    Campus San Felipe

    San Felipe, Chile

    2019 - A la fecha

Experiencia Profesional

  •   Assistant professor Full Time

    Universidad de Chile

    Santiago, Chile

    1999 - A la fecha

Formación de Capital Humano


2013 Cambios en la expresión de proteínas relacionadas con la homeostasis del calcio en músculo esquelético de ratones obesos
Tutores: Dra. Alejandra Espinosa
Tesis para optar al título de Tecnólogo Médico
Institución: Escuela de Tecnología Médica. Facultad de Medicina. Universidad de Chile
Autor: Cristián Campos Acevedo

2012. Medición de la translocación de GLUT4 e incorporacion de glucosa en fibras musculares esqueléticas de ratones sometidos a dieta alta en grasas
Tutores: Dra. Alejandra Espinosa /Dr. Ariel Contreras
Tesis para optar al título de Tecnólogo Médico
Institución: Escuela de Tecnología Médica. Facultad de Medicina. Universidad de Chile
Autor: Priscila Utreras Mendoza

2011 Estudio de la expresión de NADPH oxidasa en músculo esquelético de ratones con resistencia a la insulina
Tutores: Dra. Alejandra Espinosa y Dra. Nevenka Juretic
Tesis para optar al título de Tecnólogo Médico
Institución: Escuela de Tecnología Médica. Facultad de Medicina. Universidad de Chile
Autor: Claudia Contardo Flores.

2010. Glutationilación del receptor de Ryanodina en músculo esquelético de ratones resistentes a la insulina.
Tutores: Dra. Alejandra Espinosa y Dra. Gina Sánchez
Tesis para optar al título de Tecnólogo Médico
Institución: Escuela de Tecnología Médica. Facultad de Medicina. Universidad de Chile
Autor: Julio Miranda Betancourt.

2006. Translocación del complejo transcripcional six1/eya1 por estimulación eléctrica en células de músculo esquelético.
Tutores: Dr. Enrique Jaimovich y Dra. Alejandra Espinosa
Tesis para optar al título de Tecnólogo Médico
Institución: Escuela de Tecnología Médica. Facultad de Medicina. Universidad de Chile
Autor: Javiera León

2013 “El ATP extracelular incrementa la producción de ros vía NOX2, activa a Nrf2 e induce la expresión de enzimas antioxidantes en músculo esquelético”
Programa: Magíster en Ciencias Biológicas con mención en Fisiología
Institución: Escuela de Postgrado. Facultad de Medicina, Universidad de Chile
Autor: Alexis Díaz Vegas

2010 “Producción de especies reactivas de oxígeno en células fagocíticas de ratones resistentes a insulina”
Programa: Magíster en Ciencias Biológicas con mención en Inmunología
Institución: Escuela de Postgrado. Facultad de Medicina, Universidad de Chile
Autor: T.M. Alejandro López

2008. Factores de riesgo relacionados a la prevalencia de anemia ferropénica en mujeres gestantes a partir de la semana 13 del hospital gineco-obstetrico.
Programa: Programa de Maestría y Diploma Superior de Especialidad en Análisis Clínicos
Institución: Universidad Andina Simón Bolívar, Sucre, Bolivia
Autor: Javier Cuenca Sarabia


Premios y Distinciones

  •   Premio a la excelencia académica 2012

    UNIVERSIDAD DE CHILE

    Chile, 2013

    Premio entregado al investigador con la publicación de impacto más alto del año 2012 de su departamento


 

Article (41)

Composition and biological effects of goldenberry byproducts: an overview
Iron-induced derangement in hepatic ?-5 and ?-6 desaturation capacity and fatty acid profile leading to steatosis: Impact on extrahepatic tissues and prevention by antioxidant-rich extra virgin olive oil
Lipid droplets are both highly oxidized and Plin2-covered in hepatocytes of diet-induced obese mice
The metabolic dysfunction of white adipose tissue induced in mice by a high-fat diet is abrogated by co-administration of docosahexaenoic acid and hydroxytyrosol
Concentration of EPA and DHA from Refined Salmon Oil by Optimizing the Urea-Fatty Acid Adduction Reaction Conditions Using Response Surface Methodology
Hydroxytyrosol supplementation ameliorates the metabolic disturbances in white adipose tissue from mice fed a high-fat diet through recovery of transcription factors Nrf2, SREBP-1c, PPAR-gamma and NF-kappa B
Reduction of high-fat diet-induced liver proinflammatory state by eicosapentaenoic acid plus hydroxytyrosol supplementation: involvement of resolvins RvE1/2 and RvD1/2
Molecular mechanisms related to the hepatoprotective effects of antioxidant-rich extra virgin olive oil supplementation in rats subjected to short-term iron administration
Effects of rosa mosqueta oil supplementation in lipogenic markers associated with prevention of liver steatosis.
Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice.
Molecular adaptations underlying the beneficial effects of hydroxytyrosol in the pathogenic alterations induced by a high-fat diet in mouse liver=> PPAR-α and Nrf2 activation, and NF-κB down-regulation.
Muscle Lipid Metabolism=> Role of Lipid Droplets and Perilipins.
Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet
Extra virgin olive oil reduces liver oxidative stress and tissue depletion of long-chain polyunsaturated fatty acids produced by a high saturated fat diet in mice
NOX2 Inhibition Impairs Early Muscle Gene Expression Induced by a Single Exercise Bout.
NOX2 Inhibition Impairs Early Muscle Gene Expression Induced by a Single Exercise Bout.
Reactive oxygen species and calcium signals in skeletal muscle: A crosstalk involved in both normal signaling and disease.
Reactive oxygen species and calcium signals in skeletal muscle=> A crosstalk involved in both normal signaling and disease.
Vegetable oils rich in alpha linolenic acid increment hepatic n-3 LCPUFA, modulating the fatty acid metabolism and antioxidant response in rats.
Dietary Rosa mosqueta (Rosa rubiginosa) oil prevents high diet-induced hepatic steatosis in mice
Reduction in the desaturation capacity of the liver in mice subjected to high fat diet: Relation to LCPUFA depletion in liver and extrahepatic tissues
ROS Production via P2Y(1)-PKC-NOX2 Is Triggered by Extracellular ATP after Electrical Stimulation of Skeletal Muscle Cells
The cholesterol-lowering agent methyl-beta-cyclodextrin promotes glucose uptake via GLUT4 in adult muscle fibers and reduces insulin resistance in obese mice
Insulin elicits a ROS-activated and an IP3-dependent Ca2+ release, which both impinge on GLUT4 translocation
N-3 long-chain PUFA supplementation prevents high fat diet induced mouse liver steatosis and inflammation in relation to PPAR-alpha upregulation and NF-kappa B DNA binding abrogation
Reversal of high-fat diet-induced hepatic steatosis by n-3 LCPUFA: role of PPAR-alpha and SREBP-1c
Electrical Stimuli Release ATP to Increase GLUT4 Translocation and Glucose Uptake via PI3K gamma-Akt-AS160 in Skeletal Muscle Cells
Insulin-Dependent H2O2 Production Is Higher in Muscle Fibers of Mice Fed with a High-Fat Diet
Methyl-beta-cyclodextrin increases GLUT4-mediated glucose transport in skeletal muscle fibers from insulin resistant mice
Prevention of liver steatosis through fish oil supplementation: correlation of oxidative stress with insulin resistance and liver fatty acid content
Assessment of red blood cell glutathione status in insulin resistance
Bioconversion of alpha-linolenic acid to n-3 LCPUFA and expression of PPAR-alpha, acyl coenzyme A oxidase 1 and carnitine acyl transferase I are incremented after feeding rats with alpha-linolenic acid-rich oils
N-3 Long-Chain Polyunsaturated Fatty Acid Supplementation Significantly Reduces Liver Oxidative Stress in High Fat Induced Steatosis
NADPH Oxidase and Hydrogen Peroxide Mediate Insulin-induced Calcium Increase in Skeletal Muscle Cells
Evaluation of satisfaction with a self-learning module for urinary sediment analysis using a technological platform (e-aula) Evaluación de satisfacción del módulo de autoenseñanza para el estudio del sedimento urinario en una plataforma tecnológica (e-aula)
Myotube depolarization generates reactive oxygen species through NAD(P)H oxidase; ROS-elicited Ca 2+ stimulates ERK, CREB, early genes
Myotube depolarization generates reactive oxygen species through NAD(P)H oxidase; ROS-elicited Ca2+ stimulates ERK, CREB, early genes
Capacitative calcium entry in testosterone-induced intracellular calcium oscillations in myotubes
IGF-I and insulin induce different intracellular calcium signals in skeletal muscle cells
Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
Testosterone stimulates intracellular calcium release and mitogen-activated protein kinases via a G protein-coupled receptor in skeletal muscle cells

Abstract (10)

NOX2-Dependent ROS Production is Essential for the Response to Endurance Exercise in Skeletal Muscle.
ATP release induced by electrical stimuli increase ROS production and RyR glutathionylation via P2Y1-PKC-NOX2 in skeletal muscle fibers
Cholesterol accumulation in skeletal muscle: a potential novel targetable pathway in insulin resistance
Insulin Induces both H2O2 Production and IP3-Dependent Mitochondria Ca2+ Uptake. H2O2 Oxidizes RyR to Elicit Ca2+ Release and GLUT4 Translocation in Skeletal Muscle Cells
Cell cholesterol levels determine glucose uptake and insulin resistance in skeletal muscle
NAD(P)H oxidase-dpendent H2O2 production and RyR-IP3R activation are required for insulin induced GLUT4 translocation and glucose uptake in skeletal muscle cells
ATP release induced by electrical stimulation increases glucose uptake via AKT in skeletal muscle
H2O2 Production and Glutathionylation of Ryanodine Receptor in Skeletal Muscle from Insulin Resistance Mice
Increased H2O2 Production and NADPH Oxidase Levels in Monocytes from Insulin Resistance Mice
Rapid calcium signals linked to phosphorylation of MAP kinases are induced by androgens in skeletal muscle cells

ConferencePaper (1)

Bioconversion of ?-linolenic acid to n-3 LCPUFA and expression of PPAR-alpha, acyl coenzyme A oxidase 1 and carnitine acyl transferase i are incremented after feeding rats with ?-linolenic acid-rich oils

Proyecto (5)

Effect of refractance window technique drying conditions on general quality, health-related compounds, and energetic issues of thin layer drying process of physalis peruviana pulp
EXCITATION-METABOLISM COUPLING IN SKELETAL MUSCLE; BASIC MECHANISMS AND THEIR ALTERATION IN OBESITY AND AGING
Evaluacion de la actividad de nadph oxidasa-2 en celulas mononucleares perifericas de obesos y delgados y su potencial inhibicion por vanillina
Metabolic stress signaling in cancer, cardiovascular diseases and diabetes
ROLE OF CALCIUM AND H2O2 IN THE PATHOPHYSIOLOGY OF INSULIN RESISTANCE IN SKELETAL MUSCLE
44
Berta Espinosa

Assistant Professor

Tecnología Médica

Universidad de Chile

Santiago, Chile

22
Enrique Jaimovich

Full Professor

ICBM

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

10
Rodrigo Valenzuela

Profesor Asociado

Nutrición

Universidad de Chile

Santiago, Chile

9
César Osorio

Associate Professor

Kinesiología

Universidad Metropolitana de Ciencias de la Educación

Santiago, Chile

8
Paola Llanos

Profesor Asistente

Instituto de Investigacion en Ciencias Biomédicas

FACULTAD DE ODONTOLOGIA. UNIVERSIDAD DE CHILE

Santiago, Chile

6
Amanda D'Espessailles

Investigadora Postdoctoral

Instituto de Ciencias de a Salud

Universidad de O'Higgins

Rancagua, Chile

6
M. Cecilia Hidalgo

Professor

ICBM

Universidad de Chile

Santiago, Chile

5
Gladys Tapia

Academica

Farmacología Molecular y Clínica

FACULTAD DE MEDICINA UNIVERSIDAD DE CHILE

Santiago, Chile

4
Sergio Lavandero

Director

Advanced Center for Chronic Diseases (ACCDiS)

UNIVERSIDAD DE CHILE, FAC CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

4
Nalda Romero

Profesora Asociada

Ciencia de los Alimentos

UNIVERSIDAD DE CHILE

Santiago, Chile

4
Ariel Contreras

assistance professor

Institute for Research in Dental Science

UNIVERSIDAD DE CHILE

Santiago, Chile

3
Francisca Echeverría

Profesora Asistente

Nutrición

Universidad de Chile

Santiago, Chile

2
Alexis Diaz

PhD stundent

Fisiología

universidad de Chile

Santiago, Chile

2
Maria Hernandez

Académico. Profesor Asistente

Nutrición

Universidad de Chile

Santiago , Chile

2
JOSE GALGANI

ASSOCIATE PROFESSOR

NUTRITION, DIABETES AND METABOLISM; AND UDA-CIENCIAS DE LA SALUD, NUTRICION Y DIETETICA

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
Luis Puente

Academico

Ciencias de los Alimentos y Tecnologia Quimica

UNIVERSIDAD DE CHILE, FACULTAD DE CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile

2
Cristian Campos

Entrenamiento Post Doctorado

Fisiología

Universidad de Chile

Santiago, Chile

2
Carlos Valenzuela

INTA, UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Andres Bustamante

Académico

Nutrición

Universidad de Chile, Facultad de Medicina

Santiago, Chile

1
Francisco Pérez

DIRECTOR

UNIVERSIDAD DE CHILE

Santiago, Chile

1
Gina Leonelli

Director de Carrera

Ciencias Agropecuarias y Acuícolas

Universidad Católica de Temuco

Temuco, Chile

1
Mariana Casas

Assistant Professor

FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Pamela Romanque

Residente

Imagenología

Hospital Clínico Universidad de Chile

Santiago, Chile

1
Manuel Ruz

Professor and Chair

Nutrition

DEPARTAMENTO DE NUTRICIÓN, FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE

Santiago, Chile

1
Alicia Rodríguez

Associate Professor / Food Engineer / Coordinator of the Master Program in Food Sciences

Ciencias de los Alimentos y Tecnología Química

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

1
Miguel Ángel Rincón

Académico profesor asociado

INSTITUTO DE NUTRICION Y TECNOLOGIA DE LOS ALIMENTOS

Santiago, Chile

1
Sonja Buvinic

Associate Professor

Institute for Research in Dental Sciences

Universidad de Chile

Santiago, Chile

1
paula orellana

nutricionista

APS

CESFAM El Roble

santiago, Chile

1
SANDRA LOPEZ

Profesora Asistente

Nutrición

Universidad de Chile

Santiago, Chile

1
Rodrigo Troncoso

Associate Professor

Instituto de Nutrición y Tecnología de los Alimentos

Santiago, Chile

1
Lilia Masson

Presidenta

UNIVERSIDAD DE CHILE

santiago, Chile

1
Steffen Härtel

Full Professor

Instituto de Ciencias Biomédicas

University of Chile

Santiago, Chile

1
Gina Sanchez

Profesor Asociado

ICBM

UNIVERSIDAD DE CHILE, FAC MEDICINA , ICBM

Santiago, Chile

1
Manuel Estrada

/Associate Professor / PI

Physiology and Biophysics

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, INSTITUTO DE CIENCIAS BIOMÉDICAS (ICBM), PROGRAMA DE FISIOLOGÍA Y BIOFÍSICA

Santiago, Chile

1
Jaime Ortiz

Profesor Asoociado

Ciencia de los alimentos y Tecnología química

FACULTAD DE CIENCIAS QUÍMICAS Y FARMACÉUTICAS

Santiago, Chile